Restriction map for
pFN21AE1381
(Linear) (Six-base) MAPSORT of: KIEE1381.seq Check: 314 from: 1 to: 2349
KIEE1381.orf
With 182 enzymes: SgfI *
July 15, 2010 18:37 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 786 1077 2349
Size: 786 291 1272
Fragments arranged by size:
1272 786 291
AccI GT'mk_AC
Cuts at: 0 2099 2349
Size: 2099 250
Acc65I G'GTAC_C
Cuts at: 0 881 2349
Size: 881 1468
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 347 637 2288 2349
Size: 347 290 1651 61
Fragments arranged by size:
1651 347 290 61
AflIII A'CryG_T
Cuts at: 0 1287 1682 2349
Size: 1287 395 667
Fragments arranged by size:
1287 667 395
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 337 2349
Size: 337 2012
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 394 426 2349
Size: 394 32 1923
Fragments arranged by size:
1923 394 32
AlwNI CAG_nnn'CTG
Cuts at: 0 309 1194 1215 1494 1835 1913 2349
Size: 309 885 21 279 341 78 436
Fragments arranged by size:
885 436 341 309 279 78 21
ApaI G_GGCC'C
Cuts at: 0 589 704 1181 1227 1255 1422 1429 2064
Size: 589 115 477 46 28 167 7 635
Cuts at: 2064 2349
Size: 285
Fragments arranged by size:
635 589 477 285 167 115 46 28
7
ApoI r'AATT_y
Cuts at: 0 718 2349
Size: 718 1631
AvaI C'yCGr_G
Cuts at: 0 261 452 687 989 1180 1226 1415 2349
Size: 261 191 235 302 191 46 189 934
Fragments arranged by size:
934 302 261 235 191 191 189 46
AvrII C'CTAG_G
Cuts at: 0 696 2349
Size: 696 1653
BamHI G'GATC_C
Cuts at: 0 1899 2349
Size: 1899 450
BanI G'GyrC_C
Cuts at: 0 68 649 881 978 1074 1358 1603 2195
Size: 68 581 232 97 96 284 245 592
Cuts at: 2195 2349
Size: 154
Fragments arranged by size:
592 581 284 245 232 154 97 96
68
BanII G_rGCy'C
Cuts at: 0 31 42 260 376 400 407 559 589
Size: 31 11 218 116 24 7 152 30
Cuts at: 589 704 874 1000 1181 1187 1227 1255 1422
Size: 115 170 126 181 6 40 28 167
Cuts at: 1422 1429 1703 1804 2064 2349
Size: 7 274 101 260 285
Fragments arranged by size:
285 274 260 218 181 170 167 152
126 116 115 101 40 31 30 28
24 11 7 7 6
BbeI G_GCGC'C
Cuts at: 0 1362 2349
Size: 1362 987
BbvCI CC'TCA_GC
Cuts at: 0 247 1397 1828 2349
Size: 247 1150 431 521
Fragments arranged by size:
1150 521 431 247
BglI GCCn_nnn'nGGC
Cuts at: 0 150 204 380 1278 1332 1756 2349
Size: 150 54 176 898 54 424 593
Fragments arranged by size:
898 593 424 176 150 54 54
BlpI GC'TnA_GC
Cuts at: 0 578 1216 1627 2303 2349
Size: 578 638 411 676 46
Fragments arranged by size:
676 638 578 411 46
Bme1580I G_kGCm'C
Cuts at: 0 71 116 589 652 704 1181 1227 1255
Size: 71 45 473 63 52 477 46 28
Cuts at: 1255 1422 1429 1940 2064 2349
Size: 167 7 511 124 285
Fragments arranged by size:
511 477 473 285 167 124 71 63
52 46 45 28 7
BmgBI CAC'GTC
Cuts at: 0 111 2349
Size: 111 2238
BmrI ACTGGGnnnn_n'
Cuts at: 0 74 595 1459 2349
Size: 74 521 864 890
Fragments arranged by size:
890 864 521 74
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 546 2349
Size: 546 1803
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 32 56 336 388 541 573 614 744
Size: 32 24 280 52 153 32 41 130
Cuts at: 744 1315 1690 1717 1736 2349
Size: 571 375 27 19 613
Fragments arranged by size:
613 571 375 280 153 130 52 41
32 32 27 24 19
Bpu10I CC'TnA_GC
