Restriction map for
pFN21AE1365
(Linear) (Six-base) MAPSORT of: KIEE1365.seq Check: 5280 from: 1 to: 1170
KIEE1365.orf
With 182 enzymes: SgfI *
July 15, 2010 18:37 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 670 1170
Size: 670 500
AatII G_ACGT'C
Cuts at: 0 812 1170
Size: 812 358
AccI GT'mk_AC
Cuts at: 0 119 1170
Size: 119 1051
AfeI AGC'GCT
Cuts at: 0 49 1072 1170
Size: 49 1023 98
Fragments arranged by size:
1023 98 49
AflIII A'CryG_T
Cuts at: 0 77 857 1170
Size: 77 780 313
Fragments arranged by size:
780 313 77
AhdI GACnn_n'nnGTC
Cuts at: 0 732 1170
Size: 732 438
AleI CACnn'nnGTG
Cuts at: 0 715 1170
Size: 715 455
AlwNI CAG_nnn'CTG
Cuts at: 0 102 1170
Size: 102 1068
AvaI C'yCGr_G
Cuts at: 0 510 628 742 1036 1048 1170
Size: 510 118 114 294 12 122
Fragments arranged by size:
510 294 122 118 114 12
BamHI G'GATC_C
Cuts at: 0 611 1170
Size: 611 559
BanI G'GyrC_C
Cuts at: 0 891 1092 1170
Size: 891 201 78
Fragments arranged by size:
891 201 78
BanII G_rGCy'C
Cuts at: 0 517 635 671 1036 1045 1170
Size: 517 118 36 365 9 125
Fragments arranged by size:
517 365 125 118 36 9
BbeI G_GCGC'C
Cuts at: 0 895 1170
Size: 895 275
BbsI GAAGACnn'nnnn_
Cuts at: 0 511 665 1170
Size: 511 154 505
Fragments arranged by size:
511 505 154
BbvCI CC'TCA_GC
Cuts at: 0 955 1170
Size: 955 215
BglI GCCn_nnn'nGGC
Cuts at: 0 703 1101 1170
Size: 703 398 69
Fragments arranged by size:
703 398 69
BglII A'GATC_T
Cuts at: 0 137 320 1170
Size: 137 183 850
Fragments arranged by size:
850 183 137
Bme1580I G_kGCm'C
Cuts at: 0 277 1097 1170
Size: 277 820 73
Fragments arranged by size:
820 277 73
BmgBI CAC'GTC
Cuts at: 0 543 858 1170
Size: 543 315 312
Fragments arranged by size:
543 315 312
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 525 1170
Size: 525 645
Bpu10I CC'TnA_GC
Cuts at: 0 665 955 1170
Size: 665 290 215
Fragments arranged by size:
665 290 215
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 358 1170
Size: 358 812
BsaHI Gr'CG_yC
Cuts at: 0 809 892 965 1005 1159 1170
Size: 809 83 73 40 154 11
Fragments arranged by size:
809 154 83 73 40 11
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 331 1066 1069 1170
Size: 331 735 3 101
Fragments arranged by size:
735 331 101 3
BseYI C'CCAG_C
Cuts at: 0 33 58 225 615 1170
Size: 33 25 167 390 555
Fragments arranged by size:
555 390 167 33 25
BsiEI CG_ry'CG
Cuts at: 0 943 1021 1105 1170
Size: 943 78 84 65
Fragments arranged by size:
943 84 78 65
BsiHKAI G_wGCw'C
Cuts at: 0 1144 1170
Size: 1144 26
BsmBI CGTCTCn'nnnn_
Cuts at: 0 646 1170
Size: 646 524
Bsp1286I G_dGCh'C
Cuts at: 0 277 517 635 671 1036 1045 1097 1144
Size: 277 240 118 36 365 9 52 47
Cuts at: 1144 1170
Size: 26
Fragments arranged by size:
365 277 240 118 52 47 36 26
9
BspHI T'CATG_A
Cuts at: 0 302 1170
Size: 302 868
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 670 845 893 1170
Size: 670 175 48 277
Fragments arranged by size:
670 277 175 48
BsrBI CCG'CTC
Cuts at: 0 572 1047 1059 1170
Size: 572 475 12 111
Fragments arranged by size:
572 475 111 12
BsrDI GCAATG_nn'
Cuts at: 0 488 1170
Size: 488 682
BsrFI r'CCGG_y
Cuts at: 0 122 457 1086 1170
Size: 122 335 629 84
Fragments arranged by size:
629 335 122 84
BsrGI T'GTAC_A
Cuts at: 0 74 1170
Size: 74 1096
BstYI r'GATC_y
Cuts at: 0 137 320 611 1170
Size: 137 183 291 559
Fragments arranged by size:
559 291 183 137
BtgI C'CryG_G
Cuts at: 0 383 533 1099 1170
Size: 383 150 566 71
Fragments arranged by size:
566 383 150 71
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 9 1170
Size: 9 1161
BtsI GCAGTG_nn'
Cuts at: 0 710 1170
Size: 710 460
DraIII CAC_nnn'GTG
Cuts at: 0 72 1170
Size: 72 1098
EaeI y'GGCC_r
Cuts at: 0 1018 1102 1170
Size: 1018 84 68
Fragments arranged by size:
1018 84 68
EagI C'GGCC_G
