Restriction map for
pFN21AE1471
(Linear) (Six-base) MAPSORT of: KIEE1471.seq Check: 2473 from: 1 to: 708
KIEE1471.orf
With 182 enzymes: SgfI *
July 15, 2010 18:39 ..
AflIII A'CryG_T
Cuts at: 0 272 708
Size: 272 436
AlwNI CAG_nnn'CTG
Cuts at: 0 507 708
Size: 507 201
ApaI G_GGCC'C
Cuts at: 0 143 708
Size: 143 565
AscI GG'CGCG_CC
Cuts at: 0 178 708
Size: 178 530
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 261 294 708
Size: 261 33 414
Fragments arranged by size:
414 261 33
BanI G'GyrC_C
Cuts at: 0 245 546 585 591 708
Size: 245 301 39 6 117
Fragments arranged by size:
301 245 117 39 6
BanII G_rGCy'C
Cuts at: 0 50 143 401 607 708
Size: 50 93 258 206 101
Fragments arranged by size:
258 206 101 93 50
BbeI G_GCGC'C
Cuts at: 0 589 595 708
Size: 589 6 113
Fragments arranged by size:
589 113 6
BbsI GAAGACnn'nnnn_
Cuts at: 0 583 708
Size: 583 125
Bme1580I G_kGCm'C
Cuts at: 0 20 143 551 708
Size: 20 123 408 157
Fragments arranged by size:
408 157 123 20
BmgBI CAC'GTC
Cuts at: 0 273 708
Size: 273 435
BmrI ACTGGGnnnn_n'
Cuts at: 0 129 708
Size: 129 579
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 345 705 708
Size: 345 360 3
Fragments arranged by size:
360 345 3
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 64 708
Size: 64 644
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 34 169 708
Size: 34 135 539
Fragments arranged by size:
539 135 34
BsaHI Gr'CG_yC
Cuts at: 0 428 586 592 708
Size: 428 158 6 116
Fragments arranged by size:
428 158 116 6
BsaWI w'CCGG_w
Cuts at: 0 494 708
Size: 494 214
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 664 667 708
Size: 664 3 41
Fragments arranged by size:
664 41 3
BseYI C'CCAG_C
Cuts at: 0 19 708
Size: 19 689
BsiEI CG_ry'CG
Cuts at: 0 10 74 708
Size: 10 64 634
Fragments arranged by size:
634 64 10
BsiHKAI G_wGCw'C
Cuts at: 0 50 708
Size: 50 658
BsmBI CGTCTCn'nnnn_
Cuts at: 0 256 708
Size: 256 452
Bsp1286I G_dGCh'C
Cuts at: 0 20 50 143 401 551 607 708
Size: 20 30 93 258 150 56 101
Fragments arranged by size:
258 150 101 93 56 30 20
BspEI T'CCGG_A
Cuts at: 0 494 708
Size: 494 214
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 492 708
Size: 492 216
BsrBI CCG'CTC
Cuts at: 0 317 708
Size: 317 391
BsrFI r'CCGG_y
Cuts at: 0 69 297 588 708
Size: 69 228 291 120
Fragments arranged by size:
291 228 120 69
BsrGI T'GTAC_A
Cuts at: 0 440 708
Size: 440 268
BssHII G'CGCG_C
Cuts at: 0 178 708
Size: 178 530
BstXI CCAn_nnnn'nTGG
Cuts at: 0 558 708
Size: 558 150
EaeI y'GGCC_r
Cuts at: 0 7 128 560 708
Size: 7 121 432 148
Fragments arranged by size:
432 148 121 7
EagI C'GGCC_G
Cuts at: 0 7 708
Size: 7 701
EarI CTCTTCn'nnn_
Cuts at: 0 59 619 708
Size: 59 560 89
Fragments arranged by size:
560 89 59
EcoICRI GAG'CTC
Cuts at: 0 48 708
Size: 48 660
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 64 708
