Restriction map for
pFN21AE1390
(Linear) (Six-base) MAPSORT of: KIEE1390.seq Check: 2420 from: 1 to: 1131
KIEE1390.orf
With 182 enzymes: SgfI *
July 15, 2010 18:37 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 226 1131
Size: 226 905
AfeI AGC'GCT
Cuts at: 0 766 1131
Size: 766 365
AvaI C'yCGr_G
Cuts at: 0 298 1131
Size: 298 833
BanI G'GyrC_C
Cuts at: 0 122 263 910 1131
Size: 122 141 647 221
Fragments arranged by size:
647 221 141 122
BanII G_rGCy'C
Cuts at: 0 287 1036 1131
Size: 287 749 95
Fragments arranged by size:
749 287 95
BbsI GAAGACnn'nnnn_
Cuts at: 0 87 1131
Size: 87 1044
BciVI GTATCCnnnnn_n'
Cuts at: 0 221 1131
Size: 221 910
BglI GCCn_nnn'nGGC
Cuts at: 0 415 1131
Size: 415 716
BglII A'GATC_T
Cuts at: 0 257 1131
Size: 257 874
BmrI ACTGGGnnnn_n'
Cuts at: 0 249 1131
Size: 249 882
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 733 926 1131
Size: 733 193 205
Fragments arranged by size:
733 205 193
Bpu10I CC'TnA_GC
Cuts at: 0 1004 1131
Size: 1004 127
BsaI GGTCTCn'nnnn_
Cuts at: 0 10 373 1131
Size: 10 363 758
Fragments arranged by size:
758 363 10
BsaAI yAC'GTr
Cuts at: 0 165 405 1131
Size: 165 240 726
Fragments arranged by size:
726 240 165
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 122 152 1131
Size: 122 30 979
Fragments arranged by size:
979 122 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 28 316 696 1131
Size: 28 288 380 435
Fragments arranged by size:
435 380 288 28
BsiHKAI G_wGCw'C
Cuts at: 0 287 308 1131
Size: 287 21 823
Fragments arranged by size:
823 287 21
Bsp1286I G_dGCh'C
Cuts at: 0 287 308 1036 1131
Size: 287 21 728 95
Fragments arranged by size:
728 287 95 21
BspHI T'CATG_A
Cuts at: 0 572 851 1131
Size: 572 279 280
Fragments arranged by size:
572 280 279
BsrDI GCAATG_nn'
Cuts at: 0 765 1131
Size: 765 366
BsrFI r'CCGG_y
Cuts at: 0 61 1131
Size: 61 1070
BstXI CCAn_nnnn'nTGG
Cuts at: 0 27 405 1131
Size: 27 378 726
Fragments arranged by size:
726 378 27
BstYI r'GATC_y
Cuts at: 0 257 540 578 1131
Size: 257 283 38 553
Fragments arranged by size:
553 283 257 38
BtgI C'CryG_G
Cuts at: 0 118 1131
Size: 118 1013
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 89 1131
Size: 89 1042
BtsI GCAGTG_nn'
Cuts at: 0 425 611 956 1131
Size: 425 186 345 175
Fragments arranged by size:
425 345 186 175
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 438 473 1131
Size: 438 35 658
Fragments arranged by size:
658 438 35
EaeI y'GGCC_r
Cuts at: 0 407 441 686 738 1131
Size: 407 34 245 52 393
Fragments arranged by size:
407 393 245 52 34
EarI CTCTTCn'nnn_
Cuts at: 0 705 712 793 1131
Size: 705 7 81 338
Fragments arranged by size:
705 338 81 7
EcoICRI GAG'CTC
Cuts at: 0 285 1131
Size: 285 846
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 226 733 926 1131
Size: 226 507 193 205
Fragments arranged by size:
507 226 205 193
EcoO109I rG'GnC_Cy
Cuts at: 0 327 555 879 1131
Size: 327 228 324 252
Fragments arranged by size:
327 324 252 228
EcoRV GAT'ATC
Cuts at: 0 870 1131
Size: 870 261
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 81 1131
Size: 81 1050
HaeII r_GCGC'y
Cuts at: 0 768 1131
Size: 768 363
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 28 60 981 1013 1131
Size: 28 32 921 32 118
Fragments arranged by size:
921 118 32 32 28
MscI TGG'CCA
Cuts at: 0 409 688 740 1131
Size: 409 279 52 391
Fragments arranged by size:
409 391 279 52
MslI CAynn'nnrTG
Cuts at: 0 403 808 916 1131
Size: 403 405 108 215
Fragments arranged by size:
405 403 215 108
MspA1I CmG'CkG
Cuts at: 0 120 333 694 773 1131
Size: 120 213 361 79 358
Fragments arranged by size:
361 358 213 120 79
NaeI GCC'GGC
Cuts at: 0 63 1131
Size: 63 1068
NgoMIV G'CCGG_C
Cuts at: 0 61 1131
Size: 61 1070
PpuMI rG'GwC_Cy
Cuts at: 0 555 879 1131
Size: 555 324 252
Fragments arranged by size:
555 324 252
PvuII CAG'CTG
Cuts at: 0 694 1131
Size: 694 437
SacI G_AGCT'C
Cuts at: 0 287 1131
Size: 287 844
SacII CC_GC'GG
Cuts at: 0 121 1131
Size: 121 1010
SapI GCTCTTCn'nnn_
Cuts at: 0 712 1131
Size: 712 419
SnaBI TAC'GTA
Cuts at: 0 165 1131
Size: 165 966
StyI C'CwwG_G
Cuts at: 0 341 1131
Size: 341 790
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 122 154 1131
Size: 122 32 977
Fragments arranged by size:
977 122 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 534 1131
Size: 534 597
Enzymes that do cut:
AcuI AfeI AvaI BanI BanII BbsI BciVI BglI
BglII BmrI BpmI Bpu10I BsaI BsaAI BsaXI BseRI
BsiHKAI Bsp1286I BspHI BsrDI BsrFI BstXI BstYI BtgI
BtgZI BtsI CspCI EaeI EarI EcoICRI Eco57MI EcoO109I
EcoRV FalI HaeII Hin4I MscI MslI MspA1I NaeI
NgoMIV PpuMI PvuII SacI SacII SapI SnaBI StyI
TstI XcmI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI
AscI AseI AsiSI AvrII BaeI BamHI BbeI BbvCI
BcgI BclI BfrBI BlpI Bme1580I BmgBI BmtI BplI
BpuEI BsaBI BsaHI BsaWI BseYI BsgI BsiEI BsiWI
BsmI BsmBI BspEI BspMI BsrBI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstZ17I Bsu36I ClaI DraI DraIII
DrdI EagI EciI EcoNI EcoRI FseI FspI FspAI
HincII HindIII HpaI KasI KpnI MfeI MluI MmeI
NarI NcoI NdeI NheI NotI NruI NsiI NspI
PacI PasI PciI PflMI PfoI PmeI PmlI PpiI
PshAI PsiI PspOMI PspXI PsrI PstI PvuI RsrII
SalI SanDI SbfI ScaI SexAI SfcI SfiI SfoI
SgfI SgrAI SmaI SmlI SpeI SphI SrfI SspI
StuI SwaI TaqII TatI Tth111I XbaI XhoI XmaI
XmnI ZraI