(Linear) (Six-base) MAPSORT of: KIEE1382.seq  Check: 7237  from: 1  to: 339



KIEE1382.orf

 With 182 enzymes: SgfI * 

                        July 15, 2010 18:37  ..

AccI GT'mk_AC

Cuts at:      0     302     339 
   Size:        302      37
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0      46     339 
   Size:         46     293
 
AvaI C'yCGr_G

Cuts at:      0     117     206     339 
   Size:        117      89     133

  Fragments arranged by size: 

                 133     117      89
 
BanII G_rGCy'C

Cuts at:      0     118     281     339 
   Size:        118     163      58

  Fragments arranged by size: 

                 163     118      58
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     265     339 
   Size:        265      74
 
BsaI GGTCTCn'nnnn_

Cuts at:      0      67     140     250     339 
   Size:         67      73     110      89

  Fragments arranged by size: 

                 110      89      73      67
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     137     167     339 
   Size:        137      30     172

  Fragments arranged by size: 

                 172     137      30
 
BsiHKAI G_wGCw'C

Cuts at:      0     167     281     339 
   Size:        167     114      58

  Fragments arranged by size: 

                 167     114      58
 
Bsp1286I G_dGCh'C

Cuts at:      0     118     167     281     339 
   Size:        118      49     114      58

  Fragments arranged by size: 

                 118     114      58      49
 
BsrDI GCAATG_nn'

Cuts at:      0     167     339 
   Size:        167     172
 
BsrFI r'CCGG_y

Cuts at:      0     194     339 
   Size:        194     145
 
EaeI y'GGCC_r

Cuts at:      0     176     339 
   Size:        176     163
 
EcoICRI GAG'CTC

Cuts at:      0     279     339 
   Size:        279      60
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     265     339 
   Size:        265      74
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0      21     339 
   Size:         21     318
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     247     279     339 
   Size:        247      32      60

  Fragments arranged by size: 

                 247      60      32
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     275     339 
   Size:        275      64
 
NaeI GCC'GGC

Cuts at:      0     196     339 
   Size:        196     143
 
NgoMIV G'CCGG_C

Cuts at:      0     194     339 
   Size:        194     145
 
PstI C_TGCA'G

Cuts at:      0      22     339 
   Size:         22     317
 
SacI G_AGCT'C

Cuts at:      0     281     339 
   Size:        281      58
 
SexAI A'CCwGG_T

Cuts at:      0     149     339 
   Size:        149     190
 
SfcI C'TryA_G

Cuts at:      0      18     303     339 
   Size:         18     285      36

  Fragments arranged by size: 

                 285      36      18
 
SmaI CCC'GGG

Cuts at:      0     119     339 
   Size:        119     220
 
SrfI GCCC'GGGC

Cuts at:      0     119     339 
   Size:        119     220
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     274     339 
   Size:        274      65
 
TatI w'GTAC_w

Cuts at:      0     182     339 
   Size:        182     157
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     137     169     339 
   Size:        137      32     170

  Fragments arranged by size: 

                 170     137      32
 
Tth111I GACn'n_nGTC

Cuts at:      0     261     339 
   Size:        261      78
 
XmaI C'CCGG_G

Cuts at:      0     117     339 
   Size:        117     222
 

 Enzymes that do cut:

     AccI     AlfI     AvaI    BanII     BpmI     BsaI    BsaXI  BsiHKAI
 Bsp1286I    BsrDI    BsrFI     EaeI  EcoICRI  Eco57MI    EcoNI    Hin4I
     MmeI     NaeI   NgoMIV     PstI     SacI    SexAI     SfcI     SmaI
     SrfI    TaqII     TatI     TstI  Tth111I     XmaI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AcuI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AloI    AlwNI     ApaI    ApaLI     ApoI
     AscI     AseI    AsiSI    AvrII     BaeI    BamHI     BanI     BbeI
     BbsI    BbvCI     BcgI    BciVI     BclI    BfrBI     BglI    BglII
     BlpI Bme1580I    BmgBI     BmrI     BmtI     BplI   Bpu10I    BpuEI
    BsaAI    BsaBI    BsaHI    BsaWI    BseRI    BseYI     BsgI    BsiEI
    BsiWI     BsmI    BsmBI    BspEI    BspHI    BspMI    BsrBI    BsrGI
   BssHII    BssSI   BstAPI    BstBI   BstEII    BstXI    BstYI  BstZ17I
   Bsu36I     BtgI    BtgZI     BtsI     ClaI    CspCI     DraI   DraIII
     DrdI     EagI     EarI     EciI EcoO109I    EcoRI    EcoRV     FalI
     FseI     FspI    FspAI    HaeII   HincII  HindIII     HpaI     KasI
     KpnI     MfeI     MluI     MscI     MslI   MspA1I     NarI     NcoI
     NdeI     NheI     NotI     NruI     NsiI     NspI     PacI     PasI
     PciI    PflMI     PfoI     PmeI     PmlI     PpiI    PpuMI    PshAI
     PsiI   PspOMI    PspXI     PsrI     PvuI    PvuII    RsrII    SacII
     SalI    SanDI     SapI     SbfI     ScaI     SfiI     SfoI     SgfI
    SgrAI     SmlI    SnaBI     SpeI     SphI     SspI     StuI     StyI
     SwaI     XbaI     XcmI     XhoI     XmnI     ZraI