(Linear) (Six-base) MAPSORT of: KIEE1396.seq  Check: 646  from: 1  to: 201



KIEE1396.orf

 With 182 enzymes: SgfI * 

                        July 15, 2010 18:38  ..

AflIII A'CryG_T

Cuts at:      0     169     201 
   Size:        169      32
 
BamHI G'GATC_C

Cuts at:      0       5     201 
   Size:          5     196
 
BanI G'GyrC_C

Cuts at:      0      45     201 
   Size:         45     156
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     167     201 
   Size:        167      34
 
BlpI GC'TnA_GC

Cuts at:      0     127     201 
   Size:        127      74
 
BsiEI CG_ry'CG

Cuts at:      0     114     201 
   Size:        114      87
 
BsiHKAI G_wGCw'C

Cuts at:      0     128     166     201 
   Size:        128      38      35

  Fragments arranged by size: 

                 128      38      35
 
Bsp1286I G_dGCh'C

Cuts at:      0     128     166     201 
   Size:        128      38      35

  Fragments arranged by size: 

                 128      38      35
 
BsrFI r'CCGG_y

Cuts at:      0     113     201 
   Size:        113      88
 
BstYI r'GATC_y

Cuts at:      0       5     201 
   Size:          5     196
 
EaeI y'GGCC_r

Cuts at:      0     111     174     201 
   Size:        111      63      27

  Fragments arranged by size: 

                 111      63      27
 
EagI C'GGCC_G

Cuts at:      0     111     201 
   Size:        111      90
 
EarI CTCTTCn'nnn_

Cuts at:      0      37     201 
   Size:         37     164
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0      53     139     201 
   Size:         53      86      62

  Fragments arranged by size: 

                  86      62      53
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0      29     201 
   Size:         29     172
 
MluI A'CGCG_T

Cuts at:      0     169     201 
   Size:        169      32
 
MspA1I CmG'CkG

Cuts at:      0     131     201 
   Size:        131      70
 
PflMI CCAn_nnn'nTGG

Cuts at:      0      61     201 
   Size:         61     140
 
PvuII CAG'CTG

Cuts at:      0     131     201 
   Size:        131      70
 
SapI GCTCTTCn'nnn_

Cuts at:      0      37     201 
   Size:         37     164
 
SspI AAT'ATT

Cuts at:      0      91     201 
   Size:         91     110
 

 Enzymes that do cut:

   AflIII    BamHI     BanI     BcgI     BlpI    BsiEI  BsiHKAI Bsp1286I
    BsrFI    BstYI     EaeI     EagI     EarI    EcoNI    Hin4I     MluI
   MspA1I    PflMI    PvuII     SapI     SspI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
     AgeI     AhdI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI
     ApoI     AscI     AseI    AsiSI     AvaI    AvrII     BaeI    BanII
     BbeI     BbsI    BbvCI    BciVI     BclI    BfrBI     BglI    BglII
 Bme1580I    BmgBI     BmrI     BmtI     BplI     BpmI   Bpu10I    BpuEI
     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI    BseRI    BseYI
     BsgI    BsiWI     BsmI    BsmBI    BspEI    BspHI    BspMI    BsrBI
    BsrDI    BsrGI   BssHII    BssSI   BstAPI    BstBI   BstEII    BstXI
  BstZ17I   Bsu36I     BtgI    BtgZI     BtsI     ClaI    CspCI     DraI
   DraIII     DrdI     EciI  EcoICRI  Eco57MI EcoO109I    EcoRI    EcoRV
     FalI     FseI     FspI    FspAI    HaeII   HincII  HindIII     HpaI
     KasI     KpnI     MfeI     MmeI     MscI     MslI     NaeI     NarI
     NcoI     NdeI   NgoMIV     NheI     NotI     NruI     NsiI     NspI
     PacI     PasI     PciI     PfoI     PmeI     PmlI     PpiI    PpuMI
    PshAI     PsiI   PspOMI    PspXI     PsrI     PstI     PvuI    RsrII
     SacI    SacII     SalI    SanDI     SbfI     ScaI    SexAI     SfcI
     SfiI     SfoI     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI
     SphI     SrfI     StuI     StyI     SwaI    TaqII     TatI     TstI
  Tth111I     XbaI     XcmI     XhoI     XmaI     XmnI     ZraI