Restriction map for
pFN21AE1383
(Linear) (Six-base) MAPSORT of: KIEE1383.seq Check: 9450 from: 1 to: 2067
KIEE1383.orf
With 182 enzymes: SgfI *
July 15, 2010 18:37 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 408 2067
Size: 408 1659
AccI GT'mk_AC
Cuts at: 0 941 2067
Size: 941 1126
Acc65I G'GTAC_C
Cuts at: 0 191 2067
Size: 191 1876
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 555 973 2067
Size: 555 418 1094
Fragments arranged by size:
1094 555 418
AflIII A'CryG_T
Cuts at: 0 479 776 1691 2067
Size: 479 297 915 376
Fragments arranged by size:
915 479 376 297
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 142 1873 2067
Size: 142 1731 194
Fragments arranged by size:
1731 194 142
AlwNI CAG_nnn'CTG
Cuts at: 0 186 874 1570 1797 1968 2067
Size: 186 688 696 227 171 99
Fragments arranged by size:
696 688 227 186 171 99
ApoI r'AATT_y
Cuts at: 0 250 490 2067
Size: 250 240 1577
Fragments arranged by size:
1577 250 240
AvaI C'yCGr_G
Cuts at: 0 103 1762 2067
Size: 103 1659 305
Fragments arranged by size:
1659 305 103
BanI G'GyrC_C
Cuts at: 0 191 420 620 1054 1980 2031 2067
Size: 191 229 200 434 926 51 36
Fragments arranged by size:
926 434 229 200 191 51 36
BanII G_rGCy'C
Cuts at: 0 110 545 1332 1351 1514 2067
Size: 110 435 787 19 163 553
Fragments arranged by size:
787 553 435 163 110 19
BbsI GAAGACnn'nnnn_
Cuts at: 0 855 1374 1566 1658 2067
Size: 855 519 192 92 409
Fragments arranged by size:
855 519 409 192 92
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 194 228 1093 1127 2067
Size: 194 34 865 34 940
Fragments arranged by size:
940 865 194 34 34
BclI T'GATC_A
Cuts at: 0 1503 2067
Size: 1503 564
BfrBI ATG'CAT
Cuts at: 0 1683 2067
Size: 1683 384
BglI GCCn_nnn'nGGC
Cuts at: 0 70 2067
Size: 70 1997
BglII A'GATC_T
Cuts at: 0 170 320 2067
Size: 170 150 1747
Fragments arranged by size:
1747 170 150
BlpI GC'TnA_GC
Cuts at: 0 799 2023 2067
Size: 799 1224 44
Fragments arranged by size:
1224 799 44
Bme1580I G_kGCm'C
Cuts at: 0 1057 1163 1985 2067
Size: 1057 106 822 82
Fragments arranged by size:
1057 822 106 82
BmgBI CAC'GTC
Cuts at: 0 30 480 2067
Size: 30 450 1587
Fragments arranged by size:
1587 450 30
BmrI ACTGGGnnnn_n'
Cuts at: 0 752 1716 2067
Size: 752 964 351
Fragments arranged by size:
964 752 351
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 31 160 247 304 934 1294 1627 2067
Size: 31 129 87 57 630 360 333 440
Fragments arranged by size:
630 440 360 333 129 87 57 31
Bpu10I CC'TnA_GC
Cuts at: 0 1331 1913 2067
Size: 1331 582 154
Fragments arranged by size:
1331 582 154
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1849 2067
Size: 1849 218
BsaI GGTCTCn'nnnn_
Cuts at: 0 691 2067
Size: 691 1376
BsaAI yAC'GTr
Cuts at: 0 990 2067
Size: 990 1077
BsaHI Gr'CG_yC
Cuts at: 0 1442 1946 2067
Size: 1442 504 121
Fragments arranged by size:
1442 504 121
BsaWI w'CCGG_w
Cuts at: 0 426 1074 1301 2067
Size: 426 648 227 766
Fragments arranged by size:
766 648 426 227
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 832 862 1234 1264 1441 1471 1804 1834
Size: 832 30 372 30 177 30 333 30
Cuts at: 1834 1837 1867 2067
Size: 3 30 200
Fragments arranged by size:
832 372 333 200 177 30 30 30
30 30 3
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 244 274 307 310 1483 1825 1843 1852
Size: 244 30 33 3 1173 342 18 9
Cuts at: 1852 2067
Size: 215
Fragments arranged by size:
1173 342 244 215 33 30 18 9
3
BseYI C'CCAG_C
Cuts at: 0 109 186 851 1638 1961 2067
Size: 109 77 665 787 323 106
Fragments arranged by size:
787 665 323 109 106 77
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 820 1942 1993 2067
