Restriction map for
pFN21AE1433
(Linear) (Six-base) MAPSORT of: KIEE1433.seq Check: 1386 from: 1 to: 1497
KIEE1433.orf
With 182 enzymes: SgfI *
July 15, 2010 18:38 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 25 1497
Size: 25 1472
ApaI G_GGCC'C
Cuts at: 0 954 1497
Size: 954 543
AvaI C'yCGr_G
Cuts at: 0 806 1331 1497
Size: 806 525 166
Fragments arranged by size:
806 525 166
BanI G'GyrC_C
Cuts at: 0 730 1126 1497
Size: 730 396 371
Fragments arranged by size:
730 396 371
BanII G_rGCy'C
Cuts at: 0 817 954 1241 1357 1497
Size: 817 137 287 116 140
Fragments arranged by size:
817 287 140 137 116
BbsI GAAGACnn'nnnn_
Cuts at: 0 493 1497
Size: 493 1004
BbvCI CC'TCA_GC
Cuts at: 0 743 1497
Size: 743 754
BciVI GTATCCnnnnn_n'
Cuts at: 0 532 1497
Size: 532 965
BglI GCCn_nnn'nGGC
Cuts at: 0 1306 1372 1497
Size: 1306 66 125
Fragments arranged by size:
1306 125 66
Bme1580I G_kGCm'C
Cuts at: 0 733 954 1129 1497
Size: 733 221 175 368
Fragments arranged by size:
733 368 221 175
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1260 1497
Size: 1260 237
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 462 1040 1497
Size: 462 578 457
Fragments arranged by size:
578 462 457
Bpu10I CC'TnA_GC
Cuts at: 0 283 743 820 1497
Size: 283 460 77 677
Fragments arranged by size:
677 460 283 77
BsaI GGTCTCn'nnnn_
Cuts at: 0 1226 1387 1497
Size: 1226 161 110
Fragments arranged by size:
1226 161 110
BsaHI Gr'CG_yC
Cuts at: 0 316 1497
Size: 316 1181
BseYI C'CCAG_C
Cuts at: 0 947 1031 1055 1497
Size: 947 84 24 442
Fragments arranged by size:
947 442 84 24
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 13 769 784 1481 1497
Size: 13 756 15 697 16
Fragments arranged by size:
756 697 16 15 13
BsiHKAI G_wGCw'C
Cuts at: 0 1017 1165 1210 1241 1497
Size: 1017 148 45 31 256
Fragments arranged by size:
1017 256 148 45 31
Bsp1286I G_dGCh'C
Cuts at: 0 733 817 954 1017 1129 1165 1210 1241
Size: 733 84 137 63 112 36 45 31
Cuts at: 1241 1357 1497
Size: 116 140
Fragments arranged by size:
733 140 137 116 112 84 63 45
36 31
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 35 499 1497
Size: 35 464 998
Fragments arranged by size:
998 464 35
BsrBI CCG'CTC
Cuts at: 0 1446 1497
Size: 1446 51
BsrFI r'CCGG_y
Cuts at: 0 154 1497
Size: 154 1343
BssHII G'CGCG_C
Cuts at: 0 76 1497
Size: 76 1421
BstAPI GCAn_nnn'nTGC
Cuts at: 0 36 73 657 1497
Size: 36 37 584 840
Fragments arranged by size:
840 584 37 36
BstBI TT'CG_AA
Cuts at: 0 783 1497
Size: 783 714
BstEII G'GTnAC_C
Cuts at: 0 226 1497
Size: 226 1271
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1110 1497
Size: 1110 387
BstYI r'GATC_y
Cuts at: 0 386 1497
Size: 386 1111
Bsu36I CC'TnA_GG
Cuts at: 0 1399 1497
Size: 1399 98
BtgI C'CryG_G
Cuts at: 0 596 1184 1251 1295 1497
Size: 596 588 67 44 202
Fragments arranged by size:
596 588 202 67 44
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 229 1497
Size: 229 1268
BtsI GCAGTG_nn'
Cuts at: 0 255 744 1497
Size: 255 489 753
Fragments arranged by size:
753 489 255
DraIII CAC_nnn'GTG
Cuts at: 0 903 916 1497
Size: 903 13 581
Fragments arranged by size:
903 581 13
EaeI y'GGCC_r
Cuts at: 0 269 593 861 905 918 971 1298 1497
Size: 269 324 268 44 13 53 327 199
Fragments arranged by size:
327 324 269 268 199 53 44 13
EcoICRI GAG'CTC
Cuts at: 0 1239 1497
