Restriction map for
pFN21AE1377
(Linear) (Six-base) MAPSORT of: KIEE1377.seq Check: 609 from: 1 to: 1578
KIEE1377.orf
With 182 enzymes: SgfI *
July 15, 2010 18:37 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 69 169 182 440 693 1380 1578
Size: 69 100 13 258 253 687 198
Fragments arranged by size:
687 258 253 198 100 69 13
AflII C'TTAA_G
Cuts at: 0 1366 1578
Size: 1366 212
AhdI GACnn_n'nnGTC
Cuts at: 0 1134 1578
Size: 1134 444
AlwNI CAG_nnn'CTG
Cuts at: 0 26 1578
Size: 26 1552
ApaLI G'TGCA_C
Cuts at: 0 1066 1578
Size: 1066 512
AvaI C'yCGr_G
Cuts at: 0 1102 1187 1578
Size: 1102 85 391
Fragments arranged by size:
1102 391 85
AvrII C'CTAG_G
Cuts at: 0 594 1578
Size: 594 984
BamHI G'GATC_C
Cuts at: 0 899 1578
Size: 899 679
BanI G'GyrC_C
Cuts at: 0 1206 1578
Size: 1206 372
BanII G_rGCy'C
Cuts at: 0 75 341 461 717 776 1109 1154 1578
Size: 75 266 120 256 59 333 45 424
Fragments arranged by size:
424 333 266 256 120 75 59 45
BbsI GAAGACnn'nnnn_
Cuts at: 0 987 1099 1226 1541 1578
Size: 987 112 127 315 37
Fragments arranged by size:
987 315 127 112 37
BbvCI CC'TCA_GC
Cuts at: 0 908 1361 1578
Size: 908 453 217
Fragments arranged by size:
908 453 217
BglII A'GATC_T
Cuts at: 0 704 1578
Size: 704 874
BlpI GC'TnA_GC
Cuts at: 0 572 674 1174 1578
Size: 572 102 500 404
Fragments arranged by size:
572 500 404 102
Bme1580I G_kGCm'C
Cuts at: 0 1070 1211 1578
Size: 1070 141 367
Fragments arranged by size:
1070 367 141
BmrI ACTGGGnnnn_n'
Cuts at: 0 837 1578
Size: 837 741
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 253 948 1578
Size: 253 695 630
Fragments arranged by size:
695 630 253
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 325 760 1168 1447 1578
Size: 325 435 408 279 131
Fragments arranged by size:
435 408 325 279 131
Bpu10I CC'TnA_GC
Cuts at: 0 908 1361 1578
Size: 908 453 217
Fragments arranged by size:
908 453 217
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1045 1578
Size: 1045 533
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 91 121 1136 1166 1578
Size: 91 30 1015 30 412
Fragments arranged by size:
1015 412 91 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 947 1578
Size: 947 631
BseYI C'CCAG_C
Cuts at: 0 951 986 1578
Size: 951 35 592
Fragments arranged by size:
951 592 35
BsiEI CG_ry'CG
Cuts at: 0 611 1578
Size: 611 967
BsiHKAI G_wGCw'C
Cuts at: 0 776 1070 1154 1578
Size: 776 294 84 424
Fragments arranged by size:
776 424 294 84
Bsp1286I G_dGCh'C
Cuts at: 0 75 341 461 717 776 1070 1109 1154
Size: 75 266 120 256 59 294 39 45
Cuts at: 1154 1211 1578
Size: 57 367
Fragments arranged by size:
367 294 266 256 120 75 59 57
45 39
BspHI T'CATG_A
Cuts at: 0 1088 1578
Size: 1088 490
BsrDI GCAATG_nn'
Cuts at: 0 377 825 1203 1578
Size: 377 448 378 375
Fragments arranged by size:
448 378 377 375
BsrGI T'GTAC_A
Cuts at: 0 137 1578
Size: 137 1441
BssSI C'ACGA_G
Cuts at: 0 441 1578
Size: 441 1137
BstEII G'GTnAC_C
Cuts at: 0 1291 1578
Size: 1291 287
BstYI r'GATC_y
Cuts at: 0 329 704 899 1578
Size: 329 375 195 679
Fragments arranged by size:
679 375 329 195
Bsu36I CC'TnA_GG
Cuts at: 0 1022 1578
Size: 1022 556
BtgI C'CryG_G
Cuts at: 0 833 1578
Size: 833 745
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 499 1578
Size: 499 1079
BtsI GCAGTG_nn'
Cuts at: 0 218 1578
Size: 218 1360
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 593 628 1578
Size: 593 35 950
Fragments arranged by size:
950 593 35
DraIII CAC_nnn'GTG
Cuts at: 0 738 1578
Size: 738 840
EaeI y'GGCC_r
Cuts at: 0 437 578 1578
Size: 437 141 1000
Fragments arranged by size:
1000 437 141
EarI CTCTTCn'nnn_
Cuts at: 0 764 1010 1399 1578
Size: 764 246 389 179
Fragments arranged by size:
764 389 246 179
EcoICRI GAG'CTC
