Restriction map for
pFN21AE1391
(Linear) (Six-base) MAPSORT of: KIEE1391.seq Check: 244 from: 1 to: 2802
KIEE1391.orf
With 182 enzymes: SgfI *
July 15, 2010 18:37 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 2605 2802
Size: 2605 197
AccI GT'mk_AC
Cuts at: 0 1691 2802
Size: 1691 1111
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2034 2802
Size: 2034 768
AflIII A'CryG_T
Cuts at: 0 1218 2802
Size: 1218 1584
AhdI GACnn_n'nnGTC
Cuts at: 0 13 1613 2802
Size: 13 1600 1189
Fragments arranged by size:
1600 1189 13
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1556 2802
Size: 1556 1246
AlwNI CAG_nnn'CTG
Cuts at: 0 1609 2344 2802
Size: 1609 735 458
Fragments arranged by size:
1609 735 458
ApaLI G'TGCA_C
Cuts at: 0 1257 2802
Size: 1257 1545
ApoI r'AATT_y
Cuts at: 0 427 2006 2244 2802
Size: 427 1579 238 558
Fragments arranged by size:
1579 558 427 238
AvaI C'yCGr_G
Cuts at: 0 1757 2802
Size: 1757 1045
BamHI G'GATC_C
Cuts at: 0 944 2802
Size: 944 1858
BanI G'GyrC_C
Cuts at: 0 1636 2802
Size: 1636 1166
BanII G_rGCy'C
Cuts at: 0 611 832 1384 2802
Size: 611 221 552 1418
Fragments arranged by size:
1418 611 552 221
BbsI GAAGACnn'nnnn_
Cuts at: 0 1405 1706 1832 2378 2802
Size: 1405 301 126 546 424
Fragments arranged by size:
1405 546 424 301 126
BbvCI CC'TCA_GC
Cuts at: 0 1934 2802
Size: 1934 868
BciVI GTATCCnnnnn_n'
Cuts at: 0 2032 2802
Size: 2032 770
BclI T'GATC_A
Cuts at: 0 44 72 2802
Size: 44 28 2730
Fragments arranged by size:
2730 44 28
BfrBI ATG'CAT
Cuts at: 0 82 380 2351 2802
Size: 82 298 1971 451
Fragments arranged by size:
1971 451 298 82
BglI GCCn_nnn'nGGC
Cuts at: 0 2021 2201 2516 2802
Size: 2021 180 315 286
Fragments arranged by size:
2021 315 286 180
BglII A'GATC_T
Cuts at: 0 310 990 2394 2802
Size: 310 680 1404 408
Fragments arranged by size:
1404 680 408 310
Bme1580I G_kGCm'C
Cuts at: 0 1261 2802
Size: 1261 1541
BmrI ACTGGGnnnn_n'
Cuts at: 0 320 2616 2802
Size: 320 2296 186
Fragments arranged by size:
2296 320 186
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 662 1411 1652 1740 1931 2094 2348 2802
Size: 662 749 241 88 191 163 254 454
Fragments arranged by size:
749 662 454 254 241 191 163 88
Bpu10I CC'TnA_GC
Cuts at: 0 1934 2802
Size: 1934 868
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 798 2802
Size: 798 2004
BsaWI w'CCGG_w
Cuts at: 0 1964 2802
Size: 1964 838
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1371 1401 1713 1743 1985 2015 2401 2431
Size: 1371 30 312 30 242 30 386 30
Cuts at: 2431 2802
Size: 371
Fragments arranged by size:
1371 386 371 312 242 30 30 30
30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 629 1408 1802 2802
Size: 629 779 394 1000
Fragments arranged by size:
1000 779 629 394
BseYI C'CCAG_C
Cuts at: 0 2215 2322 2802
Size: 2215 107 480
Fragments arranged by size:
2215 480 107
