Restriction map for
pFN21AE1387
(Linear) (Six-base) MAPSORT of: KIEE1387.seq Check: 8009 from: 1 to: 2946
KIEE1387.orf
With 182 enzymes: SgfI *
July 15, 2010 18:37 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 2346 2946
Size: 2346 600
AccI GT'mk_AC
Cuts at: 0 1716 1821 2946
Size: 1716 105 1125
Fragments arranged by size:
1716 1125 105
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 178 432 2234 2549 2946
Size: 178 254 1802 315 397
Fragments arranged by size:
1802 397 315 254 178
AflII C'TTAA_G
Cuts at: 0 1650 2277 2946
Size: 1650 627 669
Fragments arranged by size:
1650 669 627
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1351 2946
Size: 1351 1595
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 2226 2258 2946
Size: 2226 32 688
Fragments arranged by size:
2226 688 32
AlwNI CAG_nnn'CTG
Cuts at: 0 532 2317 2946
Size: 532 1785 629
Fragments arranged by size:
1785 629 532
ApaLI G'TGCA_C
Cuts at: 0 1176 1407 2946
Size: 1176 231 1539
Fragments arranged by size:
1539 1176 231
ApoI r'AATT_y
Cuts at: 0 62 538 694 750 2073 2157 2747 2905
Size: 62 476 156 56 1323 84 590 158
Cuts at: 2905 2946
Size: 41
Fragments arranged by size:
1323 590 476 158 156 84 62 56
41
AvaI C'yCGr_G
Cuts at: 0 1784 2354 2946
Size: 1784 570 592
Fragments arranged by size:
1784 592 570
AvrII C'CTAG_G
Cuts at: 0 1827 2946
Size: 1827 1119
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 498 531 557 590 2946
Size: 498 33 26 33 2356
Fragments arranged by size:
2356 498 33 33 26
BamHI G'GATC_C
Cuts at: 0 1269 1444 2946
Size: 1269 175 1502
Fragments arranged by size:
1502 1269 175
BanI G'GyrC_C
Cuts at: 0 1778 2700 2946
Size: 1778 922 246
Fragments arranged by size:
1778 922 246
BanII G_rGCy'C
Cuts at: 0 733 1102 1259 1840 1900 2946
Size: 733 369 157 581 60 1046
Fragments arranged by size:
1046 733 581 369 157 60
BbsI GAAGACnn'nnnn_
Cuts at: 0 148 1586 2946
Size: 148 1438 1360
Fragments arranged by size:
1438 1360 148
BciVI GTATCCnnnnn_n'
Cuts at: 0 2937 2946
Size: 2937 9
BclI T'GATC_A
Cuts at: 0 2667 2946
Size: 2667 279
BglI GCCn_nnn'nGGC
Cuts at: 0 1164 2946
Size: 1164 1782
BglII A'GATC_T
Cuts at: 0 1558 2946
Size: 1558 1388
Bme1580I G_kGCm'C
Cuts at: 0 1180 1411 1783 2946
Size: 1180 231 372 1163
Fragments arranged by size:
1180 1163 372 231
BmgBI CAC'GTC
Cuts at: 0 2935 2946
Size: 2935 11
BmrI ACTGGGnnnn_n'
Cuts at: 0 911 1900 2095 2946
Size: 911 989 195 851
Fragments arranged by size:
989 911 851 195
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 564 1281 1474 2009 2505 2906 2946
Size: 564 717 193 535 496 401 40
Fragments arranged by size:
717 564 535 496 401 193 40
Bpu10I CC'TnA_GC
Cuts at: 0 107 1955 2946
Size: 107 1848 991
Fragments arranged by size:
1848 991 107
BsaI GGTCTCn'nnnn_
Cuts at: 0 2090 2946
Size: 2090 856
BsaBI GATnn'nnATC
Cuts at: 0 242 1358 2405 2946
Size: 242 1116 1047 541
Fragments arranged by size:
1116 1047 541 242
BsaHI Gr'CG_yC
Cuts at: 0 1929 2392 2946
Size: 1929 463 554
Fragments arranged by size:
1929 554 463
BsaWI w'CCGG_w
Cuts at: 0 854 2946
Size: 854 2092
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 379 409 1943 1973 2946
