(Linear) (Six-base) MAPSORT of: KIEE1435.seq  Check: 1818  from: 1  to: 831



KIEE1435.orf

 With 182 enzymes: SgfI * 

                        July 15, 2010 18:38  ..

AflIII A'CryG_T

Cuts at:      0     274     831 
   Size:        274     557
 
AlwNI CAG_nnn'CTG

Cuts at:      0     601     831 
   Size:        601     230
 
ApoI r'AATT_y

Cuts at:      0      42     159     621     691     736     831 
   Size:         42     117     462      70      45      95

  Fragments arranged by size: 

                 462     117      95      70      45      42
 
AseI AT'TA_AT

Cuts at:      0     149     831 
   Size:        149     682
 
AvaI C'yCGr_G

Cuts at:      0     710     831 
   Size:        710     121
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:      0     188     221     831 
   Size:        188      33     610

  Fragments arranged by size: 

                 610     188      33
 
BfrBI ATG'CAT

Cuts at:      0     586     831 
   Size:        586     245
 
BsaAI yAC'GTr

Cuts at:      0     277     831 
   Size:        277     554
 
Bsu36I CC'TnA_GG

Cuts at:      0     220     831 
   Size:        220     611
 
BtgI C'CryG_G

Cuts at:      0     360     489     831 
   Size:        360     129     342

  Fragments arranged by size: 

                 360     342     129
 
DrdI GACnn_nn'nnGTC

Cuts at:      0     189     831 
   Size:        189     642
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0      16      48     831 
   Size:         16      32     783

  Fragments arranged by size: 

                 783      32      16
 
HincII GTy'rAC

Cuts at:      0     402     717     831 
   Size:        402     315     114

  Fragments arranged by size: 

                 402     315     114
 
HindIII A'AGCT_T

Cuts at:      0     685     831 
   Size:        685     146
 
HpaI GTT'AAC

Cuts at:      0     717     831 
   Size:        717     114
 
MslI CAynn'nnrTG

Cuts at:      0     178     831 
   Size:        178     653
 
NcoI C'CATG_G

Cuts at:      0     360     831 
   Size:        360     471
 
NsiI A_TGCA'T

Cuts at:      0     588     831 
   Size:        588     243
 
PmlI CAC'GTG

Cuts at:      0     277     831 
   Size:        277     554
 
PsiI TTA'TAA

Cuts at:      0     110     831 
   Size:        110     721
 
SexAI A'CCwGG_T

Cuts at:      0     600     831 
   Size:        600     231
 
SmaI CCC'GGG

Cuts at:      0     712     831 
   Size:        712     119
 
StuI AGG'CCT

Cuts at:      0     484     831 
   Size:        484     347
 
StyI C'CwwG_G

Cuts at:      0     360     831 
   Size:        360     471
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     179     831 
   Size:        179     652
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0      42     831 
   Size:         42     789
 
XmaI C'CCGG_G

Cuts at:      0     710     831 
   Size:        710     121
 

 Enzymes that do cut:

   AflIII    AlwNI     ApoI     AseI     AvaI     BaeI    BfrBI    BsaAI
   Bsu36I     BtgI     DrdI    Hin4I   HincII  HindIII     HpaI     MslI
     NcoI     NsiI     PmlI     PsiI    SexAI     SmaI     StuI     StyI
    TaqII     XcmI     XmaI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
     AgeI     AhdI     AleI     AlfI     AloI     ApaI    ApaLI     AscI
    AsiSI    AvrII    BamHI     BanI    BanII     BbeI     BbsI    BbvCI
     BcgI    BciVI     BclI     BglI    BglII     BlpI Bme1580I    BmgBI
     BmrI     BmtI     BplI     BpmI   Bpu10I    BpuEI     BsaI    BsaBI
    BsaHI    BsaWI    BsaXI    BseRI    BseYI     BsgI    BsiEI  BsiHKAI
    BsiWI     BsmI    BsmBI Bsp1286I    BspEI    BspHI    BspMI    BsrBI
    BsrDI    BsrFI    BsrGI   BssHII    BssSI   BstAPI    BstBI   BstEII
    BstXI    BstYI  BstZ17I    BtgZI     BtsI     ClaI    CspCI     DraI
   DraIII     EaeI     EagI     EarI     EciI  EcoICRI  Eco57MI    EcoNI
 EcoO109I    EcoRI    EcoRV     FalI     FseI     FspI    FspAI    HaeII
     KasI     KpnI     MfeI     MluI     MmeI     MscI   MspA1I     NaeI
     NarI     NdeI   NgoMIV     NheI     NotI     NruI     NspI     PacI
     PasI     PciI    PflMI     PfoI     PmeI     PpiI    PpuMI    PshAI
   PspOMI    PspXI     PsrI     PstI     PvuI    PvuII    RsrII     SacI
    SacII     SalI    SanDI     SapI     SbfI     ScaI     SfcI     SfiI
     SfoI     SgfI    SgrAI     SmlI    SnaBI     SpeI     SphI     SrfI
     SspI     SwaI     TatI     TstI  Tth111I     XbaI     XhoI     XmnI
     ZraI