(Linear) (Six-base) MAPSORT of: KIEE1416.seq  Check: 5498  from: 1  to: 321



KIEE1416.orf

 With 182 enzymes: SgfI * 

                        July 15, 2010 18:38  ..

AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     172     204     321 
   Size:        172      32     117

  Fragments arranged by size: 

                 172     117      32
 
AlwNI CAG_nnn'CTG

Cuts at:      0     144     321 
   Size:        144     177
 
ApoI r'AATT_y

Cuts at:      0     152     321 
   Size:        152     169
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     217     321 
   Size:        217     104
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0      47      77      92     122     321 
   Size:         47      30      15      30     199

  Fragments arranged by size: 

                 199      47      30      30      15
 
BseYI C'CCAG_C

Cuts at:      0      28     321 
   Size:         28     293
 
BsrFI r'CCGG_y

Cuts at:      0      86     321 
   Size:         86     235
 
BssHII G'CGCG_C

Cuts at:      0      90     321 
   Size:         90     231
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     144     321 
   Size:        144     177
 
BtgI C'CryG_G

Cuts at:      0     107     321 
   Size:        107     214
 
BtsI GCAGTG_nn'

Cuts at:      0      55     201     321 
   Size:         55     146     120

  Fragments arranged by size: 

                 146     120      55
 
EaeI y'GGCC_r

Cuts at:      0      84     110     321 
   Size:         84      26     211

  Fragments arranged by size: 

                 211      84      26
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0       9     258     321 
   Size:          9     249      63

  Fragments arranged by size: 

                 249      63       9
 
EcoRI G'AATT_C

Cuts at:      0     152     321 
   Size:        152     169
 
HindIII A'AGCT_T

Cuts at:      0     248     321 
   Size:        248      73
 
MscI TGG'CCA

Cuts at:      0     112     321 
   Size:        112     209
 
MspA1I CmG'CkG

Cuts at:      0      82     321 
   Size:         82     239
 
NaeI GCC'GGC

Cuts at:      0      88     321 
   Size:         88     233
 
NgoMIV G'CCGG_C

Cuts at:      0      86     321 
   Size:         86     235
 
PstI C_TGCA'G

Cuts at:      0     149     321 
   Size:        149     172
 
SbfI CC_TGCA'GG

Cuts at:      0     149     321 
   Size:        149     172
 
SfcI C'TryA_G

Cuts at:      0     145     321 
   Size:        145     176
 
SmlI C'TyrA_G

Cuts at:      0     232     321 
   Size:        232      89
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0      47      79      92     124     321 
   Size:         47      32      13      32     197

  Fragments arranged by size: 

                 197      47      32      32      13
 

 Enzymes that do cut:

     AloI    AlwNI     ApoI    BpuEI    BsaXI    BseYI    BsrFI   BssHII
   BstAPI     BtgI     BtsI     EaeI     EciI    EcoRI  HindIII     MscI
   MspA1I     NaeI   NgoMIV     PstI     SbfI     SfcI     SmlI     TstI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
   AflIII     AgeI     AhdI     AleI     AlfI     ApaI    ApaLI     AscI
     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI    BanII
     BbeI     BbsI    BbvCI     BcgI    BciVI     BclI    BfrBI     BglI
    BglII     BlpI Bme1580I    BmgBI     BmrI     BmtI     BplI     BpmI
   Bpu10I     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BseRI     BsgI
    BsiEI  BsiHKAI    BsiWI     BsmI    BsmBI Bsp1286I    BspEI    BspHI
    BspMI    BsrBI    BsrDI    BsrGI    BssSI    BstBI   BstEII    BstXI
    BstYI  BstZ17I   Bsu36I    BtgZI     ClaI    CspCI     DraI   DraIII
     DrdI     EagI     EarI  EcoICRI  Eco57MI    EcoNI EcoO109I    EcoRV
     FalI     FseI     FspI    FspAI    HaeII    Hin4I   HincII     HpaI
     KasI     KpnI     MfeI     MluI     MmeI     MslI     NarI     NcoI
     NdeI     NheI     NotI     NruI     NsiI     NspI     PacI     PasI
     PciI    PflMI     PfoI     PmeI     PmlI     PpiI    PpuMI    PshAI
     PsiI   PspOMI    PspXI     PsrI     PvuI    PvuII    RsrII     SacI
    SacII     SalI    SanDI     SapI     ScaI    SexAI     SfiI     SfoI
     SgfI    SgrAI     SmaI    SnaBI     SpeI     SphI     SrfI     SspI
     StuI     StyI     SwaI    TaqII     TatI  Tth111I     XbaI     XcmI
     XhoI     XmaI     XmnI     ZraI