Restriction map for
pFN21AE1427
(Linear) (Six-base) MAPSORT of: KIEE1427.seq Check: 2757 from: 1 to: 3945
KIEE1427.orf
With 182 enzymes: SgfI *
July 15, 2010 18:38 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1274 1653 3945
Size: 1274 379 2292
Fragments arranged by size:
2292 1274 379
AccI GT'mk_AC
Cuts at: 0 1521 2272 3945
Size: 1521 751 1673
Fragments arranged by size:
1673 1521 751
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 115 314 826 1321 2093 2297 2884 3683
Size: 115 199 512 495 772 204 587 799
Cuts at: 3683 3945
Size: 262
Fragments arranged by size:
799 772 587 512 495 262 204 199
115
AflIII A'CryG_T
Cuts at: 0 14 3945
Size: 14 3931
AhdI GACnn_n'nnGTC
Cuts at: 0 549 3945
Size: 549 3396
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 2010 3442 3945
Size: 2010 1432 503
Fragments arranged by size:
2010 1432 503
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 830 862 1154 1186 3945
Size: 830 32 292 32 2759
Fragments arranged by size:
2759 830 292 32 32
AlwNI CAG_nnn'CTG
Cuts at: 0 674 699 726 967 1111 1266 1383 1590
Size: 674 25 27 241 144 155 117 207
Cuts at: 1590 1938 3166 3225 3807 3864 3945
Size: 348 1228 59 582 57 81
Fragments arranged by size:
1228 674 582 348 241 207 155 144
117 81 59 57 27 25
ApoI r'AATT_y
Cuts at: 0 138 3366 3945
Size: 138 3228 579
Fragments arranged by size:
3228 579 138
AvaI C'yCGr_G
Cuts at: 0 440 1030 1133 2674 3945
Size: 440 590 103 1541 1271
Fragments arranged by size:
1541 1271 590 440 103
AvrII C'CTAG_G
Cuts at: 0 3880 3945
Size: 3880 65
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 3796 3829 3945
Size: 3796 33 116
Fragments arranged by size:
3796 116 33
BamHI G'GATC_C
Cuts at: 0 3129 3492 3945
Size: 3129 363 453
Fragments arranged by size:
3129 453 363
BanI G'GyrC_C
Cuts at: 0 586 1119 2645 2907 3820 3945
Size: 586 533 1526 262 913 125
Fragments arranged by size:
1526 913 586 533 262 125
BanII G_rGCy'C
Cuts at: 0 260 1868 1920 2207 2810 3945
Size: 260 1608 52 287 603 1135
Fragments arranged by size:
1608 1135 603 287 260 52
BbeI G_GCGC'C
Cuts at: 0 2911 3945
Size: 2911 1034
BbsI GAAGACnn'nnnn_
Cuts at: 0 302 1435 2081 2182 3945
Size: 302 1133 646 101 1763
Fragments arranged by size:
1763 1133 646 302 101
BbvCI CC'TCA_GC
Cuts at: 0 133 248 929 3945
Size: 133 115 681 3016
Fragments arranged by size:
3016 681 133 115
BciVI GTATCCnnnnn_n'
Cuts at: 0 2857 3631 3945
Size: 2857 774 314
Fragments arranged by size:
2857 774 314
BglI GCCn_nnn'nGGC
Cuts at: 0 3353 3544 3945
Size: 3353 191 401
Fragments arranged by size:
3353 401 191
BlpI GC'TnA_GC
Cuts at: 0 2776 3460 3945
Size: 2776 684 485
Fragments arranged by size:
2776 684 485
Bme1580I G_kGCm'C
Cuts at: 0 1124 3381 3438 3584 3945
Size: 1124 2257 57 146 361
Fragments arranged by size:
2257 1124 361 146 57
BmrI ACTGGGnnnn_n'
Cuts at: 0 315 2805 3578 3945
Size: 315 2490 773 367
Fragments arranged by size:
2490 773 367 315
BmtI G_CTAG'C
Cuts at: 0 2600 3945
Size: 2600 1345
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1140 3945
Size: 1140 2805
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 488 1133 1391 1434 1537 1654 1812 1985
Size: 488 645 258 43 103 117 158 173
Cuts at: 1985 2440 3945
Size: 455 1505
Fragments arranged by size:
1505 645 488 455 258 173 158 117
103 43
Bpu10I CC'TnA_GC
