Restriction map for
pFN21AE1466
(Linear) (Six-base) MAPSORT of: KIEE1466.seq Check: 7994 from: 1 to: 1995
KIEE1466.orf
With 182 enzymes: SgfI *
July 15, 2010 18:39 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1663 1995
Size: 1663 332
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 599 1995
Size: 599 1396
AflIII A'CryG_T
Cuts at: 0 1583 1995
Size: 1583 412
AgeI A'CCGG_T
Cuts at: 0 1272 1995
Size: 1272 723
AvaI C'yCGr_G
Cuts at: 0 1254 1995
Size: 1254 741
BanI G'GyrC_C
Cuts at: 0 299 305 583 1057 1707 1995
Size: 299 6 278 474 650 288
Fragments arranged by size:
650 474 299 288 278 6
BbeI G_GCGC'C
Cuts at: 0 303 1995
Size: 303 1692
BbsI GAAGACnn'nnnn_
Cuts at: 0 245 433 1995
Size: 245 188 1562
Fragments arranged by size:
1562 245 188
BbvCI CC'TCA_GC
Cuts at: 0 1701 1855 1995
Size: 1701 154 140
Fragments arranged by size:
1701 154 140
BglII A'GATC_T
Cuts at: 0 1356 1995
Size: 1356 639
Bme1580I G_kGCm'C
Cuts at: 0 233 1712 1995
Size: 233 1479 283
Fragments arranged by size:
1479 283 233
BmrI ACTGGGnnnn_n'
Cuts at: 0 227 1473 1995
Size: 227 1246 522
Fragments arranged by size:
1246 522 227
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1487 1995
Size: 1487 508
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 191 746 1995
Size: 191 555 1249
Fragments arranged by size:
1249 555 191
Bpu10I CC'TnA_GC
Cuts at: 0 1701 1834 1855 1995
Size: 1701 133 21 140
Fragments arranged by size:
1701 140 133 21
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 370 546 1995
Size: 370 176 1449
Fragments arranged by size:
1449 370 176
BsaI GGTCTCn'nnnn_
Cuts at: 0 1263 1864 1995
Size: 1263 601 131
Fragments arranged by size:
1263 601 131
BsaHI Gr'CG_yC
Cuts at: 0 300 1995
Size: 300 1695
BsaWI w'CCGG_w
Cuts at: 0 981 1272 1995
Size: 981 291 723
Fragments arranged by size:
981 723 291
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 744 774 863 893 1995
Size: 744 30 89 30 1102
Fragments arranged by size:
1102 744 89 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1589 1843 1872 1995
Size: 1589 254 29 123
Fragments arranged by size:
1589 254 123 29
BseYI C'CCAG_C
Cuts at: 0 35 1995
Size: 35 1960
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 205 1995
Size: 205 1790
BsiEI CG_ry'CG
Cuts at: 0 1823 1995
Size: 1823 172
BsmI GAATG_Cn'
Cuts at: 0 316 1995
Size: 316 1679
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1769 1995
Size: 1769 226
Bsp1286I G_dGCh'C
Cuts at: 0 233 1712 1995
Size: 233 1479 283
Fragments arranged by size:
1479 283 233
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 335 753 1663 1995
Size: 335 418 910 332
Fragments arranged by size:
910 418 335 332
BsrBI CCG'CTC
Cuts at: 0 1033 1995
Size: 1033 962
BsrDI GCAATG_nn'
Cuts at: 0 1380 1995
Size: 1380 615
BsrFI r'CCGG_y
Cuts at: 0 302 714 1272 1324 1995
Size: 302 412 558 52 671
Fragments arranged by size:
671 558 412 302 52
BstAPI GCAn_nnn'nTGC
Cuts at: 0 228 1995
Size: 228 1767
BstYI r'GATC_y
Cuts at: 0 1356 1995
Size: 1356 639
BtgI C'CryG_G
Cuts at: 0 1403 1664 1823 1995
Size: 1403 261 159 172
Fragments arranged by size:
1403 261 172 159
BtsI GCAGTG_nn'
Cuts at: 0 1246 1995
Size: 1246 749
DraIII CAC_nnn'GTG
Cuts at: 0 228 1995
Size: 228 1767
DrdI GACnn_nn'nnGTC
Cuts at: 0 1277 1995
Size: 1277 718
EaeI y'GGCC_r
Cuts at: 0 1210 1322 1805 1820 1995
Size: 1210 112 483 15 175
Fragments arranged by size:
1210 483 175 112 15
EagI C'GGCC_G
Cuts at: 0 1820 1995
Size: 1820 175
EarI CTCTTCn'nnn_
Cuts at: 0 1532 1922 1995
