Restriction map for
pFN21AE1369
(Linear) (Six-base) MAPSORT of: KIEE1369.seq Check: 8639 from: 1 to: 1614
KIEE1369.orf
With 182 enzymes: SgfI *
July 15, 2010 18:37 ..
AccI GT'mk_AC
Cuts at: 0 1346 1614
Size: 1346 268
Acc65I G'GTAC_C
Cuts at: 0 824 1614
Size: 824 790
AgeI A'CCGG_T
Cuts at: 0 868 1614
Size: 868 746
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 442 1064 1614
Size: 442 622 550
Fragments arranged by size:
622 550 442
AlwNI CAG_nnn'CTG
Cuts at: 0 1062 1614
Size: 1062 552
ApaI G_GGCC'C
Cuts at: 0 146 752 969 1614
Size: 146 606 217 645
Fragments arranged by size:
645 606 217 146
AvaI C'yCGr_G
Cuts at: 0 91 146 191 287 413 903 962 1614
Size: 91 55 45 96 126 490 59 652
Fragments arranged by size:
652 490 126 96 91 59 55 45
AvrII C'CTAG_G
Cuts at: 0 130 1614
Size: 130 1484
BanI G'GyrC_C
Cuts at: 0 107 243 817 824 865 1006 1614
Size: 107 136 574 7 41 141 608
Fragments arranged by size:
608 574 141 136 107 41 7
BanII G_rGCy'C
Cuts at: 0 146 338 526 620 720 752 891 969
Size: 146 192 188 94 100 32 139 78
Cuts at: 969 1614
Size: 645
Fragments arranged by size:
645 192 188 146 139 100 94 78
32
BbeI G_GCGC'C
Cuts at: 0 111 247 821 1614
Size: 111 136 574 793
Fragments arranged by size:
793 574 136 111
BbvCI CC'TCA_GC
Cuts at: 0 352 1614
Size: 352 1262
BciVI GTATCCnnnnn_n'
Cuts at: 0 701 1614
Size: 701 913
BfrBI ATG'CAT
Cuts at: 0 1286 1614
Size: 1286 328
BglI GCCn_nnn'nGGC
Cuts at: 0 416 537 551 964 1057 1310 1614
Size: 416 121 14 413 93 253 304
Fragments arranged by size:
416 413 304 253 121 93 14
BlpI GC'TnA_GC
Cuts at: 0 215 647 1614
Size: 215 432 967
Fragments arranged by size:
967 432 215
Bme1580I G_kGCm'C
Cuts at: 0 146 752 868 969 1550 1614
Size: 146 606 116 101 581 64
Fragments arranged by size:
606 581 146 116 101 64
BmrI ACTGGGnnnn_n'
Cuts at: 0 167 1077 1614
Size: 167 910 537
Fragments arranged by size:
910 537 167
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 55 352 476 734 946 1614
Size: 55 297 124 258 212 668
Fragments arranged by size:
668 297 258 212 124 55
Bpu10I CC'TnA_GC
Cuts at: 0 352 1614
Size: 352 1262
BsaHI Gr'CG_yC
Cuts at: 0 108 244 818 1614
Size: 108 136 574 796
Fragments arranged by size:
796 574 136 108
BsaWI w'CCGG_w
Cuts at: 0 868 951 1614
Size: 868 83 663
Fragments arranged by size:
868 663 83
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1394 1424 1614
Size: 1394 30 190
Fragments arranged by size:
1394 190 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 164 943 1614
Size: 164 779 671
Fragments arranged by size:
779 671 164
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1409 1614
Size: 1409 205
BsiEI CG_ry'CG
Cuts at: 0 48 1614
Size: 48 1566
BsiHKAI G_wGCw'C
Cuts at: 0 573 620 720 1614
Size: 573 47 100 894
Fragments arranged by size:
894 573 100 47
BsmBI CGTCTCn'nnnn_
Cuts at: 0 753 1614
Size: 753 861
Bsp1286I G_dGCh'C
Cuts at: 0 146 338 526 573 620 720 752 868
Size: 146 192 188 47 47 100 32 116
Cuts at: 868 891 969 1550 1614
Size: 23 78 581 64
