Restriction map for
pFN21AE1443
(Linear) (Six-base) MAPSORT of: KIEE1443.seq Check: 3641 from: 1 to: 1398
KIEE1443.orf
With 182 enzymes: SgfI *
July 15, 2010 18:39 ..
AccI GT'mk_AC
Cuts at: 0 1064 1398
Size: 1064 334
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 763 1125 1398
Size: 763 362 273
Fragments arranged by size:
763 362 273
AfeI AGC'GCT
Cuts at: 0 133 1398
Size: 133 1265
AvaI C'yCGr_G
Cuts at: 0 815 930 961 1131 1194 1398
Size: 815 115 31 170 63 204
Fragments arranged by size:
815 204 170 115 63 31
BamHI G'GATC_C
Cuts at: 0 373 876 912 1398
Size: 373 503 36 486
Fragments arranged by size:
503 486 373 36
BanI G'GyrC_C
Cuts at: 0 290 1135 1198 1278 1398
Size: 290 845 63 80 120
Fragments arranged by size:
845 290 120 80 63
BanII G_rGCy'C
Cuts at: 0 58 519 962 1023 1398
Size: 58 461 443 61 375
Fragments arranged by size:
461 443 375 61 58
BbeI G_GCGC'C
Cuts at: 0 1139 1398
Size: 1139 259
BbsI GAAGACnn'nnnn_
Cuts at: 0 1106 1398
Size: 1106 292
BbvCI CC'TCA_GC
Cuts at: 0 845 1398
Size: 845 553
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 682 716 1007 1041 1398
Size: 682 34 291 34 357
Fragments arranged by size:
682 357 291 34 34
BglI GCCn_nnn'nGGC
Cuts at: 0 53 1167 1297 1398
Size: 53 1114 130 101
Fragments arranged by size:
1114 130 101 53
BglII A'GATC_T
Cuts at: 0 585 758 1398
Size: 585 173 640
Fragments arranged by size:
640 585 173
BlpI GC'TnA_GC
Cuts at: 0 1287 1398
Size: 1287 111
BmgBI CAC'GTC
Cuts at: 0 1104 1398
Size: 1104 294
BmtI G_CTAG'C
Cuts at: 0 1150 1398
Size: 1150 248
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 63 973 1398
Size: 63 910 425
Fragments arranged by size:
910 425 63
Bpu10I CC'TnA_GC
Cuts at: 0 845 1183 1398
Size: 845 338 215
Fragments arranged by size:
845 338 215
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 289 310 412 505 1398
Size: 289 21 102 93 893
Fragments arranged by size:
893 289 102 93 21
BsaI GGTCTCn'nnnn_
Cuts at: 0 870 1222 1398
Size: 870 352 176
Fragments arranged by size:
870 352 176
BsaAI yAC'GTr
Cuts at: 0 1073 1398
Size: 1073 325
BsaHI Gr'CG_yC
Cuts at: 0 824 1136 1398
Size: 824 312 262
Fragments arranged by size:
824 312 262
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 166 862 970 1105 1398
Size: 166 696 108 135 293
Fragments arranged by size:
696 293 166 135 108
BseYI C'CCAG_C
Cuts at: 0 395 651 1327 1332 1398
Size: 395 256 676 5 66
Fragments arranged by size:
676 395 256 66 5
BsiEI CG_ry'CG
Cuts at: 0 202 1398
Size: 202 1196
BsiHKAI G_wGCw'C
Cuts at: 0 1078 1189 1208 1398
Size: 1078 111 19 190
Fragments arranged by size:
1078 190 111 19
BsmBI CGTCTCn'nnnn_
Cuts at: 0 831 1398
Size: 831 567
Bsp1286I G_dGCh'C
Cuts at: 0 58 519 962 1023 1078 1189 1208 1398
Size: 58 461 443 61 55 111 19 190
Fragments arranged by size:
461 443 190 111 61 58 55 19
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 442 1398
Size: 442 956
BsrFI r'CCGG_y
Cuts at: 0 34 169 287 1372 1398
Size: 34 135 118 1085 26
Fragments arranged by size:
1085 135 118 34 26
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1375 1398
Size: 1375 23
BstXI CCAn_nnnn'nTGG
Cuts at: 0 663 729 789 810 1398
Size: 663 66 60 21 588
Fragments arranged by size:
663 588 66 60 21
BstYI r'GATC_y
Cuts at: 0 373 585 758 876 912 1398
Size: 373 212 173 118 36 486
Fragments arranged by size:
486 373 212 173 118 36
BtgI C'CryG_G
Cuts at: 0 797 1398
Size: 797 601
BtsI GCAGTG_nn'
Cuts at: 0 930 1206 1248 1398
Size: 930 276 42 150
Fragments arranged by size:
930 276 150 42
ClaI AT'CG_AT
Cuts at: 0 692 1398
Size: 692 706
EaeI y'GGCC_r
Cuts at: 0 32 199 209 800 1398
Size: 32 167 10 591 598
Fragments arranged by size:
598 591 167 32 10
EagI C'GGCC_G
Cuts at: 0 199 1398
Size: 199 1199
EarI CTCTTCn'nnn_
Cuts at: 0 743 1345 1398
Size: 743 602 53
Fragments arranged by size:
743 602 53
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 129 1398
Size: 129 1269
