Restriction map for
pFN21AE1417
(Linear) (Six-base) MAPSORT of: KIEE1417.seq Check: 2427 from: 1 to: 1758
KIEE1417.orf
With 182 enzymes: SgfI *
July 15, 2010 18:38 ..
Acc65I G'GTAC_C
Cuts at: 0 434 1758
Size: 434 1324
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 196 278 595 1758
Size: 196 82 317 1163
Fragments arranged by size:
1163 317 196 82
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1268 1758
Size: 1268 490
AlwNI CAG_nnn'CTG
Cuts at: 0 591 1593 1758
Size: 591 1002 165
Fragments arranged by size:
1002 591 165
ApaI G_GGCC'C
Cuts at: 0 1116 1758
Size: 1116 642
ApaLI G'TGCA_C
Cuts at: 0 421 1758
Size: 421 1337
AvaI C'yCGr_G
Cuts at: 0 926 1622 1758
Size: 926 696 136
Fragments arranged by size:
926 696 136
BamHI G'GATC_C
Cuts at: 0 512 836 1758
Size: 512 324 922
Fragments arranged by size:
922 512 324
BanI G'GyrC_C
Cuts at: 0 434 789 1758
Size: 434 355 969
Fragments arranged by size:
969 434 355
BanII G_rGCy'C
Cuts at: 0 278 1094 1116 1487 1758
Size: 278 816 22 371 271
Fragments arranged by size:
816 371 278 271 22
BbsI GAAGACnn'nnnn_
Cuts at: 0 1075 1378 1758
Size: 1075 303 380
Fragments arranged by size:
1075 380 303
BbvCI CC'TCA_GC
Cuts at: 0 269 439 1758
Size: 269 170 1319
Fragments arranged by size:
1319 269 170
BclI T'GATC_A
Cuts at: 0 90 1758
Size: 90 1668
BglII A'GATC_T
Cuts at: 0 341 1758
Size: 341 1417
BlpI GC'TnA_GC
Cuts at: 0 1600 1758
Size: 1600 158
Bme1580I G_kGCm'C
Cuts at: 0 425 1116 1146 1758
Size: 425 691 30 612
Fragments arranged by size:
691 612 425 30
BmgBI CAC'GTC
Cuts at: 0 817 1758
Size: 817 941
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 67 211 973 1144 1228 1758
Size: 67 144 762 171 84 530
Fragments arranged by size:
762 530 171 144 84 67
Bpu10I CC'TnA_GC
Cuts at: 0 269 331 439 584 1196 1758
Size: 269 62 108 145 612 562
Fragments arranged by size:
612 562 269 145 108 62
BsaWI w'CCGG_w
Cuts at: 0 509 1758
Size: 509 1249
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 286 946 1048 1078 1141 1144 1165 1216
Size: 286 660 102 30 63 3 21 51
Cuts at: 1216 1396 1555 1758
Size: 180 159 203
Fragments arranged by size:
660 286 203 180 159 102 63 51
30 21 3
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 409 925 1169 1372 1600 1758
Size: 409 516 244 203 228 158
Fragments arranged by size:
516 409 244 228 203 158
BsiHKAI G_wGCw'C
Cuts at: 0 278 371 425 1094 1758
Size: 278 93 54 669 664
Fragments arranged by size:
669 664 278 93 54
Bsp1286I G_dGCh'C
Cuts at: 0 278 371 425 1094 1116 1146 1487 1758
Size: 278 93 54 669 22 30 341 271
Fragments arranged by size:
669 341 278 271 93 54 30 22
BsrBI CCG'CTC
Cuts at: 0 450 957 1758
Size: 450 507 801
Fragments arranged by size:
801 507 450
BsrDI GCAATG_nn'
Cuts at: 0 1542 1758
Size: 1542 216
BsrFI r'CCGG_y
Cuts at: 0 1437 1758
Size: 1437 321
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1020 1758
Size: 1020 738
BstYI r'GATC_y
Cuts at: 0 341 512 836 1709 1758
Size: 341 171 324 873 49
Fragments arranged by size:
873 341 324 171 49
BtgI C'CryG_G
Cuts at: 0 32 537 995 1487 1758
Size: 32 505 458 492 271
Fragments arranged by size:
505 492 458 271 32
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 350 1758
Size: 350 1408
BtsI GCAGTG_nn'
Cuts at: 0 1186 1271 1758
Size: 1186 85 487
Fragments arranged by size:
1186 487 85
DraIII CAC_nnn'GTG
Cuts at: 0 147 1395 1758
Size: 147 1248 363
Fragments arranged by size:
1248 363 147
EarI CTCTTCn'nnn_
Cuts at: 0 1289 1322 1758
Size: 1289 33 436
Fragments arranged by size:
1289 436 33
EcoICRI GAG'CTC
Cuts at: 0 276 1092 1758
Size: 276 816 666
Fragments arranged by size:
816 666 276
