Restriction map for
pFN21AE1364
(Linear) (Six-base) MAPSORT of: KIEE1364.seq Check: 404 from: 1 to: 909
KIEE1364.orf
With 182 enzymes: SgfI *
July 15, 2010 18:37 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 90 401 885 909
Size: 90 311 484 24
Fragments arranged by size:
484 311 90 24
AflII C'TTAA_G
Cuts at: 0 423 909
Size: 423 486
AgeI A'CCGG_T
Cuts at: 0 80 909
Size: 80 829
AleI CACnn'nnGTG
Cuts at: 0 790 909
Size: 790 119
ApaI G_GGCC'C
Cuts at: 0 602 909
Size: 602 307
ApoI r'AATT_y
Cuts at: 0 186 909
Size: 186 723
AvaI C'yCGr_G
Cuts at: 0 152 594 909
Size: 152 442 315
Fragments arranged by size:
442 315 152
BanII G_rGCy'C
Cuts at: 0 602 703 909
Size: 602 101 206
Fragments arranged by size:
602 206 101
BfrBI ATG'CAT
Cuts at: 0 209 909
Size: 209 700
BglII A'GATC_T
Cuts at: 0 360 909
Size: 360 549
BlpI GC'TnA_GC
Cuts at: 0 71 909
Size: 71 838
Bme1580I G_kGCm'C
Cuts at: 0 602 909
Size: 602 307
BmgBI CAC'GTC
Cuts at: 0 321 909
Size: 321 588
BmrI ACTGGGnnnn_n'
Cuts at: 0 634 909
Size: 634 275
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 766 909
Size: 766 143
BsaI GGTCTCn'nnnn_
Cuts at: 0 879 909
Size: 879 30
BsaBI GATnn'nnATC
Cuts at: 0 620 909
Size: 620 289
BsaWI w'CCGG_w
Cuts at: 0 80 909
Size: 80 829
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 711 763 909
Size: 711 52 146
Fragments arranged by size:
711 146 52
BseYI C'CCAG_C
Cuts at: 0 198 909
Size: 198 711
BsiHKAI G_wGCw'C
Cuts at: 0 755 909
Size: 755 154
Bsp1286I G_dGCh'C
Cuts at: 0 602 703 755 909
Size: 602 101 52 154
Fragments arranged by size:
602 154 101 52
BsrFI r'CCGG_y
Cuts at: 0 80 909
Size: 80 829
BssSI C'ACGA_G
Cuts at: 0 690 909
Size: 690 219
BstAPI GCAn_nnn'nTGC
Cuts at: 0 363 909
Size: 363 546
BstBI TT'CG_AA
Cuts at: 0 258 909
Size: 258 651
BstYI r'GATC_y
Cuts at: 0 360 635 779 860 909
Size: 360 275 144 81 49
Fragments arranged by size:
360 275 144 81 49
BtgI C'CryG_G
Cuts at: 0 429 671 909
Size: 429 242 238
Fragments arranged by size:
429 242 238
DrdI GACnn_nn'nnGTC
Cuts at: 0 325 909
Size: 325 584
EarI CTCTTCn'nnn_
Cuts at: 0 20 109 295 377 544 664 702 909
Size: 20 89 186 82 167 120 38 207
Fragments arranged by size:
207 186 167 120 89 82 38 20
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 90 401 766 885 909
Size: 90 311 365 119 24
Fragments arranged by size:
365 311 119 90 24
EcoO109I rG'GnC_Cy
Cuts at: 0 598 909
Size: 598 311
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 382 909
Size: 382 527
HaeII r_GCGC'y
Cuts at: 0 289 909
Size: 289 620
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 70 102 345 377 620 652 811 843
Size: 70 32 243 32 243 32 159 32
Cuts at: 843 909
Size: 66
Fragments arranged by size:
243 243 159 70 66 32 32 32
32
HindIII A'AGCT_T
Cuts at: 0 374 909
Size: 374 535
MslI CAynn'nnrTG
Cuts at: 0 395 790 845 909
Size: 395 395 55 64
Fragments arranged by size:
395 395 64 55
NcoI C'CATG_G
Cuts at: 0 429 909
Size: 429 480
NsiI A_TGCA'T
Cuts at: 0 211 909
Size: 211 698
PfoI T'CCnGG_A
Cuts at: 0 743 909
Size: 743 166
PspOMI G'GGCC_C
Cuts at: 0 598 909
Size: 598 311
SapI GCTCTTCn'nnn_
Cuts at: 0 20 295 909
Size: 20 275 614
Fragments arranged by size:
614 275 20
SmaI CCC'GGG
Cuts at: 0 154 909
Size: 154 755
SmlI C'TyrA_G
Cuts at: 0 423 909
Size: 423 486
StyI C'CwwG_G
Cuts at: 0 429 887 909
Size: 429 458 22
Fragments arranged by size:
458 429 22
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 173 580 909
Size: 173 407 329
Fragments arranged by size:
407 329 173
TatI w'GTAC_w
Cuts at: 0 176 909
Size: 176 733
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 447 479 909
Size: 447 32 430
Fragments arranged by size:
447 430 32
XmaI C'CCGG_G
Cuts at: 0 152 909
Size: 152 757
Enzymes that do cut:
AcuI AflII AgeI AleI ApaI ApoI AvaI BanII
BfrBI BglII BlpI Bme1580I BmgBI BmrI BpmI BsaI
BsaBI BsaWI BseRI BseYI BsiHKAI Bsp1286I BsrFI BssSI
BstAPI BstBI BstYI BtgI DrdI EarI Eco57MI EcoO109I
FalI HaeII Hin4I HindIII MslI NcoI NsiI PfoI
PspOMI SapI SmaI SmlI StyI TaqII TatI TstI
XmaI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflIII AhdI
AlfI AloI AlwNI ApaLI AscI AseI AsiSI AvrII
BaeI BamHI BanI BbeI BbsI BbvCI BcgI BciVI
BclI BglI BmtI BplI Bpu10I BpuEI BsaAI BsaHI
BsaXI BsgI BsiEI BsiWI BsmI BsmBI BspEI BspHI
BspMI BsrBI BsrDI BsrGI BssHII BstEII BstXI BstZ17I
Bsu36I BtgZI BtsI ClaI CspCI DraI DraIII EaeI
EagI EciI EcoICRI EcoNI EcoRI EcoRV FseI FspI
FspAI HincII HpaI KasI KpnI MfeI MluI MmeI
MscI MspA1I NaeI NarI NdeI NgoMIV NheI NotI
NruI NspI PacI PasI PciI PflMI PmeI PmlI
PpiI PpuMI PshAI PsiI PspXI PsrI PstI PvuI
PvuII RsrII SacI SacII SalI SanDI SbfI ScaI
SexAI SfcI SfiI SfoI SgfI SgrAI SnaBI SpeI
SphI SrfI SspI StuI SwaI Tth111I XbaI XcmI
XhoI XmnI ZraI