Restriction map for
pFN21AE1453
(Linear) (Six-base) MAPSORT of: KIEE1453.seq Check: 4597 from: 1 to: 3186
KIEE1453.orf
With 182 enzymes: SgfI *
July 15, 2010 18:39 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1270 3186
Size: 1270 1916
AatII G_ACGT'C
Cuts at: 0 1387 3186
Size: 1387 1799
AccI GT'mk_AC
Cuts at: 0 140 1904 3186
Size: 140 1764 1282
Fragments arranged by size:
1764 1282 140
Acc65I G'GTAC_C
Cuts at: 0 2678 3186
Size: 2678 508
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 771 1243 3186
Size: 771 472 1943
Fragments arranged by size:
1943 771 472
AfeI AGC'GCT
Cuts at: 0 376 3186
Size: 376 2810
AflIII A'CryG_T
Cuts at: 0 3019 3186
Size: 3019 167
AleI CACnn'nnGTG
Cuts at: 0 625 2616 3186
Size: 625 1991 570
Fragments arranged by size:
1991 625 570
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1913 3186
Size: 1913 1273
AlwNI CAG_nnn'CTG
Cuts at: 0 197 794 1371 1611 1812 2436 3186
Size: 197 597 577 240 201 624 750
Fragments arranged by size:
750 624 597 577 240 201 197
ApaI G_GGCC'C
Cuts at: 0 611 763 1020 3186
Size: 611 152 257 2166
Fragments arranged by size:
2166 611 257 152
ApaLI G'TGCA_C
Cuts at: 0 1212 3186
Size: 1212 1974
ApoI r'AATT_y
Cuts at: 0 712 3186
Size: 712 2474
AvaI C'yCGr_G
Cuts at: 0 604 1417 2362 2683 3186
Size: 604 813 945 321 503
Fragments arranged by size:
945 813 604 503 321
BamHI G'GATC_C
Cuts at: 0 1034 2150 3186
Size: 1034 1116 1036
Fragments arranged by size:
1116 1036 1034
BanI G'GyrC_C
Cuts at: 0 939 2678 3031 3186
Size: 939 1739 353 155
Fragments arranged by size:
1739 939 353 155
BanII G_rGCy'C
Cuts at: 0 116 611 749 763 1020 1186 3186
Size: 116 495 138 14 257 166 2000
Fragments arranged by size:
2000 495 257 166 138 116 14
BbsI GAAGACnn'nnnn_
Cuts at: 0 499 777 2921 3186
Size: 499 278 2144 265
Fragments arranged by size:
2144 499 278 265
BbvCI CC'TCA_GC
Cuts at: 0 733 1510 2932 3186
Size: 733 777 1422 254
Fragments arranged by size:
1422 777 733 254
BclI T'GATC_A
Cuts at: 0 86 2040 3186
Size: 86 1954 1146
Fragments arranged by size:
1954 1146 86
BglI GCCn_nnn'nGGC
Cuts at: 0 758 3186
Size: 758 2428
BlpI GC'TnA_GC
Cuts at: 0 52 748 1348 3186
Size: 52 696 600 1838
Fragments arranged by size:
1838 696 600 52
Bme1580I G_kGCm'C
Cuts at: 0 611 763 944 1020 1216 3186
Size: 611 152 181 76 196 1970
Fragments arranged by size:
1970 611 196 181 152 76
BmgBI CAC'GTC
Cuts at: 0 357 2441 3186
Size: 357 2084 745
Fragments arranged by size:
2084 745 357
BmrI ACTGGGnnnn_n'
Cuts at: 0 207 696 930 3186
Size: 207 489 234 2256
Fragments arranged by size:
2256 489 234 207
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1480 3186
Size: 1480 1706
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 64 100 202 391 548 1099 1570 2173
Size: 64 36 102 189 157 551 471 603
Cuts at: 2173 3186
Size: 1013
Fragments arranged by size:
1013 603 551 471 189 157 102 64
36
Bpu10I CC'TnA_GC
Cuts at: 0 733 1510 2932 3186
Size: 733 777 1422 254
Fragments arranged by size:
1422 777 733 254