Size: 379 30 1534 30 973
Fragments arranged by size:
1534 973 379 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 53 1017 1381 1514 1634 1652 2006 2009
Size: 53 964 364 133 120 18 354 3
Cuts at: 2009 2012 2029 2909 2946
Size: 3 17 880 37
Fragments arranged by size:
964 880 364 354 133 120 53 37
18 17 3 3
BseYI C'CCAG_C
Cuts at: 0 1632 2946
Size: 1632 1314
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1148 2723 2946
Size: 1148 1575 223
Fragments arranged by size:
1575 1148 223
BsiEI CG_ry'CG
Cuts at: 0 2728 2946
Size: 2728 218
BsiHKAI G_wGCw'C
Cuts at: 0 1180 1411 2946
Size: 1180 231 1535
Fragments arranged by size:
1535 1180 231
Bsp1286I G_dGCh'C
Cuts at: 0 733 1102 1180 1259 1411 1783 1840 1900
Size: 733 369 78 79 152 372 57 60
Cuts at: 1900 2946
Size: 1046
Fragments arranged by size:
1046 733 372 369 152 79 78 60
57
BspHI T'CATG_A
Cuts at: 0 603 1047 2946
Size: 603 444 1899
Fragments arranged by size:
1899 603 444
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 2346 2946
Size: 2346 600
BsrDI GCAATG_nn'
Cuts at: 0 644 678 2946
Size: 644 34 2268
Fragments arranged by size:
2268 644 34
BsrFI r'CCGG_y
Cuts at: 0 2528 2946
Size: 2528 418
BstAPI GCAn_nnn'nTGC
Cuts at: 0 532 1184 2806 2946
Size: 532 652 1622 140
Fragments arranged by size:
1622 652 532 140
BstEII G'GTnAC_C
Cuts at: 0 1972 2621 2946
Size: 1972 649 325
Fragments arranged by size:
1972 649 325
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1266 2946
Size: 1266 1680
BstYI r'GATC_y
Cuts at: 0 1269 1444 1493 1533 1558 1656 2547 2568
Size: 1269 175 49 40 25 98 891 21
Cuts at: 2568 2946
Size: 378
Fragments arranged by size:
1269 891 378 175 98 49 40 25
21
Bsu36I CC'TnA_GG
Cuts at: 0 2538 2774 2946
Size: 2538 236 172
Fragments arranged by size:
2538 236 172
BtgI C'CryG_G
Cuts at: 0 134 577 2766 2946
Size: 134 443 2189 180
Fragments arranged by size:
2189 443 180 134
DraI TTT'AAA
Cuts at: 0 321 375 2291 2946
Size: 321 54 1916 655
Fragments arranged by size:
1916 655 321 54
DrdI GACnn_nn'nnGTC
Cuts at: 0 1144 2946
Size: 1144 1802
EaeI y'GGCC_r
Cuts at: 0 2530 2592 2946
Size: 2530 62 354
Fragments arranged by size:
2530 354 62
EarI CTCTTCn'nnn_
Cuts at: 0 2834 2842 2946
Size: 2834 8 104
Fragments arranged by size:
2834 104 8
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 178 432 564 1281 1474 2009 2234 2505
Size: 178 254 132 717 193 535 225 271
Cuts at: 2505 2549 2906 2946
Size: 44 357 40
Fragments arranged by size:
717 535 357 271 254 225 193 178
132 44 40
EcoNI CCTnn'n_nnAGG
Cuts at: 0 604 1831 1946 2423 2946
Size: 604 1227 115 477 523
Fragments arranged by size:
1227 604 523 477 115
EcoRI G'AATT_C
Cuts at: 0 538 2157 2946
Size: 538 1619 789
Fragments arranged by size:
1619 789 538
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1760 2871 2946
Size: 1760 1111 75
Fragments arranged by size:
1760 1111 75
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 410 442 915 947 996 1028 1279 1311
Size: 410 32 473 32 49 32 251 32
Cuts at: 1311 1368 1400 1485 1517 1808 1840 1943 1975
Size: 57 32 85 32 291 32 103 32
