(Linear) (Six-base) MAPSORT of: KIEE1405.seq  Check: 9421  from: 1  to: 300



KIEE1405.orf

 With 182 enzymes: SgfI * 

                        July 15, 2010 18:38  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     154     300 
   Size:        154     146
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0      44      76     300 
   Size:         44      32     224

  Fragments arranged by size: 

                 224      44      32
 
AlwNI CAG_nnn'CTG

Cuts at:      0      19     300 
   Size:         19     281
 
BanII G_rGCy'C

Cuts at:      0     163     300 
   Size:        163     137
 
BclI T'GATC_A

Cuts at:      0     224     300 
   Size:        224      76
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     279     300 
   Size:        279      21
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0      44      74     300 
   Size:         44      30     226

  Fragments arranged by size: 

                 226      44      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      19     300 
   Size:         19     281
 
Bsp1286I G_dGCh'C

Cuts at:      0     163     300 
   Size:        163     137
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     154     300 
   Size:        154     146
 
EcoO109I rG'GnC_Cy

Cuts at:      0     138     300 
   Size:        138     162
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     207     300 
   Size:        207      93
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     169     201     300 
   Size:        169      32      99

  Fragments arranged by size: 

                 169      99      32
 
HincII GTy'rAC

Cuts at:      0     252     300 
   Size:        252      48
 
HpaI GTT'AAC

Cuts at:      0     252     300 
   Size:        252      48
 
MspA1I CmG'CkG

Cuts at:      0     213     300 
   Size:        213      87
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0      44      76     300 
   Size:         44      32     224

  Fragments arranged by size: 

                 224      44      32
 
PpuMI rG'GwC_Cy

Cuts at:      0     138     300 
   Size:        138     162
 
PstI C_TGCA'G

Cuts at:      0      43     300 
   Size:         43     257
 
PvuII CAG'CTG

Cuts at:      0     213     300 
   Size:        213      87
 
ScaI AGT'ACT

Cuts at:      0     109     300 
   Size:        109     191
 
SfcI C'TryA_G

Cuts at:      0      39     300 
   Size:         39     261
 
SmlI C'TyrA_G

Cuts at:      0     258     300 
   Size:        258      42
 
TatI w'GTAC_w

Cuts at:      0     107     300 
   Size:        107     193
 

 Enzymes that do cut:

     AcuI     AloI    AlwNI    BanII     BclI    BpuEI    BsaXI    BseRI
 Bsp1286I  Eco57MI EcoO109I     FalI    Hin4I   HincII     HpaI   MspA1I
     PpiI    PpuMI     PstI    PvuII     ScaI     SfcI     SmlI     TatI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI     ApaI    ApaLI     ApoI     AscI
     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI     BbeI
     BbsI    BbvCI     BcgI    BciVI    BfrBI     BglI    BglII     BlpI
 Bme1580I    BmgBI     BmrI     BmtI     BplI     BpmI   Bpu10I     BsaI
    BsaAI    BsaBI    BsaHI    BsaWI    BseYI     BsgI    BsiEI  BsiHKAI
    BsiWI     BsmI    BsmBI    BspEI    BspHI    BspMI    BsrBI    BsrDI
    BsrFI    BsrGI   BssHII    BssSI   BstAPI    BstBI   BstEII    BstXI
    BstYI  BstZ17I   Bsu36I     BtgI    BtgZI     BtsI     ClaI    CspCI
     DraI   DraIII     DrdI     EaeI     EagI     EarI     EciI  EcoICRI
    EcoNI    EcoRI    EcoRV     FseI     FspI    FspAI    HaeII  HindIII
     KasI     KpnI     MfeI     MluI     MmeI     MscI     MslI     NaeI
     NarI     NcoI     NdeI   NgoMIV     NheI     NotI     NruI     NsiI
     NspI     PacI     PasI     PciI    PflMI     PfoI     PmeI     PmlI
    PshAI     PsiI   PspOMI    PspXI     PsrI     PvuI    RsrII     SacI
    SacII     SalI    SanDI     SapI     SbfI    SexAI     SfiI     SfoI
     SgfI    SgrAI     SmaI    SnaBI     SpeI     SphI     SrfI     SspI
     StuI     StyI     SwaI    TaqII     TstI  Tth111I     XbaI     XcmI
     XhoI     XmaI     XmnI     ZraI