Restriction map for
pFN21AE1404
(Linear) (Six-base) MAPSORT of: KIEE1404.seq Check: 5863 from: 1 to: 1014
KIEE1404.orf
With 182 enzymes: SgfI *
July 15, 2010 18:38 ..
AccI GT'mk_AC
Cuts at: 0 984 1014
Size: 984 30
Acc65I G'GTAC_C
Cuts at: 0 43 1014
Size: 43 971
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 373 1014
Size: 373 641
AflIII A'CryG_T
Cuts at: 0 987 1014
Size: 987 27
AlwNI CAG_nnn'CTG
Cuts at: 0 391 699 1014
Size: 391 308 315
Fragments arranged by size:
391 315 308
ApaI G_GGCC'C
Cuts at: 0 400 1014
Size: 400 614
ApaLI G'TGCA_C
Cuts at: 0 110 1014
Size: 110 904
ApoI r'AATT_y
Cuts at: 0 744 1014
Size: 744 270
BanI G'GyrC_C
Cuts at: 0 43 189 1014
Size: 43 146 825
Fragments arranged by size:
825 146 43
BanII G_rGCy'C
Cuts at: 0 400 460 1014
Size: 400 60 554
Fragments arranged by size:
554 400 60
BbsI GAAGACnn'nnnn_
Cuts at: 0 952 1014
Size: 952 62
BfrBI ATG'CAT
Cuts at: 0 688 1014
Size: 688 326
Bme1580I G_kGCm'C
Cuts at: 0 114 400 1014
Size: 114 286 614
Fragments arranged by size:
614 286 114
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 436 481 862 1014
Size: 436 45 381 152
Fragments arranged by size:
436 381 152 45
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 591 877 1014
Size: 591 286 137
Fragments arranged by size:
591 286 137
BsaI GGTCTCn'nnnn_
Cuts at: 0 610 1014
Size: 610 404
BsaAI yAC'GTr
Cuts at: 0 988 1014
Size: 988 26
BsaBI GATnn'nnATC
Cuts at: 0 938 1014
Size: 938 76
BsaWI w'CCGG_w
Cuts at: 0 966 1014
Size: 966 48
BseYI C'CCAG_C
Cuts at: 0 453 1014
Size: 453 561
BsiHKAI G_wGCw'C
Cuts at: 0 114 1014
Size: 114 900
Bsp1286I G_dGCh'C
Cuts at: 0 114 400 460 1014
Size: 114 286 60 554
Fragments arranged by size:
554 286 114 60
BsrDI GCAATG_nn'
Cuts at: 0 688 1014
Size: 688 326
BstAPI GCAn_nnn'nTGC
Cuts at: 0 699 1014
Size: 699 315
BstXI CCAn_nnnn'nTGG
Cuts at: 0 414 1014
Size: 414 600
BstYI r'GATC_y
Cuts at: 0 420 777 887 1014
Size: 420 357 110 127
Fragments arranged by size:
420 357 127 110
Bsu36I CC'TnA_GG
Cuts at: 0 217 1014
Size: 217 797
BtsI GCAGTG_nn'
Cuts at: 0 283 1014
Size: 283 731
DraI TTT'AAA
Cuts at: 0 540 1014
Size: 540 474
EaeI y'GGCC_r
Cuts at: 0 51 1014
Size: 51 963
EarI CTCTTCn'nnn_
Cuts at: 0 28 623 1014
Size: 28 595 391
Fragments arranged by size:
595 391 28
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 373 436 481 862 1014
Size: 373 63 45 381 152
Fragments arranged by size:
381 373 152 63 45
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 564 596 1014
Size: 564 32 418
Fragments arranged by size:
564 418 32
HindIII A'AGCT_T
Cuts at: 0 793 1014
Size: 793 221
KpnI G_GTAC'C
Cuts at: 0 47 1014
Size: 47 967
MscI TGG'CCA
Cuts at: 0 53 1014
Size: 53 961
MslI CAynn'nnrTG
Cuts at: 0 117 242 1014
Size: 117 125 772
Fragments arranged by size:
772 125 117
MspA1I CmG'CkG
Cuts at: 0 532 1014
Size: 532 482
NsiI A_TGCA'T
Cuts at: 0 690 1014
Size: 690 324
PfoI T'CCnGG_A
Cuts at: 0 195 1014
Size: 195 819
PspOMI G'GGCC_C
Cuts at: 0 396 1014
Size: 396 618
PstI C_TGCA'G
Cuts at: 0 278 428 1014
Size: 278 150 586
Fragments arranged by size:
586 278 150
PvuII CAG'CTG
Cuts at: 0 532 1014
Size: 532 482
ScaI AGT'ACT
Cuts at: 0 58 1014
Size: 58 956
SfcI C'TryA_G
Cuts at: 0 274 424 1014
Size: 274 150 590
Fragments arranged by size:
590 274 150
SmlI C'TyrA_G
Cuts at: 0 570 856 1014
Size: 570 286 158
Fragments arranged by size:
570 286 158
StuI AGG'CCT
Cuts at: 0 253 1014
Size: 253 761
TatI w'GTAC_w
Cuts at: 0 56 152 1014
Size: 56 96 862
Fragments arranged by size:
862 96 56
Tth111I GACn'n_nGTC
Cuts at: 0 387 1014
Size: 387 627
XbaI T'CTAG_A
Cuts at: 0 288 303 1014
Size: 288 15 711
Fragments arranged by size:
711 288 15
Enzymes that do cut:
AccI Acc65I AcuI AflIII AlwNI ApaI ApaLI ApoI
BanI BanII BbsI BfrBI Bme1580I BpmI BpuEI BsaI
BsaAI BsaBI BsaWI BseYI BsiHKAI Bsp1286I BsrDI BstAPI
BstXI BstYI Bsu36I BtsI DraI EaeI EarI Eco57MI
Hin4I HindIII KpnI MscI MslI MspA1I NsiI PfoI
PspOMI PstI PvuII ScaI SfcI SmlI StuI TatI
Tth111I XbaI
Enzymes that do not cut:
AarI AatII AclI AfeI AflII AgeI AhdI AleI
AlfI AloI AscI AseI AsiSI AvaI AvrII BaeI
BamHI BbeI BbvCI BcgI BciVI BclI BglI BglII
BlpI BmgBI BmrI BmtI BplI Bpu10I BsaHI BsaXI
BseRI BsgI BsiEI BsiWI BsmI BsmBI BspEI BspHI
BspMI BsrBI BsrFI BsrGI BssHII BssSI BstBI BstEII
BstZ17I BtgI BtgZI ClaI CspCI DraIII DrdI EagI
EciI EcoICRI EcoNI EcoO109I EcoRI EcoRV FalI FseI
FspI FspAI HaeII HincII HpaI KasI MfeI MluI
MmeI NaeI NarI NcoI NdeI NgoMIV NheI NotI
NruI NspI PacI PasI PciI PflMI PmeI PmlI
PpiI PpuMI PshAI PsiI PspXI PsrI PvuI RsrII
SacI SacII SalI SanDI SapI SbfI SexAI SfiI
SfoI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI
SspI StyI SwaI TaqII TstI XcmI XhoI XmaI
XmnI ZraI