Cuts at: 1975 2245 2277 2299 2331 2946
Size: 270 32 22 32 615
Fragments arranged by size:
615 473 410 291 270 251 103 85
57 49 32 32 32 32 32 32
32 32 32 32 22
HincII GTy'rAC
Cuts at: 0 1822 2946
Size: 1822 1124
MfeI C'AATT_G
Cuts at: 0 656 2946
Size: 656 2290
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 78 1187 2374 2946
Size: 78 1109 1187 572
Fragments arranged by size:
1187 1109 572 78
MscI TGG'CCA
Cuts at: 0 2594 2946
Size: 2594 352
MslI CAynn'nnrTG
Cuts at: 0 1264 1333 2946
Size: 1264 69 1613
Fragments arranged by size:
1613 1264 69
NaeI GCC'GGC
Cuts at: 0 2530 2946
Size: 2530 416
NcoI C'CATG_G
Cuts at: 0 577 2766 2946
Size: 577 2189 180
Fragments arranged by size:
2189 577 180
NdeI CA'TA_TG
Cuts at: 0 795 2946
Size: 795 2151
NgoMIV G'CCGG_C
Cuts at: 0 2528 2946
Size: 2528 418
NspI r_CATG'y
Cuts at: 0 459 1332 2946
Size: 459 873 1614
Fragments arranged by size:
1614 873 459
PflMI CCAn_nnn'nTGG
Cuts at: 0 1731 1849 2371 2946
Size: 1731 118 522 575
Fragments arranged by size:
1731 575 522 118
PsiI TTA'TAA
Cuts at: 0 1010 2946
Size: 1010 1936
SalI G'TCGA_C
Cuts at: 0 1820 2946
Size: 1820 1126
ScaI AGT'ACT
Cuts at: 0 316 2946
Size: 316 2630
SexAI A'CCwGG_T
Cuts at: 0 2625 2946
Size: 2625 321
SfcI C'TryA_G
Cuts at: 0 817 2946
Size: 817 2129
SmlI C'TyrA_G
Cuts at: 0 1650 2277 2946
Size: 1650 627 669
Fragments arranged by size:
1650 669 627
SphI G_CATG'C
Cuts at: 0 1332 2946
Size: 1332 1614
StuI AGG'CCT
Cuts at: 0 2346 2946
Size: 2346 600
StyI C'CwwG_G
Cuts at: 0 577 724 1827 1977 2766 2946
Size: 577 147 1103 150 789 180
Fragments arranged by size:
1103 789 577 180 150 147
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 2189 2946
Size: 2189 757
TatI w'GTAC_w
Cuts at: 0 314 2056 2946
Size: 314 1742 890
Fragments arranged by size:
1742 890 314
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1478 1510 2134 2166 2946
Size: 1478 32 624 32 780
Fragments arranged by size:
1478 780 624 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 622 2946
Size: 622 2324
XmnI GAAnn'nnTTC
Cuts at: 0 586 2946
Size: 586 2360
Enzymes that do cut:
AarI AccI AcuI AflII AlfI AloI AlwNI ApaLI
ApoI AvaI AvrII BaeI BamHI BanI BanII BbsI
BciVI BclI BglI BglII Bme1580I BmgBI BmrI BpmI
Bpu10I BsaI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI Bsp1286I BspHI BspMI BsrDI BsrFI
BstAPI BstEII BstXI BstYI Bsu36I BtgI DraI DrdI
EaeI EarI Eco57MI EcoNI EcoRI FalI Hin4I HincII
MfeI MmeI MscI MslI NaeI NcoI NdeI NgoMIV
NspI PflMI PsiI SalI ScaI SexAI SfcI SmlI
SphI StuI StyI TaqII TatI TstI XcmI XmnI
Enzymes that do not cut:
AatII Acc65I AclI AfeI AflIII AgeI AhdI AleI
ApaI AscI AseI AsiSI BbeI BbvCI BcgI BfrBI
BlpI BmtI BplI BpuEI BsaAI BsiWI BsmI BsmBI
BspEI BsrBI BsrGI BssHII BssSI BstBI BstZ17I BtgZI
BtsI ClaI CspCI DraIII EagI EciI EcoICRI EcoO109I
EcoRV FseI FspI FspAI HaeII HindIII HpaI KasI
KpnI MluI MspA1I NarI NheI NotI NruI NsiI
PacI PasI PciI PfoI PmeI PmlI PpiI PpuMI
PshAI PspOMI PspXI PsrI PstI PvuI PvuII RsrII
SacI SacII SanDI SapI SbfI SfiI SfoI SgfI
SgrAI SmaI SnaBI SpeI SrfI SspI SwaI Tth111I
XbaI XhoI XmaI ZraI