Size: 820 1122 51 74
Fragments arranged by size:
1122 820 74 51
BsiEI CG_ry'CG
Cuts at: 0 74 896 1209 1439 2067
Size: 74 822 313 230 628
Fragments arranged by size:
822 628 313 230 74
BsiHKAI G_wGCw'C
Cuts at: 0 545 920 1514 2067
Size: 545 375 594 553
Fragments arranged by size:
594 553 545 375
BsmBI CGTCTCn'nnnn_
Cuts at: 0 280 1825 2067
Size: 280 1545 242
Fragments arranged by size:
1545 280 242
Bsp1286I G_dGCh'C
Cuts at: 0 110 545 920 1057 1163 1332 1351 1514
Size: 110 435 375 137 106 169 19 163
Cuts at: 1514 1985 2067
Size: 471 82
Fragments arranged by size:
471 435 375 169 163 137 110 106
82 19
BspEI T'CCGG_A
Cuts at: 0 426 1074 2067
Size: 426 648 993
Fragments arranged by size:
993 648 426
BspHI T'CATG_A
Cuts at: 0 338 818 2067
Size: 338 480 1249
Fragments arranged by size:
1249 480 338
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 203 408 842 2067
Size: 203 205 434 1225
Fragments arranged by size:
1225 434 205 203
BsrBI CCG'CTC
Cuts at: 0 1361 1545 2067
Size: 1361 184 522
Fragments arranged by size:
1361 522 184
BsrFI r'CCGG_y
Cuts at: 0 69 1601 2067
Size: 69 1532 466
Fragments arranged by size:
1532 466 69
BssHII G'CGCG_C
Cuts at: 0 76 78 2067
Size: 76 2 1989
Fragments arranged by size:
1989 76 2
BssSI C'ACGA_G
Cuts at: 0 1418 2067
Size: 1418 649
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1673 1797 1971 1980 2067
Size: 1673 124 174 9 87
Fragments arranged by size:
1673 174 124 87 9
BstEII G'GTnAC_C
Cuts at: 0 156 2067
Size: 156 1911
BstXI CCAn_nnnn'nTGG
Cuts at: 0 243 2067
Size: 243 1824
BstYI r'GATC_y
Cuts at: 0 170 320 429 995 1814 1868 2067
Size: 170 150 109 566 819 54 199
Fragments arranged by size:
819 566 199 170 150 109 54
BtgI C'CryG_G
Cuts at: 0 47 1059 1229 1805 1872 2012 2067
Size: 47 1012 170 576 67 140 55
Fragments arranged by size:
1012 576 170 140 67 55 47
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 565 2067
Size: 565 1502
BtsI GCAGTG_nn'
Cuts at: 0 126 1116 1903 1930 2058 2067
Size: 126 990 787 27 128 9
Fragments arranged by size:
990 787 128 126 27 9
EaeI y'GGCC_r
Cuts at: 0 23 44 71 893 984 1226 1436 2067
Size: 23 21 27 822 91 242 210 631
Fragments arranged by size:
822 631 242 210 91 27 23 21
EagI C'GGCC_G
Cuts at: 0 71 893 1436 2067
Size: 71 822 543 631
Fragments arranged by size:
822 631 543 71
EarI CTCTTCn'nnn_
Cuts at: 0 49 205 370 439 446 1147 2067
Size: 49 156 165 69 7 701 920
Fragments arranged by size:
920 701 165 156 69 49 7
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 19 2067
Size: 19 2048
EcoICRI GAG'CTC
Cuts at: 0 543 1512 2067
Size: 543 969 555
Fragments arranged by size:
969 555 543
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 31 160 247 304 555 934 973 1294
Size: 31 129 87 57 251 379 39 321
Cuts at: 1294 1627 2067
Size: 333 440
Fragments arranged by size:
440 379 333 321 251 129 87 57
39 31
EcoNI CCTnn'n_nnAGG
Cuts at: 0 229 1087 1273 1666 2067
Size: 229 858 186 393 401
Fragments arranged by size:
858 401 393 229 186
EcoO109I rG'GnC_Cy
Cuts at: 0 239 831 948 1080 1785 2067
Size: 239 592 117 132 705 282
Fragments arranged by size:
705 592 282 239 132 117
EcoRI G'AATT_C
Cuts at: 0 250 2067
Size: 250 1817
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 819 2067
Size: 819 1248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1108 1140 1234 1266 1441 1473 1804 1836
Size: 1108 32 94 32 175 32 331 32
Cuts at: 1836 2067
Size: 231
Fragments arranged by size:
1108 331 231 175 94 32 32 32
32
HincII GTy'rAC
Cuts at: 0 1299 2067
Size: 1299 768
KpnI G_GTAC'C
Cuts at: 0 195 2067
Size: 195 1872
MluI A'CGCG_T
Cuts at: 0 776 2067
Size: 776 1291
