Restriction map for
pFN21AE1384
(Linear) (Six-base) MAPSORT of: KIEE1384.seq Check: 1550 from: 1 to: 1794
KIEE1384.orf
With 182 enzymes: SgfI *
July 15, 2010 18:37 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 141 558 640 1045 1624 1794
Size: 141 417 82 405 579 170
Fragments arranged by size:
579 417 405 170 141 82
AflIII A'CryG_T
Cuts at: 0 847 1794
Size: 847 947
AlwNI CAG_nnn'CTG
Cuts at: 0 404 1167 1308 1707 1794
Size: 404 763 141 399 87
Fragments arranged by size:
763 404 399 141 87
ApaI G_GGCC'C
Cuts at: 0 548 777 1130 1794
Size: 548 229 353 664
Fragments arranged by size:
664 548 353 229
ApaLI G'TGCA_C
Cuts at: 0 1109 1695 1794
Size: 1109 586 99
Fragments arranged by size:
1109 586 99
AvaI C'yCGr_G
Cuts at: 0 118 726 1678 1794
Size: 118 608 952 116
Fragments arranged by size:
952 608 118 116
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 731 764 1794
Size: 731 33 1030
Fragments arranged by size:
1030 731 33
BanI G'GyrC_C
Cuts at: 0 699 1743 1794
Size: 699 1044 51
Fragments arranged by size:
1044 699 51
BanII G_rGCy'C
Cuts at: 0 548 616 727 777 1130 1337 1637 1794
Size: 548 68 111 50 353 207 300 157
Fragments arranged by size:
548 353 300 207 157 111 68 50
BbvCI CC'TCA_GC
Cuts at: 0 237 273 1794
Size: 237 36 1521
Fragments arranged by size:
1521 237 36
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 935 969 1483 1517 1794
Size: 935 34 514 34 277
Fragments arranged by size:
935 514 277 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 19 725 732 1465 1794
Size: 19 706 7 733 329
Fragments arranged by size:
733 706 329 19 7
BglII A'GATC_T
Cuts at: 0 1769 1794
Size: 1769 25
BlpI GC'TnA_GC
Cuts at: 0 1283 1794
Size: 1283 511
Bme1580I G_kGCm'C
Cuts at: 0 548 777 1113 1130 1428 1699 1748 1794
Size: 548 229 336 17 298 271 49 46
Fragments arranged by size:
548 336 298 271 229 49 46 17
BmgBI CAC'GTC
Cuts at: 0 1660 1794
Size: 1660 134
BmrI ACTGGGnnnn_n'
Cuts at: 0 349 1794
Size: 349 1445
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 360 542 1261 1336 1351 1753 1794
Size: 360 182 719 75 15 402 41
Fragments arranged by size:
719 402 360 182 75 41 15
Bpu10I CC'TnA_GC
Cuts at: 0 237 273 346 1395 1794
Size: 237 36 73 1049 399
Fragments arranged by size:
1049 399 237 73 36
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 739 1452 1711 1794
Size: 739 713 259 83
Fragments arranged by size:
739 713 259 83
BsaAI yAC'GTr
Cuts at: 0 1614 1794
Size: 1614 180
BsaWI w'CCGG_w
Cuts at: 0 1231 1794
Size: 1231 563
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1213 1243 1794
Size: 1213 30 551
Fragments arranged by size:
1213 551 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 225 234 237 246 1081 1588 1794
Size: 225 9 3 9 835 507 206
Fragments arranged by size:
835 507 225 206 9 9 3
BseYI C'CCAG_C
Cuts at: 0 151 430 446 487 592 598 651 1129
Size: 151 279 16 41 105 6 53 478
Cuts at: 1129 1198 1637 1794
Size: 69 439 157
Fragments arranged by size:
478 439 279 157 151 105 69 53
41 16 6
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 904 1462 1794
Size: 904 558 332
