(Linear) (Six-base) MAPSORT of: KIEE1463.seq  Check: 9287  from: 1  to: 450



KIEE1463.orf

 With 182 enzymes: SgfI * 

                        July 15, 2010 18:39  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0      63     450 
   Size:         63     387
 
AccI GT'mk_AC

Cuts at:      0     146     254     450 
   Size:        146     108     196

  Fragments arranged by size: 

                 196     146     108
 
AvaI C'yCGr_G

Cuts at:      0     130     450 
   Size:        130     320
 
BanI G'GyrC_C

Cuts at:      0     315     450 
   Size:        315     135
 
BanII G_rGCy'C

Cuts at:      0     156     450 
   Size:        156     294
 
Bpu10I CC'TnA_GC

Cuts at:      0     307     450 
   Size:        307     143
 
BsaWI w'CCGG_w

Cuts at:      0     428     450 
   Size:        428      22
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     280     310     450 
   Size:        280      30     140

  Fragments arranged by size: 

                 280     140      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     406     450 
   Size:        406      44
 
BsiEI CG_ry'CG

Cuts at:      0     398     450 
   Size:        398      52
 
Bsp1286I G_dGCh'C

Cuts at:      0     156     450 
   Size:        156     294
 
BspEI T'CCGG_A

Cuts at:      0     428     450 
   Size:        428      22
 
BspHI T'CATG_A

Cuts at:      0     222     450 
   Size:        222     228
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0      63     450 
   Size:         63     387
 
BsrBI CCG'CTC

Cuts at:      0     114     450 
   Size:        114     336
 
BsrFI r'CCGG_y

Cuts at:      0     121     208     450 
   Size:        121      87     242

  Fragments arranged by size: 

                 242     121      87
 
BssHII G'CGCG_C

Cuts at:      0      85     265     292     332     334     336     405     450 
   Size:         85     180      27      40       2       2      69      45

  Fragments arranged by size: 

                 180      85      69      45      40      27       2       2
            
 
BtgI C'CryG_G

Cuts at:      0     442     450 
   Size:        442       8
 
EaeI y'GGCC_r

Cuts at:      0     395     450 
   Size:        395      55
 
EagI C'GGCC_G

Cuts at:      0     395     450 
   Size:        395      55
 
EcoO109I rG'GnC_Cy

Cuts at:      0      92     450 
   Size:         92     358
 
HaeII r_GCGC'y

Cuts at:      0     361     450 
   Size:        361      89
 
MspA1I CmG'CkG

Cuts at:      0     183     321     450 
   Size:        183     138     129

  Fragments arranged by size: 

                 183     138     129
 
NaeI GCC'GGC

Cuts at:      0     210     450 
   Size:        210     240
 
NgoMIV G'CCGG_C

Cuts at:      0     208     450 
   Size:        208     242
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     200     450 
   Size:        200     250
 
PstI C_TGCA'G

Cuts at:      0      74     169     450 
   Size:         74      95     281

  Fragments arranged by size: 

                 281      95      74
 
SbfI CC_TGCA'GG

Cuts at:      0      74     450 
   Size:         74     376
 
ScaI AGT'ACT

Cuts at:      0     175     450 
   Size:        175     275
 
SfcI C'TryA_G

Cuts at:      0      70     165     450 
   Size:         70      95     285

  Fragments arranged by size: 

                 285      95      70
 
TatI w'GTAC_w

Cuts at:      0     173     450 
   Size:        173     277
 

 Enzymes that do cut:

     AarI     AccI     AvaI     BanI    BanII   Bpu10I    BsaWI    BsaXI
    BseRI    BsiEI Bsp1286I    BspEI    BspHI    BspMI    BsrBI    BsrFI
   BssHII     BtgI     EaeI     EagI EcoO109I    HaeII   MspA1I     NaeI
   NgoMIV    PflMI     PstI     SbfI     ScaI     SfcI     TatI

 Enzymes that do not cut: 

    AatII   Acc65I     AclI     AcuI     AfeI    AflII   AflIII     AgeI
     AhdI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI     ApoI
     AscI     AseI    AsiSI    AvrII     BaeI    BamHI     BbeI     BbsI
    BbvCI     BcgI    BciVI     BclI    BfrBI     BglI    BglII     BlpI
 Bme1580I    BmgBI     BmrI     BmtI     BplI     BpmI    BpuEI     BsaI
    BsaAI    BsaBI    BsaHI    BseYI     BsgI  BsiHKAI    BsiWI     BsmI
    BsmBI    BsrDI    BsrGI    BssSI   BstAPI    BstBI   BstEII    BstXI
    BstYI  BstZ17I   Bsu36I    BtgZI     BtsI     ClaI    CspCI     DraI
   DraIII     DrdI     EarI     EciI  EcoICRI  Eco57MI    EcoNI    EcoRI
    EcoRV     FalI     FseI     FspI    FspAI    Hin4I   HincII  HindIII
     HpaI     KasI     KpnI     MfeI     MluI     MmeI     MscI     MslI
     NarI     NcoI     NdeI     NheI     NotI     NruI     NsiI     NspI
     PacI     PasI     PciI     PfoI     PmeI     PmlI     PpiI    PpuMI
    PshAI     PsiI   PspOMI    PspXI     PsrI     PvuI    PvuII    RsrII
     SacI    SacII     SalI    SanDI     SapI    SexAI     SfiI     SfoI
     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI     SrfI
     SspI     StuI     StyI     SwaI    TaqII     TstI  Tth111I     XbaI
     XcmI     XhoI     XmaI     XmnI     ZraI