Restriction map for
pFN21AE1386
(Linear) (Six-base) MAPSORT of: KIEE1386.seq Check: 2196 from: 1 to: 615
KIEE1386.orf
With 182 enzymes: SgfI *
July 15, 2010 18:37 ..
AccI GT'mk_AC
Cuts at: 0 326 615
Size: 326 289
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 110 615
Size: 110 505
AhdI GACnn_n'nnGTC
Cuts at: 0 143 615
Size: 143 472
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 5 37 615
Size: 5 32 578
Fragments arranged by size:
578 32 5
ApaI G_GGCC'C
Cuts at: 0 96 615
Size: 96 519
BanI G'GyrC_C
Cuts at: 0 40 615
Size: 40 575
BanII G_rGCy'C
Cuts at: 0 96 104 615
Size: 96 8 511
Fragments arranged by size:
511 96 8
BbsI GAAGACnn'nnnn_
Cuts at: 0 333 585 615
Size: 333 252 30
Fragments arranged by size:
333 252 30
BbvCI CC'TCA_GC
Cuts at: 0 445 577 615
Size: 445 132 38
Fragments arranged by size:
445 132 38
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 303 337 615
Size: 303 34 278
Fragments arranged by size:
303 278 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 476 615
Size: 476 139
BfrBI ATG'CAT
Cuts at: 0 162 615
Size: 162 453
Bme1580I G_kGCm'C
Cuts at: 0 96 615
Size: 96 519
BmrI ACTGGGnnnn_n'
Cuts at: 0 414 615
Size: 414 201
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 247 255 615
Size: 247 8 360
Fragments arranged by size:
360 247 8
Bpu10I CC'TnA_GC
Cuts at: 0 445 577 615
Size: 445 132 38
Fragments arranged by size:
445 132 38
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 300 615
Size: 300 315
BsaI GGTCTCn'nnnn_
Cuts at: 0 200 527 615
Size: 200 327 88
Fragments arranged by size:
327 200 88
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 41 71 615
Size: 41 30 544
Fragments arranged by size:
544 41 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 46 366 405 433 615
Size: 46 320 39 28 182
Fragments arranged by size:
320 182 46 39 28
BseYI C'CCAG_C
Cuts at: 0 211 615
Size: 211 404
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 475 615
Size: 475 140
BsmBI CGTCTCn'nnnn_
Cuts at: 0 22 615
Size: 22 593
Bsp1286I G_dGCh'C
Cuts at: 0 96 104 615
Size: 96 8 511
Fragments arranged by size:
511 96 8
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 195 615
Size: 195 420
BstXI CCAn_nnnn'nTGG
Cuts at: 0 556 615
Size: 556 59
BtgI C'CryG_G
Cuts at: 0 504 615
Size: 504 111
BtsI GCAGTG_nn'
Cuts at: 0 226 615
Size: 226 389
EarI CTCTTCn'nnn_
Cuts at: 0 25 415 615
Size: 25 390 200
Fragments arranged by size:
390 200 25
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 110 247 255 615
Size: 110 137 8 360
Fragments arranged by size:
360 137 110 8
EcoO109I rG'GnC_Cy
Cuts at: 0 92 615
Size: 92 523
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 129 161 259 291 450 482 526 558
Size: 129 32 98 32 159 32 44 32
Cuts at: 558 615
Size: 57
Fragments arranged by size:
159 129 98 57 44 32 32 32
32
MslI CAynn'nnrTG
Cuts at: 0 554 615
Size: 554 61
MspA1I CmG'CkG
Cuts at: 0 130 615
Size: 130 485
NsiI A_TGCA'T
Cuts at: 0 164 615
Size: 164 451
PasI CC'CwG_GG
Cuts at: 0 96 528 615
Size: 96 432 87
Fragments arranged by size:
432 96 87
PflMI CCAn_nnn'nTGG
Cuts at: 0 459 615
Size: 459 156
PfoI T'CCnGG_A
Cuts at: 0 263 615
Size: 263 352
PspOMI G'GGCC_C
Cuts at: 0 92 615
Size: 92 523
PstI C_TGCA'G
Cuts at: 0 479 615
Size: 479 136
PvuII CAG'CTG
Cuts at: 0 130 615
Size: 130 485
RsrII CG'GwC_CG
Cuts at: 0 249 615
Size: 249 366
SapI GCTCTTCn'nnn_
Cuts at: 0 415 615
Size: 415 200
ScaI AGT'ACT
Cuts at: 0 588 615
Size: 588 27
SfcI C'TryA_G
Cuts at: 0 475 615
Size: 475 140
SmlI C'TyrA_G
Cuts at: 0 279 615
Size: 279 336
StyI C'CwwG_G
Cuts at: 0 28 365 615
Size: 28 337 250
Fragments arranged by size:
337 250 28
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 541 562 615
Size: 541 21 53
Fragments arranged by size:
541 53 21
TatI w'GTAC_w
Cuts at: 0 586 615
Size: 586 29
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 103 615
Size: 103 512
Enzymes that do cut:
AccI AcuI AhdI AloI ApaI BanI BanII BbsI
BbvCI BcgI BciVI BfrBI Bme1580I BmrI BpmI Bpu10I
BpuEI BsaI BsaXI BseRI BseYI BsgI BsmBI Bsp1286I
BspMI BstXI BtgI BtsI EarI Eco57MI EcoO109I Hin4I
MslI MspA1I NsiI PasI PflMI PfoI PspOMI PstI
PvuII RsrII SapI ScaI SfcI SmlI StyI TaqII
TatI XcmI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AflIII AgeI
AleI AlfI AlwNI ApaLI ApoI AscI AseI AsiSI
AvaI AvrII BaeI BamHI BbeI BclI BglI BglII
BlpI BmgBI BmtI BplI BsaAI BsaBI BsaHI BsaWI
BsiEI BsiHKAI BsiWI BsmI BspEI BspHI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI
BstZ17I Bsu36I BtgZI ClaI CspCI DraI DraIII DrdI
EaeI EagI EciI EcoICRI EcoNI EcoRI EcoRV FalI
FseI FspI FspAI HaeII HincII HindIII HpaI KasI
KpnI MfeI MluI MmeI MscI NaeI NarI NcoI
NdeI NgoMIV NheI NotI NruI NspI PacI PciI
PmeI PmlI PpiI PpuMI PshAI PsiI PspXI PsrI
PvuI SacI SacII SalI SanDI SbfI SexAI SfiI
SfoI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI
SspI StuI SwaI TstI Tth111I XbaI XhoI XmaI
XmnI ZraI