Restriction map for
pFN21AE1380
(Linear) (Six-base) MAPSORT of: KIEE1380.seq Check: 5236 from: 1 to: 1974
KIEE1380.orf
With 182 enzymes: SgfI *
July 15, 2010 18:37 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1233 1974
Size: 1233 741
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 79 1572 1974
Size: 79 1493 402
Fragments arranged by size:
1493 402 79
AflIII A'CryG_T
Cuts at: 0 773 1849 1974
Size: 773 1076 125
Fragments arranged by size:
1076 773 125
AlwNI CAG_nnn'CTG
Cuts at: 0 333 1974
Size: 333 1641
ApaI G_GGCC'C
Cuts at: 0 109 223 463 692 1812 1974
Size: 109 114 240 229 1120 162
Fragments arranged by size:
1120 240 229 162 114 109
ApaLI G'TGCA_C
Cuts at: 0 1143 1318 1974
Size: 1143 175 656
Fragments arranged by size:
1143 656 175
AvaI C'yCGr_G
Cuts at: 0 109 275 390 580 730 1570 1974
Size: 109 166 115 190 150 840 404
Fragments arranged by size:
840 404 190 166 150 115 109
BanI G'GyrC_C
Cuts at: 0 265 1504 1633 1792 1974
Size: 265 1239 129 159 182
Fragments arranged by size:
1239 265 182 159 129
BanII G_rGCy'C
Cuts at: 0 109 223 389 463 692 1297 1812 1874
Size: 109 114 166 74 229 605 515 62
Cuts at: 1874 1974
Size: 100
Fragments arranged by size:
605 515 229 166 114 109 100 74
62
BbsI GAAGACnn'nnnn_
Cuts at: 0 306 1695 1974
Size: 306 1389 279
Fragments arranged by size:
1389 306 279
BbvCI CC'TCA_GC
Cuts at: 0 55 1366 1639 1974
Size: 55 1311 273 335
Fragments arranged by size:
1311 335 273 55
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1740 1774 1974
Size: 1740 34 200
Fragments arranged by size:
1740 200 34
BclI T'GATC_A
Cuts at: 0 1259 1974
Size: 1259 715
BglII A'GATC_T
Cuts at: 0 809 839 1974
Size: 809 30 1135
Fragments arranged by size:
1135 809 30
BlpI GC'TnA_GC
Cuts at: 0 479 1974
Size: 479 1495
Bme1580I G_kGCm'C
Cuts at: 0 109 223 463 692 1147 1322 1812 1974
Size: 109 114 240 229 455 175 490 162
Fragments arranged by size:
490 455 240 229 175 162 114 109
BmrI ACTGGGnnnn_n'
Cuts at: 0 523 1974
Size: 523 1451
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 226 229 253 310 584 842 966 1212
Size: 226 3 24 57 274 258 124 246
Cuts at: 1212 1366 1447 1974
Size: 154 81 527
Fragments arranged by size:
527 274 258 246 226 154 124 81
57 24 3
Bpu10I CC'TnA_GC
Cuts at: 0 55 164 1366 1639 1974
Size: 55 109 1202 273 335
Fragments arranged by size:
1202 335 273 109 55
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 460 872 1661 1974
Size: 460 412 789 313
Fragments arranged by size:
789 460 412 313
BsaI GGTCTCn'nnnn_
Cuts at: 0 150 1679 1974
Size: 150 1529 295
Fragments arranged by size:
1529 295 150
BsaAI yAC'GTr
Cuts at: 0 774 1974
Size: 774 1200
BsaHI Gr'CG_yC
Cuts at: 0 791 1097 1974
Size: 791 306 877
Fragments arranged by size:
877 791 306
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1017 1047 1364 1394 1493 1523 1974
Size: 1017 30 317 30 99 30 451
Fragments arranged by size:
1017 451 317 99 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 496 1022 1588 1890 1974
Size: 496 526 566 302 84
Fragments arranged by size:
566 526 496 302 84
BseYI C'CCAG_C
Cuts at: 0 1174 1353 1776 1858 1974
Size: 1174 179 423 82 116
Fragments arranged by size:
1174 423 179 116 82
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 331 1444 1629 1808 1974
Size: 331 1113 185 179 166
Fragments arranged by size:
1113 331 185 179 166
BsiEI CG_ry'CG
Cuts at: 0 592 686 1974
Size: 592 94 1288
Fragments arranged by size:
1288 592 94
BsiHKAI G_wGCw'C
Cuts at: 0 1147 1297 1322 1939 1948 1974
Size: 1147 150 25 617 9 26
Fragments arranged by size:
1147 617 150 26 25 9
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1452 1974
