Restriction map for
pFN21AE1389
(Linear) (Six-base) MAPSORT of: KIEE1389.seq Check: 1059 from: 1 to: 1149
KIEE1389.orf
With 182 enzymes: SgfI *
July 15, 2010 18:37 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 773 1149
Size: 773 376
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 650 1149
Size: 650 499
AleI CACnn'nnGTG
Cuts at: 0 640 1149
Size: 640 509
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 320 1149
Size: 320 829
ApaI G_GGCC'C
Cuts at: 0 152 1149
Size: 152 997
ApoI r'AATT_y
Cuts at: 0 271 467 502 1149
Size: 271 196 35 647
Fragments arranged by size:
647 271 196 35
AvaI C'yCGr_G
Cuts at: 0 956 1149
Size: 956 193
BanII G_rGCy'C
Cuts at: 0 152 406 1149
Size: 152 254 743
Fragments arranged by size:
743 254 152
BbsI GAAGACnn'nnnn_
Cuts at: 0 587 1149
Size: 587 562
BbvCI CC'TCA_GC
Cuts at: 0 288 696 1149
Size: 288 408 453
Fragments arranged by size:
453 408 288
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 837 871 1149
Size: 837 34 278
Fragments arranged by size:
837 278 34
Bme1580I G_kGCm'C
Cuts at: 0 152 1149
Size: 152 997
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 751 1149
Size: 751 398
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 209 457 608 1149
Size: 209 248 151 541
Fragments arranged by size:
541 248 209 151
Bpu10I CC'TnA_GC
Cuts at: 0 288 696 1149
Size: 288 408 453
Fragments arranged by size:
453 408 288
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 550 737 1149
Size: 550 187 412
Fragments arranged by size:
550 412 187
BsaI GGTCTCn'nnnn_
Cuts at: 0 953 1149
Size: 953 196
BsaAI yAC'GTr
Cuts at: 0 789 1149
Size: 789 360
BsaWI w'CCGG_w
Cuts at: 0 65 1149
Size: 65 1084
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 433 463 581 611 1149
Size: 433 30 118 30 538
Fragments arranged by size:
538 433 118 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 454 595 684 740 1149
Size: 454 141 89 56 409
Fragments arranged by size:
454 409 141 89 56
BseYI C'CCAG_C
Cuts at: 0 475 711 1149
Size: 475 236 438
Fragments arranged by size:
475 438 236
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 114 157 802 1149
Size: 114 43 645 347
Fragments arranged by size:
645 347 114 43
BsiHKAI G_wGCw'C
Cuts at: 0 406 1149
Size: 406 743
Bsp1286I G_dGCh'C
Cuts at: 0 152 406 1149
Size: 152 254 743
Fragments arranged by size:
743 254 152
BspEI T'CCGG_A
Cuts at: 0 65 1149
Size: 65 1084
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 773 947 1149
Size: 773 174 202
Fragments arranged by size:
773 202 174
BsrGI T'GTAC_A
Cuts at: 0 927 1149
Size: 927 222
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1132 1149
Size: 1132 17
DraI TTT'AAA
Cuts at: 0 168 1149
Size: 168 981
EaeI y'GGCC_r
Cuts at: 0 1073 1149
Size: 1073 76
EarI CTCTTCn'nnn_
Cuts at: 0 707 807 1149
Size: 707 100 342
Fragments arranged by size:
707 342 100
EcoICRI GAG'CTC
Cuts at: 0 404 1149
Size: 404 745
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 209 457 608 650 1149
Size: 209 248 151 42 499
Fragments arranged by size:
499 248 209 151 42
EcoNI CCTnn'n_nnAGG
Cuts at: 0 565 1149
Size: 565 584
EcoRI G'AATT_C
Cuts at: 0 271 1149
Size: 271 878
EcoRV GAT'ATC
Cuts at: 0 210 1149
Size: 210 939
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 675 1149
Size: 675 474
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 106 138 1149
Size: 106 32 1011
Fragments arranged by size:
1011 106 32
MscI TGG'CCA
Cuts at: 0 1075 1149
Size: 1075 74
MslI CAynn'nnrTG
Cuts at: 0 201 640 1149
Size: 201 439 509
Fragments arranged by size:
509 439 201
NdeI CA'TA_TG
Cuts at: 0 183 1149
Size: 183 966
NspI r_CATG'y
Cuts at: 0 682 1149
Size: 682 467
PflMI CCAn_nnn'nTGG
Cuts at: 0 187 451 1149
Size: 187 264 698
Fragments arranged by size:
698 264 187
PfoI T'CCnGG_A
Cuts at: 0 310 395 720 1118 1149
Size: 310 85 325 398 31
Fragments arranged by size:
398 325 310 85 31
PspOMI G'GGCC_C
Cuts at: 0 148 1149
Size: 148 1001
SacI G_AGCT'C
Cuts at: 0 406 1149
Size: 406 743
SapI GCTCTTCn'nnn_
Cuts at: 0 707 1149
Size: 707 442
SmlI C'TyrA_G
Cuts at: 0 565 752 956 1149
Size: 565 187 204 193
Fragments arranged by size:
565 204 193 187
SphI G_CATG'C
Cuts at: 0 682 1149
Size: 682 467
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 176 652 1149
Size: 176 476 497
Fragments arranged by size:
497 476 176
TatI w'GTAC_w
Cuts at: 0 656 927 1149
Size: 656 271 222
Fragments arranged by size:
656 271 222
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 581 613 1149
Size: 581 32 536
Fragments arranged by size:
581 536 32
XhoI C'TCGA_G
Cuts at: 0 956 1149
Size: 956 193
Enzymes that do cut:
AarI AcuI AleI AlfI ApaI ApoI AvaI BanII
BbsI BbvCI BcgI Bme1580I BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaWI BsaXI BseRI BseYI BsgI BsiHKAI
Bsp1286I BspEI BspMI BsrGI BstAPI DraI EaeI EarI
EcoICRI Eco57MI EcoNI EcoRI EcoRV FalI Hin4I MscI
MslI NdeI NspI PflMI PfoI PspOMI SacI SapI
SmlI SphI TaqII TatI TstI XhoI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflII AflIII AgeI
AhdI AloI AlwNI ApaLI AscI AseI AsiSI AvrII
BaeI BamHI BanI BbeI BciVI BclI BfrBI BglI
BglII BlpI BmgBI BmrI BmtI BsaBI BsaHI BsiEI
BsiWI BsmI BsmBI BspHI BsrBI BsrDI BsrFI BssHII
BssSI BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtgI
BtgZI BtsI ClaI CspCI DraIII DrdI EagI EciI
EcoO109I FseI FspI FspAI HaeII HincII HindIII HpaI
KasI KpnI MfeI MluI MmeI MspA1I NaeI NarI
NcoI NgoMIV NheI NotI NruI NsiI PacI PasI
PciI PmeI PmlI PpiI PpuMI PshAI PsiI PspXI
PsrI PstI PvuI PvuII RsrII SacII SalI SanDI
SbfI ScaI SexAI SfcI SfiI SfoI SgfI SgrAI
SmaI SnaBI SpeI SrfI SspI StuI StyI SwaI
Tth111I XbaI XcmI XmaI XmnI ZraI