Restriction map for
pFN21AE1373
(Linear) (Six-base) MAPSORT of: KIEE1373.seq Check: 7622 from: 1 to: 1317
KIEE1373.orf
With 182 enzymes: SgfI *
July 15, 2010 18:37 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 563 1317
Size: 563 754
AleI CACnn'nnGTG
Cuts at: 0 1232 1317
Size: 1232 85
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 496 528 1317
Size: 496 32 789
Fragments arranged by size:
789 496 32
AlwNI CAG_nnn'CTG
Cuts at: 0 109 1317
Size: 109 1208
ApaI G_GGCC'C
Cuts at: 0 74 556 856 1000 1317
Size: 74 482 300 144 317
Fragments arranged by size:
482 317 300 144 74
ApoI r'AATT_y
Cuts at: 0 1141 1317
Size: 1141 176
AvaI C'yCGr_G
Cuts at: 0 31 533 1317
Size: 31 502 784
Fragments arranged by size:
784 502 31
BamHI G'GATC_C
Cuts at: 0 215 1317
Size: 215 1102
BanII G_rGCy'C
Cuts at: 0 74 556 565 650 830 856 1000 1250
Size: 74 482 9 85 180 26 144 250
Cuts at: 1250 1317
Size: 67
Fragments arranged by size:
482 250 180 144 85 74 67 26
9
BbvCI CC'TCA_GC
Cuts at: 0 1276 1317
Size: 1276 41
BclI T'GATC_A
Cuts at: 0 113 950 1317
Size: 113 837 367
Fragments arranged by size:
837 367 113
BlpI GC'TnA_GC
Cuts at: 0 37 98 1317
Size: 37 61 1219
Fragments arranged by size:
1219 61 37
Bme1580I G_kGCm'C
Cuts at: 0 74 556 856 1000 1317
Size: 74 482 300 144 317
Fragments arranged by size:
482 317 300 144 74
BmgBI CAC'GTC
Cuts at: 0 787 1317
Size: 787 530
BmrI ACTGGGnnnn_n'
Cuts at: 0 626 1226 1317
Size: 626 600 91
Fragments arranged by size:
626 600 91
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 123 1317
Size: 123 1194
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 178 361 781 1264 1317
Size: 178 183 420 483 53
Fragments arranged by size:
483 420 183 178 53
Bpu10I CC'TnA_GC
Cuts at: 0 1276 1317
Size: 1276 41
BsaI GGTCTCn'nnnn_
Cuts at: 0 629 1260 1264 1317
Size: 629 631 4 53
Fragments arranged by size:
631 629 53 4
BsaAI yAC'GTr
Cuts at: 0 60 1317
Size: 60 1257
BsaWI w'CCGG_w
Cuts at: 0 892 1317
Size: 892 425
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 404 434 874 892 895 904 922 925
Size: 404 30 440 18 3 9 18 3
Cuts at: 925 1007 1037 1317
Size: 82 30 280
Fragments arranged by size:
440 404 280 82 30 30 18 18
9 3 3
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 900 1317
Size: 900 417
BseYI C'CCAG_C
Cuts at: 0 391 823 928 1317
Size: 391 432 105 389
Fragments arranged by size:
432 391 389 105
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 82 709 1317
Size: 82 627 608
Fragments arranged by size:
627 608 82
BsiEI CG_ry'CG
Cuts at: 0 994 1317
Size: 994 323
BsiHKAI G_wGCw'C
Cuts at: 0 1250 1317
Size: 1250 67
BsmBI CGTCTCn'nnnn_
Cuts at: 0 154 1317
Size: 154 1163
Bsp1286I G_dGCh'C
Cuts at: 0 74 556 565 650 830 856 1000 1250
Size: 74 482 9 85 180 26 144 250
Cuts at: 1250 1317
Size: 67
Fragments arranged by size:
482 250 180 144 85 74 67 26
9
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 563 1226 1317
Size: 563 663 91
Fragments arranged by size:
663 563 91
BsrBI CCG'CTC
Cuts at: 0 866 1317
Size: 866 451
BsrFI r'CCGG_y
Cuts at: 0 748 809 1317
Size: 748 61 508
Fragments arranged by size:
748 508 61
BsrGI T'GTAC_A
Cuts at: 0 770 1317
Size: 770 547
BssHII G'CGCG_C
Cuts at: 0 606 1317
Size: 606 711
BssSI C'ACGA_G
Cuts at: 0 1249 1317
Size: 1249 68
BstEII G'GTnAC_C
Cuts at: 0 402 1317
Size: 402 915
BstXI CCAn_nnnn'nTGG
Cuts at: 0 399 523 805 1317
Size: 399 124 282 512
Fragments arranged by size:
512 399 282 124
BstYI r'GATC_y
Cuts at: 0 215 962 1317
Size: 215 747 355
Fragments arranged by size:
747 355 215
Bsu36I CC'TnA_GG
Cuts at: 0 139 408 1011 1317
Size: 139 269 603 306
Fragments arranged by size:
603 306 269 139
BtgI C'CryG_G
Cuts at: 0 522 548 1317
Size: 522 26 769
Fragments arranged by size:
769 522 26
BtsI GCAGTG_nn'
Cuts at: 0 336 1317
Size: 336 981
EaeI y'GGCC_r
Cuts at: 0 807 991 1317
Size: 807 184 326
Fragments arranged by size:
807 326 184
EagI C'GGCC_G
Cuts at: 0 991 1317