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 67 196 211 278 595 973 1144 1228
Size: 67 129 15 67 317 378 171 84
Cuts at: 1228 1758
Size: 530
Fragments arranged by size:
530 378 317 171 129 84 67 67
15
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1294 1758
Size: 1294 464
EcoO109I rG'GnC_Cy
Cuts at: 0 377 588 1112 1113 1640 1752 1758
Size: 377 211 524 1 527 112 6
Fragments arranged by size:
527 524 377 211 112 6 1
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 346 732 1758
Size: 346 386 1026
Fragments arranged by size:
1026 386 346
HaeII r_GCGC'y
Cuts at: 0 1164 1758
Size: 1164 594
HincII GTy'rAC
Cuts at: 0 701 1758
Size: 701 1057
HpaI GTT'AAC
Cuts at: 0 701 1758
Size: 701 1057
KpnI G_GTAC'C
Cuts at: 0 438 1758
Size: 438 1320
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 545 1758
Size: 545 1213
MslI CAynn'nnrTG
Cuts at: 0 901 1758
Size: 901 857
MspA1I CmG'CkG
Cuts at: 0 951 966 1122 1248 1584 1590 1758
Size: 951 15 156 126 336 6 168
Fragments arranged by size:
951 336 168 156 126 15 6
NcoI C'CATG_G
Cuts at: 0 32 1758
Size: 32 1726
NspI r_CATG'y
Cuts at: 0 529 1758
Size: 529 1229
PflMI CCAn_nnn'nTGG
Cuts at: 0 147 712 1758
Size: 147 565 1046
Fragments arranged by size:
1046 565 147
PfoI T'CCnGG_A
Cuts at: 0 116 281 1289 1454 1523 1758
Size: 116 165 1008 165 69 235
Fragments arranged by size:
1008 235 165 165 116 69
PpuMI rG'GwC_Cy
Cuts at: 0 377 588 1640 1758
Size: 377 211 1052 118
Fragments arranged by size:
1052 377 211 118
PspOMI G'GGCC_C
Cuts at: 0 1112 1758
Size: 1112 646
PstI C_TGCA'G
Cuts at: 0 845 1055 1121 1758
Size: 845 210 66 637
Fragments arranged by size:
845 637 210 66
PvuII CAG'CTG
Cuts at: 0 951 966 1122 1248 1590 1758
Size: 951 15 156 126 342 168
Fragments arranged by size:
951 342 168 156 126 15
SacI G_AGCT'C
Cuts at: 0 278 1094 1758
Size: 278 816 664
Fragments arranged by size:
816 664 278
SexAI A'CCwGG_T
Cuts at: 0 1364 1391 1758
Size: 1364 27 367
Fragments arranged by size:
1364 367 27
SfcI C'TryA_G
Cuts at: 0 841 1051 1117 1494 1758
Size: 841 210 66 377 264
Fragments arranged by size:
841 377 264 210 66
SmaI CCC'GGG
Cuts at: 0 928 1758
Size: 928 830
SphI G_CATG'C
Cuts at: 0 529 1758
Size: 529 1229
SspI AAT'ATT
Cuts at: 0 678 1758
Size: 678 1080
StyI C'CwwG_G
Cuts at: 0 32 666 721 1376 1758
Size: 32 634 55 655 382
Fragments arranged by size:
655 634 382 55 32
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 307 1758
Size: 307 1451
TatI w'GTAC_w
Cuts at: 0 1691 1758
Size: 1691 67
XmaI C'CCGG_G
Cuts at: 0 926 1758
Size: 926 832
XmnI GAAnn'nnTTC
Cuts at: 0 301 1758
Size: 301 1457
Enzymes that do cut:
Acc65I AcuI AlfI AlwNI ApaI ApaLI AvaI BamHI
BanI BanII BbsI BbvCI BclI BglII BlpI Bme1580I
BmgBI BpmI Bpu10I BsaWI BseRI BsgI BsiHKAI Bsp1286I
BsrBI BsrDI BsrFI BstAPI BstYI BtgI BtgZI BtsI
DraIII EarI EcoICRI Eco57MI EcoNI EcoO109I FalI HaeII
HincII HpaI KpnI MmeI MslI MspA1I NcoI NspI
PflMI PfoI PpuMI PspOMI PstI PvuII SacI SexAI
SfcI SmaI SphI SspI StyI TaqII TatI XmaI
XmnI
Enzymes that do not cut:
AarI AatII AccI AclI AfeI AflII AflIII AgeI
AhdI AleI AloI ApoI AscI AseI AsiSI AvrII
BaeI BbeI BcgI BciVI BfrBI BglI BmrI BmtI
BplI BpuEI BsaI BsaAI BsaBI BsaHI BsaXI BseYI
BsiEI BsiWI BsmI BsmBI BspEI BspHI BspMI BsrGI
BssHII BssSI BstBI BstEII BstXI BstZ17I Bsu36I ClaI
CspCI DraI DrdI EaeI EagI EciI EcoRI EcoRV
FseI FspI FspAI Hin4I HindIII KasI MfeI MluI
MscI NaeI NarI NdeI NgoMIV NheI NotI NruI
NsiI PacI PasI PciI PmeI PmlI PpiI PshAI
PsiI PspXI PsrI PvuI RsrII SacII SalI SanDI
SapI SbfI ScaI SfiI SfoI SgfI SgrAI SmlI
SnaBI SpeI SrfI StuI SwaI TstI Tth111I XbaI
XcmI XhoI ZraI