Size: 1452 522
Bsp1286I G_dGCh'C
Cuts at: 0 109 223 389 463 692 1147 1297 1322
Size: 109 114 166 74 229 455 150 25
Cuts at: 1322 1812 1874 1939 1948 1974
Size: 490 62 65 9 26
Fragments arranged by size:
490 455 229 166 150 114 109 74
65 62 26 25 9
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1233 1416 1974
Size: 1233 183 558
Fragments arranged by size:
1233 558 183
BsrDI GCAATG_nn'
Cuts at: 0 1272 1974
Size: 1272 702
BsrFI r'CCGG_y
Cuts at: 0 637 1630 1974
Size: 637 993 344
Fragments arranged by size:
993 637 344
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1952 1974
Size: 1952 22
BstEII G'GTnAC_C
Cuts at: 0 372 1576 1974
Size: 372 1204 398
Fragments arranged by size:
1204 398 372
BstXI CCAn_nnnn'nTGG
Cuts at: 0 930 1107 1968 1974
Size: 930 177 861 6
Fragments arranged by size:
930 861 177 6
BstYI r'GATC_y
Cuts at: 0 434 735 809 839 1974
Size: 434 301 74 30 1135
Fragments arranged by size:
1135 434 301 74 30
Bsu36I CC'TnA_GG
Cuts at: 0 661 1496 1974
Size: 661 835 478
Fragments arranged by size:
835 661 478
BtgI C'CryG_G
Cuts at: 0 6 1508 1874 1974
Size: 6 1502 366 100
Fragments arranged by size:
1502 366 100 6
BtsI GCAGTG_nn'
Cuts at: 0 315 343 1224 1320 1817 1974
Size: 315 28 881 96 497 157
Fragments arranged by size:
881 497 315 157 96 28
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 51 86 332 367 1974
Size: 51 35 246 35 1607
Fragments arranged by size:
1607 246 51 35 35
DrdI GACnn_nn'nnGTC
Cuts at: 0 1207 1687 1974
Size: 1207 480 287
Fragments arranged by size:
1207 480 287
EaeI y'GGCC_r
Cuts at: 0 41 589 635 639 683 956 1532 1974
Size: 41 548 46 4 44 273 576 442
Fragments arranged by size:
576 548 442 273 46 44 41 4
EagI C'GGCC_G
Cuts at: 0 589 683 1974
Size: 589 94 1291
Fragments arranged by size:
1291 589 94
EarI CTCTTCn'nnn_
Cuts at: 0 205 1974
Size: 205 1769
EcoICRI GAG'CTC
Cuts at: 0 1295 1974
Size: 1295 679
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 79 226 229 253 310 584 842 966
Size: 79 147 3 24 57 274 258 124
Cuts at: 966 1212 1366 1447 1572 1974
Size: 246 154 81 125 402
Fragments arranged by size:
402 274 258 246 154 147 125 124
81 79 57 24 3
EcoNI CCTnn'n_nnAGG
Cuts at: 0 901 1499 1974
Size: 901 598 475
Fragments arranged by size:
901 598 475
EcoO109I rG'GnC_Cy
Cuts at: 0 106 168 330 459 460 666 689 763
Size: 106 62 162 129 1 206 23 74
Cuts at: 763 1170 1492 1808 1809 1974
Size: 407 322 316 1 165
Fragments arranged by size:
407 322 316 206 165 162 129 106
74 62 23 1 1
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1227 1974
Size: 1227 747
FseI GG_CCGG'CC
Cuts at: 0 641 1974
Size: 641 1333
FspI TGC'GCA
Cuts at: 0 726 1133 1216 1974
Size: 726 407 83 758
Fragments arranged by size:
758 726 407 83
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 400 432 1017 1049 1327 1359 1659 1691
Size: 400 32 585 32 278 32 300 32
Cuts at: 1691 1974
Size: 283
Fragments arranged by size:
585 400 300 283 278 32 32 32
32
HincII GTy'rAC
Cuts at: 0 399 1974
Size: 399 1575
MluI A'CGCG_T
Cuts at: 0 1849 1974
Size: 1849 125
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 534 1415 1974
Size: 534 881 559
Fragments arranged by size:
881 559 534
MscI TGG'CCA
Cuts at: 0 43 958 1974
Size: 43 915 1016
Fragments arranged by size:
1016 915 43
MslI CAynn'nnrTG
Cuts at: 0 1243 1974
Size: 1243 731
MspA1I CmG'CkG
Cuts at: 0 8 47 231 451 954 986 1178 1232
Size: 8 39 184 220 503 32 192 54
Cuts at: 1232 1308 1353 1475 1643 1974
Size: 76 45 122 168 331
Fragments arranged by size:
503 331 220 192 184 168 122 76
54 45 39 32 8
NaeI GCC'GGC
Cuts at: 0 639 1974