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 979 1517 1815 2146 2802
Size: 979 538 298 331 656
Fragments arranged by size:
979 656 538 331 298
BsiEI CG_ry'CG
Cuts at: 0 2520 2802
Size: 2520 282
BsiHKAI G_wGCw'C
Cuts at: 0 832 1261 1384 1572 2802
Size: 832 429 123 188 1230
Fragments arranged by size:
1230 832 429 188 123
BsmI GAATG_Cn'
Cuts at: 0 42 380 475 1768 2098 2353 2802
Size: 42 338 95 1293 330 255 449
Fragments arranged by size:
1293 449 338 330 255 95 42
Bsp1286I G_dGCh'C
Cuts at: 0 611 832 1261 1384 1572 2802
Size: 611 221 429 123 188 1230
Fragments arranged by size:
1230 611 429 221 188 123
BspEI T'CCGG_A
Cuts at: 0 1964 2802
Size: 1964 838
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 2605 2802
Size: 2605 197
BsrDI GCAATG_nn'
Cuts at: 0 452 480 588 2802
Size: 452 28 108 2214
Fragments arranged by size:
2214 452 108 28
BsrGI T'GTAC_A
Cuts at: 0 567 1058 2802
Size: 567 491 1744
Fragments arranged by size:
1744 567 491
BstYI r'GATC_y
Cuts at: 0 310 944 990 2341 2394 2802
Size: 310 634 46 1351 53 408
Fragments arranged by size:
1351 634 408 310 53 46
Bsu36I CC'TnA_GG
Cuts at: 0 1519 2802
Size: 1519 1283
BtgI C'CryG_G
Cuts at: 0 265 2802
Size: 265 2537
BtsI GCAGTG_nn'
Cuts at: 0 1152 1505 2802
Size: 1152 353 1297
Fragments arranged by size:
1297 1152 353
DraI TTT'AAA
Cuts at: 0 2471 2802
Size: 2471 331
EaeI y'GGCC_r
Cuts at: 0 2 444 698 1587 2517 2802
Size: 2 442 254 889 930 285
Fragments arranged by size:
930 889 442 285 254 2
EagI C'GGCC_G
Cuts at: 0 2517 2802
Size: 2517 285
EarI CTCTTCn'nnn_
Cuts at: 0 401 2802
Size: 401 2401
EcoICRI GAG'CTC
Cuts at: 0 830 1382 2802
Size: 830 552 1420
Fragments arranged by size:
1420 830 552
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 662 1411 1652 1740 1931 2034 2094 2348
Size: 662 749 241 88 191 103 60 254
Cuts at: 2348 2802
Size: 454
Fragments arranged by size:
749 662 454 254 241 191 103 88
60
EcoO109I rG'GnC_Cy
Cuts at: 0 2069 2802
Size: 2069 733
EcoRI G'AATT_C
Cuts at: 0 2244 2802
Size: 2244 558
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 31 221 253 605 637 666 698 1371
Size: 31 190 32 352 32 29 32 673
Cuts at: 1371 1403 1583 1599 1615 1631 1648 1663 1680
Size: 32 180 16 16 16 17 15 17
Cuts at: 1680 1695 2401 2433 2802
Size: 15 706 32 369
Fragments arranged by size:
706 673 369 352 190 180 32 32
32 32 32 31 29 17 17 16
16 16 15 15
HindIII A'AGCT_T
Cuts at: 0 1526 2802
Size: 1526 1276
MluI A'CGCG_T
Cuts at: 0 1218 2802
Size: 1218 1584
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1971 2016 2444 2585 2802
Size: 1971 45 428 141 217
Fragments arranged by size:
1971 428 217 141 45
MscI TGG'CCA
Cuts at: 0 4 2802
Size: 4 2798
MslI CAynn'nnrTG
Cuts at: 0 2356 2802
Size: 2356 446
MspA1I CmG'CkG
Cuts at: 0 101 449 2322 2802
Size: 101 348 1873 480
Fragments arranged by size:
