Restriction map for
pFN21AE1424
(Linear) (Six-base) MAPSORT of: KIEE1424.seq Check: 2014 from: 1 to: 1668
KIEE1424.orf
With 182 enzymes: SgfI *
July 3, 2012 16:53 ..
AclI AA'CG_TT
Cuts at: 0 62 1668
Size: 62 1606
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 205 257 699 910 1668
Size: 205 52 442 211 758
Fragments arranged by size:
758 442 211 205 52
AfeI AGC'GCT
Cuts at: 0 1168 1668
Size: 1168 500
AlwNI CAG_nnn'CTG
Cuts at: 0 769 1558 1668
Size: 769 789 110
Fragments arranged by size:
789 769 110
ApoI r'AATT_y
Cuts at: 0 332 821 1064 1543 1668
Size: 332 489 243 479 125
Fragments arranged by size:
489 479 332 243 125
AseI AT'TA_AT
Cuts at: 0 446 1668
Size: 446 1222
AvaI C'yCGr_G
Cuts at: 0 1295 1484 1668
Size: 1295 189 184
Fragments arranged by size:
1295 189 184
BamHI G'GATC_C
Cuts at: 0 661 1668
Size: 661 1007
BanI G'GyrC_C
Cuts at: 0 844 1668
Size: 844 824
BanII G_rGCy'C
Cuts at: 0 1659 1668
Size: 1659 9
BbsI GAAGACnn'nnnn_
Cuts at: 0 461 1668
Size: 461 1207
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 353 387 1668
Size: 353 34 1281
Fragments arranged by size:
1281 353 34
BglII A'GATC_T
Cuts at: 0 1383 1668
Size: 1383 285
BmrI ACTGGGnnnn_n'
Cuts at: 0 47 436 1668
Size: 47 389 1232
Fragments arranged by size:
1232 389 47
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 214 989 1668
Size: 214 775 679
Fragments arranged by size:
775 679 214
BsaI GGTCTCn'nnnn_
Cuts at: 0 1298 1668
Size: 1298 370
BsaAI yAC'GTr
Cuts at: 0 1132 1668
Size: 1132 536
BsaBI GATnn'nnATC
Cuts at: 0 1087 1668
Size: 1087 581
BsiWI C'GTAC_G
Cuts at: 0 1108 1668
Size: 1108 560
Bsp1286I G_dGCh'C
Cuts at: 0 1659 1668
Size: 1659 9
BsrBI CCG'CTC
Cuts at: 0 53 1668
Size: 53 1615
BsrDI GCAATG_nn'
Cuts at: 0 953 1034 1479 1668
Size: 953 81 445 189
Fragments arranged by size:
953 445 189 81
BsrGI T'GTAC_A
Cuts at: 0 747 1668
Size: 747 921
BstAPI GCAn_nnn'nTGC
Cuts at: 0 700 844 1321 1363 1668
Size: 700 144 477 42 305
Fragments arranged by size:
700 477 305 144 42
BstBI TT'CG_AA
Cuts at: 0 1575 1668
Size: 1575 93
BstEII G'GTnAC_C
Cuts at: 0 25 1668
Size: 25 1643
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1386 1668
Size: 1386 282
BstYI r'GATC_y
Cuts at: 0 661 830 1383 1668
Size: 661 169 553 285
Fragments arranged by size:
661 553 285 169
BtgI C'CryG_G
Cuts at: 0 600 1668
Size: 600 1068
BtsI GCAGTG_nn'
Cuts at: 0 389 1668
Size: 389 1279
ClaI AT'CG_AT
Cuts at: 0 1120 1668
Size: 1120 548
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1202 1237 1668
Size: 1202 35 431
Fragments arranged by size:
1202 431 35
DraI TTT'AAA
Cuts at: 0 935 1668
Size: 935 733
DraIII CAC_nnn'GTG
Cuts at: 0 1189 1668
Size: 1189 479
DrdI GACnn_nn'nnGTC
Cuts at: 0 465 1668
Size: 465 1203
EaeI y'GGCC_r
Cuts at: 0 992 1668
Size: 992 676
EarI CTCTTCn'nnn_
Cuts at: 0 426 921 1192 1668
Size: 426 495 271 476
Fragments arranged by size:
495 476 426 271
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 205 214 257 699 910 989 1668
