(Linear) (Six-base) MAPSORT of: KIEE1393.seq  Check: 2989  from: 1  to: 279



KIEE1393.orf

 With 182 enzymes: SgfI * 

                        July 15, 2010 18:38  ..

AatII G_ACGT'C

Cuts at:      0      44     158     279 
   Size:         44     114     121

  Fragments arranged by size: 

                 121     114      44
 
AccI GT'mk_AC

Cuts at:      0      44      86     279 
   Size:         44      42     193

  Fragments arranged by size: 

                 193      44      42
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     121     279 
   Size:        121     158
 
AhdI GACnn_n'nnGTC

Cuts at:      0     122     279 
   Size:        122     157
 
ApoI r'AATT_y

Cuts at:      0      72     279 
   Size:         72     207
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      34     279 
   Size:         34     245
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     109     279 
   Size:        109     170
 
BsaHI Gr'CG_yC

Cuts at:      0      41     155     279 
   Size:         41     114     124

  Fragments arranged by size: 

                 124     114      41
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0      40      70     279 
   Size:         40      30     209

  Fragments arranged by size: 

                 209      40      30
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     170     212     279 
   Size:        170      42      67

  Fragments arranged by size: 

                 170      67      42
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      34     121     279 
   Size:         34      87     158

  Fragments arranged by size: 

                 158      87      34
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0      64     279 
   Size:         64     215
 
EcoRV GAT'ATC

Cuts at:      0     177     279 
   Size:        177     102
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     108     140     279 
   Size:        108      32     139

  Fragments arranged by size: 

                 139     108      32
 
HincII GTy'rAC

Cuts at:      0       5     198     279 
   Size:          5     193      81

  Fragments arranged by size: 

                 193      81       5
 
HpaI GTT'AAC

Cuts at:      0     198     279 
   Size:        198      81
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     152     279 
   Size:        152     127
 
MslI CAynn'nnrTG

Cuts at:      0     220     279 
   Size:        220      59
 
PfoI T'CCnGG_A

Cuts at:      0     203     279 
   Size:        203      76
 
ScaI AGT'ACT

Cuts at:      0      55     279 
   Size:         55     224
 
SfcI C'TryA_G

Cuts at:      0      87     279 
   Size:         87     192
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0      22     133     279 
   Size:         22     111     146

  Fragments arranged by size: 

                 146     111      22
 
TatI w'GTAC_w

Cuts at:      0      53     279 
   Size:         53     226
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0      40      72     132     164     279 
   Size:         40      32      60      32     115

  Fragments arranged by size: 

                 115      60      40      32      32
 
ZraI GAC'GTC

Cuts at:      0      42     156     279 
   Size:         42     114     123

  Fragments arranged by size: 

                 123     114      42
 

 Enzymes that do cut:

    AatII     AccI     AcuI     AhdI     ApoI     BpmI     BsaI    BsaHI
    BsaXI     BsgI  Eco57MI    EcoNI    EcoRV    Hin4I   HincII     HpaI
     MmeI     MslI     PfoI     ScaI     SfcI    TaqII     TatI     TstI
     ZraI

 Enzymes that do not cut: 

     AarI   Acc65I     AclI     AfeI    AflII   AflIII     AgeI     AleI
     AlfI     AloI    AlwNI     ApaI    ApaLI     AscI     AseI    AsiSI
     AvaI    AvrII     BaeI    BamHI     BanI    BanII     BbeI     BbsI
    BbvCI     BcgI    BciVI     BclI    BfrBI     BglI    BglII     BlpI
 Bme1580I    BmgBI     BmrI     BmtI     BplI   Bpu10I    BpuEI    BsaAI
    BsaBI    BsaWI    BseRI    BseYI    BsiEI  BsiHKAI    BsiWI     BsmI
    BsmBI Bsp1286I    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrFI
    BsrGI   BssHII    BssSI   BstAPI    BstBI   BstEII    BstXI    BstYI
  BstZ17I   Bsu36I     BtgI    BtgZI     BtsI     ClaI    CspCI     DraI
   DraIII     DrdI     EaeI     EagI     EarI     EciI  EcoICRI EcoO109I
    EcoRI     FalI     FseI     FspI    FspAI    HaeII  HindIII     KasI
     KpnI     MfeI     MluI     MscI   MspA1I     NaeI     NarI     NcoI
     NdeI   NgoMIV     NheI     NotI     NruI     NsiI     NspI     PacI
     PasI     PciI    PflMI     PmeI     PmlI     PpiI    PpuMI    PshAI
     PsiI   PspOMI    PspXI     PsrI     PstI     PvuI    PvuII    RsrII
     SacI    SacII     SalI    SanDI     SapI     SbfI    SexAI     SfiI
     SfoI     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI
     SrfI     SspI     StuI     StyI     SwaI  Tth111I     XbaI     XcmI
     XhoI     XmaI     XmnI