Fragments arranged by size:
904 558 332
BsiEI CG_ry'CG
Cuts at: 0 1542 1794
Size: 1542 252
BsiHKAI G_wGCw'C
Cuts at: 0 1113 1337 1613 1699 1794
Size: 1113 224 276 86 95
Fragments arranged by size:
1113 276 224 95 86
Bsp1286I G_dGCh'C
Cuts at: 0 548 616 727 777 1113 1130 1337 1428
Size: 548 68 111 50 336 17 207 91
Cuts at: 1428 1613 1637 1699 1748 1794
Size: 185 24 62 49 46
Fragments arranged by size:
548 336 207 185 111 91 68 62
50 49 46 24 17
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1232 1794
Size: 1232 562
BsrBI CCG'CTC
Cuts at: 0 350 770 1794
Size: 350 420 1024
Fragments arranged by size:
1024 420 350
BsrFI r'CCGG_y
Cuts at: 0 586 1040 1293 1412 1541 1794
Size: 586 454 253 119 129 253
Fragments arranged by size:
586 454 253 253 129 119
BsrGI T'GTAC_A
Cuts at: 0 1206 1794
Size: 1206 588
BstAPI GCAn_nnn'nTGC
Cuts at: 0 404 1485 1551 1794
Size: 404 1081 66 243
Fragments arranged by size:
1081 404 243 66
BstXI CCAn_nnnn'nTGG
Cuts at: 0 108 835 1794
Size: 108 727 959
Fragments arranged by size:
959 727 108
BstYI r'GATC_y
Cuts at: 0 1268 1769 1794
Size: 1268 501 25
Fragments arranged by size:
1268 501 25
BtgI C'CryG_G
Cuts at: 0 101 1428 1561 1794
Size: 101 1327 133 233
Fragments arranged by size:
1327 233 133 101
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1575 1794
Size: 1575 219
BtsI GCAGTG_nn'
Cuts at: 0 140 1133 1426 1794
Size: 140 993 293 368
Fragments arranged by size:
993 368 293 140
EaeI y'GGCC_r
Cuts at: 0 110 474 1794
Size: 110 364 1320
Fragments arranged by size:
1320 364 110
EarI CTCTTCn'nnn_
Cuts at: 0 1342 1794
Size: 1342 452
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1276 1336 1794
Size: 1276 60 458
Fragments arranged by size:
1276 458 60
EcoICRI GAG'CTC
Cuts at: 0 1335 1794
Size: 1335 459
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 141 360 542 558 640 1045 1261 1336
Size: 141 219 182 16 82 405 216 75
Cuts at: 1336 1351 1624 1753 1794
Size: 15 273 129 41
Fragments arranged by size:
405 273 219 216 182 141 129 82
75 41 16 15
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1467 1794
Size: 1467 327
EcoO109I rG'GnC_Cy
Cuts at: 0 46 366 545 773 774 1126 1305 1472
Size: 46 320 179 228 1 352 179 167
Cuts at: 1472 1704 1794
Size: 232 90
Fragments arranged by size:
352 320 232 228 179 179 167 90
46 1
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 885 1404 1794
Size: 885 519 390
Fragments arranged by size:
885 519 390
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 355 387 1213 1245 1794
Size: 355 32 826 32 549
Fragments arranged by size:
826 549 355 32 32
MscI TGG'CCA
Cuts at: 0 112 1794
Size: 112 1682
MslI CAynn'nnrTG
Cuts at: 0 166 836 1794
Size: 166 670 958
Fragments arranged by size:
958 670 166
MspA1I CmG'CkG
Cuts at: 0 133 210 528 651 797 953 1563 1598
Size: 133 77 318 123 146 156 610 35
Cuts at: 1598 1621 1649 1794
Size: 23 28 145
Fragments arranged by size:
610 318 156 146 145 133 123 77
35 28 23
NaeI GCC'GGC
Cuts at: 0 1042 1794
Size: 1042 752
NcoI C'CATG_G
Cuts at: 0 101 1794
Size: 101 1693
NgoMIV G'CCGG_C
Cuts at: 0 1040 1794
Size: 1040 754
