(Linear) (Six-base) MAPSORT of: KIEE1410.seq  Check: 3311  from: 1  to: 339



KIEE1410.orf

 With 182 enzymes: SgfI * 

                        July 15, 2010 18:38  ..

BanII G_rGCy'C

Cuts at:      0      58     339 
   Size:         58     281
 
BbsI GAAGACnn'nnnn_

Cuts at:      0      20     339 
   Size:         20     319
 
BmgBI CAC'GTC

Cuts at:      0       9     132     339 
   Size:          9     123     207

  Fragments arranged by size: 

                 207     123       9
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     324     339 
   Size:        324      15
 
Bpu10I CC'TnA_GC

Cuts at:      0      57     339 
   Size:         57     282
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      79     339 
   Size:         79     260
 
BsaAI yAC'GTr

Cuts at:      0     146     339 
   Size:        146     193
 
BseYI C'CCAG_C

Cuts at:      0     125     339 
   Size:        125     214
 
BsiHKAI G_wGCw'C

Cuts at:      0      58     339 
   Size:         58     281
 
Bsp1286I G_dGCh'C

Cuts at:      0      58     339 
   Size:         58     281
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0      37     339 
   Size:         37     302
 
DrdI GACnn_nn'nnGTC

Cuts at:      0     319     339 
   Size:        319      20
 
EaeI y'GGCC_r

Cuts at:      0       2     113     339 
   Size:          2     111     226

  Fragments arranged by size: 

                 226     111       2
 
EcoICRI GAG'CTC

Cuts at:      0      56     339 
   Size:         56     283
 
MscI TGG'CCA

Cuts at:      0     115     339 
   Size:        115     224
 
NspI r_CATG'y

Cuts at:      0     267     339 
   Size:        267      72
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     171     339 
   Size:        171     168
 
PfoI T'CCnGG_A

Cuts at:      0      49     339 
   Size:         49     290
 
PstI C_TGCA'G

Cuts at:      0     160     339 
   Size:        160     179
 
SacI G_AGCT'C

Cuts at:      0      58     339 
   Size:         58     281
 
SfcI C'TryA_G

Cuts at:      0     156     339 
   Size:        156     183
 
SmlI C'TyrA_G

Cuts at:      0      94     339 
   Size:         94     245
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     264     306     339 
   Size:        264      42      33

  Fragments arranged by size: 

                 264      42      33
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     121     153     339 
   Size:        121      32     186

  Fragments arranged by size: 

                 186     121      32
 

 Enzymes that do cut:

    BanII     BbsI    BmgBI     BmrI   Bpu10I    BpuEI    BsaAI    BseYI
  BsiHKAI Bsp1286I    BspMI     DrdI     EaeI  EcoICRI     MscI     NspI
    PflMI     PfoI     PstI     SacI     SfcI     SmlI    TaqII     TstI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
   AflIII     AgeI     AhdI     AleI     AlfI     AloI    AlwNI     ApaI
    ApaLI     ApoI     AscI     AseI    AsiSI     AvaI    AvrII     BaeI
    BamHI     BanI     BbeI    BbvCI     BcgI    BciVI     BclI    BfrBI
     BglI    BglII     BlpI Bme1580I     BmtI     BplI     BpmI     BsaI
    BsaBI    BsaHI    BsaWI    BsaXI    BseRI     BsgI    BsiEI    BsiWI
     BsmI    BsmBI    BspEI    BspHI    BsrBI    BsrDI    BsrFI    BsrGI
   BssHII    BssSI   BstAPI    BstBI   BstEII    BstXI    BstYI  BstZ17I
   Bsu36I     BtgI    BtgZI     BtsI     ClaI    CspCI     DraI   DraIII
     EagI     EarI     EciI  Eco57MI    EcoNI EcoO109I    EcoRI    EcoRV
     FalI     FseI     FspI    FspAI    HaeII    Hin4I   HincII  HindIII
     HpaI     KasI     KpnI     MfeI     MluI     MmeI     MslI   MspA1I
     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI     NotI     NruI
     NsiI     PacI     PasI     PciI     PmeI     PmlI     PpiI    PpuMI
    PshAI     PsiI   PspOMI    PspXI     PsrI     PvuI    PvuII    RsrII
    SacII     SalI    SanDI     SapI     SbfI     ScaI    SexAI     SfiI
     SfoI     SgfI    SgrAI     SmaI    SnaBI     SpeI     SphI     SrfI
     SspI     StuI     StyI     SwaI     TatI  Tth111I     XbaI     XcmI
     XhoI     XmaI     XmnI     ZraI