Size: 1532 390 73
Fragments arranged by size:
1532 390 73
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 191 599 746 1995
Size: 191 408 147 1249
Fragments arranged by size:
1249 408 191 147
EcoRV GAT'ATC
Cuts at: 0 1515 1995
Size: 1515 480
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1335 1995
Size: 1335 660
FspI TGC'GCA
Cuts at: 0 1568 1995
Size: 1568 427
HaeII r_GCGC'y
Cuts at: 0 303 1995
Size: 303 1692
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 493 525 863 895 1212 1244 1995
Size: 493 32 338 32 317 32 751
Fragments arranged by size:
751 493 338 317 32 32 32
KasI G'GCGC_C
Cuts at: 0 299 1995
Size: 299 1696
MfeI C'AATT_G
Cuts at: 0 689 1995
Size: 689 1306
MluI A'CGCG_T
Cuts at: 0 1583 1995
Size: 1583 412
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 455 470 1459 1901 1995
Size: 455 15 989 442 94
Fragments arranged by size:
989 455 442 94 15
MslI CAynn'nnrTG
Cuts at: 0 44 376 1995
Size: 44 332 1619
Fragments arranged by size:
1619 332 44
MspA1I CmG'CkG
Cuts at: 0 1284 1705 1741 1995
Size: 1284 421 36 254
Fragments arranged by size:
1284 421 254 36
NaeI GCC'GGC
Cuts at: 0 1326 1995
Size: 1326 669
NarI GG'CG_CC
Cuts at: 0 300 1995
Size: 300 1695
NcoI C'CATG_G
Cuts at: 0 1664 1995
Size: 1664 331
NdeI CA'TA_TG
Cuts at: 0 831 1995
Size: 831 1164
NgoMIV G'CCGG_C
Cuts at: 0 1324 1995
Size: 1324 671
PfoI T'CCnGG_A
Cuts at: 0 1346 1995
Size: 1346 649
PshAI GACnn'nnGTC
Cuts at: 0 684 1843 1995
Size: 684 1159 152
Fragments arranged by size:
1159 684 152
PstI C_TGCA'G
Cuts at: 0 609 1995
Size: 609 1386
PvuII CAG'CTG
Cuts at: 0 1741 1995
Size: 1741 254
RsrII CG'GwC_CG
Cuts at: 0 1365 1995
Size: 1365 630
SfcI C'TryA_G
Cuts at: 0 605 752 1457 1995
Size: 605 147 705 538
Fragments arranged by size:
705 605 538 147
SfoI GGC'GCC
Cuts at: 0 301 1995
Size: 301 1694
SgrAI Cr'CCGG_yG
Cuts at: 0 302 1995
Size: 302 1693
SmlI C'TyrA_G
Cuts at: 0 349 561 1995
Size: 349 212 1434
Fragments arranged by size:
1434 349 212
SspI AAT'ATT
Cuts at: 0 824 1995
Size: 824 1171
StyI C'CwwG_G
Cuts at: 0 682 1664 1995
Size: 682 982 331
Fragments arranged by size:
982 682 331
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 55 1995
Size: 55 1940
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 388 420 1995
Size: 388 32 1575
Fragments arranged by size:
1575 388 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 594 1995
Size: 594 1401
XmnI GAAnn'nnTTC
Cuts at: 0 413 1112 1995
Size: 413 699 883
Fragments arranged by size:
883 699 413
Enzymes that do cut:
AarI AcuI AflIII AgeI AvaI BanI BbeI BbsI
BbvCI BglII Bme1580I BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsmI BsmBI Bsp1286I BspMI BsrBI BsrDI BsrFI BstAPI
BstYI BtgI BtsI DraIII DrdI EaeI EagI EarI
Eco57MI EcoRV FalI FspI HaeII Hin4I KasI MfeI
MluI MmeI MslI MspA1I NaeI NarI NcoI NdeI
NgoMIV PfoI PshAI PstI PvuII RsrII SfcI SfoI
SgrAI SmlI SspI StyI TaqII TstI XcmI XmnI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflII AhdI AleI
AlfI AloI AlwNI ApaI ApaLI ApoI AscI AseI
AsiSI AvrII BaeI BamHI BanII BcgI BciVI BclI
BfrBI BglI BlpI BmgBI BmtI BsaAI BsaBI BsiHKAI
BsiWI BspEI BspHI BsrGI BssHII BssSI BstBI BstEII
BstXI BstZ17I Bsu36I BtgZI ClaI CspCI DraI EciI
EcoICRI EcoNI EcoO109I EcoRI FseI FspAI HincII HindIII
HpaI KpnI MscI NheI NotI NruI NsiI NspI
PacI PasI PciI PflMI PmeI PmlI PpiI PpuMI
PsiI PspOMI PspXI PsrI PvuI SacI SacII SalI
SanDI SapI SbfI ScaI SexAI SfiI SgfI SmaI
SnaBI SpeI SphI SrfI StuI SwaI TatI Tth111I
XbaI XhoI XmaI ZraI