Fragments arranged by size:
581 192 188 146 116 100 78 64
47 47 32 23
BspEI T'CCGG_A
Cuts at: 0 951 1614
Size: 951 663
BsrBI CCG'CTC
Cuts at: 0 6 362 742 1614
Size: 6 356 380 872
Fragments arranged by size:
872 380 356 6
BsrDI GCAATG_nn'
Cuts at: 0 1053 1256 1614
Size: 1053 203 358
Fragments arranged by size:
1053 358 203
BsrFI r'CCGG_y
Cuts at: 0 68 186 464 573 793 868 1614
Size: 68 118 278 109 220 75 746
Fragments arranged by size:
746 278 220 118 109 75 68
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1052 1292 1301 1614
Size: 1052 240 9 313
Fragments arranged by size:
1052 313 240 9
BstYI r'GATC_y
Cuts at: 0 1238 1614
Size: 1238 376
BstZ17I GTA'TAC
Cuts at: 0 1347 1614
Size: 1347 267
BtgI C'CryG_G
Cuts at: 0 269 338 516 656 812 891 1451 1560
Size: 269 69 178 140 156 79 560 109
Cuts at: 1560 1614
Size: 54
Fragments arranged by size:
560 269 178 156 140 109 79 69
54
DraIII CAC_nnn'GTG
Cuts at: 0 789 1085 1614
Size: 789 296 529
Fragments arranged by size:
789 529 296
EaeI y'GGCC_r
Cuts at: 0 45 383 563 1398 1614
Size: 45 338 180 835 216
Fragments arranged by size:
835 338 216 180 45
EagI C'GGCC_G
Cuts at: 0 45 1614
Size: 45 1569
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 464 594 1614
Size: 464 130 1020
Fragments arranged by size:
1020 464 130
EcoICRI GAG'CTC
Cuts at: 0 618 718 1614
Size: 618 100 896
Fragments arranged by size:
896 618 100
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 55 352 476 734 946 1614
Size: 55 297 124 258 212 668
Fragments arranged by size:
668 297 258 212 124 55
EcoO109I rG'GnC_Cy
Cuts at: 0 86 127 142 143 163 597 748 749
Size: 86 41 15 1 20 434 151 1
Cuts at: 749 940 1059 1614
Size: 191 119 555
Fragments arranged by size:
555 434 191 151 119 86 41 20
15 1 1
EcoRV GAT'ATC
Cuts at: 0 1448 1614
Size: 1448 166
HaeII r_GCGC'y
Cuts at: 0 111 247 516 821 1614
Size: 111 136 269 305 793
Fragments arranged by size:
793 305 269 136 111
KasI G'GCGC_C
Cuts at: 0 107 243 817 1614
Size: 107 136 574 797
Fragments arranged by size:
797 574 136 107
KpnI G_GTAC'C
Cuts at: 0 828 1614
Size: 828 786
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 161 1361 1614
Size: 161 1200 253
Fragments arranged by size:
1200 253 161
MscI TGG'CCA
Cuts at: 0 1400 1614
Size: 1400 214
MslI CAynn'nnrTG
Cuts at: 0 496 1614
Size: 496 1118
MspA1I CmG'CkG
Cuts at: 0 271 488 535 651 658 893 1198 1506
Size: 271 217 47 116 7 235 305 308
Cuts at: 1506 1578 1614
Size: 72 36
Fragments arranged by size:
308 305 271 235 217 116 72 47
36 7
NaeI GCC'GGC
Cuts at: 0 188 466 1614
Size: 188 278 1148
Fragments arranged by size:
1148 278 188
NarI GG'CG_CC
Cuts at: 0 108 244 818 1614
Size: 108 136 574 796
Fragments arranged by size:
796 574 136 108
NcoI C'CATG_G
Cuts at: 0 1451 1560 1614
Size: 1451 109 54
Fragments arranged by size:
1451 109 54
NgoMIV G'CCGG_C
Cuts at: 0 186 464 1614
Size: 186 278 1150
Fragments arranged by size:
1150 278 186
NsiI A_TGCA'T
Cuts at: 0 1288 1614
Size: 1288 326
NspI r_CATG'y
Cuts at: 0 1575 1614