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2352 2694 2886 3186
Size: 2352 342 192 300
Fragments arranged by size:
2352 342 300 192
BsaBI GATnn'nnATC
Cuts at: 0 574 2228 3186
Size: 574 1654 958
Fragments arranged by size:
1654 958 574
BsaHI Gr'CG_yC
Cuts at: 0 1384 2015 3186
Size: 1384 631 1171
Fragments arranged by size:
1384 1171 631
BsaWI w'CCGG_w
Cuts at: 0 1031 1618 1734 3038 3186
Size: 1031 587 116 1304 148
Fragments arranged by size:
1304 1031 587 148 116
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 98 128 1465 1495 3186
Size: 98 30 1337 30 1691
Fragments arranged by size:
1691 1337 98 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1060 1096 1170 1573 1855 1858 3186
Size: 1060 36 74 403 282 3 1328
Fragments arranged by size:
1328 1060 403 282 74 36 3
BseYI C'CCAG_C
Cuts at: 0 876 1179 2217 3186
Size: 876 303 1038 969
Fragments arranged by size:
1038 969 876 303
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 498 786 1444 1873 3186
Size: 498 288 658 429 1313
Fragments arranged by size:
1313 658 498 429 288
BsiHKAI G_wGCw'C
Cuts at: 0 116 749 1216 3186
Size: 116 633 467 1970
Fragments arranged by size:
1970 633 467 116
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1112 3186
Size: 1112 2074
Bsp1286I G_dGCh'C
Cuts at: 0 116 611 749 763 944 1020 1186 1216
Size: 116 495 138 14 181 76 166 30
Cuts at: 1216 3186
Size: 1970
Fragments arranged by size:
1970 495 181 166 138 116 76 30
14
BspEI T'CCGG_A
Cuts at: 0 1031 1618 3038 3186
Size: 1031 587 1420 148
Fragments arranged by size:
1420 1031 587 148
BspHI T'CATG_A
Cuts at: 0 3171 3179 3186
Size: 3171 8 7
Fragments arranged by size:
3171 8 7
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 38 470 1270 1811 1955 1956 2360 3186
Size: 38 432 800 541 144 1 404 826
Fragments arranged by size:
826 800 541 432 404 144 38 1
BsrDI GCAATG_nn'
Cuts at: 0 2298 3186
Size: 2298 888
BsrFI r'CCGG_y
Cuts at: 0 936 1304 3186
Size: 936 368 1882
Fragments arranged by size:
1882 936 368
BsrGI T'GTAC_A
Cuts at: 0 2492 3186
Size: 2492 694
BssHII G'CGCG_C
Cuts at: 0 1334 3186
Size: 1334 1852
BssSI C'ACGA_G
Cuts at: 0 109 3186
Size: 109 3077
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1812 3186
Size: 1812 1374
BstEII G'GTnAC_C
Cuts at: 0 999 3186
Size: 999 2187
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1704 3186
Size: 1704 1482
BstYI r'GATC_y
Cuts at: 0 122 246 1034 1404 2029 2097 2150 2790
Size: 122 124 788 370 625 68 53 640
Cuts at: 2790 3186
Size: 396
Fragments arranged by size:
788 640 625 396 370 124 122 68
53
Bsu36I CC'TnA_GG
Cuts at: 0 1021 3186
Size: 1021 2165
BtgI C'CryG_G
Cuts at: 0 1193 3186
Size: 1193 1993
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1773 2747 3186
Size: 1773 974 439
Fragments arranged by size:
1773 974 439
BtsI GCAGTG_nn'
Cuts at: 0 1529 1960 1997 2401 3186
Size: 1529 431 37 404 785
Fragments arranged by size:
1529 785 431 404 37
DraI TTT'AAA
Cuts at: 0 447 3186
Size: 447 2739