Size: 639 1335
NgoMIV G'CCGG_C
Cuts at: 0 637 1974
Size: 637 1337
NotI GC'GGCC_GC
Cuts at: 0 589 1974
Size: 589 1385
NspI r_CATG'y
Cuts at: 0 549 807 1242 1974
Size: 549 258 435 732
Fragments arranged by size:
732 549 435 258
PasI CC'CwG_GG
Cuts at: 0 333 525 1974
Size: 333 192 1449
Fragments arranged by size:
1449 333 192
PflMI CCAn_nnn'nTGG
Cuts at: 0 333 567 1974
Size: 333 234 1407
Fragments arranged by size:
1407 333 234
PfoI T'CCnGG_A
Cuts at: 0 203 1974
Size: 203 1771
PpuMI rG'GwC_Cy
Cuts at: 0 666 763 1170 1974
Size: 666 97 407 804
Fragments arranged by size:
804 666 407 97
PspOMI G'GGCC_C
Cuts at: 0 105 219 459 688 1808 1974
Size: 105 114 240 229 1120 166
Fragments arranged by size:
1120 240 229 166 114 105
PspXI vC'TCGA_Gb
Cuts at: 0 580 1974
Size: 580 1394
PstI C_TGCA'G
Cuts at: 0 52 761 1004 1974
Size: 52 709 243 970
Fragments arranged by size:
970 709 243 52
PvuII CAG'CTG
Cuts at: 0 47 231 451 986 1178 1232 1353 1475
Size: 47 184 220 535 192 54 121 122
Cuts at: 1475 1643 1974
Size: 168 331
Fragments arranged by size:
535 331 220 192 184 168 122 121
54 47
SacI G_AGCT'C
Cuts at: 0 1297 1974
Size: 1297 677
SacII CC_GC'GG
Cuts at: 0 9 1974
Size: 9 1965
SanDI GG'GwC_CC
Cuts at: 0 763 1170 1974
Size: 763 407 804
Fragments arranged by size:
804 763 407
SbfI CC_TGCA'GG
Cuts at: 0 761 1974
Size: 761 1213
SfcI C'TryA_G
Cuts at: 0 48 757 1000 1974
Size: 48 709 243 974
Fragments arranged by size:
974 709 243 48
SmaI CCC'GGG
Cuts at: 0 392 732 1974
Size: 392 340 1242
Fragments arranged by size:
1242 392 340
SmlI C'TyrA_G
Cuts at: 0 439 580 887 1676 1974
Size: 439 141 307 789 298
Fragments arranged by size:
789 439 307 298 141
SphI G_CATG'C
Cuts at: 0 549 1242 1974
Size: 549 693 732
Fragments arranged by size:
732 693 549
StuI AGG'CCT
Cuts at: 0 893 1387 1757 1974
Size: 893 494 370 217
Fragments arranged by size:
893 494 370 217
StyI C'CwwG_G
Cuts at: 0 605 1037 1535 1974
Size: 605 432 498 439
Fragments arranged by size:
605 498 439 432
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1954 1974
Size: 1954 20
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1364 1396 1493 1525 1837 1869 1974
Size: 1364 32 97 32 312 32 105
Fragments arranged by size:
1364 312 105 97 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 1671 1974
Size: 1671 303
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 564 1965 1974
Size: 564 1401 9
Fragments arranged by size:
1401 564 9
XhoI C'TCGA_G
Cuts at: 0 580 1974
Size: 580 1394
XmaI C'CCGG_G
Cuts at: 0 390 730 1974
Size: 390 340 1244
Fragments arranged by size:
1244 390 340
Enzymes that do cut:
AarI AcuI AflIII AlwNI ApaI ApaLI AvaI BanI
BanII BbsI BbvCI BcgI BclI BglII BlpI Bme1580I
BmrI BpmI Bpu10I BpuEI BsaI BsaAI BsaHI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspMI
BsrDI BsrFI BstAPI BstEII BstXI BstYI Bsu36I BtgI
BtsI CspCI DrdI EaeI EagI EarI EcoICRI Eco57MI
EcoNI EcoO109I FalI FseI FspI Hin4I HincII MluI
MmeI MscI MslI MspA1I NaeI NgoMIV NotI NspI
PasI PflMI PfoI PpuMI PspOMI PspXI PstI PvuII
SacI SacII SanDI SbfI SfcI SmaI SmlI SphI
StuI StyI TaqII TstI Tth111I XcmI XhoI XmaI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflII AgeI AhdI
AleI AlfI AloI ApoI AscI AseI AsiSI AvrII
BaeI BamHI BbeI BciVI BfrBI BglI BmgBI BmtI
BplI BsaBI BsaWI BsiWI BsmI BspEI BspHI BsrBI
BsrGI BssHII BssSI BstBI BstZ17I BtgZI ClaI DraI
DraIII EciI EcoRI EcoRV FspAI HaeII HindIII HpaI
KasI KpnI MfeI NarI NcoI NdeI NheI NruI
NsiI PacI PciI PmeI PmlI PpiI PshAI PsiI
PsrI PvuI RsrII SalI SapI ScaI SexAI SfiI
SfoI SgfI SgrAI SnaBI SpeI SrfI SspI SwaI
TatI XbaI XmnI ZraI