1873 480 348 101
NcoI C'CATG_G
Cuts at: 0 265 2802
Size: 265 2537
NsiI A_TGCA'T
Cuts at: 0 84 382 2353 2802
Size: 84 298 1971 449
Fragments arranged by size:
1971 449 298 84
NspI r_CATG'y
Cuts at: 0 2237 2802
Size: 2237 565
PasI CC'CwG_GG
Cuts at: 0 1110 2802
Size: 1110 1692
PflMI CCAn_nnn'nTGG
Cuts at: 0 1954 2802
Size: 1954 848
PfoI T'CCnGG_A
Cuts at: 0 1717 2248 2784 2802
Size: 1717 531 536 18
Fragments arranged by size:
1717 536 531 18
PpuMI rG'GwC_Cy
Cuts at: 0 2069 2802
Size: 2069 733
PshAI GACnn'nnGTC
Cuts at: 0 267 2802
Size: 267 2535
PsiI TTA'TAA
Cuts at: 0 1676 1742 2802
Size: 1676 66 1060
Fragments arranged by size:
1676 1060 66
PstI C_TGCA'G
Cuts at: 0 738 2802
Size: 738 2064
PvuII CAG'CTG
Cuts at: 0 101 2322 2802
Size: 101 2221 480
Fragments arranged by size:
2221 480 101
SacI G_AGCT'C
Cuts at: 0 832 1384 2802
Size: 832 552 1418
Fragments arranged by size:
1418 832 552
ScaI AGT'ACT
Cuts at: 0 163 2047 2802
Size: 163 1884 755
Fragments arranged by size:
1884 755 163
SfcI C'TryA_G
Cuts at: 0 734 2376 2802
Size: 734 1642 426
Fragments arranged by size:
1642 734 426
SmlI C'TyrA_G
Cuts at: 0 813 2802
Size: 813 1989
SspI AAT'ATT
Cuts at: 0 1070 2802
Size: 1070 1732
StyI C'CwwG_G
Cuts at: 0 265 2802
Size: 265 2537
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 2274 2802
Size: 2274 528
TatI w'GTAC_w
Cuts at: 0 20 161 567 1058 2045 2802
Size: 20 141 406 491 987 757
Fragments arranged by size:
987 757 491 406 141 20
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1240 1272 1713 1745 1785 1817 2802
Size: 1240 32 441 32 40 32 985
Fragments arranged by size:
1240 985 441 40 32 32 32
XbaI T'CTAG_A
Cuts at: 0 1038 2802
Size: 1038 1764
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1473 2069 2802
Size: 1473 596 733
Fragments arranged by size:
1473 733 596
Enzymes that do cut:
AarI AccI AcuI AflIII AhdI AlfI AlwNI ApaLI
ApoI AvaI BamHI BanI BanII BbsI BbvCI BciVI
BclI BfrBI BglI BglII Bme1580I BmrI BpmI Bpu10I
BpuEI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspMI BsrDI BsrGI BstYI Bsu36I
BtgI BtsI DraI EaeI EagI EarI EcoICRI Eco57MI
EcoO109I EcoRI Hin4I HindIII MluI MmeI MscI MslI
MspA1I NcoI NsiI NspI PasI PflMI PfoI PpuMI
PshAI PsiI PstI PvuII SacI ScaI SfcI SmlI
SspI StyI TaqII TatI TstI XbaI XcmI
Enzymes that do not cut:
AatII Acc65I AclI AfeI AflII AgeI AleI AloI
ApaI AscI AseI AsiSI AvrII BaeI BbeI BcgI
BlpI BmgBI BmtI BplI BsaI BsaAI BsaBI BsaHI
BsiWI BsmBI BspHI BsrBI BsrFI BssHII BssSI BstAPI
BstBI BstEII BstXI BstZ17I BtgZI ClaI CspCI DraIII
DrdI EciI EcoNI EcoRV FalI FseI FspI FspAI
HaeII HincII HpaI KasI KpnI MfeI NaeI NarI
NdeI NgoMIV NheI NotI NruI PacI PciI PmeI
PmlI PpiI PspOMI PspXI PsrI PvuI RsrII SacII
SalI SanDI SapI SbfI SexAI SfiI SfoI SgfI
SgrAI SmaI SnaBI SpeI SphI SrfI StuI SwaI
Tth111I XhoI XmaI XmnI ZraI