EaeI y'GGCC_r
Cuts at: 0 1358 1578 2624 3186
Size: 1358 220 1046 562
Fragments arranged by size:
1358 1046 562 220
EarI CTCTTCn'nnn_
Cuts at: 0 511 1559 1684 2255 3186
Size: 511 1048 125 571 931
Fragments arranged by size:
1048 931 571 511 125
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1057 1456 3186
Size: 1057 399 1730
Fragments arranged by size:
1730 1057 399
EcoICRI GAG'CTC
Cuts at: 0 114 747 3186
Size: 114 633 2439
Fragments arranged by size:
2439 633 114
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 64 100 202 391 548 771 1099 1243
Size: 64 36 102 189 157 223 328 144
Cuts at: 1243 1570 2173 3186
Size: 327 603 1013
Fragments arranged by size:
1013 603 328 327 223 189 157 144
102 64 36
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1042 1081 1468 1552 2836 3186
Size: 1042 39 387 84 1284 350
Fragments arranged by size:
1284 1042 387 350 84 39
EcoO109I rG'GnC_Cy
Cuts at: 0 608 1016 1017 1026 1094 1248 1368 2574
Size: 608 408 1 9 68 154 120 1206
Cuts at: 2574 3186
Size: 612
Fragments arranged by size:
1206 612 608 408 154 120 68 9
1
EcoRV GAT'ATC
Cuts at: 0 2359 3186
Size: 2359 827
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 3182 3186
Size: 3182 4
HaeII r_GCGC'y
Cuts at: 0 378 898 3186
Size: 378 520 2288
Fragments arranged by size:
2288 520 378
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1465 1497 2879 2908 2911 2940 3186
Size: 1465 32 1382 29 3 29 246
Fragments arranged by size:
1465 1382 246 32 29 29 3
HincII GTy'rAC
Cuts at: 0 2427 3186
Size: 2427 759
HindIII A'AGCT_T
Cuts at: 0 2755 3186
Size: 2755 431
KpnI G_GTAC'C
Cuts at: 0 2682 3186
Size: 2682 504
MfeI C'AATT_G
Cuts at: 0 2565 3186
Size: 2565 621
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1457 1685 2480 2519 3186
Size: 1457 228 795 39 667
Fragments arranged by size:
1457 795 667 228 39
MscI TGG'CCA
Cuts at: 0 2626 3186
Size: 2626 560
MslI CAynn'nnrTG
Cuts at: 0 625 1063 1895 2616 3186
Size: 625 438 832 721 570
Fragments arranged by size:
832 721 625 570 438
MspA1I CmG'CkG
Cuts at: 0 737 1179 1195 1326 2221 2936 3186
Size: 737 442 16 131 895 715 250
Fragments arranged by size:
895 737 715 442 250 131 16
NspI r_CATG'y
Cuts at: 0 2052 3023 3186
Size: 2052 971 163
Fragments arranged by size:
2052 971 163
PasI CC'CwG_GG
Cuts at: 0 593 1013 3149 3186
Size: 593 420 2136 37
Fragments arranged by size:
2136 593 420 37
PciI A'CATG_T
Cuts at: 0 3019 3186
Size: 3019 167
PflMI CCAn_nnn'nTGG
Cuts at: 0 552 2196 3186
Size: 552 1644 990
Fragments arranged by size:
1644 990 552
PfoI T'CCnGG_A
Cuts at: 0 41 503 1076 1448 1985 3186
Size: 41 462 573 372 537 1201
Fragments arranged by size:
1201 573 537 462 372 41
PpuMI rG'GwC_Cy
Cuts at: 0 1026 1248 2574 3186
Size: 1026 222 1326 612
Fragments arranged by size:
1326 1026 612 222
PshAI GACnn'nnGTC
Cuts at: 0 1439 2180 2966 3186
Size: 1439 741 786 220
Fragments arranged by size:
1439 786 741 220
PspOMI G'GGCC_C
Cuts at: 0 607 759 1016 3186
Size: 607 152 257 2170
