Restriction map for
pFN21AE1363
(Linear) (Six-base) MAPSORT of: KIEE1363.seq Check: 3229 from: 1 to: 1755
KIEE1363.orf
With 182 enzymes: SgfI *
July 15, 2010 18:37 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 247 1755
Size: 247 1508
AflIII A'CryG_T
Cuts at: 0 352 1126 1755
Size: 352 774 629
Fragments arranged by size:
774 629 352
AgeI A'CCGG_T
Cuts at: 0 1062 1755
Size: 1062 693
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 834 866 1755
Size: 834 32 889
Fragments arranged by size:
889 834 32
AlwNI CAG_nnn'CTG
Cuts at: 0 1666 1731 1755
Size: 1666 65 24
Fragments arranged by size:
1666 65 24
ApaLI G'TGCA_C
Cuts at: 0 578 1755
Size: 578 1177
ApoI r'AATT_y
Cuts at: 0 244 843 1163 1700 1755
Size: 244 599 320 537 55
Fragments arranged by size:
599 537 320 244 55
AseI AT'TA_AT
Cuts at: 0 509 1755
Size: 509 1246
BanI G'GyrC_C
Cuts at: 0 3 1094 1527 1755
Size: 3 1091 433 228
Fragments arranged by size:
1091 433 228 3
BbeI G_GCGC'C
Cuts at: 0 7 1755
Size: 7 1748
BciVI GTATCCnnnnn_n'
Cuts at: 0 308 1150 1755
Size: 308 842 605
Fragments arranged by size:
842 605 308
Bme1580I G_kGCm'C
Cuts at: 0 429 582 1099 1755
Size: 429 153 517 656
Fragments arranged by size:
656 517 429 153
BmrI ACTGGGnnnn_n'
Cuts at: 0 356 1755
Size: 356 1399
BmtI G_CTAG'C
Cuts at: 0 719 1755
Size: 719 1036
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1708 1755
Size: 1708 47
BsaHI Gr'CG_yC
Cuts at: 0 4 1755
Size: 4 1751
BsaWI w'CCGG_w
Cuts at: 0 1062 1755
Size: 1062 693
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 158 188 1116 1146 1755
Size: 158 30 928 30 609
Fragments arranged by size:
928 609 158 30 30
BseYI C'CCAG_C
Cuts at: 0 310 1344 1755
Size: 310 1034 411
Fragments arranged by size:
1034 411 310
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 193 1755
Size: 193 1562
BsiHKAI G_wGCw'C
Cuts at: 0 582 1134 1552 1755
Size: 582 552 418 203
Fragments arranged by size:
582 552 418 203
Bsp1286I G_dGCh'C
Cuts at: 0 429 582 1099 1134 1552 1755
Size: 429 153 517 35 418 203
Fragments arranged by size:
517 429 418 203 153 35
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1218 1755
Size: 1218 537
BsrBI CCG'CTC
Cuts at: 0 18 1755
Size: 18 1737
BsrDI GCAATG_nn'
Cuts at: 0 168 438 773 1755
Size: 168 270 335 982
Fragments arranged by size:
982 335 270 168
BsrFI r'CCGG_y
Cuts at: 0 1062 1755
Size: 1062 693
BsrGI T'GTAC_A
Cuts at: 0 47 1755
Size: 47 1708
EarI CTCTTCn'nnn_
Cuts at: 0 1382 1755
Size: 1382 373
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 247 1708 1755
Size: 247 1461 47
Fragments arranged by size:
1461 247 47
EcoNI CCTnn'n_nnAGG
Cuts at: 0 226 1723 1755
Size: 226 1497 32
Fragments arranged by size:
1497 226 32
EcoO109I rG'GnC_Cy
Cuts at: 0 143 1587 1755
Size: 143 1444 168
Fragments arranged by size:
1444 168 143
EcoRI G'AATT_C
Cuts at: 0 244 843 1755
Size: 244 599 912
Fragments arranged by size:
912 599 244
HaeII r_GCGC'y
Cuts at: 0 7 1755
Size: 7 1748
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1192 1224 1755
Size: 1192 32 531