NspI r_CATG'y
Cuts at: 0 851 1794
Size: 851 943
PasI CC'CwG_GG
Cuts at: 0 458 1701 1794
Size: 458 1243 93
Fragments arranged by size:
1243 458 93
PciI A'CATG_T
Cuts at: 0 847 1794
Size: 847 947
PflMI CCAn_nnn'nTGG
Cuts at: 0 651 1167 1794
Size: 651 516 627
Fragments arranged by size:
651 627 516
PfoI T'CCnGG_A
Cuts at: 0 42 1160 1328 1794
Size: 42 1118 168 466
Fragments arranged by size:
1118 466 168 42
PmlI CAC'GTG
Cuts at: 0 1614 1794
Size: 1614 180
PpuMI rG'GwC_Cy
Cuts at: 0 46 1305 1472 1794
Size: 46 1259 167 322
Fragments arranged by size:
1259 322 167 46
PspOMI G'GGCC_C
Cuts at: 0 544 773 1126 1794
Size: 544 229 353 668
Fragments arranged by size:
668 544 353 229
PstI C_TGCA'G
Cuts at: 0 1421 1556 1583 1712 1794
Size: 1421 135 27 129 82
Fragments arranged by size:
1421 135 129 82 27
PvuII CAG'CTG
Cuts at: 0 210 528 651 1598 1621 1649 1794
Size: 210 318 123 947 23 28 145
Fragments arranged by size:
947 318 210 145 123 28 23
SacI G_AGCT'C
Cuts at: 0 1337 1794
Size: 1337 457
SacII CC_GC'GG
Cuts at: 0 1564 1794
Size: 1564 230
SanDI GG'GwC_CC
Cuts at: 0 46 1794
Size: 46 1748
SapI GCTCTTCn'nnn_
Cuts at: 0 1342 1794
Size: 1342 452
SfcI C'TryA_G
Cuts at: 0 1417 1552 1579 1708 1794
Size: 1417 135 27 129 86
Fragments arranged by size:
1417 135 129 86 27
SmlI C'TyrA_G
Cuts at: 0 718 1467 1726 1794
Size: 718 749 259 68
Fragments arranged by size:
749 718 259 68
StuI AGG'CCT
Cuts at: 0 506 1400 1794
Size: 506 894 394
Fragments arranged by size:
894 506 394
StyI C'CwwG_G
Cuts at: 0 101 758 1794
Size: 101 657 1036
Fragments arranged by size:
1036 657 101
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 658 717 1714 1770 1794
Size: 658 59 997 56 24
Fragments arranged by size:
997 658 59 56 24
TatI w'GTAC_w
Cuts at: 0 899 1206 1794
Size: 899 307 588
Fragments arranged by size:
899 588 307
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 19 105 600 648 1794
Size: 19 86 495 48 1146
Fragments arranged by size:
1146 495 86 48 19
XmnI GAAnn'nnTTC
Cuts at: 0 625 1794
Size: 625 1169
Enzymes that do cut:
AcuI AflIII AlwNI ApaI ApaLI AvaI BaeI BanI
BanII BbvCI BcgI BciVI BglII BlpI Bme1580I BmgBI
BmrI BpmI Bpu10I BpuEI BsaAI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI Bsp1286I BspMI BsrBI BsrFI
BsrGI BstAPI BstXI BstYI BtgI BtgZI BtsI EaeI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I FalI Hin4I
MscI MslI MspA1I NaeI NcoI NgoMIV NspI PasI
PciI PflMI PfoI PmlI PpuMI PspOMI PstI PvuII
SacI SacII SanDI SapI SfcI SmlI StuI StyI
TaqII TatI XcmI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AgeI
AhdI AleI AlfI AloI ApoI AscI AseI AsiSI
AvrII BamHI BbeI BbsI BclI BfrBI BglI BmtI
BplI BsaI BsaBI BsaHI BsiWI BsmI BsmBI BspEI
BspHI BsrDI BssHII BssSI BstBI BstEII BstZ17I Bsu36I
ClaI CspCI DraI DraIII DrdI EagI EcoRI EcoRV
FseI FspI FspAI HaeII HincII HindIII HpaI KasI
KpnI MfeI MluI MmeI NarI NdeI NheI NotI
NruI NsiI PacI PmeI PpiI PshAI PsiI PspXI
PsrI PvuI RsrII SalI SbfI ScaI SexAI SfiI
SfoI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI
SspI SwaI TstI Tth111I XbaI XhoI XmaI ZraI