Restriction map for
pFN21AE1419
(Linear) (Six-base) MAPSORT of: KIEE1419.seq Check: 1926 from: 1 to: 831
KIEE1419.orf
With 182 enzymes: SgfI *
July 15, 2010 18:38 ..
AgeI A'CCGG_T
Cuts at: 0 777 831
Size: 777 54
AlwNI CAG_nnn'CTG
Cuts at: 0 138 831
Size: 138 693
ApoI r'AATT_y
Cuts at: 0 196 831
Size: 196 635
AvrII C'CTAG_G
Cuts at: 0 258 831
Size: 258 573
BamHI G'GATC_C
Cuts at: 0 608 831
Size: 608 223
BanI G'GyrC_C
Cuts at: 0 283 831
Size: 283 548
BanII G_rGCy'C
Cuts at: 0 434 582 831
Size: 434 148 249
Fragments arranged by size:
434 249 148
BglI GCCn_nnn'nGGC
Cuts at: 0 443 831
Size: 443 388
Bme1580I G_kGCm'C
Cuts at: 0 286 831
Size: 286 545
BmrI ACTGGGnnnn_n'
Cuts at: 0 433 831
Size: 433 398
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 731 831
Size: 731 100
BsaI GGTCTCn'nnnn_
Cuts at: 0 496 831
Size: 496 335
BsaBI GATnn'nnATC
Cuts at: 0 595 831
Size: 595 236
BsaWI w'CCGG_w
Cuts at: 0 777 831
Size: 777 54
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 729 759 831
Size: 729 30 72
Fragments arranged by size:
729 72 30
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 135 291 346 772 831
Size: 135 156 55 426 59
Fragments arranged by size:
426 156 135 59 55
BsmI GAATG_Cn'
Cuts at: 0 178 831
Size: 178 653
Bsp1286I G_dGCh'C
Cuts at: 0 286 434 582 831
Size: 286 148 148 249
Fragments arranged by size:
286 249 148 148
BsrBI CCG'CTC
Cuts at: 0 368 831
Size: 368 463
BsrFI r'CCGG_y
Cuts at: 0 777 831
Size: 777 54
BstBI TT'CG_AA
Cuts at: 0 473 831
Size: 473 358
BstEII G'GTnAC_C
Cuts at: 0 780 831
Size: 780 51
BstXI CCAn_nnnn'nTGG
Cuts at: 0 513 627 793 831
Size: 513 114 166 38
Fragments arranged by size:
513 166 114 38
BstYI r'GATC_y
Cuts at: 0 608 831
Size: 608 223
BtsI GCAGTG_nn'
Cuts at: 0 25 68 225 737 831
Size: 25 43 157 512 94
Fragments arranged by size:
512 157 94 43 25
EaeI y'GGCC_r
Cuts at: 0 653 831
Size: 653 178
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 731 831
Size: 731 100
EcoO109I rG'GnC_Cy
Cuts at: 0 675 831
Size: 675 156
EcoRI G'AATT_C
Cuts at: 0 196 831
Size: 196 635
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 758 831
Size: 758 73
MscI TGG'CCA
Cuts at: 0 655 831
Size: 655 176
MslI CAynn'nnrTG
Cuts at: 0 814 831
Size: 814 17
MspA1I CmG'CkG
Cuts at: 0 49 831
Size: 49 782
NspI r_CATG'y
Cuts at: 0 77 831
Size: 77 754
PshAI GACnn'nnGTC
Cuts at: 0 49 831
Size: 49 782
PstI C_TGCA'G
Cuts at: 0 20 831
Size: 20 811
SexAI A'CCwGG_T
Cuts at: 0 512 831
Size: 512 319
SfcI C'TryA_G
Cuts at: 0 16 404 831
Size: 16 388 427
Fragments arranged by size:
427 388 16
SphI G_CATG'C
Cuts at: 0 77 831
Size: 77 754
StuI AGG'CCT
Cuts at: 0 343 831
Size: 343 488
StyI C'CwwG_G
Cuts at: 0 258 487 831
Size: 258 229 344
Fragments arranged by size:
344 258 229
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 81 799 831
Size: 81 718 32
Fragments arranged by size:
718 81 32
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 483 515 729 761 831
Size: 483 32 214 32 70
Fragments arranged by size:
483 214 70 32 32
XmnI GAAnn'nnTTC
Cuts at: 0 176 831
Size: 176 655
Enzymes that do cut:
AgeI AlwNI ApoI AvrII BamHI BanI BanII BglI
Bme1580I BmrI BpmI BsaI BsaBI BsaWI BsaXI BsgI
BsmI Bsp1286I BsrBI BsrFI BstBI BstEII BstXI BstYI
BtsI EaeI Eco57MI EcoO109I EcoRI MmeI MscI MslI
MspA1I NspI PshAI PstI SexAI SfcI SphI StuI
StyI TaqII TstI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AhdI AleI AlfI AloI ApaI ApaLI AscI
AseI AsiSI AvaI BaeI BbeI BbsI BbvCI BcgI
BciVI BclI BfrBI BglII BlpI BmgBI BmtI BplI
Bpu10I BpuEI BsaAI BsaHI BseRI BseYI BsiEI BsiHKAI
BsiWI BsmBI BspEI BspHI BspMI BsrDI BsrGI BssHII
BssSI BstAPI BstZ17I Bsu36I BtgI BtgZI ClaI CspCI
DraI DraIII DrdI EagI EarI EciI EcoICRI EcoNI
EcoRV FalI FseI FspI FspAI HaeII Hin4I HincII
HindIII HpaI KasI KpnI MfeI MluI NaeI NarI
NcoI NdeI NgoMIV NheI NotI NruI NsiI PacI
PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI
PsiI PspOMI PspXI PsrI PvuI PvuII RsrII SacI
SacII SalI SanDI SapI SbfI ScaI SfiI SfoI
SgfI SgrAI SmaI SmlI SnaBI SpeI SrfI SspI
SwaI TatI Tth111I XbaI XcmI XhoI XmaI ZraI