Size: 1575 39
PasI CC'CwG_GG
Cuts at: 0 702 1614
Size: 702 912
PflMI CCAn_nnn'nTGG
Cuts at: 0 1062 1614
Size: 1062 552
PfoI T'CCnGG_A
Cuts at: 0 588 1614
Size: 588 1026
PpuMI rG'GwC_Cy
Cuts at: 0 86 127 163 597 940 1614
Size: 86 41 36 434 343 674
Fragments arranged by size:
674 434 343 86 41 36
PspOMI G'GGCC_C
Cuts at: 0 142 748 965 1614
Size: 142 606 217 649
Fragments arranged by size:
649 606 217 142
PstI C_TGCA'G
Cuts at: 0 475 1614
Size: 475 1139
PvuII CAG'CTG
Cuts at: 0 1198 1506 1614
Size: 1198 308 108
Fragments arranged by size:
1198 308 108
SacI G_AGCT'C
Cuts at: 0 620 720 1614
Size: 620 100 894
Fragments arranged by size:
894 620 100
SacII CC_GC'GG
Cuts at: 0 272 659 894 1614
Size: 272 387 235 720
Fragments arranged by size:
720 387 272 235
SanDI GG'GwC_CC
Cuts at: 0 86 597 1614
Size: 86 511 1017
Fragments arranged by size:
1017 511 86
SexAI A'CCwGG_T
Cuts at: 0 936 1614
Size: 936 678
SfcI C'TryA_G
Cuts at: 0 471 1134 1614
Size: 471 663 480
Fragments arranged by size:
663 480 471
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 964 1614
Size: 964 650
SfoI GGC'GCC
Cuts at: 0 109 245 819 1614
Size: 109 136 574 795
Fragments arranged by size:
795 574 136 109
SgrAI Cr'CCGG_yG
Cuts at: 0 868 1614
Size: 868 746
SmaI CCC'GGG
Cuts at: 0 93 193 289 415 964 1614
Size: 93 100 96 126 549 650
Fragments arranged by size:
650 549 126 100 96 93
SrfI GCCC'GGGC
Cuts at: 0 964 1614
Size: 964 650
StyI C'CwwG_G
Cuts at: 0 130 752 1451 1560 1614
Size: 130 622 699 109 54
Fragments arranged by size:
699 622 130 109 54
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 650 1096 1614
Size: 650 446 518
Fragments arranged by size:
650 518 446
TatI w'GTAC_w
Cuts at: 0 1154 1614
Size: 1154 460
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 30 1614
Size: 30 1584
XmaI C'CCGG_G
Cuts at: 0 91 191 287 413 962 1614
Size: 91 100 96 126 549 652
Fragments arranged by size:
652 549 126 100 96 91
Enzymes that do cut:
AccI Acc65I AgeI AlfI AlwNI ApaI AvaI AvrII
BanI BanII BbeI BbvCI BciVI BfrBI BglI BlpI
Bme1580I BmrI BpmI Bpu10I BsaHI BsaWI BsaXI BseRI
BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspEI BsrBI BsrDI
BsrFI BstAPI BstYI BstZ17I BtgI DraIII EaeI EagI
EciI EcoICRI Eco57MI EcoO109I EcoRV HaeII KasI KpnI
MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV
NsiI NspI PasI PflMI PfoI PpuMI PspOMI PstI
PvuII SacI SacII SanDI SexAI SfcI SfiI SfoI
SgrAI SmaI SrfI StyI TaqII TatI XcmI XmaI
Enzymes that do not cut:
AarI AatII AclI AcuI AfeI AflII AflIII AhdI
AleI AloI ApaLI ApoI AscI AseI AsiSI BaeI
BamHI BbsI BcgI BclI BglII BmgBI BmtI BplI
BpuEI BsaI BsaAI BsaBI BseYI BsiWI BsmI BspHI
BspMI BsrGI BssHII BssSI BstBI BstEII BstXI Bsu36I
BtgZI BtsI ClaI CspCI DraI DrdI EarI EcoNI
EcoRI FalI FseI FspI FspAI Hin4I HincII HindIII
HpaI MfeI MluI NdeI NheI NotI NruI PacI
PciI PmeI PmlI PpiI PshAI PsiI PspXI PsrI
PvuI RsrII SalI SapI SbfI ScaI SgfI SmlI
SnaBI SpeI SphI SspI StuI SwaI TstI Tth111I
XbaI XhoI XmnI ZraI