Restriction map for
pFN21AE1413
(Linear) (Six-base) MAPSORT of: KIEE1413.seq Check: 5888 from: 1 to: 1500
KIEE1413.orf
With 182 enzymes: SgfI *
July 15, 2010 18:38 ..
Acc65I G'GTAC_C
Cuts at: 0 1074 1500
Size: 1074 426
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 472 649 1291 1500
Size: 472 177 642 209
Fragments arranged by size:
642 472 209 177
AleI CACnn'nnGTG
Cuts at: 0 706 793 1500
Size: 706 87 707
Fragments arranged by size:
707 706 87
AlwNI CAG_nnn'CTG
Cuts at: 0 376 543 627 1086 1354 1500
Size: 376 167 84 459 268 146
Fragments arranged by size:
459 376 268 167 146 84
ApoI r'AATT_y
Cuts at: 0 1249 1500
Size: 1249 251
AvaI C'yCGr_G
Cuts at: 0 547 1500
Size: 547 953
BamHI G'GATC_C
Cuts at: 0 96 1500
Size: 96 1404
BanI G'GyrC_C
Cuts at: 0 1074 1500
Size: 1074 426
BanII G_rGCy'C
Cuts at: 0 281 782 1500
Size: 281 501 718
Fragments arranged by size:
718 501 281
BbsI GAAGACnn'nnnn_
Cuts at: 0 904 1109 1461 1500
Size: 904 205 352 39
Fragments arranged by size:
904 352 205 39
BglII A'GATC_T
Cuts at: 0 668 1302 1500
Size: 668 634 198
Fragments arranged by size:
668 634 198
Bme1580I G_kGCm'C
Cuts at: 0 968 1500
Size: 968 532
BmrI ACTGGGnnnn_n'
Cuts at: 0 365 1364 1500
Size: 365 999 136
Fragments arranged by size:
999 365 136
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 69 199 598 1177 1500
Size: 69 130 399 579 323
Fragments arranged by size:
579 399 323 130 69
Bpu10I CC'TnA_GC
Cuts at: 0 751 1500
Size: 751 749
BsaI GGTCTCn'nnnn_
Cuts at: 0 484 1500
Size: 484 1016
BsaHI Gr'CG_yC
Cuts at: 0 767 1500
Size: 767 733
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 321 351 1500
Size: 321 30 1149
Fragments arranged by size:
1149 321 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 51 856 1500
Size: 51 805 644
Fragments arranged by size:
805 644 51
BseYI C'CCAG_C
Cuts at: 0 46 320 344 553 1500
Size: 46 274 24 209 947
Fragments arranged by size:
947 274 209 46 24
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1438 1500
Size: 1438 62
BsiHKAI G_wGCw'C
Cuts at: 0 782 1500
Size: 782 718
BsmI GAATG_Cn'
Cuts at: 0 717 1500
Size: 717 783
Bsp1286I G_dGCh'C
Cuts at: 0 281 782 968 1500
Size: 281 501 186 532
Fragments arranged by size:
532 501 281 186
BsrDI GCAATG_nn'
Cuts at: 0 482 1500
Size: 482 1018
BsrFI r'CCGG_y
Cuts at: 0 824 1500
Size: 824 676
BsrGI T'GTAC_A
Cuts at: 0 728 1500
Size: 728 772
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1389 1500
Size: 1389 111
BstYI r'GATC_y
Cuts at: 0 96 668 1302 1500
Size: 96 572 634 198
Fragments arranged by size:
634 572 198 96
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 504 1333 1500
Size: 504 829 167
Fragments arranged by size:
829 504 167
EaeI y'GGCC_r
Cuts at: 0 689 797 1500
Size: 689 108 703
Fragments arranged by size:
703 689 108
EarI CTCTTCn'nnn_
Cuts at: 0 1438 1500
Size: 1438 62
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1368 1500
Size: 1368 132
EcoICRI GAG'CTC
Cuts at: 0 780 1500
Size: 780 720
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 69 199 472 598 649 1177 1291 1500
Size: 69 130 273 126 51 528 114 209
Fragments arranged by size:
528 273 209 130 126 114 69 51