Fragments arranged by size:
2170 607 257 152
PstI C_TGCA'G
Cuts at: 0 35 645 742 1232 1303 1808 1952 1967
Size: 35 610 97 490 71 505 144 15
Cuts at: 1967 2528 2699 3186
Size: 561 171 487
Fragments arranged by size:
610 561 505 490 487 171 144 97
71 35 15
PvuII CAG'CTG
Cuts at: 0 737 1179 1326 2221 2936 3186
Size: 737 442 147 895 715 250
Fragments arranged by size:
895 737 715 442 250 147
SacI G_AGCT'C
Cuts at: 0 116 749 3186
Size: 116 633 2437
Fragments arranged by size:
2437 633 116
SacII CC_GC'GG
Cuts at: 0 1196 3186
Size: 1196 1990
SapI GCTCTTCn'nnn_
Cuts at: 0 511 2255 3186
Size: 511 1744 931
Fragments arranged by size:
1744 931 511
SbfI CC_TGCA'GG
Cuts at: 0 35 645 1808 3186
Size: 35 610 1163 1378
Fragments arranged by size:
1378 1163 610 35
SfcI C'TryA_G
Cuts at: 0 31 641 738 1228 1299 1804 1948 1963
Size: 31 610 97 490 71 505 144 15
Cuts at: 1963 2524 2695 3186
Size: 561 171 491
Fragments arranged by size:
610 561 505 491 490 171 144 97
71 31 15
SmaI CCC'GGG
Cuts at: 0 606 3186
Size: 606 2580
SmlI C'TyrA_G
Cuts at: 0 2331 2673 2865 3186
Size: 2331 342 192 321
Fragments arranged by size:
2331 342 321 192
StuI AGG'CCT
Cuts at: 0 647 3162 3186
Size: 647 2515 24
Fragments arranged by size:
2515 647 24
StyI C'CwwG_G
Cuts at: 0 648 983 1697 2411 2702 2978 3186
Size: 648 335 714 714 291 276 208
Fragments arranged by size:
714 714 648 335 291 276 208
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 189 196 3186
Size: 189 7 2990
Fragments arranged by size:
2990 189 7
TatI w'GTAC_w
Cuts at: 0 2492 3186
Size: 2492 694
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 98 130 2142 2174 3186
Size: 98 32 2012 32 1012
Fragments arranged by size:
2012 1012 98 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 1252 1477 3186
Size: 1252 225 1709
Fragments arranged by size:
1709 1252 225
XbaI T'CTAG_A
Cuts at: 0 351 3186
Size: 351 2835
XmaI C'CCGG_G
Cuts at: 0 604 3186
Size: 604 2582
ZraI GAC'GTC
Cuts at: 0 1385 3186
Size: 1385 1801
Enzymes that do cut:
AarI AatII AccI Acc65I AcuI AfeI AflIII AleI
AlfI AlwNI ApaI ApaLI ApoI AvaI BamHI BanI
BanII BbsI BbvCI BclI BglI BlpI Bme1580I BmgBI
BmrI BplI BpmI Bpu10I BpuEI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiHKAI BsmBI Bsp1286I BspEI
BspHI BspMI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI DraI
EaeI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRV
FalI HaeII Hin4I HincII HindIII KpnI MfeI MmeI
MscI MslI MspA1I NspI PasI PciI PflMI PfoI
PpuMI PshAI PspOMI PstI PvuII SacI SacII SapI
SbfI SfcI SmaI SmlI StuI StyI TaqII TatI
TstI Tth111I XbaI XmaI ZraI
Enzymes that do not cut:
AclI AflII AgeI AhdI AloI AscI AseI AsiSI
AvrII BaeI BbeI BcgI BciVI BfrBI BglII BmtI
BsaI BsaAI BsiEI BsiWI BsmI BsrBI BstBI BstZ17I
ClaI CspCI DraIII DrdI EagI EcoRI FseI FspI
FspAI HpaI KasI MluI NaeI NarI NcoI NdeI
NgoMIV NheI NotI NruI NsiI PacI PmeI PmlI
PpiI PsiI PspXI PsrI PvuI RsrII SalI SanDI
ScaI SexAI SfiI SfoI SgfI SgrAI SnaBI SpeI
SphI SrfI SspI SwaI XcmI XhoI XmnI