Restriction map for
pFN21AE1367
(Linear) (Six-base) MAPSORT of: KIEE1367.seq Check: 6062 from: 1 to: 1110
KIEE1367.orf
With 182 enzymes: SgfI *
July 15, 2010 18:37 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 207 1084 1110
Size: 207 877 26
Fragments arranged by size:
877 207 26
AlwNI CAG_nnn'CTG
Cuts at: 0 627 777 1062 1110
Size: 627 150 285 48
Fragments arranged by size:
627 285 150 48
ApaI G_GGCC'C
Cuts at: 0 69 1110
Size: 69 1041
ApoI r'AATT_y
Cuts at: 0 896 931 1110
Size: 896 35 179
Fragments arranged by size:
896 179 35
AvaI C'yCGr_G
Cuts at: 0 79 1110
Size: 79 1031
BanI G'GyrC_C
Cuts at: 0 49 373 651 1110
Size: 49 324 278 459
Fragments arranged by size:
459 324 278 49
BanII G_rGCy'C
Cuts at: 0 69 295 421 1110
Size: 69 226 126 689
Fragments arranged by size:
689 226 126 69
BbeI G_GCGC'C
Cuts at: 0 53 1110
Size: 53 1057
BbsI GAAGACnn'nnnn_
Cuts at: 0 149 1110
Size: 149 961
BbvCI CC'TCA_GC
Cuts at: 0 25 1110
Size: 25 1085
BglI GCCn_nnn'nGGC
Cuts at: 0 502 826 1110
Size: 502 324 284
Fragments arranged by size:
502 324 284
Bme1580I G_kGCm'C
Cuts at: 0 69 1110
Size: 69 1041
BmrI ACTGGGnnnn_n'
Cuts at: 0 167 194 615 787 1110
Size: 167 27 421 172 323
Fragments arranged by size:
421 323 172 167 27
BmtI G_CTAG'C
Cuts at: 0 115 676 1110
Size: 115 561 434
Fragments arranged by size:
561 434 115
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 692 1110
Size: 692 418
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 443 649 800 1110
Size: 443 206 151 310
Fragments arranged by size:
443 310 206 151
Bpu10I CC'TnA_GC
Cuts at: 0 25 1110
Size: 25 1085
BsaI GGTCTCn'nnnn_
Cuts at: 0 221 421 435 1110
Size: 221 200 14 675
Fragments arranged by size:
675 221 200 14
BsaHI Gr'CG_yC
Cuts at: 0 50 1110
Size: 50 1060
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 798 828 1110
Size: 798 30 282
Fragments arranged by size:
798 282 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 70 271 1110
Size: 70 201 839
Fragments arranged by size:
839 201 70
BseYI C'CCAG_C
Cuts at: 0 92 286 1110
Size: 92 194 824
Fragments arranged by size:
824 194 92
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 946 1110
Size: 946 164
BsiEI CG_ry'CG
Cuts at: 0 89 1110
Size: 89 1021
BsmI GAATG_Cn'
Cuts at: 0 522 1110
Size: 522 588
Bsp1286I G_dGCh'C
Cuts at: 0 69 295 421 1110
Size: 69 226 126 689
Fragments arranged by size:
689 226 126 69
BspHI T'CATG_A
Cuts at: 0 237 1016 1110
Size: 237 779 94
Fragments arranged by size:
779 237 94
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1085 1110
Size: 1085 25
BsrFI r'CCGG_y
Cuts at: 0 88 1110
Size: 88 1022
BstXI CCAn_nnnn'nTGG
Cuts at: 0 351 1110
Size: 351 759
BtgI C'CryG_G
Cuts at: 0 992 1110
Size: 992 118
BtsI GCAGTG_nn'
Cuts at: 0 785 1110
Size: 785 325
DraI TTT'AAA
Cuts at: 0 369 1110
Size: 369 741
EaeI y'GGCC_r
