Restriction map for
pFN21AE1452
(Linear) (Six-base) MAPSORT of: KIEE1452.seq Check: 7427 from: 1 to: 747
KIEE1452.orf
With 182 enzymes: SgfI *
July 15, 2010 18:39 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 190 712 747
Size: 190 522 35
Fragments arranged by size:
522 190 35
AfeI AGC'GCT
Cuts at: 0 346 747
Size: 346 401
AlwNI CAG_nnn'CTG
Cuts at: 0 196 747
Size: 196 551
ApaLI G'TGCA_C
Cuts at: 0 601 747
Size: 601 146
AvaI C'yCGr_G
Cuts at: 0 22 85 747
Size: 22 63 662
Fragments arranged by size:
662 63 22
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 48 81 747
Size: 48 33 666
Fragments arranged by size:
666 48 33
BbsI GAAGACnn'nnnn_
Cuts at: 0 62 257 290 747
Size: 62 195 33 457
Fragments arranged by size:
457 195 62 33
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 400 434 747
Size: 400 34 313
Fragments arranged by size:
400 313 34
BfrBI ATG'CAT
Cuts at: 0 124 747
Size: 124 623
Bme1580I G_kGCm'C
Cuts at: 0 605 747
Size: 605 142
BmrI ACTGGGnnnn_n'
Cuts at: 0 184 301 747
Size: 184 117 446
Fragments arranged by size:
446 184 117
Bpu10I CC'TnA_GC
Cuts at: 0 10 747
Size: 10 737
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 180 694 747
Size: 180 514 53
Fragments arranged by size:
514 180 53
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 437 451 467 481 747
Size: 437 14 16 14 266
Fragments arranged by size:
437 266 16 14 14
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 238 747
Size: 238 509
BsiHKAI G_wGCw'C
Cuts at: 0 605 747
Size: 605 142
Bsp1286I G_dGCh'C
Cuts at: 0 605 747
Size: 605 142
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 89 747
Size: 89 658
BsrBI CCG'CTC
Cuts at: 0 228 747
Size: 228 519
BsrDI GCAATG_nn'
Cuts at: 0 324 666 747
Size: 324 342 81
Fragments arranged by size:
342 324 81
BssSI C'ACGA_G
Cuts at: 0 604 747
Size: 604 143
BstAPI GCAn_nnn'nTGC
Cuts at: 0 600 747
Size: 600 147
BstEII G'GTnAC_C
Cuts at: 0 390 747
Size: 390 357
BstYI r'GATC_y
Cuts at: 0 653 747
Size: 653 94
BtsI GCAGTG_nn'
Cuts at: 0 557 747
Size: 557 190
DraI TTT'AAA
Cuts at: 0 131 747
Size: 131 616
EaeI y'GGCC_r
Cuts at: 0 2 747
Size: 2 745
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 190 712 747
Size: 190 522 35
Fragments arranged by size:
522 190 35
EcoO109I rG'GnC_Cy
Cuts at: 0 174 292 747
Size: 174 118 455
Fragments arranged by size:
455 174 118
HaeII r_GCGC'y
Cuts at: 0 348 747
Size: 348 399
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 437 469 747
Size: 437 32 278
Fragments arranged by size:
437 278 32
MscI TGG'CCA
Cuts at: 0 4 747
Size: 4 743
MslI CAynn'nnrTG
Cuts at: 0 190 581 747
Size: 190 391 166
Fragments arranged by size:
391 190 166
MspA1I CmG'CkG
Cuts at: 0 408 597 747
Size: 408 189 150
Fragments arranged by size:
408 189 150
NsiI A_TGCA'T
Cuts at: 0 126 747
Size: 126 621
PasI CC'CwG_GG
Cuts at: 0 719 747
Size: 719 28
PfoI T'CCnGG_A
Cuts at: 0 455 747
Size: 455 292
PpuMI rG'GwC_Cy
Cuts at: 0 292 747
Size: 292 455
PshAI GACnn'nnGTC
Cuts at: 0 290 747
Size: 290 457
PstI C_TGCA'G
Cuts at: 0 344 413 747
Size: 344 69 334
Fragments arranged by size:
344 334 69
PvuII CAG'CTG
Cuts at: 0 408 597 747
Size: 408 189 150
Fragments arranged by size:
408 189 150
SexAI A'CCwGG_T
Cuts at: 0 386 397 747
Size: 386 11 350
Fragments arranged by size:
386 350 11
SfcI C'TryA_G
Cuts at: 0 340 409 747
Size: 340 69 338
Fragments arranged by size:
340 338 69
SmlI C'TyrA_G
Cuts at: 0 159 709 747
Size: 159 550 38
Fragments arranged by size:
550 159 38
StyI C'CwwG_G
Cuts at: 0 35 747
Size: 35 712
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 571 747
Size: 571 176
XmnI GAAnn'nnTTC
Cuts at: 0 697 747
Size: 697 50
Enzymes that do cut:
AcuI AfeI AlwNI ApaLI AvaI BaeI BbsI BcgI
BfrBI Bme1580I BmrI Bpu10I BpuEI BsaXI BseRI BsiHKAI
Bsp1286I BspMI BsrBI BsrDI BssSI BstAPI BstEII BstYI
BtsI DraI EaeI Eco57MI EcoO109I HaeII Hin4I MscI
MslI MspA1I NsiI PasI PfoI PpuMI PshAI PstI
PvuII SexAI SfcI SmlI StyI TaqII XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AflII AflIII AgeI
AhdI AleI AlfI AloI ApaI ApoI AscI AseI
AsiSI AvrII BamHI BanI BanII BbeI BbvCI BciVI
BclI BglI BglII BlpI BmgBI BmtI BplI BpmI
BsaI BsaAI BsaBI BsaHI BsaWI BseYI BsgI BsiEI
BsiWI BsmI BsmBI BspEI BspHI BsrFI BsrGI BssHII
BstBI BstXI BstZ17I Bsu36I BtgI BtgZI ClaI CspCI
DraIII DrdI EagI EarI EciI EcoICRI EcoNI EcoRI
EcoRV FalI FseI FspI FspAI HincII HindIII HpaI
KasI KpnI MfeI MluI MmeI NaeI NarI NcoI
NdeI NgoMIV NheI NotI NruI NspI PacI PciI
PflMI PmeI PmlI PpiI PsiI PspOMI PspXI PsrI
PvuI RsrII SacI SacII SalI SanDI SapI SbfI
ScaI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI
SphI SrfI SspI StuI SwaI TatI TstI Tth111I
XbaI XcmI XhoI XmaI ZraI