Restriction map for
pFN21AE1446
(Linear) (Six-base) MAPSORT of: KIEE1446.seq Check: 3856 from: 1 to: 975
KIEE1446.orf
With 182 enzymes: SgfI *
July 15, 2010 18:39 ..
AhdI GACnn_n'nnGTC
Cuts at: 0 686 975
Size: 686 289
ApoI r'AATT_y
Cuts at: 0 958 975
Size: 958 17
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 912 945 975
Size: 912 33 30
Fragments arranged by size:
912 33 30
BamHI G'GATC_C
Cuts at: 0 384 975
Size: 384 591
BanI G'GyrC_C
Cuts at: 0 136 975
Size: 136 839
BanII G_rGCy'C
Cuts at: 0 413 509 975
Size: 413 96 466
Fragments arranged by size:
466 413 96
BbsI GAAGACnn'nnnn_
Cuts at: 0 676 975
Size: 676 299
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 31 975
Size: 31 944
BciVI GTATCCnnnnn_n'
Cuts at: 0 940 975
Size: 940 35
BlpI GC'TnA_GC
Cuts at: 0 210 975
Size: 210 765
Bme1580I G_kGCm'C
Cuts at: 0 139 975
Size: 139 836
BmrI ACTGGGnnnn_n'
Cuts at: 0 192 975
Size: 192 783
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 88 975
Size: 88 887
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 551 975
Size: 551 424
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 253 975
Size: 253 722
BsaI GGTCTCn'nnnn_
Cuts at: 0 59 975
Size: 59 916
BsaWI w'CCGG_w
Cuts at: 0 154 287 677 975
Size: 154 133 390 298
Fragments arranged by size:
390 298 154 133
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 61 975
Size: 61 914
BseYI C'CCAG_C
Cuts at: 0 132 975
Size: 132 843
BsiHKAI G_wGCw'C
Cuts at: 0 509 560 975
Size: 509 51 415
Fragments arranged by size:
509 415 51
BsmBI CGTCTCn'nnnn_
Cuts at: 0 235 975
Size: 235 740
Bsp1286I G_dGCh'C
Cuts at: 0 139 413 509 560 975
Size: 139 274 96 51 415
Fragments arranged by size:
415 274 139 96 51
BspEI T'CCGG_A
Cuts at: 0 287 677 975
Size: 287 390 298
Fragments arranged by size:
390 298 287
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 301 386 975
Size: 301 85 589
Fragments arranged by size:
589 301 85
BsrBI CCG'CTC
Cuts at: 0 30 975
Size: 30 945
BsrDI GCAATG_nn'
Cuts at: 0 438 975
Size: 438 537
BstBI TT'CG_AA
Cuts at: 0 956 975
Size: 956 19
BstXI CCAn_nnnn'nTGG
Cuts at: 0 499 886 975
Size: 499 387 89
Fragments arranged by size:
499 387 89
BstYI r'GATC_y
Cuts at: 0 384 422 975
Size: 384 38 553
Fragments arranged by size:
553 384 38
BtgI C'CryG_G
Cuts at: 0 332 755 975
Size: 332 423 220
Fragments arranged by size:
423 332 220
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 468 975
Size: 468 507
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 639 674 765 800 975
Size: 639 35 91 35 175
Fragments arranged by size:
639 175 91 35 35
DrdI GACnn_nn'nnGTC
Cuts at: 0 97 730 975
Size: 97 633 245
Fragments arranged by size:
633 245 97
EaeI y'GGCC_r
Cuts at: 0 83 489 975
Size: 83 406 486
Fragments arranged by size:
486 406 83
EarI CTCTTCn'nnn_
Cuts at: 0 821 831 975
Size: 821 10 144
Fragments arranged by size:
821 144 10
EcoICRI GAG'CTC
Cuts at: 0 507 975
Size: 507 468
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 551 975
