Restriction map for
pFN21AE1445
(Linear) (Six-base) MAPSORT of: KIEE1445.seq Check: 4996 from: 1 to: 879
KIEE1445.orf
With 182 enzymes: SgfI *
July 15, 2010 18:39 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 28 208 879
Size: 28 180 671
Fragments arranged by size:
671 180 28
AleI CACnn'nnGTG
Cuts at: 0 751 879
Size: 751 128
ApoI r'AATT_y
Cuts at: 0 296 404 879
Size: 296 108 475
Fragments arranged by size:
475 296 108
AvaI C'yCGr_G
Cuts at: 0 12 879
Size: 12 867
BanI G'GyrC_C
Cuts at: 0 29 196 879
Size: 29 167 683
Fragments arranged by size:
683 167 29
BanII G_rGCy'C
Cuts at: 0 7 20 879
Size: 7 13 859
Fragments arranged by size:
859 13 7
BbsI GAAGACnn'nnnn_
Cuts at: 0 342 879
Size: 342 537
BfrBI ATG'CAT
Cuts at: 0 385 879
Size: 385 494
BglI GCCn_nnn'nGGC
Cuts at: 0 189 879
Size: 189 690
BglII A'GATC_T
Cuts at: 0 237 879
Size: 237 642
Bme1580I G_kGCm'C
Cuts at: 0 32 199 879
Size: 32 167 680
Fragments arranged by size:
680 167 32
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 626 879
Size: 626 253
Bpu10I CC'TnA_GC
Cuts at: 0 355 879
Size: 355 524
BsaBI GATnn'nnATC
Cuts at: 0 472 879
Size: 472 407
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 141 711 733 879
Size: 141 570 22 146
Fragments arranged by size:
570 146 141 22
BsiHKAI G_wGCw'C
Cuts at: 0 7 879
Size: 7 872
Bsp1286I G_dGCh'C
Cuts at: 0 7 20 32 199 879
Size: 7 13 12 167 680
Fragments arranged by size:
680 167 13 12 7
BspHI T'CATG_A
Cuts at: 0 153 300 879
Size: 153 147 579
Fragments arranged by size:
579 153 147
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 28 208 218 810 879
Size: 28 180 10 592 69
Fragments arranged by size:
592 180 69 28 10
BstAPI GCAn_nnn'nTGC
Cuts at: 0 391 507 879
Size: 391 116 372
Fragments arranged by size:
391 372 116
BstYI r'GATC_y
Cuts at: 0 237 879
Size: 237 642
BtgI C'CryG_G
Cuts at: 0 360 879
Size: 360 519
BtsI GCAGTG_nn'
Cuts at: 0 178 879
Size: 178 701
DraIII CAC_nnn'GTG
Cuts at: 0 507 879
Size: 507 372
EcoICRI GAG'CTC
Cuts at: 0 5 879
Size: 5 874
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 626 879
Size: 626 253
FspI TGC'GCA
Cuts at: 0 204 879
Size: 204 675
HaeII r_GCGC'y
Cuts at: 0 777 879
Size: 777 102
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 568 600 879
Size: 568 32 279
Fragments arranged by size:
568 279 32
HincII GTy'rAC
Cuts at: 0 311 879
Size: 311 568
MfeI C'AATT_G
Cuts at: 0 806 879
Size: 806 73
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 435 533 879
Size: 435 98 346
Fragments arranged by size:
435 346 98
MslI CAynn'nnrTG
Cuts at: 0 326 380 751 879
Size: 326 54 371 128
Fragments arranged by size:
371 326 128 54
NcoI C'CATG_G
Cuts at: 0 360 879
Size: 360 519
NdeI CA'TA_TG
Cuts at: 0 387 879
Size: 387 492
NsiI A_TGCA'T
Cuts at: 0 387 879
Size: 387 492
NspI r_CATG'y
Cuts at: 0 488 879
Size: 488 391
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 275 307 879
Size: 275 32 572
Fragments arranged by size:
572 275 32
PshAI GACnn'nnGTC
Cuts at: 0 440 879
Size: 440 439
SacI G_AGCT'C
Cuts at: 0 7 879
Size: 7 872
StyI C'CwwG_G
Cuts at: 0 360 879
Size: 360 519
TatI w'GTAC_w
Cuts at: 0 107 879
Size: 107 772
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 236 268 879
Size: 236 32 611
Fragments arranged by size:
611 236 32
Enzymes that do cut:
AarI AleI ApoI AvaI BanI BanII BbsI BfrBI
BglI BglII Bme1580I BpmI Bpu10I BsaBI BseRI BsiHKAI
Bsp1286I BspHI BspMI BstAPI BstYI BtgI BtsI DraIII
EcoICRI Eco57MI FspI HaeII Hin4I HincII MfeI MmeI
MslI NcoI NdeI NsiI NspI PpiI PshAI SacI
StyI TatI TstI
Enzymes that do not cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AlfI AloI AlwNI ApaI ApaLI AscI
AseI AsiSI AvrII BaeI BamHI BbeI BbvCI BcgI
BciVI BclI BlpI BmgBI BmrI BmtI BplI BpuEI
BsaI BsaAI BsaHI BsaWI BsaXI BseYI BsgI BsiEI
BsiWI BsmI BsmBI BspEI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstBI BstEII BstXI BstZ17I Bsu36I BtgZI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
EcoNI EcoO109I EcoRI EcoRV FalI FseI FspAI HindIII
HpaI KasI KpnI MluI MscI MspA1I NaeI NarI
NgoMIV NheI NotI NruI PacI PasI PciI PflMI
PfoI PmeI PmlI PpuMI PsiI PspOMI PspXI PsrI
PstI PvuI PvuII RsrII SacII SalI SanDI SapI
SbfI ScaI SexAI SfcI SfiI SfoI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI
SwaI TaqII Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI