Restriction map for
pFN21AE1402
(Linear) (Six-base) MAPSORT of: KIEE1402.seq Check: 2496 from: 1 to: 1797
KIEE1402.orf
With 182 enzymes: SgfI *
July 15, 2010 18:38 ..
AccI GT'mk_AC
Cuts at: 0 680 1797
Size: 680 1117
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 964 1708 1797
Size: 964 744 89
Fragments arranged by size:
964 744 89
AleI CACnn'nnGTG
Cuts at: 0 91 926 1797
Size: 91 835 871
Fragments arranged by size:
871 835 91
AlwNI CAG_nnn'CTG
Cuts at: 0 525 765 1050 1797
Size: 525 240 285 747
Fragments arranged by size:
747 525 285 240
ApoI r'AATT_y
Cuts at: 0 234 1075 1797
Size: 234 841 722
Fragments arranged by size:
841 722 234
AvaI C'yCGr_G
Cuts at: 0 824 1361 1797
Size: 824 537 436
Fragments arranged by size:
824 537 436
AvrII C'CTAG_G
Cuts at: 0 1599 1797
Size: 1599 198
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 739 772 1797
Size: 739 33 1025
Fragments arranged by size:
1025 739 33
BanI G'GyrC_C
Cuts at: 0 81 574 801 1157 1797
Size: 81 493 227 356 640
Fragments arranged by size:
640 493 356 227 81
BanII G_rGCy'C
Cuts at: 0 890 1192 1439 1578 1796 1797
Size: 890 302 247 139 218 1
Fragments arranged by size:
890 302 247 218 139 1
BbeI G_GCGC'C
Cuts at: 0 85 805 1797
Size: 85 720 992
Fragments arranged by size:
992 720 85
BlpI GC'TnA_GC
Cuts at: 0 950 1797
Size: 950 847
BmrI ACTGGGnnnn_n'
Cuts at: 0 365 1797
Size: 365 1432
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 346 438 1797
Size: 346 92 1359
Fragments arranged by size:
1359 346 92
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 730 1797
Size: 730 1067
Bpu10I CC'TnA_GC
Cuts at: 0 544 1797
Size: 544 1253
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 736 1573 1797
Size: 736 837 224
Fragments arranged by size:
837 736 224
BsaI GGTCTCn'nnnn_
Cuts at: 0 1006 1600 1797
Size: 1006 594 197
Fragments arranged by size:
1006 594 197
BsaAI yAC'GTr
Cuts at: 0 916 1041 1797
Size: 916 125 756
Fragments arranged by size:
916 756 125
BsaBI GATnn'nnATC
Cuts at: 0 1027 1797
Size: 1027 770
BsaHI Gr'CG_yC
Cuts at: 0 82 802 1797
Size: 82 720 995
Fragments arranged by size:
995 720 82
BsaWI w'CCGG_w
Cuts at: 0 20 230 1620 1797
Size: 20 210 1390 177
Fragments arranged by size:
1390 210 177 20
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 703 733 1462 1492 1797
Size: 703 30 729 30 305
Fragments arranged by size:
729 703 305 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1048 1797
Size: 1048 749
BseYI C'CCAG_C
Cuts at: 0 247 410 518 1797
Size: 247 163 108 1279
Fragments arranged by size:
1279 247 163 108
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 156 186 1063 1797
Size: 156 30 877 734
Fragments arranged by size:
877 734 156 30
BsiHKAI G_wGCw'C
Cuts at: 0 923 956 1439 1642 1796 1797
Size: 923 33 483 203 154 1
Fragments arranged by size:
923 483 203 154 33 1
Bsp1286I G_dGCh'C
Cuts at: 0 890 923 956 1192 1439 1578 1642 1796
Size: 890 33 33 236 247 139 64 154
Cuts at: 1796 1797
Size: 1
Fragments arranged by size:
890 247 236 154 139 64 33 33
1
BspEI T'CCGG_A
Cuts at: 0 230 1620 1797
Size: 230 1390 177
Fragments arranged by size:
1390 230 177
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 947 1797
Size: 947 850
BsrBI CCG'CTC
Cuts at: 0 54 1797
Size: 54 1743
BsrDI GCAATG_nn'
Cuts at: 0 1124 1797
Size: 1124 673
BstXI CCAn_nnnn'nTGG
Cuts at: 0 483 666 697 1239 1288 1797
Size: 483 183 31 542 49 509
Fragments arranged by size:
542 509 483 183 49 31
BtgI C'CryG_G
Cuts at: 0 482 672 1130 1797
Size: 482 190 458 667
Fragments arranged by size:
667 482 458 190
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 983 1797
Size: 983 814
BtsI GCAGTG_nn'
Cuts at: 0 238 1797
Size: 238 1559
EaeI y'GGCC_r
Cuts at: 0 687 1290 1679 1797
Size: 687 603 389 118
Fragments arranged by size:
687 603 389 118
EarI CTCTTCn'nnn_
Cuts at: 0 791 1797
Size: 791 1006
EcoICRI GAG'CTC
Cuts at: 0 1437 1794 1797
Size: 1437 357 3
Fragments arranged by size:
1437 357 3
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 730 964 1708 1797
