Restriction map for
pFN21AE1422
(Linear) (Six-base) MAPSORT of: KIEE1422.seq Check: 5926 from: 1 to: 1275
KIEE1422.orf
With 182 enzymes: SgfI *
July 15, 2010 18:38 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 727 1275
Size: 727 548
AfeI AGC'GCT
Cuts at: 0 1032 1222 1275
Size: 1032 190 53
Fragments arranged by size:
1032 190 53
ApaLI G'TGCA_C
Cuts at: 0 1261 1275
Size: 1261 14
AvaI C'yCGr_G
Cuts at: 0 69 1095 1275
Size: 69 1026 180
Fragments arranged by size:
1026 180 69
AvrII C'CTAG_G
Cuts at: 0 117 1275
Size: 117 1158
BanI G'GyrC_C
Cuts at: 0 532 1275
Size: 532 743
BanII G_rGCy'C
Cuts at: 0 76 265 1275
Size: 76 189 1010
Fragments arranged by size:
1010 189 76
BbsI GAAGACnn'nnnn_
Cuts at: 0 653 953 1275
Size: 653 300 322
Fragments arranged by size:
653 322 300
Bme1580I G_kGCm'C
Cuts at: 0 1265 1275
Size: 1265 10
BmgBI CAC'GTC
Cuts at: 0 827 1275
Size: 827 448
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 364 445 598 1065 1240 1275
Size: 364 81 153 467 175 35
Fragments arranged by size:
467 364 175 153 81 35
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 709 1275
Size: 709 566
BsaI GGTCTCn'nnnn_
Cuts at: 0 928 1275
Size: 928 347
BsaWI w'CCGG_w
Cuts at: 0 529 558 1275
Size: 529 29 717
Fragments arranged by size:
717 529 29
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 298 328 1255 1275
Size: 298 30 927 20
Fragments arranged by size:
927 298 30 20
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 145 436 565 597 649 813 1003 1072
Size: 145 291 129 32 52 164 190 69
Cuts at: 1072 1156 1159 1225 1275
Size: 84 3 66 50
Fragments arranged by size:
291 190 164 145 129 84 69 66
52 50 32 3
BseYI C'CCAG_C
Cuts at: 0 76 106 440 771 1275
Size: 76 30 334 331 504
Fragments arranged by size:
504 334 331 76 30
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 235 574 1261 1275
Size: 235 339 687 14
Fragments arranged by size:
687 339 235 14
BsiHKAI G_wGCw'C
Cuts at: 0 176 473 1256 1265 1275
Size: 176 297 783 9 10
Fragments arranged by size:
783 297 176 10 9
Bsp1286I G_dGCh'C
Cuts at: 0 76 176 265 473 1256 1265 1275
Size: 76 100 89 208 783 9 10
Fragments arranged by size:
783 208 100 89 76 10 9
BspEI T'CCGG_A
Cuts at: 0 558 1275
Size: 558 717
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 959 1275
Size: 959 316
BssHII G'CGCG_C
Cuts at: 0 375 1275
Size: 375 900
BstXI CCAn_nnnn'nTGG
Cuts at: 0 40 1035 1275
Size: 40 995 240
Fragments arranged by size:
995 240 40
BstYI r'GATC_y
Cuts at: 0 494 697 1275
Size: 494 203 578
Fragments arranged by size:
578 494 203
Bsu36I CC'TnA_GG
Cuts at: 0 849 1275
Size: 849 426
BtgI C'CryG_G
Cuts at: 0 536 596 1275
Size: 536 60 679
Fragments arranged by size:
679 536 60
BtsI GCAGTG_nn'
Cuts at: 0 409 966 1275
Size: 409 557 309
Fragments arranged by size:
557 409 309
DraIII CAC_nnn'GTG
Cuts at: 0 597 1275
Size: 597 678
EarI CTCTTCn'nnn_
Cuts at: 0 980 1133 1275
Size: 980 153 142
