(Linear) (Six-base) MAPSORT of: KIEE1447.seq  Check: 1130  from: 1  to: 876



KIEE1447.orf

 With 182 enzymes: SgfI * 

                        July 15, 2010 18:39  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     178     876 
   Size:        178     698
 
Acc65I G'GTAC_C

Cuts at:      0     487     876 
   Size:        487     389
 
AflII C'TTAA_G

Cuts at:      0     637     876 
   Size:        637     239
 
AhdI GACnn_n'nnGTC

Cuts at:      0     660     876 
   Size:        660     216
 
AlwNI CAG_nnn'CTG

Cuts at:      0     533     621     876 
   Size:        533      88     255

  Fragments arranged by size: 

                 533     255      88
 
ApoI r'AATT_y

Cuts at:      0     100     206     466     808     876 
   Size:        100     106     260     342      68

  Fragments arranged by size: 

                 342     260     106     100      68
 
AvaI C'yCGr_G

Cuts at:      0     322     876 
   Size:        322     554
 
AvrII C'CTAG_G

Cuts at:      0     630     876 
   Size:        630     246
 
BanI G'GyrC_C

Cuts at:      0     487     876 
   Size:        487     389
 
BglII A'GATC_T

Cuts at:      0     792     876 
   Size:        792      84
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     704     876 
   Size:        704     172
 
BsaWI w'CCGG_w

Cuts at:      0      56     876 
   Size:         56     820
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      30     876 
   Size:         30     846
 
BseYI C'CCAG_C

Cuts at:      0     453     533     876 
   Size:        453      80     343

  Fragments arranged by size: 

                 453     343      80
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     206     876 
   Size:        206     670
 
BspEI T'CCGG_A

Cuts at:      0      56     876 
   Size:         56     820
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     178     722     748     876 
   Size:        178     544      26     128

  Fragments arranged by size: 

                 544     178     128      26
 
BstYI r'GATC_y

Cuts at:      0     792     876 
   Size:        792      84
 
BtgI C'CryG_G

Cuts at:      0      33      77     668     876 
   Size:         33      44     591     208

  Fragments arranged by size: 

                 591     208      44      33
 
BtsI GCAGTG_nn'

Cuts at:      0     534     876 
   Size:        534     342
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     194     876 
   Size:        194     682
 
EcoRI G'AATT_C

Cuts at:      0     100     876 
   Size:        100     776
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     426     876 
   Size:        426     450
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     136     168     230     262     343     375     646     678 
   Size:        136      32      62      32      81      32     271      32

Cuts at:    678     876 
   Size:        198

  Fragments arranged by size: 

                 271     198     136      81      62      32      32      32
                  32
 
KpnI G_GTAC'C

Cuts at:      0     491     876 
   Size:        491     385
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     362     876 
   Size:        362     514
 
MslI CAynn'nnrTG

Cuts at:      0     616     876 
   Size:        616     260
 
NcoI C'CATG_G

Cuts at:      0     668     876 
   Size:        668     208
 
NdeI CA'TA_TG

Cuts at:      0     419     876 
   Size:        419     457
 
NspI r_CATG'y

Cuts at:      0      96     876 
   Size:         96     780
 
SmlI C'TyrA_G

Cuts at:      0     322     637     876 
   Size:        322     315     239

  Fragments arranged by size: 

                 322     315     239
 
SphI G_CATG'C

Cuts at:      0      96     876 
   Size:         96     780
 
StyI C'CwwG_G

Cuts at:      0     630     668     876 
   Size:        630      38     208

  Fragments arranged by size: 

                 630     208      38
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0      73     105     529     561     876 
   Size:         73      32     424      32     315

  Fragments arranged by size: 

                 424     315      73      32      32
 
XhoI C'TCGA_G

Cuts at:      0     322     876 
   Size:        322     554
 
XmnI GAAnn'nnTTC

Cuts at:      0     469     876 
   Size:        469     407
 

 Enzymes that do cut:

     AarI   Acc65I    AflII     AhdI    AlwNI     ApoI     AvaI    AvrII
     BanI    BglII     BsaI    BsaWI    BseRI    BseYI     BsgI    BspEI
    BspMI    BstYI     BtgI     BtsI     EciI    EcoRI     FalI    Hin4I
     KpnI     MmeI     MslI     NcoI     NdeI     NspI     SmlI     SphI
     StyI     TstI     XhoI     XmnI

 Enzymes that do not cut: 

    AatII     AccI     AclI     AcuI     AfeI   AflIII     AgeI     AleI
     AlfI     AloI     ApaI    ApaLI     AscI     AseI    AsiSI     BaeI
    BamHI    BanII     BbeI     BbsI    BbvCI     BcgI    BciVI     BclI
    BfrBI     BglI     BlpI Bme1580I    BmgBI     BmrI     BmtI     BplI
     BpmI   Bpu10I    BpuEI    BsaAI    BsaBI    BsaHI    BsaXI    BsiEI
  BsiHKAI    BsiWI     BsmI    BsmBI Bsp1286I    BspHI    BsrBI    BsrDI
    BsrFI    BsrGI   BssHII    BssSI   BstAPI    BstBI   BstEII    BstXI
  BstZ17I   Bsu36I    BtgZI     ClaI    CspCI     DraI   DraIII     DrdI
     EaeI     EagI     EarI  EcoICRI  Eco57MI    EcoNI EcoO109I    EcoRV
     FseI     FspI    FspAI    HaeII   HincII  HindIII     HpaI     KasI
     MfeI     MluI     MscI   MspA1I     NaeI     NarI   NgoMIV     NheI
     NotI     NruI     NsiI     PacI     PasI     PciI    PflMI     PfoI
     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI   PspOMI    PspXI
     PsrI     PstI     PvuI    PvuII    RsrII     SacI    SacII     SalI
    SanDI     SapI     SbfI     ScaI    SexAI     SfcI     SfiI     SfoI
     SgfI    SgrAI     SmaI    SnaBI     SpeI     SrfI     SspI     StuI
     SwaI    TaqII     TatI  Tth111I     XbaI     XcmI     XmaI     ZraI