Size: 1239 258
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 25 462 1040 1497
Size: 25 437 578 457
Fragments arranged by size:
578 457 437 25
EcoNI CCTnn'n_nnAGG
Cuts at: 0 286 1497
Size: 286 1211
EcoO109I rG'GnC_Cy
Cuts at: 0 951 1169 1335 1497
Size: 951 218 166 162
Fragments arranged by size:
951 218 166 162
HaeII r_GCGC'y
Cuts at: 0 155 1413 1497
Size: 155 1258 84
Fragments arranged by size:
1258 155 84
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 16 48 1497
Size: 16 32 1449
Fragments arranged by size:
1449 32 16
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1403 1497
Size: 1403 94
MscI TGG'CCA
Cuts at: 0 271 863 907 920 973 1300 1497
Size: 271 592 44 13 53 327 197
Fragments arranged by size:
592 327 271 197 53 44 13
MslI CAynn'nnrTG
Cuts at: 0 248 875 878 1497
Size: 248 627 3 619
Fragments arranged by size:
627 619 248 3
MspA1I CmG'CkG
Cuts at: 0 45 1497
Size: 45 1452
NaeI GCC'GGC
Cuts at: 0 156 1497
Size: 156 1341
NcoI C'CATG_G
Cuts at: 0 1251 1295 1497
Size: 1251 44 202
Fragments arranged by size:
1251 202 44
NgoMIV G'CCGG_C
Cuts at: 0 154 1497
Size: 154 1343
PflMI CCAn_nnn'nTGG
Cuts at: 0 1217 1497
Size: 1217 280
PfoI T'CCnGG_A
Cuts at: 0 524 1017 1497
Size: 524 493 480
Fragments arranged by size:
524 493 480
PpuMI rG'GwC_Cy
Cuts at: 0 1335 1497
Size: 1335 162
PspOMI G'GGCC_C
Cuts at: 0 950 1497
Size: 950 547
PstI C_TGCA'G
Cuts at: 0 653 1046 1497
Size: 653 393 451
Fragments arranged by size:
653 451 393
PvuII CAG'CTG
Cuts at: 0 45 1497
Size: 45 1452
SacI G_AGCT'C
Cuts at: 0 1241 1497
Size: 1241 256
ScaI AGT'ACT
Cuts at: 0 449 1497
Size: 449 1048
SfcI C'TryA_G
Cuts at: 0 453 649 1042 1497
Size: 453 196 393 455
Fragments arranged by size:
455 453 393 196
SmaI CCC'GGG
Cuts at: 0 1333 1497
Size: 1333 164
StyI C'CwwG_G
Cuts at: 0 95 194 1251 1295 1301 1485 1497
Size: 95 99 1057 44 6 184 12
Fragments arranged by size:
1057 184 99 95 44 12 6
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 69 138 1069 1497
Size: 69 69 931 428
Fragments arranged by size:
931 428 69 69
TatI w'GTAC_w
Cuts at: 0 447 588 1497
Size: 447 141 909
Fragments arranged by size:
909 447 141
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 934 1302 1497
Size: 934 368 195
Fragments arranged by size:
934 368 195
XmaI C'CCGG_G
Cuts at: 0 1331 1497
Size: 1331 166
Enzymes that do cut:
AcuI ApaI AvaI BanI BanII BbsI BbvCI BciVI
BglI Bme1580I BplI BpmI Bpu10I BsaI BsaHI BseYI
BsgI BsiHKAI Bsp1286I BspMI BsrBI BsrFI BssHII BstAPI
BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI
DraIII EaeI EcoICRI Eco57MI EcoNI EcoO109I HaeII Hin4I
MmeI MscI MslI MspA1I NaeI NcoI NgoMIV PflMI
PfoI PpuMI PspOMI PstI PvuII SacI ScaI SfcI
SmaI StyI TaqII TatI XcmI XmaI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI
AscI AseI AsiSI AvrII BaeI BamHI BbeI BcgI
BclI BfrBI BglII BlpI BmgBI BmrI BmtI BpuEI
BsaAI BsaBI BsaWI BsaXI BseRI BsiEI BsiWI BsmI
BsmBI BspEI BspHI BsrDI BsrGI BssSI BstZ17I ClaI
CspCI DraI DrdI EagI EarI EciI EcoRI EcoRV
FalI FseI FspI FspAI HincII HindIII HpaI KasI
KpnI MfeI MluI NarI NdeI NheI NotI NruI
NsiI NspI PacI PasI PciI PmeI PmlI PpiI
PshAI PsiI PspXI PsrI PvuI RsrII SacII SalI
SanDI SapI SbfI SexAI SfiI SfoI SgfI SgrAI
SmlI SnaBI SpeI SphI SrfI SspI StuI SwaI
TstI Tth111I XbaI XhoI XmnI ZraI