Cuts at: 0 86 985 1110
Size: 86 899 125
Fragments arranged by size:
899 125 86
EagI C'GGCC_G
Cuts at: 0 86 1110
Size: 86 1024
EarI CTCTTCn'nnn_
Cuts at: 0 257 482 556 858 973 1110
Size: 257 225 74 302 115 137
Fragments arranged by size:
302 257 225 137 115 74
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 22 1110
Size: 22 1088
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 207 443 649 800 1084 1110
Size: 207 236 206 151 284 26
Fragments arranged by size:
284 236 207 206 151 26
HaeII r_GCGC'y
Cuts at: 0 25 53 1110
Size: 25 28 1057
Fragments arranged by size:
1057 28 25
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 475 507 956 988 1110
Size: 475 32 449 32 122
Fragments arranged by size:
475 449 122 32 32
HincII GTy'rAC
Cuts at: 0 1106 1110
Size: 1106 4
HpaI GTT'AAC
Cuts at: 0 1106 1110
Size: 1106 4
KasI G'GCGC_C
Cuts at: 0 49 1110
Size: 49 1061
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 987 1110
Size: 987 123
MspA1I CmG'CkG
Cuts at: 0 1101 1110
Size: 1101 9
NaeI GCC'GGC
Cuts at: 0 90 1110
Size: 90 1020
NarI GG'CG_CC
Cuts at: 0 50 1110
Size: 50 1060
NcoI C'CATG_G
Cuts at: 0 992 1110
Size: 992 118
NgoMIV G'CCGG_C
Cuts at: 0 88 1110
Size: 88 1022
NheI G'CTAG_C
Cuts at: 0 111 672 1110
Size: 111 561 438
Fragments arranged by size:
561 438 111
PflMI CCAn_nnn'nTGG
Cuts at: 0 627 1110
Size: 627 483
PspOMI G'GGCC_C
Cuts at: 0 65 1110
Size: 65 1045
SapI GCTCTTCn'nnn_
Cuts at: 0 858 1110
Size: 858 252
SfcI C'TryA_G
Cuts at: 0 392 1110
Size: 392 718
SfoI GGC'GCC
Cuts at: 0 51 1110
Size: 51 1059
SmlI C'TyrA_G
Cuts at: 0 79 1110
Size: 79 1031
StyI C'CwwG_G
Cuts at: 0 468 992 1110
Size: 468 524 118
Fragments arranged by size:
524 468 118
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 351 1110
Size: 351 759
XhoI C'TCGA_G
Cuts at: 0 79 1110
Size: 79 1031
Enzymes that do cut:
AcuI AlwNI ApaI ApoI AvaI BanI BanII BbeI
BbsI BbvCI BglI Bme1580I BmrI BmtI BplI BpmI
Bpu10I BsaI BsaHI BsaXI BseRI BseYI BsgI BsiEI
BsmI Bsp1286I BspHI BspMI BsrFI BstXI BtgI BtsI
DraI EaeI EagI EarI EciI Eco57MI HaeII Hin4I
HincII HpaI KasI MmeI MspA1I NaeI NarI NcoI
NgoMIV NheI PflMI PspOMI SapI SfcI SfoI SmlI
StyI XcmI XhoI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI ApaLI AscI AseI
AsiSI AvrII BaeI BamHI BcgI BciVI BclI BfrBI
BglII BlpI BmgBI BpuEI BsaAI BsaBI BsaWI BsiHKAI
BsiWI BsmBI BspEI BsrBI BsrDI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstYI BstZ17I Bsu36I BtgZI ClaI
CspCI DraIII DrdI EcoICRI EcoNI EcoO109I EcoRI EcoRV
FalI FseI FspI FspAI HindIII KpnI MfeI MluI
MscI MslI NdeI NotI NruI NsiI NspI PacI
PasI PciI PfoI PmeI PmlI PpiI PpuMI PshAI
PsiI PspXI PsrI PstI PvuI PvuII RsrII SacI
SacII SalI SanDI SbfI ScaI SexAI SfiI SgfI
SgrAI SmaI SnaBI SpeI SphI SrfI SspI StuI
SwaI TaqII TatI TstI Tth111I XbaI XmaI XmnI
ZraI