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 63 763 973 1125 1398
Size: 63 700 210 152 273
Fragments arranged by size:
700 273 210 152 63
EcoNI CCTnn'n_nnAGG
Cuts at: 0 211 1084 1398
Size: 211 873 314
Fragments arranged by size:
873 314 211
EcoO109I rG'GnC_Cy
Cuts at: 0 357 1398
Size: 357 1041
HaeII r_GCGC'y
Cuts at: 0 135 170 1139 1398
Size: 135 35 969 259
Fragments arranged by size:
969 259 135 35
KasI G'GCGC_C
Cuts at: 0 1135 1398
Size: 1135 263
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 344 1398
Size: 344 1054
MscI TGG'CCA
Cuts at: 0 211 802 1398
Size: 211 591 596
Fragments arranged by size:
596 591 211
MslI CAynn'nnrTG
Cuts at: 0 338 1398
Size: 338 1060
MspA1I CmG'CkG
Cuts at: 0 19 105 399 644 951 1056 1398
Size: 19 86 294 245 307 105 342
Fragments arranged by size:
342 307 294 245 105 86 19
NaeI GCC'GGC
Cuts at: 0 36 171 289 1398
Size: 36 135 118 1109
Fragments arranged by size:
1109 135 118 36
NarI GG'CG_CC
Cuts at: 0 1136 1398
Size: 1136 262
NcoI C'CATG_G
Cuts at: 0 797 1398
Size: 797 601
NgoMIV G'CCGG_C
Cuts at: 0 34 169 287 1398
Size: 34 135 118 1111
Fragments arranged by size:
1111 135 118 34
NheI G'CTAG_C
Cuts at: 0 1146 1398
Size: 1146 252
NotI GC'GGCC_GC
Cuts at: 0 199 1398
Size: 199 1199
NspI r_CATG'y
Cuts at: 0 997 1398
Size: 997 401
PfoI T'CCnGG_A
Cuts at: 0 539 1398
Size: 539 859
PmlI CAC'GTG
Cuts at: 0 1073 1398
Size: 1073 325
PpuMI rG'GwC_Cy
Cuts at: 0 357 1398
Size: 357 1041
PstI C_TGCA'G
Cuts at: 0 18 24 632 925 1055 1124 1398
Size: 18 6 608 293 130 69 274
Fragments arranged by size:
608 293 274 130 69 18 6
PvuII CAG'CTG
Cuts at: 0 19 105 399 644 951 1398
Size: 19 86 294 245 307 447
Fragments arranged by size:
447 307 294 245 86 19
SapI GCTCTTCn'nnn_
Cuts at: 0 743 1398
Size: 743 655
SfcI C'TryA_G
Cuts at: 0 14 20 628 921 1051 1120 1398
Size: 14 6 608 293 130 69 278
Fragments arranged by size:
608 293 278 130 69 14 6
SfoI GGC'GCC
Cuts at: 0 1137 1398
Size: 1137 261
SmaI CCC'GGG
Cuts at: 0 817 963 1133 1196 1398
Size: 817 146 170 63 202
Fragments arranged by size:
817 202 170 146 63
SmlI C'TyrA_G
Cuts at: 0 304 325 427 520 1398
Size: 304 21 102 93 878
Fragments arranged by size:
878 304 102 93 21
SphI G_CATG'C
Cuts at: 0 997 1398
Size: 997 401
SrfI GCCC'GGGC
Cuts at: 0 817 1398
Size: 817 581
StuI AGG'CCT
Cuts at: 0 381 1181 1398
Size: 381 800 217
Fragments arranged by size:
800 381 217
StyI C'CwwG_G
Cuts at: 0 212 750 797 1398
Size: 212 538 47 601
Fragments arranged by size:
601 538 212 47
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 614 1341 1398
Size: 614 727 57
Fragments arranged by size:
727 614 57
TatI w'GTAC_w
Cuts at: 0 74 1398
Size: 74 1324
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1025 1057 1398
Size: 1025 32 341
Fragments arranged by size:
1025 341 32
XmaI C'CCGG_G
Cuts at: 0 815 961 1131 1194 1398
Size: 815 146 170 63 204
Fragments arranged by size:
815 204 170 146 63
Enzymes that do cut:
AccI AcuI AfeI AvaI BamHI BanI BanII BbeI
BbsI BbvCI BcgI BglI BglII BlpI BmgBI BmtI
BpmI Bpu10I BpuEI BsaI BsaAI BsaHI BseRI BseYI
BsiEI BsiHKAI BsmBI Bsp1286I BspMI BsrFI BstAPI BstXI
BstYI BtgI BtsI ClaI EaeI EagI EarI EciI
Eco57MI EcoNI EcoO109I HaeII KasI MmeI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NheI NotI NspI
PfoI PmlI PpuMI PstI PvuII SapI SfcI SfoI
SmaI SmlI SphI SrfI StuI StyI TaqII TatI
TstI XmaI
Enzymes that do not cut:
AarI AatII Acc65I AclI AflII AflIII AgeI AhdI
AleI AlfI AloI AlwNI ApaI ApaLI ApoI AscI
AseI AsiSI AvrII BaeI BciVI BclI BfrBI Bme1580I
BmrI BplI BsaBI BsaWI BsaXI BsgI BsiWI BsmI
BspEI BspHI BsrBI BsrDI BsrGI BssHII BssSI BstBI
BstEII BstZ17I Bsu36I BtgZI CspCI DraI DraIII DrdI
EcoICRI EcoRI EcoRV FalI FseI FspI FspAI Hin4I
HincII HindIII HpaI KpnI MfeI MluI NdeI NruI
NsiI PacI PasI PciI PflMI PmeI PpiI PshAI
PsiI PspOMI PspXI PsrI PvuI RsrII SacI SacII
SalI SanDI SbfI ScaI SexAI SfiI SgfI SgrAI
SnaBI SpeI SspI SwaI Tth111I XbaI XcmI XhoI
XmnI ZraI