EcoNI CCTnn'n_nnAGG
Cuts at: 0 535 1500
Size: 535 965
EcoO109I rG'GnC_Cy
Cuts at: 0 359 373 540 624 1296 1500
Size: 359 14 167 84 672 204
Fragments arranged by size:
672 359 204 167 84 14
EcoRI G'AATT_C
Cuts at: 0 1249 1500
Size: 1249 251
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 43 75 1213 1245 1500
Size: 43 32 1138 32 255
Fragments arranged by size:
1138 255 43 32 32
KpnI G_GTAC'C
Cuts at: 0 1078 1500
Size: 1078 422
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 324 1500
Size: 324 1176
MscI TGG'CCA
Cuts at: 0 691 799 1500
Size: 691 108 701
Fragments arranged by size:
701 691 108
MslI CAynn'nnrTG
Cuts at: 0 706 793 1204 1387 1500
Size: 706 87 411 183 113
Fragments arranged by size:
706 411 183 113 87
MspA1I CmG'CkG
Cuts at: 0 1038 1500
Size: 1038 462
NaeI GCC'GGC
Cuts at: 0 826 1500
Size: 826 674
NgoMIV G'CCGG_C
Cuts at: 0 824 1500
Size: 824 676
PasI CC'CwG_GG
Cuts at: 0 1493 1500
Size: 1493 7
PfoI T'CCnGG_A
Cuts at: 0 176 1500
Size: 176 1324
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1103 1135 1500
Size: 1103 32 365
Fragments arranged by size:
1103 365 32
PpuMI rG'GwC_Cy
Cuts at: 0 359 373 540 1296 1500
Size: 359 14 167 756 204
Fragments arranged by size:
756 359 204 167 14
PsiI TTA'TAA
Cuts at: 0 958 1500
Size: 958 542
PstI C_TGCA'G
Cuts at: 0 1043 1374 1500
Size: 1043 331 126
Fragments arranged by size:
1043 331 126
PvuII CAG'CTG
Cuts at: 0 1038 1500
Size: 1038 462
SacI G_AGCT'C
Cuts at: 0 782 1500
Size: 782 718
SfcI C'TryA_G
Cuts at: 0 1039 1370 1500
Size: 1039 331 130
Fragments arranged by size:
1039 331 130
StyI C'CwwG_G
Cuts at: 0 599 1025 1500
Size: 599 426 475
Fragments arranged by size:
599 475 426
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 301 322 783 1500
Size: 301 21 461 717
Fragments arranged by size:
717 461 301 21
TatI w'GTAC_w
Cuts at: 0 728 1500
Size: 728 772
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 352 573 1500
Size: 352 221 927
Fragments arranged by size:
927 352 221
XmnI GAAnn'nnTTC
Cuts at: 0 634 935 1500
Size: 634 301 565
Fragments arranged by size:
634 565 301
Enzymes that do cut:
Acc65I AcuI AleI AlwNI ApoI AvaI BamHI BanI
BanII BbsI BglII Bme1580I BmrI BpmI Bpu10I BsaI
BsaHI BsaXI BseRI BseYI BsgI BsiHKAI BsmI Bsp1286I
BsrDI BsrFI BsrGI BstXI BstYI BtgZI EaeI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI Hin4I KpnI
MmeI MscI MslI MspA1I NaeI NgoMIV PasI PfoI
PpiI PpuMI PsiI PstI PvuII SacI SfcI StyI
TaqII TatI XcmI XmnI
Enzymes that do not cut:
AarI AatII AccI AclI AfeI AflII AflIII AgeI
AhdI AlfI AloI ApaI ApaLI AscI AseI AsiSI
AvrII BaeI BbeI BbvCI BcgI BciVI BclI BfrBI
BglI BlpI BmgBI BmtI BplI BpuEI BsaAI BsaBI
BsaWI BsiEI BsiWI BsmBI BspEI BspHI BspMI BsrBI
BssHII BssSI BstAPI BstBI BstEII BstZ17I Bsu36I BtgI
BtsI ClaI CspCI DraI DraIII DrdI EagI EcoRV
FalI FseI FspI FspAI HaeII HincII HindIII HpaI
KasI MfeI MluI NarI NcoI NdeI NheI NotI
NruI NsiI NspI PacI PciI PflMI PmeI PmlI
PshAI PspOMI PspXI PsrI PvuI RsrII SacII SalI
SanDI SapI SbfI ScaI SexAI SfiI SfoI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI
StuI SwaI TstI Tth111I XbaI XhoI XmaI ZraI