Cuts at: 0 80 133 248 929 1435 2089 2729 2821
Size: 80 53 115 681 506 654 640 92
Cuts at: 2821 3945
Size: 1124
Fragments arranged by size:
1124 681 654 640 506 115 92 80
53
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 622 994 2128 3945
Size: 622 372 1134 1817
Fragments arranged by size:
1817 1134 622 372
BsaI GGTCTCn'nnnn_
Cuts at: 0 1267 3722 3945
Size: 1267 2455 223
Fragments arranged by size:
2455 1267 223
BsaAI yAC'GTr
Cuts at: 0 15 3945
Size: 15 3930
BsaHI Gr'CG_yC
Cuts at: 0 2908 3945
Size: 2908 1037
BsaWI w'CCGG_w
Cuts at: 0 1142 3770 3945
Size: 1142 2628 175
Fragments arranged by size:
2628 1142 175
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 2638 2668 2797 2827 3392 3422 3945
Size: 2638 30 129 30 565 30 523
Fragments arranged by size:
2638 565 523 129 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 385 532 946 1031 1192 1249 1298 1653
Size: 385 147 414 85 161 57 49 355
Cuts at: 1653 2566 2692 3691 3945
Size: 913 126 999 254
Fragments arranged by size:
999 913 414 385 355 254 161 147
126 85 57 49
BseYI C'CCAG_C
Cuts at: 0 1094 1194 2024 2834 2881 3280 3593 3701
Size: 1094 100 830 810 47 399 313 108
Cuts at: 3701 3734 3945
Size: 33 211
Fragments arranged by size:
1094 830 810 399 313 211 108 100
47 33
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1112 2119 2423 2672 3528 3945
Size: 1112 1007 304 249 856 417
Fragments arranged by size:
1112 1007 856 417 304 249
BsiHKAI G_wGCw'C
Cuts at: 0 81 260 454 1210 2945 3945
Size: 81 179 194 756 1735 1000
Fragments arranged by size:
1735 1000 756 194 179 81
BsmI GAATG_Cn'
Cuts at: 0 348 1168 1423 3945
Size: 348 820 255 2522
Fragments arranged by size:
2522 820 348 255
BsmBI CGTCTCn'nnnn_
Cuts at: 0 2394 3192 3945
Size: 2394 798 753
Fragments arranged by size:
2394 798 753
Bsp1286I G_dGCh'C
Cuts at: 0 81 260 454 1124 1210 1868 1920 2207
Size: 81 179 194 670 86 658 52 287
Cuts at: 2207 2810 2945 3381 3438 3584 3945
Size: 603 135 436 57 146 361
Fragments arranged by size:
670 658 603 436 361 287 194 179
146 135 86 81 57 52
BspEI T'CCGG_A
Cuts at: 0 3770 3945
Size: 3770 175
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 975 1274 1653 1839 2031 2333 3045 3570
Size: 975 299 379 186 192 302 712 525
Cuts at: 3570 3945
Size: 375
Fragments arranged by size:
975 712 525 379 375 302 299 192
186
BsrDI GCAATG_nn'
Cuts at: 0 311 3537 3636 3761 3945
Size: 311 3226 99 125 184
Fragments arranged by size:
3226 311 184 125 99
BsrGI T'GTAC_A
Cuts at: 0 53 3945
Size: 53 3892
BstAPI GCAn_nnn'nTGC
Cuts at: 0 699 1266 1743 3945
Size: 699 567 477 2202
Fragments arranged by size:
2202 699 567 477
BstEII G'GTnAC_C
Cuts at: 0 2442 3945
Size: 2442 1503
BstXI CCAn_nnnn'nTGG
Cuts at: 0 462 999 3601 3945
Size: 462 537 2602 344
Fragments arranged by size:
2602 537 462 344
BstYI r'GATC_y
Cuts at: 0 266 1067 1379 3129 3492 3606 3945
Size: 266 801 312 1750 363 114 339
Fragments arranged by size:
1750 801 363 339 312 266 114
Bsu36I CC'TnA_GG
Cuts at: 0 1043 2665 3022 3611 3925 3945
Size: 1043 1622 357 589 314 20
Fragments arranged by size:
1622 1043 589 357 314 20
BtgI C'CryG_G
Cuts at: 0 715 1787 2462 2657 2786 3945
Size: 715 1072 675 195 129 1159
Fragments arranged by size:
1159 1072 715 675 195 129
BtsI GCAGTG_nn'
Cuts at: 0 1464 1936 1997 2358 3436 3516 3584 3862
