Restriction map for
pFN21AE1395
(Linear) (Six-base) MAPSORT of: KIEE1395.seq Check: 9205 from: 1 to: 1080
KIEE1395.orf
With 182 enzymes: SgfI *
July 15, 2010 18:38 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 663 1080
Size: 663 417
AlwNI CAG_nnn'CTG
Cuts at: 0 377 1080
Size: 377 703
ApaLI G'TGCA_C
Cuts at: 0 1033 1080
Size: 1033 47
ApoI r'AATT_y
Cuts at: 0 496 816 1080
Size: 496 320 264
Fragments arranged by size:
496 320 264
AvaI C'yCGr_G
Cuts at: 0 61 893 1080
Size: 61 832 187
Fragments arranged by size:
832 187 61
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 816 849 1080
Size: 816 33 231
Fragments arranged by size:
816 231 33
BanII G_rGCy'C
Cuts at: 0 552 1080
Size: 552 528
BbvCI CC'TCA_GC
Cuts at: 0 345 1080
Size: 345 735
BglI GCCn_nnn'nGGC
Cuts at: 0 290 1080
Size: 290 790
Bme1580I G_kGCm'C
Cuts at: 0 1037 1080
Size: 1037 43
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 87 870 1080
Size: 87 783 210
Fragments arranged by size:
783 210 87
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 93 371 723 806 1080
Size: 93 278 352 83 274
Fragments arranged by size:
352 278 274 93 83
Bpu10I CC'TnA_GC
Cuts at: 0 345 1080
Size: 345 735
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 928 958 1080
Size: 928 30 122
Fragments arranged by size:
928 122 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 96 333 1080
Size: 96 237 747
Fragments arranged by size:
747 237 96
BsiHKAI G_wGCw'C
Cuts at: 0 1037 1080
Size: 1037 43
BsmI GAATG_Cn'
Cuts at: 0 229 1080
Size: 229 851
Bsp1286I G_dGCh'C
Cuts at: 0 552 1037 1080
Size: 552 485 43
Fragments arranged by size:
552 485 43
BspHI T'CATG_A
Cuts at: 0 734 1080
Size: 734 346
BsrBI CCG'CTC
Cuts at: 0 635 1080
Size: 635 445
BssSI C'ACGA_G
Cuts at: 0 802 1080
Size: 802 278
BstXI CCAn_nnnn'nTGG
Cuts at: 0 729 1080
Size: 729 351
BtgI C'CryG_G
Cuts at: 0 624 668 1080
Size: 624 44 412
Fragments arranged by size:
624 412 44
BtsI GCAGTG_nn'
Cuts at: 0 477 1080
Size: 477 603
DraIII CAC_nnn'GTG
Cuts at: 0 630 1080
Size: 630 450
EaeI y'GGCC_r
Cuts at: 0 206 665 671 1080
Size: 206 459 6 409
Fragments arranged by size:
459 409 206 6
EarI CTCTTCn'nnn_
Cuts at: 0 78 1080
Size: 78 1002
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 229 1080
Size: 229 851
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 93 371 663 723 806 1080
Size: 93 278 292 60 83 274
Fragments arranged by size:
292 278 274 93 83 60
EcoO109I rG'GnC_Cy
Cuts at: 0 297 374 1047 1080
Size: 297 77 673 33
Fragments arranged by size:
673 297 77 33
EcoRI G'AATT_C
Cuts at: 0 816 1080
Size: 816 264
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 618 1080
Size: 618 462
HaeII r_GCGC'y
Cuts at: 0 434 1080
Size: 434 646
HincII GTy'rAC
Cuts at: 0 69 1080
Size: 69 1011
MscI TGG'CCA
Cuts at: 0 667 1080
Size: 667 413
MslI CAynn'nnrTG
Cuts at: 0 176 727 958 1080
Size: 176 551 231 122
Fragments arranged by size:
551 231 176 122
MspA1I CmG'CkG
Cuts at: 0 153 159 251 349 908 1080
Size: 153 6 92 98 559 172
Fragments arranged by size:
559 172 153 98 92 6
NcoI C'CATG_G
Cuts at: 0 668 1080
Size: 668 412
NspI r_CATG'y
Cuts at: 0 659 1043 1080
Size: 659 384 37
Fragments arranged by size:
659 384 37
PasI CC'CwG_GG
Cuts at: 0 858 1080
Size: 858 222
PflMI CCAn_nnn'nTGG
Cuts at: 0 204 1080
Size: 204 876
PpuMI rG'GwC_Cy
Cuts at: 0 297 1080
Size: 297 783
PstI C_TGCA'G
Cuts at: 0 472 1080
Size: 472 608
PvuII CAG'CTG
Cuts at: 0 159 349 908 1080
Size: 159 190 559 172
Fragments arranged by size:
559 190 172 159
SfcI C'TryA_G
Cuts at: 0 95 378 468 1080
Size: 95 283 90 612
Fragments arranged by size:
612 283 95 90
SmaI CCC'GGG
Cuts at: 0 895 1080
Size: 895 185
StuI AGG'CCT
Cuts at: 0 137 1080
Size: 137 943
StyI C'CwwG_G
Cuts at: 0 384 434 668 1080
Size: 384 50 234 412
Fragments arranged by size:
412 384 234 50
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 857 962 1080
Size: 857 105 118
Fragments arranged by size:
857 118 105
TatI w'GTAC_w
Cuts at: 0 643 710 1080
Size: 643 67 370
Fragments arranged by size:
643 370 67
XmaI C'CCGG_G
Cuts at: 0 893 1080
Size: 893 187
Enzymes that do cut:
AcuI AlwNI ApaLI ApoI AvaI BaeI BanII BbvCI
BglI Bme1580I BplI BpmI Bpu10I BsaXI BseRI BsiHKAI
BsmI Bsp1286I BspHI BsrBI BssSI BstXI BtgI BtsI
DraIII EaeI EarI EciI Eco57MI EcoO109I EcoRI FalI
HaeII HincII MscI MslI MspA1I NcoI NspI PasI
PflMI PpuMI PstI PvuII SfcI SmaI StuI StyI
TaqII TatI XmaI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI ApaI AscI AseI
AsiSI AvrII BamHI BanI BbeI BbsI BcgI BciVI
BclI BfrBI BglII BlpI BmgBI BmrI BmtI BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BseYI BsgI BsiEI
BsiWI BsmBI BspEI BspMI BsrDI BsrFI BsrGI BssHII
BstAPI BstBI BstEII BstYI BstZ17I Bsu36I BtgZI ClaI
CspCI DraI DrdI EagI EcoICRI EcoNI EcoRV FseI
FspI FspAI Hin4I HindIII HpaI KasI KpnI MfeI
MluI MmeI NaeI NarI NdeI NgoMIV NheI NotI
NruI NsiI PacI PciI PfoI PmeI PmlI PpiI
PshAI PsiI PspOMI PspXI PsrI PvuI RsrII SacI
SacII SalI SanDI SapI SbfI ScaI SexAI SfiI
SfoI SgfI SgrAI SmlI SnaBI SpeI SphI SrfI
SspI SwaI TstI Tth111I XbaI XcmI XhoI XmnI
ZraI