Size: 64 644
EcoNI CCTnn'n_nnAGG
Cuts at: 0 333 708
Size: 333 375
EcoO109I rG'GnC_Cy
Cuts at: 0 140 504 708
Size: 140 364 204
Fragments arranged by size:
364 204 140
HaeII r_GCGC'y
Cuts at: 0 37 589 595 708
Size: 37 552 6 113
Fragments arranged by size:
552 113 37 6
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 589 621 708
Size: 589 32 87
Fragments arranged by size:
589 87 32
KasI G'GCGC_C
Cuts at: 0 585 591 708
Size: 585 6 117
Fragments arranged by size:
585 117 6
MscI TGG'CCA
Cuts at: 0 130 708
Size: 130 578
MspA1I CmG'CkG
Cuts at: 0 665 708
Size: 665 43
NaeI GCC'GGC
Cuts at: 0 590 708
Size: 590 118
NarI GG'CG_CC
Cuts at: 0 586 592 708
Size: 586 6 116
Fragments arranged by size:
586 116 6
NgoMIV G'CCGG_C
Cuts at: 0 588 708
Size: 588 120
PflMI CCAn_nnn'nTGG
Cuts at: 0 242 708
Size: 242 466
PpuMI rG'GwC_Cy
Cuts at: 0 504 708
Size: 504 204
PspOMI G'GGCC_C
Cuts at: 0 139 708
Size: 139 569
PstI C_TGCA'G
Cuts at: 0 62 708
Size: 62 646
PvuII CAG'CTG
Cuts at: 0 665 708
Size: 665 43
SacI G_AGCT'C
Cuts at: 0 50 708
Size: 50 658
SapI GCTCTTCn'nnn_
Cuts at: 0 59 708
Size: 59 649
ScaI AGT'ACT
Cuts at: 0 568 708
Size: 568 140
SfcI C'TryA_G
Cuts at: 0 58 708
Size: 58 650
SfoI GGC'GCC
Cuts at: 0 587 593 708
Size: 587 6 115
Fragments arranged by size:
587 115 6
SgrAI Cr'CCGG_yG
Cuts at: 0 297 588 708
Size: 297 291 120
Fragments arranged by size:
297 291 120
SmlI C'TyrA_G
Cuts at: 0 49 184 708
Size: 49 135 524
Fragments arranged by size:
524 135 49
StyI C'CwwG_G
Cuts at: 0 515 581 708
Size: 515 66 127
Fragments arranged by size:
515 127 66
TatI w'GTAC_w
Cuts at: 0 440 479 566 708
Size: 440 39 87 142
Fragments arranged by size:
440 142 87 39
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 71 103 708
Size: 71 32 605
Fragments arranged by size:
605 71 32
Enzymes that do cut:
AflIII AlwNI ApaI AscI BaeI BanI BanII BbeI
BbsI Bme1580I BmgBI BmrI BplI BpmI BpuEI BsaHI
BsaWI BseRI BseYI BsiEI BsiHKAI BsmBI Bsp1286I BspEI
BspMI BsrBI BsrFI BsrGI BssHII BstXI EaeI EagI
EarI EcoICRI Eco57MI EcoNI EcoO109I HaeII Hin4I KasI
MscI MspA1I NaeI NarI NgoMIV PflMI PpuMI PspOMI
PstI PvuII SacI SapI ScaI SfcI SfoI SgrAI
SmlI StyI TatI TstI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AgeI AhdI AleI AlfI AloI ApaLI ApoI AseI
AsiSI AvaI AvrII BamHI BbvCI BcgI BciVI BclI
BfrBI BglI BglII BlpI BmtI Bpu10I BsaI BsaAI
BsaBI BsaXI BsgI BsiWI BsmI BspHI BsrDI BssSI
BstAPI BstBI BstEII BstYI BstZ17I Bsu36I BtgI BtgZI
BtsI ClaI CspCI DraI DraIII DrdI EciI EcoRI
EcoRV FalI FseI FspI FspAI HincII HindIII HpaI
KpnI MfeI MluI MmeI MslI NcoI NdeI NheI
NotI NruI NsiI NspI PacI PasI PciI PfoI
PmeI PmlI PpiI PshAI PsiI PspXI PsrI PvuI
RsrII SacII SalI SanDI SbfI SexAI SfiI SgfI
SmaI SnaBI SpeI SphI SrfI SspI StuI SwaI
TaqII Tth111I XbaI XcmI XhoI XmaI XmnI ZraI