Cuts at: 0 247 1397 1450 1658 1828 2349
Size: 247 1150 53 208 170 521
Fragments arranged by size:
1150 521 247 208 170 53
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 617 1306 2349
Size: 617 689 1043
Fragments arranged by size:
1043 689 617
BsaI GGTCTCn'nnnn_
Cuts at: 0 748 2116 2349
Size: 748 1368 233
Fragments arranged by size:
1368 748 233
BsaHI Gr'CG_yC
Cuts at: 0 383 1359 2349
Size: 383 976 990
Fragments arranged by size:
990 976 383
BsaWI w'CCGG_w
Cuts at: 0 652 1077 2349
Size: 652 425 1272
Fragments arranged by size:
1272 652 425
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 394 424 587 617 934 964 1663 1693
Size: 394 30 163 30 317 30 699 30
Cuts at: 1693 2179 2209 2349
Size: 486 30 140
Fragments arranged by size:
699 486 394 317 163 140 30 30
30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 457 549 982 985 1870 2158 2161 2200
Size: 457 92 433 3 885 288 3 39
Cuts at: 2200 2349
Size: 149
Fragments arranged by size:
885 457 433 288 149 92 39 3
3
BseYI C'CCAG_C
Cuts at: 0 43 611 823 955 999 1174 1248 1522
Size: 43 568 212 132 44 175 74 274
Cuts at: 1522 1702 1903 1997 2215 2349
Size: 180 201 94 218 134
Fragments arranged by size:
568 274 218 212 201 180 175 134
132 94 74 44 43
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 223 1168 1828 1899 2349
Size: 223 945 660 71 450
Fragments arranged by size:
945 660 450 223 71
BsiEI CG_ry'CG
Cuts at: 0 154 233 2349
Size: 154 79 2116
Fragments arranged by size:
2116 154 79
BsiHKAI G_wGCw'C
Cuts at: 0 407 559 874 1322 2349
Size: 407 152 315 448 1027
Fragments arranged by size:
1027 448 407 315 152
BsmBI CGTCTCn'nnnn_
Cuts at: 0 354 390 2349
Size: 354 36 1959
Fragments arranged by size:
1959 354 36
Bsp1286I G_dGCh'C
Cuts at: 0 31 42 71 116 260 376 400 407
Size: 31 11 29 45 144 116 24 7
Cuts at: 407 559 589 652 704 874 1000 1181 1187
Size: 152 30 63 52 170 126 181 6
Cuts at: 1187 1227 1255 1322 1422 1429 1703 1804 1940
Size: 40 28 67 100 7 274 101 136
Cuts at: 1940 2064 2349
Size: 124 285
Fragments arranged by size:
285 274 181 170 152 144 136 126
124 116 101 100 67 63 52 45
40 31 30 29 28 24 11 7
7 6
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 786 1077 2349
Size: 786 291 1272
Fragments arranged by size:
1272 786 291
BsrBI CCG'CTC
Cuts at: 0 837 1083 2349
Size: 837 246 1266
Fragments arranged by size:
1266 837 246
BsrDI GCAATG_nn'
Cuts at: 0 1303 1308 2349
Size: 1303 5 1041
Fragments arranged by size:
1303 1041 5
BsrFI r'CCGG_y
Cuts at: 0 1361 2349
Size: 1361 988
BsrGI T'GTAC_A
Cuts at: 0 2024 2349
Size: 2024 325
BssSI C'ACGA_G
Cuts at: 0 274 2349
Size: 274 2075
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1913 2349
Size: 1913 436
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1929 2206 2349
Size: 1929 277 143
Fragments arranged by size:
1929 277 143
BstYI r'GATC_y
Cuts at: 0 1899 2349
Size: 1899 450
Bsu36I CC'TnA_GG
Cuts at: 0 1576 2349
Size: 1576 773
BtgI C'CryG_G
Cuts at: 0 1133 2349
Size: 1133 1216
BtsI GCAGTG_nn'
Cuts at: 0 235 906 1863 1914 2160 2349
Size: 235 671 957 51 246 189
Fragments arranged by size:
957 671 246 235 189 51
EaeI y'GGCC_r
Cuts at: 0 151 230 1931 2349
Size: 151 79 1701 418
Fragments arranged by size:
1701 418 151 79
