Restriction map for
pFN21AE1376
(Linear) (Six-base) MAPSORT of: KIEE1376.seq Check: 9940 from: 1 to: 582
KIEE1376.orf
With 182 enzymes: SgfI *
July 15, 2010 18:37 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 34 582
Size: 34 548
AlwNI CAG_nnn'CTG
Cuts at: 0 364 582
Size: 364 218
BanI G'GyrC_C
Cuts at: 0 64 582
Size: 64 518
BanII G_rGCy'C
Cuts at: 0 532 582
Size: 532 50
BbsI GAAGACnn'nnnn_
Cuts at: 0 537 582
Size: 537 45
BbvCI CC'TCA_GC
Cuts at: 0 524 582
Size: 524 58
BglI GCCn_nnn'nGGC
Cuts at: 0 241 582
Size: 241 341
Bme1580I G_kGCm'C
Cuts at: 0 67 582
Size: 67 515
BmrI ACTGGGnnnn_n'
Cuts at: 0 63 582
Size: 63 519
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 159 582
Size: 159 423
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 226 469 582
Size: 226 243 113
Fragments arranged by size:
243 226 113
Bpu10I CC'TnA_GC
Cuts at: 0 54 144 524 582
Size: 54 90 380 58
Fragments arranged by size:
380 90 58 54
BsaI GGTCTCn'nnnn_
Cuts at: 0 361 394 424 445 582
Size: 361 33 30 21 137
Fragments arranged by size:
361 137 33 30 21
BsaHI Gr'CG_yC
Cuts at: 0 572 582
Size: 572 10
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 199 229 582
Size: 199 30 353
Fragments arranged by size:
353 199 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 442 582
Size: 442 140
BseYI C'CCAG_C
Cuts at: 0 377 582
Size: 377 205
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 106 316 543 582
Size: 106 210 227 39
Fragments arranged by size:
227 210 106 39
Bsp1286I G_dGCh'C
Cuts at: 0 67 532 582
Size: 67 465 50
Fragments arranged by size:
465 67 50
BtgI C'CryG_G
Cuts at: 0 236 286 533 582
Size: 236 50 247 49
Fragments arranged by size:
247 236 50 49
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 465 582
Size: 465 117
BtsI GCAGTG_nn'
Cuts at: 0 337 582
Size: 337 245
DraIII CAC_nnn'GTG
Cuts at: 0 71 582
Size: 71 511
EaeI y'GGCC_r
Cuts at: 0 152 242 582
Size: 152 90 340
Fragments arranged by size:
340 152 90
EarI CTCTTCn'nnn_
Cuts at: 0 302 582
Size: 302 280
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 34 226 469 582
Size: 34 192 243 113
Fragments arranged by size:
243 192 113 34
EcoNI CCTnn'n_nnAGG
Cuts at: 0 53 582
Size: 53 529
EcoO109I rG'GnC_Cy
Cuts at: 0 120 361 582
Size: 120 241 221
Fragments arranged by size:
241 221 120
FspI TGC'GCA
Cuts at: 0 511 582
Size: 511 71
HincII GTy'rAC
Cuts at: 0 519 582
Size: 519 63
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 152 197 582
Size: 152 45 385
Fragments arranged by size:
385 152 45
MscI TGG'CCA
Cuts at: 0 244 582
Size: 244 338
MslI CAynn'nnrTG
Cuts at: 0 34 582
Size: 34 548
MspA1I CmG'CkG
Cuts at: 0 195 288 381 407 582
Size: 195 93 93 26 175
Fragments arranged by size:
195 175 93 93 26
NcoI C'CATG_G
Cuts at: 0 236 582
Size: 236 346
NspI r_CATG'y
Cuts at: 0 225 582
Size: 225 357
PfoI T'CCnGG_A
Cuts at: 0 227 582
Size: 227 355
PpuMI rG'GwC_Cy
Cuts at: 0 120 582
Size: 120 462
PvuII CAG'CTG
Cuts at: 0 195 582
Size: 195 387
RsrII CG'GwC_CG
Cuts at: 0 131 582
Size: 131 451
SacII CC_GC'GG
Cuts at: 0 289 582
Size: 289 293
StyI C'CwwG_G
Cuts at: 0 236 582
Size: 236 346
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 31 82 118 577 582
Size: 31 51 36 459 5
Fragments arranged by size:
459 51 36 31 5
Tth111I GACn'n_nGTC
Cuts at: 0 541 582
Size: 541 41
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 411 582
Size: 411 171
Enzymes that do cut:
AcuI AlwNI BanI BanII BbsI BbvCI BglI Bme1580I
BmrI BplI BpmI Bpu10I BsaI BsaHI BsaXI BseRI
BseYI BsgI Bsp1286I BtgI BtgZI BtsI DraIII EaeI
EarI Eco57MI EcoNI EcoO109I FspI HincII MmeI MscI
MslI MspA1I NcoI NspI PfoI PpuMI PvuII RsrII
SacII StyI TaqII Tth111I XcmI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI ApaI ApaLI ApoI
AscI AseI AsiSI AvaI AvrII BaeI BamHI BbeI
BcgI BciVI BclI BfrBI BglII BlpI BmgBI BmtI
BpuEI BsaAI BsaBI BsaWI BsiEI BsiHKAI BsiWI BsmI
BsmBI BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I
Bsu36I ClaI CspCI DraI DrdI EagI EciI EcoICRI
EcoRI EcoRV FalI FseI FspAI HaeII Hin4I HindIII
HpaI KasI KpnI MfeI MluI NaeI NarI NdeI
NgoMIV NheI NotI NruI NsiI PacI PasI PciI
PflMI PmeI PmlI PpiI PshAI PsiI PspOMI PspXI
PsrI PstI PvuI SacI SalI SanDI SapI SbfI
ScaI SexAI SfcI SfiI SfoI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SrfI SspI StuI SwaI
TatI TstI XbaI XhoI XmaI XmnI ZraI