Restriction map for
pFN21AE1458
(Linear) (Six-base) MAPSORT of: KIEE1458.seq Check: 6898 from: 1 to: 813
KIEE1458.orf
With 182 enzymes: SgfI *
July 15, 2010 18:39 ..
AccI GT'mk_AC
Cuts at: 0 569 813
Size: 569 244
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 322 449 811 813
Size: 322 127 362 2
Fragments arranged by size:
362 322 127 2
AleI CACnn'nnGTG
Cuts at: 0 364 813
Size: 364 449
ApoI r'AATT_y
Cuts at: 0 289 409 813
Size: 289 120 404
Fragments arranged by size:
404 289 120
AseI AT'TA_AT
Cuts at: 0 479 813
Size: 479 334
AvaI C'yCGr_G
Cuts at: 0 180 194 813
Size: 180 14 619
Fragments arranged by size:
619 180 14
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 160 193 813
Size: 160 33 620
Fragments arranged by size:
620 160 33
BbsI GAAGACnn'nnnn_
Cuts at: 0 498 813
Size: 498 315
BglI GCCn_nnn'nGGC
Cuts at: 0 740 813
Size: 740 73
BglII A'GATC_T
Cuts at: 0 415 548 813
Size: 415 133 265
Fragments arranged by size:
415 265 133
BmrI ACTGGGnnnn_n'
Cuts at: 0 270 813
Size: 270 543
BmtI G_CTAG'C
Cuts at: 0 22 813
Size: 22 791
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 116 813
Size: 116 697
Bpu10I CC'TnA_GC
Cuts at: 0 226 813
Size: 226 587
BsaI GGTCTCn'nnnn_
Cuts at: 0 607 813
Size: 607 206
BsaWI w'CCGG_w
Cuts at: 0 33 813
Size: 33 780
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 289 319 813
Size: 289 30 494
Fragments arranged by size:
494 289 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 136 160 813
Size: 136 24 653
Fragments arranged by size:
653 136 24
BseYI C'CCAG_C
Cuts at: 0 110 813
Size: 110 703
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 124 205 813
Size: 124 81 608
Fragments arranged by size:
608 124 81
BsiEI CG_ry'CG
Cuts at: 0 45 206 813
Size: 45 161 607
Fragments arranged by size:
607 161 45
BsmI GAATG_Cn'
Cuts at: 0 700 813
Size: 700 113
BspEI T'CCGG_A
Cuts at: 0 33 813
Size: 33 780
BsrFI r'CCGG_y
Cuts at: 0 90 813
Size: 90 723
BstYI r'GATC_y
Cuts at: 0 415 548 774 813
Size: 415 133 226 39
Fragments arranged by size:
415 226 133 39
BstZ17I GTA'TAC
Cuts at: 0 570 813
Size: 570 243
BtgI C'CryG_G
Cuts at: 0 39 813
Size: 39 774
ClaI AT'CG_AT
Cuts at: 0 281 813
Size: 281 532
EaeI y'GGCC_r
Cuts at: 0 42 813
Size: 42 771
EagI C'GGCC_G
Cuts at: 0 42 813
Size: 42 771
EarI CTCTTCn'nnn_
Cuts at: 0 137 610 813
Size: 137 473 203
Fragments arranged by size:
473 203 137
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 116 322 449 811 813
Size: 116 206 127 362 2
Fragments arranged by size:
362 206 127 116 2
EcoO109I rG'GnC_Cy
Cuts at: 0 128 813
Size: 128 685
EcoRI G'AATT_C
Cuts at: 0 409 813
Size: 409 404
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 520 813
Size: 520 293
HaeII r_GCGC'y
Cuts at: 0 10 256 813
Size: 10 246 557
Fragments arranged by size:
557 246 10
HincII GTy'rAC
Cuts at: 0 308 813
Size: 308 505
HpaI GTT'AAC
Cuts at: 0 308 813
Size: 308 505
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 397 813
Size: 397 416
MslI CAynn'nnrTG
Cuts at: 0 364 366 813
Size: 364 2 447
Fragments arranged by size:
447 364 2
MspA1I CmG'CkG
Cuts at: 0 15 274 781 813
Size: 15 259 507 32
Fragments arranged by size:
507 259 32 15
NaeI GCC'GGC
Cuts at: 0 92 813
Size: 92 721
NgoMIV G'CCGG_C
Cuts at: 0 90 813
Size: 90 723
NheI G'CTAG_C
Cuts at: 0 18 813
Size: 18 795
NruI TCG'CGA
Cuts at: 0 140 813
Size: 140 673
NspI r_CATG'y
Cuts at: 0 443 813
Size: 443 370
PvuII CAG'CTG
Cuts at: 0 274 781 813
Size: 274 507 32
Fragments arranged by size:
507 274 32
SexAI A'CCwGG_T
Cuts at: 0 750 813
Size: 750 63
SmaI CCC'GGG
Cuts at: 0 182 196 813
Size: 182 14 617
Fragments arranged by size:
617 182 14
SphI G_CATG'C
Cuts at: 0 443 813
Size: 443 370
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 192 813
Size: 192 621
TatI w'GTAC_w
Cuts at: 0 707 813
Size: 707 106
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 260 292 813
Size: 260 32 521
Fragments arranged by size:
521 260 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 257 813
Size: 257 556
XmaI C'CCGG_G
Cuts at: 0 180 194 813
Size: 180 14 619
Fragments arranged by size:
619 180 14
XmnI GAAnn'nnTTC
Cuts at: 0 796 813
Size: 796 17
Enzymes that do cut:
AccI AcuI AleI ApoI AseI AvaI BaeI BbsI
BglI BglII BmrI BmtI BpmI Bpu10I BsaI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsmI BspEI BsrFI
BstYI BstZ17I BtgI ClaI EaeI EagI EarI Eco57MI
EcoO109I EcoRI FalI HaeII HincII HpaI MmeI MslI
MspA1I NaeI NgoMIV NheI NruI NspI PvuII SexAI
SmaI SphI TaqII TatI TstI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AflIII AgeI
AhdI AlfI AloI AlwNI ApaI ApaLI AscI AsiSI
AvrII BamHI BanI BanII BbeI BbvCI BcgI BciVI
BclI BfrBI BlpI Bme1580I BmgBI BplI BpuEI BsaAI
BsaBI BsaHI BsiHKAI BsiWI BsmBI Bsp1286I BspHI BspMI
BsrBI BsrDI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI Bsu36I BtgZI BtsI CspCI DraI DraIII DrdI
EciI EcoICRI EcoNI EcoRV FseI FspI FspAI Hin4I
HindIII KasI KpnI MfeI MluI MscI NarI NcoI
NdeI NotI NsiI PacI PasI PciI PflMI PfoI
PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI
PsrI PstI PvuI RsrII SacI SacII SalI SanDI
SapI SbfI ScaI SfcI SfiI SfoI SgfI SgrAI
SmlI SnaBI SpeI SrfI SspI StuI StyI SwaI
Tth111I XbaI XhoI ZraI