(Linear) (Six-base) MAPSORT of: KIEE1385.seq  Check: 2007  from: 1  to: 375



KIEE1385.orf

 With 182 enzymes: SgfI * 

                        July 15, 2010 18:37  ..

AccI GT'mk_AC

Cuts at:      0     332     375 
   Size:        332      43
 
ApaI G_GGCC'C

Cuts at:      0     225     267     375 
   Size:        225      42     108

  Fragments arranged by size: 

                 225     108      42
 
BanI G'GyrC_C

Cuts at:      0      36     375 
   Size:         36     339
 
BanII G_rGCy'C

Cuts at:      0     225     267     375 
   Size:        225      42     108

  Fragments arranged by size: 

                 225     108      42
 
BclI T'GATC_A

Cuts at:      0      71     375 
   Size:         71     304
 
Bme1580I G_kGCm'C

Cuts at:      0      39     101     225     267     375 
   Size:         39      62     124      42     108

  Fragments arranged by size: 

                 124     108      62      42      39
 
BmgBI CAC'GTC

Cuts at:      0     213     375 
   Size:        213     162
 
BsaI GGTCTCn'nnnn_

Cuts at:      0      82     375 
   Size:         82     293
 
BsaBI GATnn'nnATC

Cuts at:      0      76     375 
   Size:         76     299
 
BseYI C'CCAG_C

Cuts at:      0      30      43     375 
   Size:         30      13     332

  Fragments arranged by size: 

                 332      30      13
 
BsmI GAATG_Cn'

Cuts at:      0     166     375 
   Size:        166     209
 
Bsp1286I G_dGCh'C

Cuts at:      0      39     101     225     267     375 
   Size:         39      62     124      42     108

  Fragments arranged by size: 

                 124     108      62      42      39
 
BspHI T'CATG_A

Cuts at:      0     284     375 
   Size:        284      91
 
DrdI GACnn_nn'nnGTC

Cuts at:      0     109     375 
   Size:        109     266
 
EarI CTCTTCn'nnn_

Cuts at:      0     139     375 
   Size:        139     236
 
EcoO109I rG'GnC_Cy

Cuts at:      0     221     264     375 
   Size:        221      43     111

  Fragments arranged by size: 

                 221     111      43
 
MslI CAynn'nnrTG

Cuts at:      0     283     375 
   Size:        283      92
 
PspOMI G'GGCC_C

Cuts at:      0     221     263     375 
   Size:        221      42     112

  Fragments arranged by size: 

                 221     112      42
 
StuI AGG'CCT

Cuts at:      0     229     375 
   Size:        229     146
 
StyI C'CwwG_G

Cuts at:      0      58     375 
   Size:         58     317
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0      39      71     375 
   Size:         39      32     304

  Fragments arranged by size: 

                 304      39      32
 
Tth111I GACn'n_nGTC

Cuts at:      0     106     375 
   Size:        106     269
 

 Enzymes that do cut:

     AccI     ApaI     BanI    BanII     BclI Bme1580I    BmgBI     BsaI
    BsaBI    BseYI     BsmI Bsp1286I    BspHI     DrdI     EarI EcoO109I
     MslI   PspOMI     StuI     StyI     TstI  Tth111I

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AcuI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI     AloI    AlwNI    ApaLI     ApoI
     AscI     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI     BbeI
     BbsI    BbvCI     BcgI    BciVI    BfrBI     BglI    BglII     BlpI
     BmrI     BmtI     BplI     BpmI   Bpu10I    BpuEI    BsaAI    BsaHI
    BsaWI    BsaXI    BseRI     BsgI    BsiEI  BsiHKAI    BsiWI    BsmBI
    BspEI    BspMI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII    BssSI
   BstAPI    BstBI   BstEII    BstXI    BstYI  BstZ17I   Bsu36I     BtgI
    BtgZI     BtsI     ClaI    CspCI     DraI   DraIII     EaeI     EagI
     EciI  EcoICRI  Eco57MI    EcoNI    EcoRI    EcoRV     FalI     FseI
     FspI    FspAI    HaeII    Hin4I   HincII  HindIII     HpaI     KasI
     KpnI     MfeI     MluI     MmeI     MscI   MspA1I     NaeI     NarI
     NcoI     NdeI   NgoMIV     NheI     NotI     NruI     NsiI     NspI
     PacI     PasI     PciI    PflMI     PfoI     PmeI     PmlI     PpiI
    PpuMI    PshAI     PsiI    PspXI     PsrI     PstI     PvuI    PvuII
    RsrII     SacI    SacII     SalI    SanDI     SapI     SbfI     ScaI
    SexAI     SfcI     SfiI     SfoI     SgfI    SgrAI     SmaI     SmlI
    SnaBI     SpeI     SphI     SrfI     SspI     SwaI    TaqII     TatI
     XbaI     XcmI     XhoI     XmaI     XmnI     ZraI