Cuts at: 0 1018 1102 1170
Size: 1018 84 68
Fragments arranged by size:
1018 84 68
EarI CTCTTCn'nnn_
Cuts at: 0 556 1170
Size: 556 614
EcoNI CCTnn'n_nnAGG
Cuts at: 0 742 958 1170
Size: 742 216 212
Fragments arranged by size:
742 216 212
EcoO109I rG'GnC_Cy
Cuts at: 0 99 1170
Size: 99 1071
HaeII r_GCGC'y
Cuts at: 0 51 129 895 954 1074 1112 1170
Size: 51 78 766 59 120 38 58
Fragments arranged by size:
766 120 78 59 58 51 38
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 596 628 718 750 1170
Size: 596 32 90 32 420
Fragments arranged by size:
596 420 90 32 32
KasI G'GCGC_C
Cuts at: 0 891 1170
Size: 891 279
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 86 1170
Size: 86 1084
MslI CAynn'nnrTG
Cuts at: 0 301 715 1170
Size: 301 414 455
Fragments arranged by size:
455 414 301
MspA1I CmG'CkG
Cuts at: 0 252 329 939 1101 1130 1170
Size: 252 77 610 162 29 40
Fragments arranged by size:
610 252 162 77 40 29
NaeI GCC'GGC
Cuts at: 0 1088 1170
Size: 1088 82
NarI GG'CG_CC
Cuts at: 0 892 1170
Size: 892 278
NgoMIV G'CCGG_C
Cuts at: 0 1086 1170
Size: 1086 84
NspI r_CATG'y
Cuts at: 0 81 1170
Size: 81 1089
PciI A'CATG_T
Cuts at: 0 77 1170
Size: 77 1093
PfoI T'CCnGG_A
Cuts at: 0 776 1170
Size: 776 394
PpuMI rG'GwC_Cy
Cuts at: 0 99 1170
Size: 99 1071
PspXI vC'TCGA_Gb
Cuts at: 0 628 742 1048 1170
Size: 628 114 306 122
Fragments arranged by size:
628 306 122 114
PstI C_TGCA'G
Cuts at: 0 295 476 998 1135 1170
Size: 295 181 522 137 35
Fragments arranged by size:
522 295 181 137 35
SacII CC_GC'GG
Cuts at: 0 1102 1170
Size: 1102 68
SapI GCTCTTCn'nnn_
Cuts at: 0 556 1170
Size: 556 614
ScaI AGT'ACT
Cuts at: 0 289 1170
Size: 289 881
SfcI C'TryA_G
Cuts at: 0 291 472 994 1131 1170
Size: 291 181 522 137 39
Fragments arranged by size:
522 291 181 137 39
SfoI GGC'GCC
Cuts at: 0 893 1170
Size: 893 277
SmaI CCC'GGG
Cuts at: 0 1038 1170
Size: 1038 132
SmlI C'TyrA_G
Cuts at: 0 373 628 742 1048 1170
Size: 373 255 114 306 122
Fragments arranged by size:
373 306 255 122 114
StuI AGG'CCT
Cuts at: 0 706 1170
Size: 706 464
TatI w'GTAC_w
Cuts at: 0 74 287 1170
Size: 74 213 883
Fragments arranged by size:
883 213 74
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 772 804 1170
Size: 772 32 366
Fragments arranged by size:
772 366 32
Tth111I GACn'n_nGTC
Cuts at: 0 808 1170
Size: 808 362
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 233 540 1152 1170
Size: 233 307 612 18
Fragments arranged by size:
612 307 233 18
XhoI C'TCGA_G
Cuts at: 0 628 742 1048 1170
Size: 628 114 306 122
Fragments arranged by size:
628 306 122 114
XmaI C'CCGG_G
Cuts at: 0 1036 1170
Size: 1036 134
XmnI GAAnn'nnTTC
Cuts at: 0 49 1170
Size: 49 1121
ZraI GAC'GTC
Cuts at: 0 810 1170
Size: 810 360
Enzymes that do cut:
AarI AatII AccI AfeI AflIII AhdI AleI AlwNI
AvaI BamHI BanI BanII BbeI BbsI BbvCI BglI
BglII Bme1580I BmgBI BplI Bpu10I BpuEI BsaHI BseRI
BseYI BsiEI BsiHKAI BsmBI Bsp1286I BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BstYI BtgI BtgZI BtsI DraIII
EaeI EagI EarI EcoNI EcoO109I HaeII Hin4I KasI
MmeI MslI MspA1I NaeI NarI NgoMIV NspI PciI
PfoI PpuMI PspXI PstI SacII SapI ScaI SfcI
SfoI SmaI SmlI StuI TatI TstI Tth111I XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
Acc65I AclI AcuI AflII AgeI AlfI AloI ApaI
ApaLI ApoI AscI AseI AsiSI AvrII BaeI BcgI
BciVI BclI BfrBI BlpI BmrI BmtI BpmI BsaI
BsaAI BsaBI BsaWI BsaXI BsgI BsiWI BsmI BspEI
BssHII BssSI BstAPI BstBI BstEII BstXI BstZ17I Bsu36I
ClaI CspCI DraI DrdI EciI EcoICRI Eco57MI EcoRI
EcoRV FalI FseI FspI FspAI HincII HindIII HpaI
KpnI MfeI MluI MscI NcoI NdeI NheI NotI
NruI NsiI PacI PasI PflMI PmeI PmlI PpiI
PshAI PsiI PspOMI PsrI PvuI PvuII RsrII SacI
SalI SanDI SbfI SexAI SfiI SgfI SgrAI SnaBI
SpeI SphI SrfI SspI StyI SwaI TaqII XbaI