Size: 1464 472 61 361 1078 80 68 278
Cuts at: 3862 3900 3945
Size: 38 45
Fragments arranged by size:
1464 1078 472 361 278 80 68 61
45 38
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 390 425 3945
Size: 390 35 3520
Fragments arranged by size:
3520 390 35
DrdI GACnn_nn'nnGTC
Cuts at: 0 2429 3945
Size: 2429 1516
EaeI y'GGCC_r
Cuts at: 0 733 1061 2783 2789 3014 3945
Size: 733 328 1722 6 225 931
Fragments arranged by size:
1722 931 733 328 225 6
EarI CTCTTCn'nnn_
Cuts at: 0 1081 2261 3945
Size: 1081 1180 1684
Fragments arranged by size:
1684 1180 1081
EcoICRI GAG'CTC
Cuts at: 0 258 3945
Size: 258 3687
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 115 314 488 826 1133 1321 1391 1434
Size: 115 199 174 338 307 188 70 43
Cuts at: 1434 1537 1654 1812 1985 2093 2297 2440 2884
Size: 103 117 158 173 108 204 143 444
Cuts at: 2884 3683 3945
Size: 799 262
Fragments arranged by size:
799 444 338 307 262 204 199 188
174 173 158 143 117 115 108 103
70 43
EcoNI CCTnn'n_nnAGG
Cuts at: 0 400 2143 3781 3945
Size: 400 1743 1638 164
Fragments arranged by size:
1743 1638 400 164
EcoO109I rG'GnC_Cy
Cuts at: 0 642 809 846 964 1431 1574 1587 2178
Size: 642 167 37 118 467 143 13 591
Cuts at: 2178 3270 3559 3658 3945
Size: 1092 289 99 287
Fragments arranged by size:
1092 642 591 467 289 287 167 143
118 99 37 13
EcoRI G'AATT_C
Cuts at: 0 3366 3945
Size: 3366 579
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 2719 3945
Size: 2719 1226
FspI TGC'GCA
Cuts at: 0 3343 3945
Size: 3343 602
HaeII r_GCGC'y
Cuts at: 0 120 2911 3945
Size: 120 2791 1034
Fragments arranged by size:
2791 1034 120
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 535 567 1364 1396 2668 2700 3121 3153
Size: 535 32 797 32 1272 32 421 32
Cuts at: 3153 3229 3261 3392 3424 3820 3852 3945
Size: 76 32 131 32 396 32 93
Fragments arranged by size:
1272 797 535 421 396 131 93 76
32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 0 2519 3230 3945
Size: 2519 711 715
Fragments arranged by size:
2519 715 711
KasI G'GCGC_C
Cuts at: 0 2907 3945
Size: 2907 1038
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 3260 3435 3945
Size: 3260 175 510
Fragments arranged by size:
3260 510 175
MscI TGG'CCA
Cuts at: 0 735 2785 2791 3945
Size: 735 2050 6 1154
Fragments arranged by size:
2050 1154 735 6
MslI CAynn'nnrTG
Cuts at: 0 2381 3945
Size: 2381 1564
MspA1I CmG'CkG
Cuts at: 0 541 621 1094 1203 1359 1454 3574 3597
Size: 541 80 473 109 156 95 2120 23
Cuts at: 3597 3705 3945
Size: 108 240
Fragments arranged by size:
2120 541 473 240 156 109 108 95
80 23
NarI GG'CG_CC
Cuts at: 0 2908 3945
Size: 2908 1037
NcoI C'CATG_G
Cuts at: 0 1787 2462 2657 2786 3945
Size: 1787 675 195 129 1159
Fragments arranged by size:
1787 1159 675 195 129
NdeI CA'TA_TG
Cuts at: 0 194 1993 3945
Size: 194 1799 1952
Fragments arranged by size:
1952 1799 194
NheI G'CTAG_C
Cuts at: 0 2596 3945
Size: 2596 1349
NspI r_CATG'y
Cuts at: 0 2386 2491 3725 3945
Size: 2386 105 1234 220
Fragments arranged by size:
2386 1234 220 105
PasI CC'CwG_GG
Cuts at: 0 2757 3816 3945
Size: 2757 1059 129
Fragments arranged by size:
2757 1059 129
PflMI CCAn_nnn'nTGG
Cuts at: 0 18 873 2463 2468 2763 3945
Size: 18 855 1590 5 295 1182
Fragments arranged by size:
1590 1182 855 295 18 5
PmlI CAC'GTG
Cuts at: 0 15 3945
Size: 15 3930
PpuMI rG'GwC_Cy