EagI C'GGCC_G
Cuts at: 0 151 230 2349
Size: 151 79 2119
Fragments arranged by size:
2119 151 79
EarI CTCTTCn'nnn_
Cuts at: 0 2023 2349
Size: 2023 326
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 905 1396 2349
Size: 905 491 953
Fragments arranged by size:
953 905 491
EcoICRI GAG'CTC
Cuts at: 0 405 557 872 2349
Size: 405 152 315 1477
Fragments arranged by size:
1477 405 315 152
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 32 56 336 347 388 541 573 614
Size: 32 24 280 11 41 153 32 41
Cuts at: 614 637 744 1315 1690 1717 1736 2288 2349
Size: 23 107 571 375 27 19 552 61
Fragments arranged by size:
571 552 375 280 153 107 61 41
41 32 32 27 24 23 19 11
EcoNI CCTnn'n_nnAGG
Cuts at: 0 934 1396 1777 2026 2349
Size: 934 462 381 249 323
Fragments arranged by size:
934 462 381 323 249
EcoO109I rG'GnC_Cy
Cuts at: 0 96 306 585 693 700 986 1152 1252
Size: 96 210 279 108 7 286 166 100
Cuts at: 1252 1401 1425 1426 1442 1580 2060 2061 2296
Size: 149 24 1 16 138 480 1 235
Cuts at: 2296 2349
Size: 53
Fragments arranged by size:
480 286 279 235 210 166 149 138
108 100 96 53 24 16 7 1
1
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 747 2349
Size: 747 1602
HaeII r_GCGC'y
Cuts at: 0 183 1055 1216 1362 1529 1824 1848 2349
Size: 183 872 161 146 167 295 24 501
Fragments arranged by size:
872 501 295 183 167 161 146 24
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 934 966 1669 1701 2349
Size: 934 32 703 32 648
Fragments arranged by size:
934 703 648 32 32
HindIII A'AGCT_T
Cuts at: 0 729 2349
Size: 729 1620
KasI G'GCGC_C
Cuts at: 0 1358 2349
Size: 1358 991
KpnI G_GTAC'C
Cuts at: 0 885 2349
Size: 885 1464
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1454 1667 2349
Size: 1454 213 682
Fragments arranged by size:
1454 682 213
MspA1I CmG'CkG
Cuts at: 0 125 150 213 229 474 582 615 855
Size: 125 25 63 16 245 108 33 240
Cuts at: 855 1135 1143 1503 2349
Size: 280 8 360 846
Fragments arranged by size:
846 360 280 245 240 125 108 63
33 25 16 8
NaeI GCC'GGC
Cuts at: 0 1363 2349
Size: 1363 986
NarI GG'CG_CC
Cuts at: 0 1359 2349
Size: 1359 990
NgoMIV G'CCGG_C
Cuts at: 0 1361 2349
Size: 1361 988
NotI GC'GGCC_GC
Cuts at: 0 230 2349
Size: 230 2119
NspI r_CATG'y
Cuts at: 0 246 318 1291 1686 2349
Size: 246 72 973 395 663
Fragments arranged by size:
973 663 395 246 72
PasI CC'CwG_GG
Cuts at: 0 1383 1422 2056 2127 2234 2349
Size: 1383 39 634 71 107 115
Fragments arranged by size:
1383 634 115 107 71 39
PciI A'CATG_T
Cuts at: 0 1287 1682 2349
Size: 1287 395 667
Fragments arranged by size:
1287 667 395
PflMI CCAn_nnn'nTGG
Cuts at: 0 939 2349
Size: 939 1410
PfoI T'CCnGG_A
Cuts at: 0 33 365 1292 2349
Size: 33 332 927 1057
Fragments arranged by size:
1057 927 332 33
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 394 426 2349
Size: 394 32 1923
Fragments arranged by size:
1923 394 32
PpuMI rG'GwC_Cy
Cuts at: 0 96 693 986 1152 1442 1580 2349
Size: 96 597 293 166 290 138 769
Fragments arranged by size:
769 597 293 290 166 138 96
PspOMI G'GGCC_C
Cuts at: 0 585 700 1177 1223 1251 1418 1425 2060
Size: 585 115 477 46 28 167 7 635
Cuts at: 2060 2349
Size: 289
Fragments arranged by size:
635 585 477 289 167 115 46 28
7
PstI C_TGCA'G