Cuts at: 0 774 1152 1578
Size: 774 378 426
Fragments arranged by size:
774 426 378
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 69 169 182 325 440 693 760 1168
Size: 69 100 13 143 115 253 67 408
Cuts at: 1168 1380 1447 1578
Size: 212 67 131
Fragments arranged by size:
408 253 212 143 131 115 100 69
67 67 13
EcoNI CCTnn'n_nnAGG
Cuts at: 0 10 598 1578
Size: 10 588 980
Fragments arranged by size:
980 588 10
EcoO109I rG'GnC_Cy
Cuts at: 0 99 1422 1578
Size: 99 1323 156
Fragments arranged by size:
1323 156 99
FspI TGC'GCA
Cuts at: 0 1542 1578
Size: 1542 36
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 91 123 625 657 891 923 1120 1152
Size: 91 32 502 32 234 32 197 32
Cuts at: 1152 1578
Size: 426
Fragments arranged by size:
502 426 234 197 91 32 32 32
32
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 249 968 1578
Size: 249 719 610
Fragments arranged by size:
719 610 249
MscI TGG'CCA
Cuts at: 0 439 580 1578
Size: 439 141 998
Fragments arranged by size:
998 439 141
MslI CAynn'nnrTG
Cuts at: 0 445 838 1578
Size: 445 393 740
Fragments arranged by size:
740 445 393
MspA1I CmG'CkG
Cuts at: 0 49 315 576 673 990 1578
Size: 49 266 261 97 317 588
Fragments arranged by size:
588 317 266 261 97 49
NspI r_CATG'y
Cuts at: 0 138 1578
Size: 138 1440
PfoI T'CCnGG_A
Cuts at: 0 95 109 1124 1578
Size: 95 14 1015 454
Fragments arranged by size:
1015 454 95 14
PpuMI rG'GwC_Cy
Cuts at: 0 99 1422 1578
Size: 99 1323 156
Fragments arranged by size:
1323 156 99
PvuII CAG'CTG
Cuts at: 0 315 576 673 990 1578
Size: 315 261 97 317 588
Fragments arranged by size:
588 317 315 261 97
SacI G_AGCT'C
Cuts at: 0 776 1154 1578
Size: 776 378 424
Fragments arranged by size:
776 424 378
SapI GCTCTTCn'nnn_
Cuts at: 0 764 1010 1578
Size: 764 246 568
Fragments arranged by size:
764 568 246
SfcI C'TryA_G
Cuts at: 0 1113 1547 1578
Size: 1113 434 31
Fragments arranged by size:
1113 434 31
SmaI CCC'GGG
Cuts at: 0 1104 1578
Size: 1104 474
SmlI C'TyrA_G
Cuts at: 0 1060 1366 1578
Size: 1060 306 212
Fragments arranged by size:
1060 306 212
SrfI GCCC'GGGC
Cuts at: 0 1104 1578
Size: 1104 474
StuI AGG'CCT
Cuts at: 0 175 1578
Size: 175 1403
StyI C'CwwG_G
Cuts at: 0 594 1296 1316 1578
Size: 594 702 20 262
Fragments arranged by size:
702 594 262 20
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 699 705 1279 1578
Size: 699 6 574 299
Fragments arranged by size:
699 574 299 6
TatI w'GTAC_w
Cuts at: 0 137 1076 1578
Size: 137 939 502
Fragments arranged by size:
939 502 137
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1477 1509 1578
Size: 1477 32 69
Fragments arranged by size:
1477 69 32
XmaI C'CCGG_G
Cuts at: 0 1102 1578
Size: 1102 476
Enzymes that do cut:
AcuI AflII AhdI AlwNI ApaLI AvaI AvrII BamHI
BanI BanII BbsI BbvCI BglII BlpI Bme1580I BmrI
BplI BpmI Bpu10I BpuEI BsaXI BseRI BseYI BsiEI
BsiHKAI Bsp1286I BspHI BsrDI BsrGI BssSI BstEII BstYI
Bsu36I BtgI BtgZI BtsI CspCI DraIII EaeI EarI
EcoICRI Eco57MI EcoNI EcoO109I FspI Hin4I MmeI MscI
MslI MspA1I NspI PfoI PpuMI PvuII SacI SapI
SfcI SmaI SmlI SrfI StuI StyI TaqII TatI
TstI XmaI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflIII AgeI
AleI AlfI AloI ApaI ApoI AscI AseI AsiSI
BaeI BbeI BcgI BciVI BclI BfrBI BglI BmgBI
BmtI BsaI BsaAI BsaBI BsaHI BsaWI BsgI BsiWI
BsmI BsmBI BspEI BspMI BsrBI BsrFI BssHII BstAPI
BstBI BstXI BstZ17I ClaI DraI DrdI EagI EciI
EcoRI EcoRV FalI FseI FspAI HaeII HincII HindIII
HpaI KasI KpnI MfeI MluI NaeI NarI NcoI
NdeI NgoMIV NheI NotI NruI NsiI PacI PasI
PciI PflMI PmeI PmlI PpiI PshAI PsiI PspOMI
PspXI PsrI PstI PvuI RsrII SacII SalI SanDI
SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SnaBI
SpeI SphI SspI SwaI Tth111I XbaI XcmI XhoI
XmnI ZraI