Restriction map for
pFN21AE1436
(Linear) (Six-base) MAPSORT of: KIEE1436.seq Check: 9046 from: 1 to: 759
KIEE1436.orf
With 182 enzymes: SgfI *
July 15, 2010 18:38 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 490 759
Size: 490 269
AgeI A'CCGG_T
Cuts at: 0 625 759
Size: 625 134
ApaI G_GGCC'C
Cuts at: 0 101 280 289 309 759
Size: 101 179 9 20 450
Fragments arranged by size:
450 179 101 20 9
ApaLI G'TGCA_C
Cuts at: 0 569 759
Size: 569 190
AvaI C'yCGr_G
Cuts at: 0 120 527 759
Size: 120 407 232
Fragments arranged by size:
407 232 120
BanI G'GyrC_C
Cuts at: 0 489 709 759
Size: 489 220 50
Fragments arranged by size:
489 220 50
BanII G_rGCy'C
Cuts at: 0 101 280 289 309 318 464 759
Size: 101 179 9 20 9 146 295
Fragments arranged by size:
295 179 146 101 20 9 9
BbsI GAAGACnn'nnnn_
Cuts at: 0 657 759
Size: 657 102
BciVI GTATCCnnnnn_n'
Cuts at: 0 136 759
Size: 136 623
BclI T'GATC_A
Cuts at: 0 434 759
Size: 434 325
BglI GCCn_nnn'nGGC
Cuts at: 0 149 275 284 759
Size: 149 126 9 475
Fragments arranged by size:
475 149 126 9
BlpI GC'TnA_GC
Cuts at: 0 85 759
Size: 85 674
Bme1580I G_kGCm'C
Cuts at: 0 101 280 289 309 494 573 759
Size: 101 179 9 20 185 79 186
Fragments arranged by size:
186 185 179 101 79 20 9
BmrI ACTGGGnnnn_n'
Cuts at: 0 25 706 759
Size: 25 681 53
Fragments arranged by size:
681 53 25
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 224 332 667 759
Size: 224 108 335 92
Fragments arranged by size:
335 224 108 92
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 625 759
Size: 625 134
BsaI GGTCTCn'nnnn_
Cuts at: 0 200 227 502 759
Size: 200 27 275 257
Fragments arranged by size:
275 257 200 27
BsaAI yAC'GTr
Cuts at: 0 432 759
Size: 432 327
BsaBI GATnn'nnATC
Cuts at: 0 10 759
Size: 10 749
BsaWI w'CCGG_w
Cuts at: 0 625 759
Size: 625 134
BseYI C'CCAG_C
Cuts at: 0 80 102 759
Size: 80 22 657
Fragments arranged by size:
657 80 22
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 75 759
Size: 75 684
BsiHKAI G_wGCw'C
Cuts at: 0 573 759
Size: 573 186
Bsp1286I G_dGCh'C
Cuts at: 0 101 280 289 309 318 464 494 573
Size: 101 179 9 20 9 146 30 79
Cuts at: 573 759
Size: 186
Fragments arranged by size:
186 179 146 101 79 30 20 9
9
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 490 759
Size: 490 269
BsrFI r'CCGG_y
Cuts at: 0 625 759
Size: 625 134
BstEII G'GTnAC_C
Cuts at: 0 582 618 759
Size: 582 36 141
Fragments arranged by size:
582 141 36
BstYI r'GATC_y
Cuts at: 0 11 759
Size: 11 748
BtgI C'CryG_G
Cuts at: 0 16 210 281 759
Size: 16 194 71 478
Fragments arranged by size:
478 194 71 16
BtsI GCAGTG_nn'
Cuts at: 0 474 759
Size: 474 285
ClaI AT'CG_AT
Cuts at: 0 65 759
Size: 65 694
EaeI y'GGCC_r
Cuts at: 0 150 558 759
Size: 150 408 201
Fragments arranged by size:
408 201 150
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 224 332 667 759
Size: 224 108 335 92
Fragments arranged by size:
335 224 108 92
EcoO109I rG'GnC_Cy
Cuts at: 0 98 161 276 277 286 305 306 322
Size: 98 63 115 1 9 19 1 16
Cuts at: 322 759
