Restriction map for
pFN21AE1425
(Linear) (Six-base) MAPSORT of: KIEE1425.seq Check: 9536 from: 1 to: 1092
KIEE1425.orf
With 182 enzymes: SgfI *
July 15, 2010 18:38 ..
AccI GT'mk_AC
Cuts at: 0 317 1092
Size: 317 775
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 286 318 1092
Size: 286 32 774
Fragments arranged by size:
774 286 32
ApaI G_GGCC'C
Cuts at: 0 42 565 1092
Size: 42 523 527
Fragments arranged by size:
527 523 42
ApoI r'AATT_y
Cuts at: 0 57 1092
Size: 57 1035
BamHI G'GATC_C
Cuts at: 0 51 282 1092
Size: 51 231 810
Fragments arranged by size:
810 231 51
BanI G'GyrC_C
Cuts at: 0 619 979 1092
Size: 619 360 113
Fragments arranged by size:
619 360 113
BanII G_rGCy'C
Cuts at: 0 42 565 1092
Size: 42 523 527
Fragments arranged by size:
527 523 42
BbsI GAAGACnn'nnnn_
Cuts at: 0 853 1092
Size: 853 239
Bme1580I G_kGCm'C
Cuts at: 0 42 119 565 1092
Size: 42 77 446 527
Fragments arranged by size:
527 446 77 42
BmrI ACTGGGnnnn_n'
Cuts at: 0 157 281 1092
Size: 157 124 811
Fragments arranged by size:
811 157 124
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 197 671 1092
Size: 197 474 421
Fragments arranged by size:
474 421 197
Bpu10I CC'TnA_GC
Cuts at: 0 307 741 1092
Size: 307 434 351
Fragments arranged by size:
434 351 307
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 951 1092
Size: 951 141
BsaI GGTCTCn'nnnn_
Cuts at: 0 748 1092
Size: 748 344
BsaHI Gr'CG_yC
Cuts at: 0 320 1092
Size: 320 772
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 88 118 142 172 286 316 644 674
Size: 88 30 24 30 114 30 328 30
Cuts at: 674 782 812 1092
Size: 108 30 280
Fragments arranged by size:
328 280 114 108 88 30 30 30
30 30 24
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 265 342 570 803 1021 1092
Size: 265 77 228 233 218 71
Fragments arranged by size:
265 233 228 218 77 71
BseYI C'CCAG_C
Cuts at: 0 395 1092
Size: 395 697
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 94 105 1092
Size: 94 11 987
Fragments arranged by size:
987 94 11
BsiHKAI G_wGCw'C
Cuts at: 0 590 1092
Size: 590 502
Bsp1286I G_dGCh'C
Cuts at: 0 42 119 565 590 1092
Size: 42 77 446 25 502
Fragments arranged by size:
502 446 77 42 25
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 493 1092
Size: 493 599
BssSI C'ACGA_G
Cuts at: 0 112 1092
Size: 112 980
BstXI CCAn_nnnn'nTGG
Cuts at: 0 62 558 1092
Size: 62 496 534
Fragments arranged by size:
534 496 62
BstYI r'GATC_y
Cuts at: 0 51 282 1092
Size: 51 231 810
Fragments arranged by size:
810 231 51
BtgI C'CryG_G
Cuts at: 0 601 615 1092
Size: 601 14 477
Fragments arranged by size:
601 477 14
BtsI GCAGTG_nn'
Cuts at: 0 884 1092
Size: 884 208
EaeI y'GGCC_r
Cuts at: 0 218 465 1092
Size: 218 247 627
Fragments arranged by size:
627 247 218
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 259 1092
Size: 259 833
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 197 671 1092
Size: 197 474 421
Fragments arranged by size:
474 421 197
EcoNI CCTnn'n_nnAGG
Cuts at: 0 905 1092
Size: 905 187
EcoO109I rG'GnC_Cy
Cuts at: 0 38 39 562 1085 1092
Size: 38 1 523 523 7
Fragments arranged by size:
523 523 38 7 1
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 142 174 257 289 1092
Size: 142 32 83 32 803
Fragments arranged by size:
803 142 83 32 32
MscI TGG'CCA
Cuts at: 0 220 1092
Size: 220 872
MspA1I CmG'CkG
Cuts at: 0 6 544 1092
Size: 6 538 548
Fragments arranged by size:
548 538 6
NspI r_CATG'y
Cuts at: 0 412 1092
Size: 412 680
PasI CC'CwG_GG
Cuts at: 0 558 1092
Size: 558 534
PflMI CCAn_nnn'nTGG
Cuts at: 0 169 718 736 1092
Size: 169 549 18 356
Fragments arranged by size:
549 356 169 18
PfoI T'CCnGG_A
Cuts at: 0 182 729 1092
Size: 182 547 363
Fragments arranged by size:
547 363 182
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 286 318 1092
Size: 286 32 774
Fragments arranged by size:
774 286 32
PpuMI rG'GwC_Cy
Cuts at: 0 1085 1092
Size: 1085 7
PspOMI G'GGCC_C
Cuts at: 0 38 561 1092
Size: 38 523 531
Fragments arranged by size:
531 523 38
PvuII CAG'CTG
Cuts at: 0 544 1092
Size: 544 548
SmlI C'TyrA_G
Cuts at: 0 966 1092
Size: 966 126
StyI C'CwwG_G
Cuts at: 0 276 1052 1092
Size: 276 776 40
Fragments arranged by size:
776 276 40
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 910 1092
Size: 910 182
TatI w'GTAC_w
Cuts at: 0 243 1092
Size: 243 849
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 88 120 644 676 684 716 1092
Size: 88 32 524 32 8 32 376
Fragments arranged by size:
524 376 88 32 32 32 8
XbaI T'CTAG_A
Cuts at: 0 456 1092
Size: 456 636
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 765 876 1092
Size: 765 111 216
Fragments arranged by size:
765 216 111
Enzymes that do cut:
AccI AloI ApaI ApoI BamHI BanI BanII BbsI
Bme1580I BmrI BpmI Bpu10I BpuEI BsaI BsaHI BsaXI
BseRI BseYI BsgI BsiHKAI Bsp1286I BspMI BssSI BstXI
BstYI BtgI BtsI EaeI EciI Eco57MI EcoNI EcoO109I
Hin4I MscI MspA1I NspI PasI PflMI PfoI PpiI
PpuMI PspOMI PvuII SmlI StyI TaqII TatI TstI
XbaI XcmI
Enzymes that do not cut:
AarI AatII Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI AscI AseI
AsiSI AvaI AvrII BaeI BbeI BbvCI BcgI BciVI
BclI BfrBI BglI BglII BlpI BmgBI BmtI BplI
BsaAI BsaBI BsaWI BsiEI BsiWI BsmI BsmBI BspEI
BspHI BsrBI BsrDI BsrFI BsrGI BssHII BstAPI BstBI
BstEII BstZ17I Bsu36I BtgZI ClaI CspCI DraI DraIII
DrdI EagI EarI EcoICRI EcoRI EcoRV FalI FseI
FspI FspAI HaeII HincII HindIII HpaI KasI KpnI
MfeI MluI MmeI MslI NaeI NarI NcoI NdeI
NgoMIV NheI NotI NruI NsiI PacI PciI PmeI
PmlI PshAI PsiI PspXI PsrI PstI PvuI RsrII
SacI SacII SalI SanDI SapI SbfI ScaI SexAI
SfcI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI
SphI SrfI SspI StuI SwaI Tth111I XhoI XmaI
XmnI ZraI