Size: 730 234 744 89
Fragments arranged by size:
744 730 234 89
EcoNI CCTnn'n_nnAGG
Cuts at: 0 943 1797
Size: 943 854
EcoO109I rG'GnC_Cy
Cuts at: 0 646 1257 1797
Size: 646 611 540
Fragments arranged by size:
646 611 540
EcoRI G'AATT_C
Cuts at: 0 234 1797
Size: 234 1563
HaeII r_GCGC'y
Cuts at: 0 85 288 805 1797
Size: 85 203 517 992
Fragments arranged by size:
992 517 203 85
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 26 1258 1290 1560 1592 1768 1797
Size: 26 1232 32 270 32 176 29
Fragments arranged by size:
1232 270 176 32 32 29 26
KasI G'GCGC_C
Cuts at: 0 81 801 1797
Size: 81 720 996
Fragments arranged by size:
996 720 81
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 383 449 1448 1478 1797
Size: 383 66 999 30 319
Fragments arranged by size:
999 383 319 66 30
MslI CAynn'nnrTG
Cuts at: 0 91 481 695 926 1129 1231 1237 1797
Size: 91 390 214 231 203 102 6 560
Fragments arranged by size:
560 390 231 214 203 102 91 6
MspA1I CmG'CkG
Cuts at: 0 410 1050 1071 1797
Size: 410 640 21 726
Fragments arranged by size:
726 640 410 21
NarI GG'CG_CC
Cuts at: 0 82 802 1797
Size: 82 720 995
Fragments arranged by size:
995 720 82
NcoI C'CATG_G
Cuts at: 0 482 672 1130 1797
Size: 482 190 458 667
Fragments arranged by size:
667 482 458 190
NspI r_CATG'y
Cuts at: 0 1336 1797
Size: 1336 461
PasI CC'CwG_GG
Cuts at: 0 76 1797
Size: 76 1721
PflMI CCAn_nnn'nTGG
Cuts at: 0 525 765 908 1797
Size: 525 240 143 889
Fragments arranged by size:
889 525 240 143
PfoI T'CCnGG_A
Cuts at: 0 1430 1797
Size: 1430 367
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1078 1110 1797
Size: 1078 32 687
Fragments arranged by size:
1078 687 32
PpuMI rG'GwC_Cy
Cuts at: 0 646 1797
Size: 646 1151
PstI C_TGCA'G
Cuts at: 0 1070 1379 1797
Size: 1070 309 418
Fragments arranged by size:
1070 418 309
PvuII CAG'CTG
Cuts at: 0 410 1050 1071 1797
Size: 410 640 21 726
Fragments arranged by size:
726 640 410 21
SacI G_AGCT'C
Cuts at: 0 1439 1796 1797
Size: 1439 357 1
Fragments arranged by size:
1439 357 1
ScaI AGT'ACT
Cuts at: 0 1627 1797
Size: 1627 170
SexAI A'CCwGG_T
Cuts at: 0 758 1797
Size: 758 1039
SfcI C'TryA_G
Cuts at: 0 1066 1375 1797
Size: 1066 309 422
Fragments arranged by size:
1066 422 309
SfoI GGC'GCC
Cuts at: 0 83 803 1797
Size: 83 720 994
Fragments arranged by size:
994 720 83
SmlI C'TyrA_G
Cuts at: 0 751 1588 1797
Size: 751 837 209
Fragments arranged by size:
837 751 209
StuI AGG'CCT
Cuts at: 0 664 1797
Size: 664 1133
StyI C'CwwG_G
Cuts at: 0 482 570 659 665 672 1130 1599 1797
Size: 482 88 89 6 7 458 469 198
Fragments arranged by size:
482 469 458 198 89 88 7 6
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 277 495 628 1797
Size: 277 218 133 1169
Fragments arranged by size:
1169 277 218 133
TatI w'GTAC_w
Cuts at: 0 1199 1625 1797
Size: 1199 426 172
Fragments arranged by size:
1199 426 172
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 766 868 1797
Size: 766 102 929
Fragments arranged by size:
929 766 102
Enzymes that do cut:
AccI AcuI AleI AlwNI ApoI AvaI AvrII BaeI
BanI BanII BbeI BlpI BmrI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiHKAI Bsp1286I BspEI BspMI BsrBI BsrDI
BstXI BtgI BtgZI BtsI EaeI EarI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI HaeII Hin4I KasI MmeI MslI
MspA1I NarI NcoI NspI PasI PflMI PfoI PpiI
PpuMI PstI PvuII SacI ScaI SexAI SfcI SfoI
SmlI StuI StyI TaqII TatI XcmI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AflIII AgeI
AhdI AlfI AloI ApaI ApaLI AscI AseI AsiSI
BamHI BbsI BbvCI BcgI BciVI BclI BfrBI BglI
BglII Bme1580I BmgBI BmtI BsiEI BsiWI BsmI BsmBI
BspHI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstYI BstZ17I Bsu36I ClaI CspCI DraI DraIII DrdI
EagI EciI EcoRV FalI FseI FspI FspAI HincII
HindIII HpaI KpnI MfeI MluI MscI NaeI NdeI
NgoMIV NheI NotI NruI NsiI PacI PciI PmeI
PmlI PshAI PsiI PspOMI PspXI PsrI PvuI RsrII
SacII SalI SanDI SapI SbfI SfiI SgfI SgrAI
SmaI SnaBI SpeI SphI SrfI SspI SwaI TstI
Tth111I XbaI XhoI XmaI XmnI ZraI