Restriction map for
pFN21AE1370
(Linear) (Six-base) MAPSORT of: KIEE1370.seq Check: 2834 from: 1 to: 747
KIEE1370.orf
With 182 enzymes: SgfI *
July 15, 2010 18:37 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 598 747
Size: 598 149
AleI CACnn'nnGTG
Cuts at: 0 115 271 747
Size: 115 156 476
Fragments arranged by size:
476 156 115
AlwNI CAG_nnn'CTG
Cuts at: 0 63 747
Size: 63 684
ApoI r'AATT_y
Cuts at: 0 718 747
Size: 718 29
BanI G'GyrC_C
Cuts at: 0 3 99 747
Size: 3 96 648
Fragments arranged by size:
648 96 3
BanII G_rGCy'C
Cuts at: 0 74 747
Size: 74 673
BbeI G_GCGC'C
Cuts at: 0 7 747
Size: 7 740
BclI T'GATC_A
Cuts at: 0 248 747
Size: 248 499
Bme1580I G_kGCm'C
Cuts at: 0 287 747
Size: 287 460
BmrI ACTGGGnnnn_n'
Cuts at: 0 236 650 747
Size: 236 414 97
Fragments arranged by size:
414 236 97
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 492 747
Size: 492 255
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 694 747
Size: 694 53
BsaAI yAC'GTr
Cuts at: 0 269 747
Size: 269 478
BsaHI Gr'CG_yC
Cuts at: 0 4 747
Size: 4 743
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 620 650 747
Size: 620 30 97
Fragments arranged by size:
620 97 30
BsiEI CG_ry'CG
Cuts at: 0 107 747
Size: 107 640
BsiHKAI G_wGCw'C
Cuts at: 0 74 129 613 747
Size: 74 55 484 134
Fragments arranged by size:
484 134 74 55
BsmBI CGTCTCn'nnnn_
Cuts at: 0 594 747
Size: 594 153
Bsp1286I G_dGCh'C
Cuts at: 0 74 129 287 613 747
Size: 74 55 158 326 134
Fragments arranged by size:
326 158 134 74 55
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 658 747
Size: 658 89
BsrFI r'CCGG_y
Cuts at: 0 102 747
Size: 102 645
BssSI C'ACGA_G
Cuts at: 0 556 747
Size: 556 191
BstXI CCAn_nnnn'nTGG
Cuts at: 0 273 747
Size: 273 474
BstYI r'GATC_y
Cuts at: 0 168 747
Size: 168 579
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 435 747
Size: 435 312
EaeI y'GGCC_r
Cuts at: 0 92 104 662 747
Size: 92 12 558 85
Fragments arranged by size:
558 92 85 12
EagI C'GGCC_G
Cuts at: 0 104 747
Size: 104 643
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 66 747
Size: 66 681
EcoICRI GAG'CTC
Cuts at: 0 72 747
Size: 72 675
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 492 598 747
Size: 492 106 149
Fragments arranged by size:
492 149 106
EcoRI G'AATT_C
Cuts at: 0 718 747
Size: 718 29
FspI TGC'GCA
Cuts at: 0 191 747
Size: 191 556
HaeII r_GCGC'y
Cuts at: 0 7 747
Size: 7 740
KasI G'GCGC_C
Cuts at: 0 3 747
Size: 3 744
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 608 747
Size: 608 139
MscI TGG'CCA
Cuts at: 0 664 747
Size: 664 83
MslI CAynn'nnrTG
Cuts at: 0 115 271 747
Size: 115 156 476
Fragments arranged by size:
476 156 115
NaeI GCC'GGC
Cuts at: 0 104 747
Size: 104 643
NarI GG'CG_CC
Cuts at: 0 4 747
Size: 4 743
NgoMIV G'CCGG_C
Cuts at: 0 102 747
Size: 102 645
NspI r_CATG'y
Cuts at: 0 304 747
Size: 304 443
PflMI CCAn_nnn'nTGG
Cuts at: 0 351 747
Size: 351 396
PfoI T'CCnGG_A
Cuts at: 0 10 747
Size: 10 737
PmlI CAC'GTG
Cuts at: 0 269 747
Size: 269 478
PshAI GACnn'nnGTC
Cuts at: 0 445 747
Size: 445 302
SacI G_AGCT'C
Cuts at: 0 74 747
Size: 74 673
SfoI GGC'GCC
Cuts at: 0 5 747
Size: 5 742
SmlI C'TyrA_G
Cuts at: 0 709 747
Size: 709 38
StyI C'CwwG_G
Cuts at: 0 95 747
Size: 95 652
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 261 685 747
Size: 261 424 62
Fragments arranged by size:
424 261 62
TatI w'GTAC_w
Cuts at: 0 552 747
Size: 552 195
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 620 652 747
Size: 620 32 95
Fragments arranged by size:
620 95 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 180 747
Size: 180 567
XmnI GAAnn'nnTTC
Cuts at: 0 19 747
Size: 19 728
Enzymes that do cut:
AcuI AleI AlwNI ApoI BanI BanII BbeI BclI
Bme1580I BmrI BpmI BpuEI BsaAI BsaHI BsaXI BsiEI
BsiHKAI BsmBI Bsp1286I BspMI BsrFI BssSI BstXI BstYI
BtgZI EaeI EagI EciI EcoICRI Eco57MI EcoRI FspI
HaeII KasI MmeI MscI MslI NaeI NarI NgoMIV
NspI PflMI PfoI PmlI PshAI SacI SfoI SmlI
StyI TaqII TatI TstI XcmI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AgeI AhdI AlfI AloI ApaI ApaLI AscI AseI
AsiSI AvaI AvrII BaeI BamHI BbsI BbvCI BcgI
BciVI BfrBI BglI BglII BlpI BmgBI BmtI BplI
Bpu10I BsaI BsaBI BsaWI BseRI BseYI BsgI BsiWI
BsmI BspEI BspHI BsrBI BsrDI BsrGI BssHII BstAPI
BstBI BstEII BstZ17I Bsu36I BtgI BtsI ClaI CspCI
DraI DraIII DrdI EarI EcoNI EcoO109I EcoRV FalI
FseI FspAI Hin4I HincII HindIII HpaI KpnI MfeI
MluI MspA1I NcoI NdeI NheI NotI NruI NsiI
PacI PasI PciI PmeI PpiI PpuMI PsiI PspOMI
PspXI PsrI PstI PvuI PvuII RsrII SacII SalI
SanDI SapI SbfI ScaI SexAI SfcI SfiI SgfI
SgrAI SmaI SnaBI SpeI SphI SrfI SspI StuI
SwaI Tth111I XbaI XhoI XmaI ZraI