Cuts at: 0 642 809 846 964 1431 1574 1587 3270
Size: 642 167 37 118 467 143 13 1683
Cuts at: 3270 3559 3658 3945
Size: 289 99 287
Fragments arranged by size:
1683 642 467 289 287 167 143 118
99 37 13
PstI C_TGCA'G
Cuts at: 0 695 1459 1664 2117 2481 2508 3056 3443
Size: 695 764 205 453 364 27 548 387
Cuts at: 3443 3579 3907 3945
Size: 136 328 38
Fragments arranged by size:
764 695 548 453 387 364 328 205
136 38 27
PvuII CAG'CTG
Cuts at: 0 621 1094 1359 1454 3574 3597 3945
Size: 621 473 265 95 2120 23 348
Fragments arranged by size:
2120 621 473 348 265 95 23
SacI G_AGCT'C
Cuts at: 0 260 3945
Size: 260 3685
SanDI GG'GwC_CC
Cuts at: 0 809 3945
Size: 809 3136
SapI GCTCTTCn'nnn_
Cuts at: 0 1081 3945
Size: 1081 2864
SbfI CC_TGCA'GG
Cuts at: 0 695 1664 3945
Size: 695 969 2281
Fragments arranged by size:
2281 969 695
SexAI A'CCwGG_T
Cuts at: 0 299 2526 3945
Size: 299 2227 1419
Fragments arranged by size:
2227 1419 299
SfcI C'TryA_G
Cuts at: 0 691 1455 1660 2113 2477 2504 3052 3439
Size: 691 764 205 453 364 27 548 387
Cuts at: 3439 3575 3903 3945
Size: 136 328 42
Fragments arranged by size:
764 691 548 453 387 364 328 205
136 42 27
SfoI GGC'GCC
Cuts at: 0 2909 3945
Size: 2909 1036
SmaI CCC'GGG
Cuts at: 0 442 3945
Size: 442 3503
SmlI C'TyrA_G
Cuts at: 0 637 973 2143 3945
Size: 637 336 1170 1802
Fragments arranged by size:
1802 1170 637 336
SrfI GCCC'GGGC
Cuts at: 0 442 3945
Size: 442 3503
StuI AGG'CCT
Cuts at: 0 913 1609 3885 3945
Size: 913 696 2276 60
Fragments arranged by size:
2276 913 696 60
StyI C'CwwG_G
Cuts at: 0 401 881 1787 1911 2033 2213 2462 2657
Size: 401 480 906 124 122 180 249 195
Cuts at: 2657 2786 3880 3945
Size: 129 1094 65
Fragments arranged by size:
1094 906 480 401 249 195 180 129
124 122 65
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 3374 3808 3945
Size: 3374 434 137
Fragments arranged by size:
3374 434 137
TatI w'GTAC_w
Cuts at: 0 53 338 3945
Size: 53 285 3607
Fragments arranged by size:
3607 285 53
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 2276 2308 2638 2670 3945
Size: 2276 32 330 32 1275
Fragments arranged by size:
2276 1275 330 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1689 2469 2911 3522 3945
Size: 1689 780 442 611 423
Fragments arranged by size:
1689 780 611 442 423
XmaI C'CCGG_G
Cuts at: 0 440 3945
Size: 440 3505
Enzymes that do cut:
AarI AccI AcuI AflIII AhdI AlfI AloI AlwNI
ApoI AvaI AvrII BaeI BamHI BanI BanII BbeI
BbsI BbvCI BciVI BglI BlpI Bme1580I BmrI BmtI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiHKAI BsmI BsmBI Bsp1286I
BspEI BspMI BsrDI BsrGI BstAPI BstEII BstXI BstYI
Bsu36I BtgI BtsI CspCI DrdI EaeI EarI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII KasI MmeI MscI MslI MspA1I NarI NcoI
NdeI NheI NspI PasI PflMI PmlI PpuMI PstI
PvuII SacI SanDI SapI SbfI SexAI SfcI SfoI
SmaI SmlI SrfI StuI StyI TaqII TatI TstI
XcmI XmaI
Enzymes that do not cut:
AatII Acc65I AclI AfeI AflII AgeI AleI ApaI
ApaLI AscI AseI AsiSI BcgI BclI BfrBI BglII
BmgBI BsaBI BsiEI BsiWI BspHI BsrBI BsrFI BssHII
BssSI BstBI BstZ17I BtgZI ClaI DraI DraIII EagI
EciI EcoRV FseI FspAI HindIII HpaI KpnI MfeI
MluI NaeI NgoMIV NotI NruI NsiI PacI PciI
PfoI PmeI PpiI PshAI PsiI PspOMI PspXI PsrI
PvuI RsrII SacII SalI ScaI SfiI SgfI SgrAI
SnaBI SpeI SphI SspI SwaI Tth111I XbaI XhoI
XmnI ZraI