Size: 991 326
EcoICRI GAG'CTC
Cuts at: 0 1248 1317
Size: 1248 69
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 178 361 781 1264 1317
Size: 178 183 420 483 53
Fragments arranged by size:
483 420 183 178 53
EcoO109I rG'GnC_Cy
Cuts at: 0 53 70 71 553 697 853 997 1178
Size: 53 17 1 482 144 156 144 181
Cuts at: 1178 1317
Size: 139
Fragments arranged by size:
482 181 156 144 144 139 53 17
1
EcoRI G'AATT_C
Cuts at: 0 1141 1317
Size: 1141 176
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 744 1317
Size: 744 573
HaeII r_GCGC'y
Cuts at: 0 804 1119 1317
Size: 804 315 198
Fragments arranged by size:
804 315 198
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 552 584 965 997 1317
Size: 552 32 381 32 320
Fragments arranged by size:
552 381 320 32 32
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1232 1317
Size: 1232 85
MslI CAynn'nnrTG
Cuts at: 0 515 521 1232 1317
Size: 515 6 711 85
Fragments arranged by size:
711 515 85 6
MspA1I CmG'CkG
Cuts at: 0 1087 1098 1267 1317
Size: 1087 11 169 50
Fragments arranged by size:
1087 169 50 11
NaeI GCC'GGC
Cuts at: 0 750 811 1317
Size: 750 61 506
Fragments arranged by size:
750 506 61
NgoMIV G'CCGG_C
Cuts at: 0 748 809 1317
Size: 748 61 508
Fragments arranged by size:
748 508 61
NspI r_CATG'y
Cuts at: 0 277 890 1317
Size: 277 613 427
Fragments arranged by size:
613 427 277
PasI CC'CwG_GG
Cuts at: 0 712 849 1317
Size: 712 137 468
Fragments arranged by size:
712 468 137
PflMI CCAn_nnn'nTGG
Cuts at: 0 92 266 1317
Size: 92 174 1051
Fragments arranged by size:
1051 174 92
PmlI CAC'GTG
Cuts at: 0 60 1317
Size: 60 1257
PpuMI rG'GwC_Cy
Cuts at: 0 53 697 1178 1317
Size: 53 644 481 139
Fragments arranged by size:
644 481 139 53
PspOMI G'GGCC_C
Cuts at: 0 70 552 852 996 1317
Size: 70 482 300 144 321
Fragments arranged by size:
482 321 300 144 70
PvuII CAG'CTG
Cuts at: 0 1087 1267 1317
Size: 1087 180 50
Fragments arranged by size:
1087 180 50
SacI G_AGCT'C
Cuts at: 0 1250 1317
Size: 1250 67
SfcI C'TryA_G
Cuts at: 0 311 363 1182 1317
Size: 311 52 819 135
Fragments arranged by size:
819 311 135 52
SmaI CCC'GGG
Cuts at: 0 535 1317
Size: 535 782
StuI AGG'CCT
Cuts at: 0 317 1317
Size: 317 1000
StyI C'CwwG_G
Cuts at: 0 120 1073 1304 1317
Size: 120 953 231 13
Fragments arranged by size:
953 231 120 13
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 133 506 1317
Size: 133 373 811
Fragments arranged by size:
811 373 133
TatI w'GTAC_w
Cuts at: 0 770 881 1317
Size: 770 111 436
Fragments arranged by size:
770 436 111
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 404 436 1007 1039 1317
Size: 404 32 571 32 278
Fragments arranged by size:
571 404 278 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 546 572 639 846 936 1239 1317
Size: 546 26 67 207 90 303 78
Fragments arranged by size:
546 303 207 90 78 67 26
XmaI C'CCGG_G
Cuts at: 0 533 1317
Size: 533 784
Enzymes that do cut:
AarI AleI AloI AlwNI ApaI ApoI AvaI BamHI
BanII BbvCI BclI BlpI Bme1580I BmgBI BmrI BplI
BpmI Bpu10I BsaI BsaAI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspMI BsrBI BsrFI
BsrGI BssHII BssSI BstEII BstXI BstYI Bsu36I BtgI
BtsI EaeI EagI EcoICRI Eco57MI EcoO109I EcoRI FalI
HaeII Hin4I MmeI MslI MspA1I NaeI NgoMIV NspI
PasI PflMI PmlI PpuMI PspOMI PvuII SacI SfcI
SmaI StuI StyI TaqII TatI TstI XcmI XmaI
Enzymes that do not cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AlfI ApaLI AscI AseI AsiSI AvrII
BaeI BanI BbeI BbsI BcgI BciVI BfrBI BglI
BglII BmtI BpuEI BsaBI BsaHI BsiWI BsmI BspEI
BspHI BsrDI BstAPI BstBI BstZ17I BtgZI ClaI CspCI
DraI DraIII DrdI EarI EciI EcoNI EcoRV FseI
FspI FspAI HincII HindIII HpaI KasI KpnI MfeI
MluI MscI NarI NcoI NdeI NheI NotI NruI
NsiI PacI PciI PfoI PmeI PpiI PshAI PsiI
PspXI PsrI PstI PvuI RsrII SacII SalI SanDI
SapI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI
SmlI SnaBI SpeI SphI SrfI SspI SwaI Tth111I
XbaI XhoI XmnI ZraI