Size: 551 424
EcoNI CCTnn'n_nnAGG
Cuts at: 0 16 777 975
Size: 16 761 198
Fragments arranged by size:
761 198 16
EcoO109I rG'GnC_Cy
Cuts at: 0 90 463 975
Size: 90 373 512
Fragments arranged by size:
512 373 90
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 867 975
Size: 867 108
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 672 704 975
Size: 672 32 271
Fragments arranged by size:
672 271 32
HincII GTy'rAC
Cuts at: 0 696 975
Size: 696 279
HindIII A'AGCT_T
Cuts at: 0 637 975
Size: 637 338
MscI TGG'CCA
Cuts at: 0 85 491 975
Size: 85 406 484
Fragments arranged by size:
484 406 85
MslI CAynn'nnrTG
Cuts at: 0 142 322 497 601 607 884 975
Size: 142 180 175 104 6 277 91
Fragments arranged by size:
277 180 175 142 104 91 6
MspA1I CmG'CkG
Cuts at: 0 214 224 258 703 905 975
Size: 214 10 34 445 202 70
Fragments arranged by size:
445 214 202 70 34 10
NcoI C'CATG_G
Cuts at: 0 755 975
Size: 755 220
NspI r_CATG'y
Cuts at: 0 199 975
Size: 199 776
PasI CC'CwG_GG
Cuts at: 0 468 975
Size: 468 507
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 787 819 975
Size: 787 32 156
Fragments arranged by size:
787 156 32
PpuMI rG'GwC_Cy
Cuts at: 0 90 463 975
Size: 90 373 512
Fragments arranged by size:
512 373 90
PstI C_TGCA'G
Cuts at: 0 20 975
Size: 20 955
PvuII CAG'CTG
Cuts at: 0 214 224 258 703 975
Size: 214 10 34 445 272
Fragments arranged by size:
445 272 214 34 10
SacI G_AGCT'C
Cuts at: 0 509 975
Size: 509 466
SanDI GG'GwC_CC
Cuts at: 0 463 975
Size: 463 512
ScaI AGT'ACT
Cuts at: 0 952 975
Size: 952 23
SfcI C'TryA_G
Cuts at: 0 16 897 975
Size: 16 881 78
Fragments arranged by size:
881 78 16
SmlI C'TyrA_G
Cuts at: 0 268 975
Size: 268 707
StyI C'CwwG_G
Cuts at: 0 86 620 755 975
Size: 86 534 135 220
Fragments arranged by size:
534 220 135 86
TatI w'GTAC_w
Cuts at: 0 950 975
Size: 950 25
Tth111I GACn'n_nGTC
Cuts at: 0 615 975
Size: 615 360
XmnI GAAnn'nnTTC
Cuts at: 0 673 975
Size: 673 302
Enzymes that do cut:
AhdI ApoI BaeI BamHI BanI BanII BbsI BcgI
BciVI BlpI Bme1580I BmrI BplI BpmI BpuEI BsaI
BsaWI BseRI BseYI BsiHKAI BsmBI Bsp1286I BspEI BspMI
BsrBI BsrDI BstBI BstXI BstYI BtgI BtgZI CspCI
DrdI EaeI EarI EcoICRI Eco57MI EcoNI EcoO109I FalI
Hin4I HincII HindIII MscI MslI MspA1I NcoI NspI
PasI PpiI PpuMI PstI PvuII SacI SanDI ScaI
SfcI SmlI StyI TatI Tth111I XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AleI AlfI AloI AlwNI ApaI ApaLI
AscI AseI AsiSI AvaI AvrII BbeI BbvCI BclI
BfrBI BglI BglII BmgBI BmtI Bpu10I BsaAI BsaBI
BsaHI BsaXI BsgI BsiEI BsiWI BsmI BspHI BsrFI
BsrGI BssHII BssSI BstAPI BstEII BstZ17I Bsu36I BtsI
ClaI DraI DraIII EagI EciI EcoRI EcoRV FseI
FspI FspAI HaeII HpaI KasI KpnI MfeI MluI
MmeI NaeI NarI NdeI NgoMIV NheI NotI NruI
NsiI PacI PciI PflMI PfoI PmeI PmlI PshAI
PsiI PspOMI PspXI PsrI PvuI RsrII SacII SalI
SapI SbfI SexAI SfiI SfoI SgfI SgrAI SmaI
SnaBI SpeI SphI SrfI SspI StuI SwaI TaqII
TstI XbaI XcmI XhoI XmaI ZraI