Size: 205 9 43 442 211 79 679
Fragments arranged by size:
679 442 211 205 79 43 9
EcoO109I rG'GnC_Cy
Cuts at: 0 7 942 1668
Size: 7 935 726
Fragments arranged by size:
935 726 7
EcoRI G'AATT_C
Cuts at: 0 821 1668
Size: 821 847
EcoRV GAT'ATC
Cuts at: 0 807 1668
Size: 807 861
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1270 1668
Size: 1270 398
HaeII r_GCGC'y
Cuts at: 0 1170 1668
Size: 1170 498
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1201 1233 1668
Size: 1201 32 435
Fragments arranged by size:
1201 435 32
HincII GTy'rAC
Cuts at: 0 414 576 986 1428 1668
Size: 414 162 410 442 240
Fragments arranged by size:
442 414 410 240 162
HindIII A'AGCT_T
Cuts at: 0 739 962 1668
Size: 739 223 706
Fragments arranged by size:
739 706 223
HpaI GTT'AAC
Cuts at: 0 414 1428 1668
Size: 414 1014 240
Fragments arranged by size:
1014 414 240
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 237 1668
Size: 237 1431
MscI TGG'CCA
Cuts at: 0 994 1668
Size: 994 674
NcoI C'CATG_G
Cuts at: 0 600 1668
Size: 600 1068
NspI r_CATG'y
Cuts at: 0 675 1668
Size: 675 993
PasI CC'CwG_GG
Cuts at: 0 666 1668
Size: 666 1002
PpuMI rG'GwC_Cy
Cuts at: 0 942 1668
Size: 942 726
PstI C_TGCA'G
Cuts at: 0 75 213 1668
Size: 75 138 1455
Fragments arranged by size:
1455 138 75
SfcI C'TryA_G
Cuts at: 0 71 209 1241 1668
Size: 71 138 1032 427
Fragments arranged by size:
1032 427 138 71
SmlI C'TyrA_G
Cuts at: 0 1484 1668
Size: 1484 184
SphI G_CATG'C
Cuts at: 0 675 1668
Size: 675 993
SspI AAT'ATT
Cuts at: 0 90 588 1668
Size: 90 498 1080
Fragments arranged by size:
1080 498 90
StyI C'CwwG_G
Cuts at: 0 600 812 1668
Size: 600 212 856
Fragments arranged by size:
856 600 212
TatI w'GTAC_w
Cuts at: 0 525 747 1668
Size: 525 222 921
Fragments arranged by size:
921 525 222
XhoI C'TCGA_G
Cuts at: 0 1484 1668
Size: 1484 184
XmnI GAAnn'nnTTC
Cuts at: 0 270 1668
Size: 270 1398
Enzymes that do cut:
AclI AcuI AfeI AlwNI ApoI AseI AvaI BamHI
BanI BanII BbsI BcgI BglII BmrI BpmI BsaI
BsaAI BsaBI BsiWI Bsp1286I BsrBI BsrDI BsrGI BstAPI
BstBI BstEII BstXI BstYI BtgI BtsI ClaI CspCI
DraI DraIII DrdI EaeI EarI Eco57MI EcoO109I EcoRI
EcoRV FalI HaeII Hin4I HincII HindIII HpaI MmeI
MscI NcoI NspI PasI PpuMI PstI SfcI SmlI
SphI SspI StyI TatI XhoI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AflII AflIII AgeI AhdI
AleI AlfI AloI ApaI ApaLI AscI AsiSI AvrII
BaeI BbeI BbvCI BciVI BclI BfrBI BglI BlpI
Bme1580I BmgBI BmtI BplI Bpu10I BpuEI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI
BspEI BspHI BspMI BsrFI BssHII BssSI BstZ17I Bsu36I
BtgZI EagI EciI EcoICRI EcoNI FseI FspI FspAI
KasI KpnI MfeI MluI MslI MspA1I NaeI NarI
NdeI NgoMIV NheI NotI NruI NsiI PacI PciI
PflMI PfoI PmeI PmlI PpiI PshAI PsiI PspOMI
PspXI PsrI PvuI PvuII RsrII SacI SacII SalI
SanDI SapI SbfI ScaI SexAI SfiI SfoI SgfI
SgrAI SmaI SnaBI SpeI SrfI StuI SwaI TaqII
TstI Tth111I XbaI XcmI XmaI ZraI