Fragments arranged by size:
1192 531 32
HincII GTy'rAC
Cuts at: 0 438 729 1755
Size: 438 291 1026
Fragments arranged by size:
1026 438 291
HpaI GTT'AAC
Cuts at: 0 438 1755
Size: 438 1317
KasI G'GCGC_C
Cuts at: 0 3 1755
Size: 3 1752
MfeI C'AATT_G
Cuts at: 0 479 1755
Size: 479 1276
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1673 1755
Size: 1673 82
MslI CAynn'nnrTG
Cuts at: 0 1196 1755
Size: 1196 559
MspA1I CmG'CkG
Cuts at: 0 310 1755
Size: 310 1445
NarI GG'CG_CC
Cuts at: 0 4 1755
Size: 4 1751
NheI G'CTAG_C
Cuts at: 0 715 1755
Size: 715 1040
NspI r_CATG'y
Cuts at: 0 356 1130 1755
Size: 356 774 625
Fragments arranged by size:
774 625 356
PciI A'CATG_T
Cuts at: 0 352 1126 1755
Size: 352 774 629
Fragments arranged by size:
774 629 352
PflMI CCAn_nnn'nTGG
Cuts at: 0 817 1731 1755
Size: 817 914 24
Fragments arranged by size:
914 817 24
PfoI T'CCnGG_A
Cuts at: 0 564 1724 1755
Size: 564 1160 31
Fragments arranged by size:
1160 564 31
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 930 962 1755
Size: 930 32 793
Fragments arranged by size:
930 793 32
PpuMI rG'GwC_Cy
Cuts at: 0 143 1587 1755
Size: 143 1444 168
Fragments arranged by size:
1444 168 143
PshAI GACnn'nnGTC
Cuts at: 0 1514 1755
Size: 1514 241
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 749 781 1755
Size: 749 32 974
Fragments arranged by size:
974 749 32
PvuII CAG'CTG
Cuts at: 0 310 1755
Size: 310 1445
SanDI GG'GwC_CC
Cuts at: 0 1587 1755
Size: 1587 168
SfcI C'TryA_G
Cuts at: 0 797 963 1755
Size: 797 166 792
Fragments arranged by size:
797 792 166
SfoI GGC'GCC
Cuts at: 0 5 1755
Size: 5 1750
SspI AAT'ATT
Cuts at: 0 180 1259 1755
Size: 180 1079 496
Fragments arranged by size:
1079 496 180
StyI C'CwwG_G
Cuts at: 0 94 1755
Size: 94 1661
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 267 1755
Size: 267 1488
TatI w'GTAC_w
Cuts at: 0 47 1755
Size: 47 1708
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 75 107 1755
Size: 75 32 1648
Fragments arranged by size:
1648 75 32
XmnI GAAnn'nnTTC
Cuts at: 0 364 1755
Size: 364 1391
Enzymes that do cut:
AcuI AflIII AgeI AloI AlwNI ApaLI ApoI AseI
BanI BbeI BciVI Bme1580I BmrI BmtI BpmI BsaHI
BsaWI BsaXI BseYI BsgI BsiHKAI Bsp1286I BspMI BsrBI
BsrDI BsrFI BsrGI EarI Eco57MI EcoNI EcoO109I EcoRI
HaeII Hin4I HincII HpaI KasI MfeI MmeI MslI
MspA1I NarI NheI NspI PciI PflMI PfoI PpiI
PpuMI PshAI PsrI PvuII SanDI SfcI SfoI SspI
StyI TaqII TatI TstI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AhdI
AleI AlfI ApaI AscI AsiSI AvaI AvrII BaeI
BamHI BanII BbsI BbvCI BcgI BclI BfrBI BglI
BglII BlpI BmgBI BplI Bpu10I BpuEI BsaI BsaAI
BsaBI BseRI BsiEI BsiWI BsmI BsmBI BspEI BspHI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII
DrdI EaeI EagI EciI EcoICRI EcoRV FalI FseI
FspI FspAI HindIII KpnI MluI MscI NaeI NcoI
NdeI NgoMIV NotI NruI NsiI PacI PasI PmeI
PmlI PsiI PspOMI PspXI PstI PvuI RsrII SacI
SacII SalI SapI SbfI ScaI SexAI SfiI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SrfI StuI
SwaI Tth111I XbaI XcmI XhoI XmaI ZraI