Fragments arranged by size:
980 153 142
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 909 1231 1275
Size: 909 322 44
Fragments arranged by size:
909 322 44
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 364 445 598 727 1065 1240 1275
Size: 364 81 153 129 338 175 35
Fragments arranged by size:
364 338 175 153 129 81 35
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1162 1275
Size: 1162 113
EcoO109I rG'GnC_Cy
Cuts at: 0 639 1233 1275
Size: 639 594 42
Fragments arranged by size:
639 594 42
FspI TGC'GCA
Cuts at: 0 408 1275
Size: 408 867
FspAI rTGC'GCAy
Cuts at: 0 408 1275
Size: 408 867
HaeII r_GCGC'y
Cuts at: 0 368 1034 1224 1275
Size: 368 666 190 51
Fragments arranged by size:
666 368 190 51
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 592 624 925 957 1132 1164 1275
Size: 592 32 301 32 175 32 111
Fragments arranged by size:
592 301 175 111 32 32 32
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 474 1275
Size: 474 801
NspI r_CATG'y
Cuts at: 0 54 204 1275
Size: 54 150 1071
Fragments arranged by size:
1071 150 54
PasI CC'CwG_GG
Cuts at: 0 40 1275
Size: 40 1235
PfoI T'CCnGG_A
Cuts at: 0 140 182 479 1275
Size: 140 42 297 796
Fragments arranged by size:
796 297 140 42
PpuMI rG'GwC_Cy
Cuts at: 0 1233 1275
Size: 1233 42
PspXI vC'TCGA_Gb
Cuts at: 0 69 1275
Size: 69 1206
SanDI GG'GwC_CC
Cuts at: 0 1233 1275
Size: 1233 42
SfcI C'TryA_G
Cuts at: 0 791 1275
Size: 791 484
SmlI C'TyrA_G
Cuts at: 0 69 724 1275
Size: 69 655 551
Fragments arranged by size:
655 551 69
StuI AGG'CCT
Cuts at: 0 313 1275
Size: 313 962
StyI C'CwwG_G
Cuts at: 0 117 1275
Size: 117 1158
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 388 420 1255 1275
Size: 388 32 835 20
Fragments arranged by size:
835 388 32 20
Tth111I GACn'n_nGTC
Cuts at: 0 939 1275
Size: 939 336
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1035 1275
Size: 1035 240
XhoI C'TCGA_G
Cuts at: 0 69 1275
Size: 69 1206
Enzymes that do cut:
AcuI AfeI ApaLI AvaI AvrII BanI BanII BbsI
Bme1580I BmgBI BpmI BpuEI BsaI BsaWI BsaXI BseRI
BseYI BsgI BsiHKAI Bsp1286I BspEI BspMI BssHII BstXI
BstYI Bsu36I BtgI BtsI DraIII EarI EciI Eco57MI
EcoNI EcoO109I FspI FspAI HaeII Hin4I MmeI NspI
PasI PfoI PpuMI PspXI SanDI SfcI SmlI StuI
StyI TstI Tth111I XcmI XhoI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaI ApoI AscI
AseI AsiSI BaeI BamHI BbeI BbvCI BcgI BciVI
BclI BfrBI BglI BglII BlpI BmrI BmtI BplI
Bpu10I BsaAI BsaBI BsaHI BsiEI BsiWI BsmI BsmBI
BspHI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI
BstEII BstZ17I BtgZI ClaI CspCI DraI DrdI EaeI
EagI EcoICRI EcoRI EcoRV FalI FseI HincII HindIII
HpaI KasI KpnI MfeI MluI MscI MslI MspA1I
NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI
NsiI PacI PciI PflMI PmeI PmlI PpiI PshAI
PsiI PspOMI PsrI PstI PvuI PvuII RsrII SacI
SacII SalI SapI SbfI ScaI SexAI SfiI SfoI
SgfI SgrAI SmaI SnaBI SpeI SphI SrfI SspI
SwaI TaqII TatI XbaI XmaI XmnI ZraI