Cuts at: 0 212 479 1199 1502 1550 2349
Size: 212 267 720 303 48 799
Fragments arranged by size:
799 720 303 267 212 48
PvuII CAG'CTG
Cuts at: 0 213 615 855 1143 1503 2349
Size: 213 402 240 288 360 846
Fragments arranged by size:
846 402 360 288 240 213
SacI G_AGCT'C
Cuts at: 0 407 559 874 2349
Size: 407 152 315 1475
Fragments arranged by size:
1475 407 315 152
SacII CC_GC'GG
Cuts at: 0 1136 2349
Size: 1136 1213
SanDI GG'GwC_CC
Cuts at: 0 986 1442 2349
Size: 986 456 907
Fragments arranged by size:
986 907 456
SbfI CC_TGCA'GG
Cuts at: 0 1550 2349
Size: 1550 799
SfcI C'TryA_G
Cuts at: 0 208 475 1195 1498 1546 2349
Size: 208 267 720 303 48 803
Fragments arranged by size:
803 720 303 267 208 48
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 150 2349
Size: 150 2199
SfoI GGC'GCC
Cuts at: 0 1360 2349
Size: 1360 989
SmaI CCC'GGG
Cuts at: 0 263 454 689 991 1228 1417 2349
Size: 263 191 235 302 237 189 932
Fragments arranged by size:
932 302 263 237 235 191 189
SmlI C'TyrA_G
Cuts at: 0 632 1321 2349
Size: 632 689 1028
Fragments arranged by size:
1028 689 632
SphI G_CATG'C
Cuts at: 0 318 2349
Size: 318 2031
SrfI GCCC'GGGC
Cuts at: 0 1228 1417 2349
Size: 1228 189 932
Fragments arranged by size:
1228 932 189
StuI AGG'CCT
Cuts at: 0 1552 1948 2349
Size: 1552 396 401
Fragments arranged by size:
1552 401 396
StyI C'CwwG_G
Cuts at: 0 389 696 2349
Size: 389 307 1653
Fragments arranged by size:
1653 389 307
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 268 1031 1136 1536 1876 2349
Size: 268 763 105 400 340 473
Fragments arranged by size:
763 473 400 340 268 105
TatI w'GTAC_w
Cuts at: 0 1679 2024 2349
Size: 1679 345 325
Fragments arranged by size:
1679 345 325
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 587 619 2179 2211 2349
Size: 587 32 1560 32 138
Fragments arranged by size:
1560 587 138 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 421 2349
Size: 421 1928
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 534 1171 2349
Size: 534 637 1178
Fragments arranged by size:
1178 637 534
XmaI C'CCGG_G
Cuts at: 0 261 452 687 989 1226 1415 2349
Size: 261 191 235 302 237 189 934
Fragments arranged by size:
934 302 261 237 235 191 189
Enzymes that do cut:
AarI AccI Acc65I AcuI AflIII AlfI AloI AlwNI
ApaI ApoI AvaI AvrII BamHI BanI BanII BbeI
BbvCI BglI BlpI Bme1580I BmgBI BmrI BplI BpmI
Bpu10I BpuEI BsaI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspMI BsrBI BsrDI
BsrFI BsrGI BssSI BstAPI BstXI BstYI Bsu36I BtgI
BtsI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I FalI HaeII Hin4I HindIII KasI KpnI MmeI
MspA1I NaeI NarI NgoMIV NotI NspI PasI PciI
PflMI PfoI PpiI PpuMI PspOMI PstI PvuII SacI
SacII SanDI SbfI SfcI SfiI SfoI SmaI SmlI
SphI SrfI StuI StyI TaqII TatI TstI Tth111I
XcmI XmaI
Enzymes that do not cut:
AatII AclI AfeI AflII AgeI AhdI AleI ApaLI
AscI AseI AsiSI BaeI BbsI BcgI BciVI BclI
BfrBI BglII BmtI BsaAI BsaBI BsiWI BsmI BspEI
BspHI BssHII BstBI BstEII BstZ17I BtgZI ClaI CspCI
DraI DraIII DrdI EcoRI EcoRV FseI FspI FspAI
HincII HpaI MfeI MluI MscI MslI NcoI NdeI
NheI NruI NsiI PacI PmeI PmlI PshAI PsiI
PspXI PsrI PvuI RsrII SalI SapI ScaI SexAI
SgfI SgrAI SnaBI SpeI SspI SwaI XbaI XhoI
XmnI ZraI