MscI TGG'CCA
Cuts at: 0 46 986 2067
Size: 46 940 1081
Fragments arranged by size:
1081 940 46
MslI CAynn'nnrTG
Cuts at: 0 682 2067
Size: 682 1385
MspA1I CmG'CkG
Cuts at: 0 1407 1638 1731 1874 1968 1977 2037 2067
Size: 1407 231 93 143 94 9 60 30
Fragments arranged by size:
1407 231 143 94 93 60 30 9
NaeI GCC'GGC
Cuts at: 0 71 2067
Size: 71 1996
NcoI C'CATG_G
Cuts at: 0 47 1805 2067
Size: 47 1758 262
Fragments arranged by size:
1758 262 47
NgoMIV G'CCGG_C
Cuts at: 0 69 2067
Size: 69 1998
NsiI A_TGCA'T
Cuts at: 0 1685 2067
Size: 1685 382
NspI r_CATG'y
Cuts at: 0 394 771 882 924 1140 1695 2067
Size: 394 377 111 42 216 555 372
Fragments arranged by size:
555 394 377 372 216 111 42
PasI CC'CwG_GG
Cuts at: 0 1662 2067
Size: 1662 405
PciI A'CATG_T
Cuts at: 0 1691 2067
Size: 1691 376
PflMI CCAn_nnn'nTGG
Cuts at: 0 186 1824 1968 2067
Size: 186 1638 144 99
Fragments arranged by size:
1638 186 144 99
PfoI T'CCnGG_A
Cuts at: 0 809 2067
Size: 809 1258
PmlI CAC'GTG
Cuts at: 0 990 2067
Size: 990 1077
PpuMI rG'GwC_Cy
Cuts at: 0 831 948 1080 2067
Size: 831 117 132 987
Fragments arranged by size:
987 831 132 117
PspXI vC'TCGA_Gb
Cuts at: 0 1762 2067
Size: 1762 305
PstI C_TGCA'G
Cuts at: 0 1088 1289 2067
Size: 1088 201 778
Fragments arranged by size:
1088 778 201
PvuII CAG'CTG
Cuts at: 0 1638 1968 2067
Size: 1638 330 99
Fragments arranged by size:
1638 330 99
SacI G_AGCT'C
Cuts at: 0 545 1514 2067
Size: 545 969 553
Fragments arranged by size:
969 553 545
SacII CC_GC'GG
Cuts at: 0 1875 2067
Size: 1875 192
SapI GCTCTTCn'nnn_
Cuts at: 0 49 205 1147 2067
Size: 49 156 942 920
Fragments arranged by size:
942 920 156 49
SfcI C'TryA_G
Cuts at: 0 1084 1285 2067
Size: 1084 201 782
Fragments arranged by size:
1084 782 201
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 70 2067
Size: 70 1997
SmlI C'TyrA_G
Cuts at: 0 1762 1864 2067
Size: 1762 102 203
Fragments arranged by size:
1762 203 102
SphI G_CATG'C
Cuts at: 0 1140 2067
Size: 1140 927
StyI C'CwwG_G
Cuts at: 0 47 242 396 1805 2067
Size: 47 195 154 1409 262
Fragments arranged by size:
1409 262 195 154 47
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 608 1072 2067
Size: 608 464 995
Fragments arranged by size:
995 608 464
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1837 1869 2067
Size: 1837 32 198
Fragments arranged by size:
1837 198 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 243 660 969 2067
Size: 243 417 309 1098
Fragments arranged by size:
1098 417 309 243
XhoI C'TCGA_G
Cuts at: 0 1762 2067
Size: 1762 305
XmnI GAAnn'nnTTC
Cuts at: 0 568 1885 2067
Size: 568 1317 182
Fragments arranged by size:
1317 568 182
Enzymes that do cut:
AarI AccI Acc65I AcuI AflIII AlfI AlwNI ApoI
AvaI BanI BanII BbsI BcgI BclI BfrBI BglI
BglII BlpI Bme1580I BmgBI BmrI BpmI Bpu10I BpuEI
BsaI BsaAI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrFI BssHII BssSI BstAPI BstEII BstXI BstYI BtgI
BtgZI BtsI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FalI Hin4I HincII KpnI MluI
MscI MslI MspA1I NaeI NcoI NgoMIV NsiI NspI
PasI PciI PflMI PfoI PmlI PpuMI PspXI PstI
PvuII SacI SacII SapI SfcI SfiI SmlI SphI
StyI TaqII TstI XcmI XhoI XmnI
Enzymes that do not cut:
AatII AclI AfeI AflII AgeI AhdI AleI AloI
ApaI ApaLI AscI AseI AsiSI AvrII BaeI BamHI
BbeI BbvCI BciVI BmtI BplI BsaBI BsiWI BsmI
BsrDI BsrGI BstBI BstZ17I Bsu36I ClaI CspCI DraI
DraIII DrdI EcoRV FseI FspI FspAI HaeII HindIII
HpaI KasI MfeI MmeI NarI NdeI NheI NotI
NruI PacI PmeI PpiI PshAI PsiI PspOMI PsrI
PvuI RsrII SalI SanDI SbfI ScaI SexAI SfoI
SgfI SgrAI SmaI SnaBI SpeI SrfI SspI StuI
SwaI TatI Tth111I XbaI XmaI ZraI