Restriction map for
pFN21AE1366
(Linear) (Six-base) MAPSORT of: KIEE1366.seq Check: 9079 from: 1 to: 891
KIEE1366.orf
With 182 enzymes: SgfI *
July 15, 2010 18:37 ..
Acc65I G'GTAC_C
Cuts at: 0 254 891
Size: 254 637
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 75 891
Size: 75 816
ApaI G_GGCC'C
Cuts at: 0 118 891
Size: 118 773
ApoI r'AATT_y
Cuts at: 0 15 357 425 456 891
Size: 15 342 68 31 435
Fragments arranged by size:
435 342 68 31 15
BanI G'GyrC_C
Cuts at: 0 254 891
Size: 254 637
BanII G_rGCy'C
Cuts at: 0 118 182 471 891
Size: 118 64 289 420
Fragments arranged by size:
420 289 118 64
BbsI GAAGACnn'nnnn_
Cuts at: 0 95 196 785 891
Size: 95 101 589 106
Fragments arranged by size:
589 106 101 95
Bme1580I G_kGCm'C
Cuts at: 0 118 891
Size: 118 773
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 336 543 891
Size: 336 207 348
Fragments arranged by size:
348 336 207
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 306 645 891
Size: 306 339 246
Fragments arranged by size:
339 306 246
BsaBI GATnn'nnATC
Cuts at: 0 592 891
Size: 592 299
BsaWI w'CCGG_w
Cuts at: 0 289 891
Size: 289 602
BseYI C'CCAG_C
Cuts at: 0 216 891
Size: 216 675
BsiHKAI G_wGCw'C
Cuts at: 0 471 661 891
Size: 471 190 230
Fragments arranged by size:
471 230 190
BsmBI CGTCTCn'nnnn_
Cuts at: 0 820 891
Size: 820 71
Bsp1286I G_dGCh'C
Cuts at: 0 118 182 471 661 891
Size: 118 64 289 190 230
Fragments arranged by size:
289 230 190 118 64
BspEI T'CCGG_A
Cuts at: 0 289 891
Size: 289 602
BspHI T'CATG_A
Cuts at: 0 134 891
Size: 134 757
BsrDI GCAATG_nn'
Cuts at: 0 188 891
Size: 188 703
BstYI r'GATC_y
Cuts at: 0 224 891
Size: 224 667
DraI TTT'AAA
Cuts at: 0 440 891
Size: 440 451
EarI CTCTTCn'nnn_
Cuts at: 0 94 187 800 891
Size: 94 93 613 91
Fragments arranged by size:
613 94 93 91
EcoICRI GAG'CTC
Cuts at: 0 469 891
Size: 469 422
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 75 306 645 891
Size: 75 231 339 246
Fragments arranged by size:
339 246 231 75
EcoO109I rG'GnC_Cy
Cuts at: 0 55 115 891
Size: 55 60 776
Fragments arranged by size:
776 60 55
EcoRI G'AATT_C
Cuts at: 0 357 891
Size: 357 534
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 844 876 891
Size: 844 32 15
Fragments arranged by size:
844 32 15
HindIII A'AGCT_T
Cuts at: 0 126 891
Size: 126 765
KpnI G_GTAC'C
Cuts at: 0 258 891
Size: 258 633
MslI CAynn'nnrTG
Cuts at: 0 241 891
Size: 241 650
PasI CC'CwG_GG
Cuts at: 0 195 891
Size: 195 696
PfoI T'CCnGG_A
Cuts at: 0 322 608 891
Size: 322 286 283
Fragments arranged by size:
322 286 283
PspOMI G'GGCC_C
Cuts at: 0 114 891
Size: 114 777
SacI G_AGCT'C
Cuts at: 0 471 891
Size: 471 420
SapI GCTCTTCn'nnn_
Cuts at: 0 187 891
Size: 187 704
SspI AAT'ATT
Cuts at: 0 420 891
Size: 420 471
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 24 249 891
Size: 24 225 642
Fragments arranged by size:
642 225 24
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 172 330 891
Size: 172 158 561
Fragments arranged by size:
561 172 158
XmnI GAAnn'nnTTC
Cuts at: 0 791 891
Size: 791 100
Enzymes that do cut:
Acc65I AcuI ApaI ApoI BanI BanII BbsI Bme1580I
BplI BpmI BsaBI BsaWI BseYI BsiHKAI BsmBI Bsp1286I
BspEI BspHI BsrDI BstYI DraI EarI EcoICRI Eco57MI
EcoO109I EcoRI Hin4I HindIII KpnI MslI PasI PfoI
PspOMI SacI SapI SspI TaqII XcmI XmnI
Enzymes that do not cut:
AarI AatII AccI AclI AfeI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaLI AscI AseI
AsiSI AvaI AvrII BaeI BamHI BbeI BbvCI BcgI
BciVI BclI BfrBI BglI BglII BlpI BmgBI BmrI
BmtI Bpu10I BpuEI BsaI BsaAI BsaHI BsaXI BseRI
BsgI BsiEI BsiWI BsmI BspMI BsrBI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstZ17I Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraIII DrdI EaeI
EagI EciI EcoNI EcoRV FalI FseI FspI FspAI
HaeII HincII HpaI KasI MfeI MluI MmeI MscI
MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI
NruI NsiI NspI PacI PciI PflMI PmeI PmlI
PpiI PpuMI PshAI PsiI PspXI PsrI PstI PvuI
PvuII RsrII SacII SalI SanDI SbfI ScaI SexAI
SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SrfI StuI StyI SwaI TatI TstI
Tth111I XbaI XhoI XmaI ZraI