Restriction map for
pFN21AE1442
(Linear) (Six-base) MAPSORT of: KIEE1442.seq Check: 7774 from: 1 to: 765
KIEE1442.orf
With 182 enzymes: SgfI *
July 15, 2010 18:39 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 157 765
Size: 157 608
AatII G_ACGT'C
Cuts at: 0 586 765
Size: 586 179
AccI GT'mk_AC
Cuts at: 0 399 586 765
Size: 399 187 179
Fragments arranged by size:
399 187 179
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 216 248 399 431 765
Size: 216 32 151 32 334
Fragments arranged by size:
334 216 151 32 32
AlwNI CAG_nnn'CTG
Cuts at: 0 140 765
Size: 140 625
BamHI G'GATC_C
Cuts at: 0 75 765
Size: 75 690
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 576 610 765
Size: 576 34 155
Fragments arranged by size:
576 155 34
BmgBI CAC'GTC
Cuts at: 0 465 765
Size: 465 300
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 252 765
Size: 252 513
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 144 251 467 765
Size: 144 107 216 298
Fragments arranged by size:
298 216 144 107
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 689 765
Size: 689 76
BsaI GGTCTCn'nnnn_
Cuts at: 0 681 765
Size: 681 84
BsaHI Gr'CG_yC
Cuts at: 0 583 765
Size: 583 182
BseYI C'CCAG_C
Cuts at: 0 93 765
Size: 93 672
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 246 765
Size: 246 519
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 157 765
Size: 157 608
BsrDI GCAATG_nn'
Cuts at: 0 67 252 765
Size: 67 185 513
Fragments arranged by size:
513 185 67
BstYI r'GATC_y
Cuts at: 0 75 765
Size: 75 690
BtsI GCAGTG_nn'
Cuts at: 0 205 276 765
Size: 205 71 489
Fragments arranged by size:
489 205 71
EarI CTCTTCn'nnn_
Cuts at: 0 604 765
Size: 604 161
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 144 251 467 765
Size: 144 107 216 298
Fragments arranged by size:
298 216 144 107
EcoO109I rG'GnC_Cy
Cuts at: 0 179 765
Size: 179 586
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 120 152 415 447 765
Size: 120 32 263 32 318
Fragments arranged by size:
318 263 120 32 32
HincII GTy'rAC
Cuts at: 0 400 587 765
Size: 400 187 178
Fragments arranged by size:
400 187 178
MspA1I CmG'CkG
Cuts at: 0 11 97 765
Size: 11 86 668
Fragments arranged by size:
668 86 11
NdeI CA'TA_TG
Cuts at: 0 190 765
Size: 190 575
NspI r_CATG'y
Cuts at: 0 303 765
Size: 303 462
PfoI T'CCnGG_A
Cuts at: 0 498 765
Size: 498 267
PpuMI rG'GwC_Cy
Cuts at: 0 179 765
Size: 179 586
PvuII CAG'CTG
Cuts at: 0 11 97 765
Size: 11 86 668
Fragments arranged by size:
668 86 11
SalI G'TCGA_C
Cuts at: 0 398 585 765
Size: 398 187 180
Fragments arranged by size:
398 187 180
SapI GCTCTTCn'nnn_
Cuts at: 0 604 765
Size: 604 161
SfcI C'TryA_G
Cuts at: 0 720 765
Size: 720 45
SmlI C'TyrA_G
Cuts at: 0 704 765
Size: 704 61
TatI w'GTAC_w
Cuts at: 0 693 765
Size: 693 72
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 419 451 765
Size: 419 32 314
Fragments arranged by size:
419 314 32
Tth111I GACn'n_nGTC
Cuts at: 0 466 765
Size: 466 299
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 36 765
Size: 36 729
ZraI GAC'GTC
Cuts at: 0 584 765
Size: 584 181
Enzymes that do cut:
AarI AatII AccI AloI AlwNI BamHI BcgI BmgBI
BplI BpmI BpuEI BsaI BsaHI BseYI BsgI BspMI
BsrDI BstYI BtsI EarI Eco57MI EcoO109I Hin4I HincII
MspA1I NdeI NspI PfoI PpuMI PvuII SalI SapI
SfcI SmlI TatI TstI Tth111I XcmI ZraI
Enzymes that do not cut:
Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI
AleI AlfI ApaI ApaLI ApoI AscI AseI AsiSI
AvaI AvrII BaeI BanI BanII BbeI BbsI BbvCI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmrI
BmtI Bpu10I BsaAI BsaBI BsaWI BsaXI BseRI BsiEI
BsiHKAI BsiWI BsmI BsmBI Bsp1286I BspEI BspHI BsrBI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstZ17I Bsu36I BtgI BtgZI ClaI CspCI DraI DraIII
DrdI EaeI EagI EciI EcoICRI EcoNI EcoRI EcoRV
FalI FseI FspI FspAI HaeII HindIII HpaI KasI
KpnI MfeI MluI MmeI MscI MslI NaeI NarI
NcoI NgoMIV NheI NotI NruI NsiI PacI PasI
PciI PflMI PmeI PmlI PpiI PshAI PsiI PspOMI
PspXI PsrI PstI PvuI RsrII SacI SacII SanDI
SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SmaI
SnaBI SpeI SphI SrfI SspI StuI StyI SwaI
TaqII XbaI XhoI XmaI XmnI