Size: 437
Fragments arranged by size:
437 115 98 63 19 16 9 1
1
EcoRV GAT'ATC
Cuts at: 0 69 759
Size: 69 690
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 673 705 759
Size: 673 32 54
Fragments arranged by size:
673 54 32
MscI TGG'CCA
Cuts at: 0 152 759
Size: 152 607
MslI CAynn'nnrTG
Cuts at: 0 442 759
Size: 442 317
MspA1I CmG'CkG
Cuts at: 0 89 759
Size: 89 670
NcoI C'CATG_G
Cuts at: 0 16 210 281 759
Size: 16 194 71 478
Fragments arranged by size:
478 194 71 16
PasI CC'CwG_GG
Cuts at: 0 130 166 238 301 759
Size: 130 36 72 63 458
Fragments arranged by size:
458 130 72 63 36
PfoI T'CCnGG_A
Cuts at: 0 255 759
Size: 255 504
PmlI CAC'GTG
Cuts at: 0 432 759
Size: 432 327
PpuMI rG'GwC_Cy
Cuts at: 0 161 759
Size: 161 598
PspOMI G'GGCC_C
Cuts at: 0 97 276 285 305 759
Size: 97 179 9 20 454
Fragments arranged by size:
454 179 97 20 9
PstI C_TGCA'G
Cuts at: 0 674 759
Size: 674 85
PvuII CAG'CTG
Cuts at: 0 89 759
Size: 89 670
SanDI GG'GwC_CC
Cuts at: 0 161 759
Size: 161 598
SbfI CC_TGCA'GG
Cuts at: 0 674 759
Size: 674 85
ScaI AGT'ACT
Cuts at: 0 35 759
Size: 35 724
SfcI C'TryA_G
Cuts at: 0 670 759
Size: 670 89
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 149 275 284 759
Size: 149 126 9 475
Fragments arranged by size:
475 149 126 9
SgrAI Cr'CCGG_yG
Cuts at: 0 625 759
Size: 625 134
SmaI CCC'GGG
Cuts at: 0 122 759
Size: 122 637
SmlI C'TyrA_G
Cuts at: 0 527 640 759
Size: 527 113 119
Fragments arranged by size:
527 119 113
StyI C'CwwG_G
Cuts at: 0 16 210 272 281 759
Size: 16 194 62 9 478
Fragments arranged by size:
478 194 62 16 9
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 370 759
Size: 370 389
TatI w'GTAC_w
Cuts at: 0 33 759
Size: 33 726
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 279 630 759
Size: 279 351 129
Fragments arranged by size:
351 279 129
XhoI C'TCGA_G
Cuts at: 0 527 759
Size: 527 232
XmaI C'CCGG_G
Cuts at: 0 120 759
Size: 120 639
XmnI GAAnn'nnTTC
Cuts at: 0 664 759
Size: 664 95
Enzymes that do cut:
AarI AgeI ApaI ApaLI AvaI BanI BanII BbsI
BciVI BclI BglI BlpI Bme1580I BmrI BpmI BpuEI
BsaI BsaAI BsaBI BsaWI BseYI BsgI BsiHKAI Bsp1286I
BspMI BsrFI BstEII BstYI BtgI BtsI ClaI EaeI
Eco57MI EcoO109I EcoRV Hin4I MscI MslI MspA1I NcoI
PasI PfoI PmlI PpuMI PspOMI PstI PvuII SanDI
SbfI ScaI SfcI SfiI SgrAI SmaI SmlI StyI
TaqII TatI XcmI XhoI XmaI XmnI
Enzymes that do not cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AhdI AleI AlfI AloI AlwNI ApoI AscI AseI
AsiSI AvrII BaeI BamHI BbeI BbvCI BcgI BfrBI
BglII BmgBI BmtI BplI Bpu10I BsaHI BsaXI BseRI
BsiEI BsiWI BsmI BsmBI BspEI BspHI BsrBI BsrDI
BsrGI BssHII BssSI BstAPI BstBI BstXI BstZ17I Bsu36I
BtgZI CspCI DraI DraIII DrdI EagI EarI EciI
EcoICRI EcoNI EcoRI FalI FseI FspI FspAI HaeII
HincII HindIII HpaI KasI KpnI MfeI MluI MmeI
NaeI NarI NdeI NgoMIV NheI NotI NruI NsiI
NspI PacI PciI PflMI PmeI PpiI PshAI PsiI
PspXI PsrI PvuI RsrII SacI SacII SalI SapI
SexAI SfoI SgfI SnaBI SpeI SphI SrfI SspI
StuI SwaI TstI Tth111I XbaI ZraI